The Rep and C1 of Beet curly top Iran virus represent pathogenicity factors and induce hypersensitive response in Nicotiana benthamiana plants
Beet curly top Iran virus (BCTIV) is a member of the genus Becurtovirus (Family Geminiviridae ) with a circular single-strand DNA genome. BCTIV causes leaf curling and vein swelling symptoms in plants. However, the potential pathogenicity factor/s in BCTIV is/are not known. This study presents chara...
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| Veröffentlicht in: | Virus genes 2022-12, Vol.58 (6), p.550-559 |
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| creator | Ebrahimi, Saeideh Eini, Omid Koolivand, Davoud Varrelmann, Mark |
| description | Beet curly top Iran virus
(BCTIV) is a member of the genus
Becurtovirus
(Family
Geminiviridae
) with a circular single-strand DNA genome. BCTIV causes leaf curling and vein swelling symptoms in plants. However, the potential pathogenicity factor/s in BCTIV is/are not known. This study presents characterization of complementary-sense transcripts of BCTIV and the viral factors in directing the pathogenicity and hypersensitive response (HR) in
Nicotiana benthamiana
plants. In both local and systemic infection, splicing of the complementary transcripts of BCTIV was observed. Notably, a small number (8.3%) of transcripts were spliced to produce Rep (C1:C2) transcripts after deletion of 155 nt (position 1892–2046 from BCTIV). Expression of BCTIV genes in
N. benthamiana
using tobacco rattle virus (TRV)-based vector showed that Rep together with C1 are the main pathogenicity factors which cause typical viral leaf curling symptoms. In addition, the V2 caused a mild leaf curling, thickening, and asymmetric leaves, while the V1
,
V3, and C2 had no clear effect on the plant phenotype. Transient expression of individual viral genes showed that both the C1 and Rep trigger a HR response in
N. benthamiana
. The higher expression of HR marker genes, harpin-induced 1 (
Hin1
) and hypersensitivity‐related (
Hsr203JI)
, supported the role of C1 and Rep in HR response in plants. It is concluded that Rep and C1 are the main pathogenicity factors that also trigger HR response in plants. |
| doi_str_mv | 10.1007/s11262-022-01927-3 |
| format | Article |
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(BCTIV) is a member of the genus
Becurtovirus
(Family
Geminiviridae
) with a circular single-strand DNA genome. BCTIV causes leaf curling and vein swelling symptoms in plants. However, the potential pathogenicity factor/s in BCTIV is/are not known. This study presents characterization of complementary-sense transcripts of BCTIV and the viral factors in directing the pathogenicity and hypersensitive response (HR) in
Nicotiana benthamiana
plants. In both local and systemic infection, splicing of the complementary transcripts of BCTIV was observed. Notably, a small number (8.3%) of transcripts were spliced to produce Rep (C1:C2) transcripts after deletion of 155 nt (position 1892–2046 from BCTIV). Expression of BCTIV genes in
N. benthamiana
using tobacco rattle virus (TRV)-based vector showed that Rep together with C1 are the main pathogenicity factors which cause typical viral leaf curling symptoms. In addition, the V2 caused a mild leaf curling, thickening, and asymmetric leaves, while the V1
,
V3, and C2 had no clear effect on the plant phenotype. Transient expression of individual viral genes showed that both the C1 and Rep trigger a HR response in
N. benthamiana
. The higher expression of HR marker genes, harpin-induced 1 (
Hin1
) and hypersensitivity‐related (
Hsr203JI)
, supported the role of C1 and Rep in HR response in plants. It is concluded that Rep and C1 are the main pathogenicity factors that also trigger HR response in plants.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-022-01927-3</identifier><identifier>PMID: 35960462</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Amino acids ; Apoptosis ; Beet curly top Iran virus ; Beta vulgaris ; Biomedical and Life Sciences ; Biomedicine ; Cell death ; Cloning ; Disseminated infection ; DNA ; Geminiviridae ; Gene expression ; genome ; Genomes ; Hypersensitive response ; Iran ; Leaves ; Medical Microbiology ; Nicotiana ; Nicotiana benthamiana ; Original Paper ; Pathogenicity ; phenotype ; Phenotypes ; Plant Diseases ; Plant Sciences ; Plant virus diseases ; Plants ; Plasmids ; Proteins ; Tobacco ; Tobacco rattle virus ; Virology ; Virulence Factors - genetics ; Viruses</subject><ispartof>Virus genes, 2022-12, Vol.58 (6), p.550-559</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-ce0b5f3af7d581c306e207b9f49bff96ee156e512d9afd6da8858b093a98f85d3</citedby><cites>FETCH-LOGICAL-c338t-ce0b5f3af7d581c306e207b9f49bff96ee156e512d9afd6da8858b093a98f85d3</cites><orcidid>0000-0001-9968-0525</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11262-022-01927-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11262-022-01927-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35960462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ebrahimi, Saeideh</creatorcontrib><creatorcontrib>Eini, Omid</creatorcontrib><creatorcontrib>Koolivand, Davoud</creatorcontrib><creatorcontrib>Varrelmann, Mark</creatorcontrib><title>The Rep and C1 of Beet curly top Iran virus represent pathogenicity factors and induce hypersensitive response in Nicotiana benthamiana plants</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><addtitle>Virus Genes</addtitle><description>Beet curly top Iran virus
(BCTIV) is a member of the genus
Becurtovirus
(Family
Geminiviridae
) with a circular single-strand DNA genome. BCTIV causes leaf curling and vein swelling symptoms in plants. However, the potential pathogenicity factor/s in BCTIV is/are not known. This study presents characterization of complementary-sense transcripts of BCTIV and the viral factors in directing the pathogenicity and hypersensitive response (HR) in
Nicotiana benthamiana
plants. In both local and systemic infection, splicing of the complementary transcripts of BCTIV was observed. Notably, a small number (8.3%) of transcripts were spliced to produce Rep (C1:C2) transcripts after deletion of 155 nt (position 1892–2046 from BCTIV). Expression of BCTIV genes in
N. benthamiana
using tobacco rattle virus (TRV)-based vector showed that Rep together with C1 are the main pathogenicity factors which cause typical viral leaf curling symptoms. In addition, the V2 caused a mild leaf curling, thickening, and asymmetric leaves, while the V1
,
V3, and C2 had no clear effect on the plant phenotype. Transient expression of individual viral genes showed that both the C1 and Rep trigger a HR response in
N. benthamiana
. The higher expression of HR marker genes, harpin-induced 1 (
Hin1
) and hypersensitivity‐related (
Hsr203JI)
, supported the role of C1 and Rep in HR response in plants. It is concluded that Rep and C1 are the main pathogenicity factors that also trigger HR response in plants.</description><subject>Amino acids</subject><subject>Apoptosis</subject><subject>Beet curly top Iran virus</subject><subject>Beta vulgaris</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell death</subject><subject>Cloning</subject><subject>Disseminated infection</subject><subject>DNA</subject><subject>Geminiviridae</subject><subject>Gene expression</subject><subject>genome</subject><subject>Genomes</subject><subject>Hypersensitive response</subject><subject>Iran</subject><subject>Leaves</subject><subject>Medical Microbiology</subject><subject>Nicotiana</subject><subject>Nicotiana benthamiana</subject><subject>Original Paper</subject><subject>Pathogenicity</subject><subject>phenotype</subject><subject>Phenotypes</subject><subject>Plant Diseases</subject><subject>Plant Sciences</subject><subject>Plant virus diseases</subject><subject>Plants</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Tobacco</subject><subject>Tobacco rattle virus</subject><subject>Virology</subject><subject>Virulence Factors - genetics</subject><subject>Viruses</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkd9qFDEUxoModq2-gBcS8Mab0fyZzCSXulRbKApSwbuQyZx0U3aTmGQK-xI-s-luq-CFwgkncH7fdxI-hF5S8pYSMr4rlLKBdYS1QxUbO_4IragYWadU__0xWhHFSCfFoE7Qs1JuCCFSsv4pOuFCDaQf2Ar9vNoA_goJmzDjNcXR4Q8AFdslb_e4xoQvsgn41uel4AwpQ4FQcTJ1E68heOvrHjtja8zl4OHDvFjAm32C3NDiq7-FpiwphgJtjD97G6s3weCpWW3M7nBPWxNqeY6eOLMt8OK-n6JvH8-u1ufd5ZdPF-v3l53lXNbOApmE48aNs5DUcjIAI-OkXK8m59QAQMUAgrJZGTcPs5FSyIkobpR0Usz8FL05-qYcfyxQqt75YmHbHgFxKZrJXklJeav_oiNhVDLO-oa-_gu9iUsO7SON4lRxIRVvFDtSNsdSMjidst-ZvNeU6Ltg9TFY3YLVh2D1nejVvfUy7WD-LXlIsgH8CJQ2CteQ_-z-h-0vtaiv5w</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Ebrahimi, Saeideh</creator><creator>Eini, Omid</creator><creator>Koolivand, Davoud</creator><creator>Varrelmann, Mark</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-9968-0525</orcidid></search><sort><creationdate>20221201</creationdate><title>The Rep and C1 of Beet curly top Iran virus represent pathogenicity factors and induce hypersensitive response in Nicotiana benthamiana plants</title><author>Ebrahimi, Saeideh ; Eini, Omid ; Koolivand, Davoud ; Varrelmann, Mark</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-ce0b5f3af7d581c306e207b9f49bff96ee156e512d9afd6da8858b093a98f85d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amino acids</topic><topic>Apoptosis</topic><topic>Beet curly top Iran virus</topic><topic>Beta vulgaris</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell death</topic><topic>Cloning</topic><topic>Disseminated infection</topic><topic>DNA</topic><topic>Geminiviridae</topic><topic>Gene expression</topic><topic>genome</topic><topic>Genomes</topic><topic>Hypersensitive response</topic><topic>Iran</topic><topic>Leaves</topic><topic>Medical Microbiology</topic><topic>Nicotiana</topic><topic>Nicotiana benthamiana</topic><topic>Original Paper</topic><topic>Pathogenicity</topic><topic>phenotype</topic><topic>Phenotypes</topic><topic>Plant Diseases</topic><topic>Plant Sciences</topic><topic>Plant virus diseases</topic><topic>Plants</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>Tobacco</topic><topic>Tobacco rattle virus</topic><topic>Virology</topic><topic>Virulence Factors - genetics</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ebrahimi, Saeideh</creatorcontrib><creatorcontrib>Eini, Omid</creatorcontrib><creatorcontrib>Koolivand, Davoud</creatorcontrib><creatorcontrib>Varrelmann, Mark</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ebrahimi, Saeideh</au><au>Eini, Omid</au><au>Koolivand, Davoud</au><au>Varrelmann, Mark</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Rep and C1 of Beet curly top Iran virus represent pathogenicity factors and induce hypersensitive response in Nicotiana benthamiana plants</atitle><jtitle>Virus genes</jtitle><stitle>Virus Genes</stitle><addtitle>Virus Genes</addtitle><date>2022-12-01</date><risdate>2022</risdate><volume>58</volume><issue>6</issue><spage>550</spage><epage>559</epage><pages>550-559</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>Beet curly top Iran virus
(BCTIV) is a member of the genus
Becurtovirus
(Family
Geminiviridae
) with a circular single-strand DNA genome. BCTIV causes leaf curling and vein swelling symptoms in plants. However, the potential pathogenicity factor/s in BCTIV is/are not known. This study presents characterization of complementary-sense transcripts of BCTIV and the viral factors in directing the pathogenicity and hypersensitive response (HR) in
Nicotiana benthamiana
plants. In both local and systemic infection, splicing of the complementary transcripts of BCTIV was observed. Notably, a small number (8.3%) of transcripts were spliced to produce Rep (C1:C2) transcripts after deletion of 155 nt (position 1892–2046 from BCTIV). Expression of BCTIV genes in
N. benthamiana
using tobacco rattle virus (TRV)-based vector showed that Rep together with C1 are the main pathogenicity factors which cause typical viral leaf curling symptoms. In addition, the V2 caused a mild leaf curling, thickening, and asymmetric leaves, while the V1
,
V3, and C2 had no clear effect on the plant phenotype. Transient expression of individual viral genes showed that both the C1 and Rep trigger a HR response in
N. benthamiana
. The higher expression of HR marker genes, harpin-induced 1 (
Hin1
) and hypersensitivity‐related (
Hsr203JI)
, supported the role of C1 and Rep in HR response in plants. It is concluded that Rep and C1 are the main pathogenicity factors that also trigger HR response in plants.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35960462</pmid><doi>10.1007/s11262-022-01927-3</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9968-0525</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Virus genes, 2022-12, Vol.58 (6), p.550-559 |
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| language | eng |
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| source | MEDLINE; Springer Nature |
| subjects | Amino acids Apoptosis Beet curly top Iran virus Beta vulgaris Biomedical and Life Sciences Biomedicine Cell death Cloning Disseminated infection DNA Geminiviridae Gene expression genome Genomes Hypersensitive response Iran Leaves Medical Microbiology Nicotiana Nicotiana benthamiana Original Paper Pathogenicity phenotype Phenotypes Plant Diseases Plant Sciences Plant virus diseases Plants Plasmids Proteins Tobacco Tobacco rattle virus Virology Virulence Factors - genetics Viruses |
| title | The Rep and C1 of Beet curly top Iran virus represent pathogenicity factors and induce hypersensitive response in Nicotiana benthamiana plants |
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