miR-106b enhances human mesenchymal stem cell differentiation to spermatogonial stem cells under germ cell profile genes involved in TGF-b signaling pathways

Mesenchymal stem cells can be differentiated into tissue-specific cells. MicroRNAs (miRNAs) regulate the translation of mRNAs involved in the growth and development of a variety of cells, including primordial germ cells (PGCs). This study evaluated male germ cell differentiation from human MSCs by m...

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Veröffentlicht in:In vitro cellular & developmental biology. Animal 2022-08, Vol.58 (7), p.539-548
Hauptverfasser: Mahboudi, Sadaf, Parivar, Kazem, Mazaheri, Zohreh, Irani, Shiva
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container_title In vitro cellular & developmental biology. Animal
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creator Mahboudi, Sadaf
Parivar, Kazem
Mazaheri, Zohreh
Irani, Shiva
description Mesenchymal stem cells can be differentiated into tissue-specific cells. MicroRNAs (miRNAs) regulate the translation of mRNAs involved in the growth and development of a variety of cells, including primordial germ cells (PGCs). This study evaluated male germ cell differentiation from human MSCs by miR-106b. The MSCs were obtained from human adipose tissue. The differentiation of MSCs into PGCs was accomplished by transfection of a lentiviral vector expressing miR-106b. MSCs were treated with bone morphogenic factor 4 as a control and also as a putative inducer of PGC differentiation. PGC was differentiated into spermatogonial-like cells by retinoic acid. Moreover, Dazl , Plzf , Stra8 , Gfra , and Thy1 gene expressions were investigated using real-time PCR. Our results showed that Dazl , Plzf , and Stra8 genes that were treated with BMP4 and miR-106b did not show any significant difference, meaning that miR-106b, like BMP4, is able to differentiate PGC cells from MSCs. In spermatogonial-like cells, Thy1 was significantly unregulated in both the miR-106b and BMP4 groups. Our findings showed that miR-106b regulates the differentiation of MSCs into PGCs. miR-106b influences on the expression of Dazl , Plzf , and Stra8 genes in PGC and Gfra , Stra8 , and Thy1 genes.
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MicroRNAs (miRNAs) regulate the translation of mRNAs involved in the growth and development of a variety of cells, including primordial germ cells (PGCs). This study evaluated male germ cell differentiation from human MSCs by miR-106b. The MSCs were obtained from human adipose tissue. The differentiation of MSCs into PGCs was accomplished by transfection of a lentiviral vector expressing miR-106b. MSCs were treated with bone morphogenic factor 4 as a control and also as a putative inducer of PGC differentiation. PGC was differentiated into spermatogonial-like cells by retinoic acid. Moreover, Dazl , Plzf , Stra8 , Gfra , and Thy1 gene expressions were investigated using real-time PCR. Our results showed that Dazl , Plzf , and Stra8 genes that were treated with BMP4 and miR-106b did not show any significant difference, meaning that miR-106b, like BMP4, is able to differentiate PGC cells from MSCs. 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subjects Adipose tissue
Animal Genetics and Genomics
Biomedical and Life Sciences
Bone morphogenetic protein 4
Cell Biology
Cell Culture
Cell differentiation
Developmental Biology
Differentiation (biology)
Genes
Germ cells
Life Sciences
Mesenchymal stem cells
miRNA
Retinoic acid
Stem Cells
Transfection
title miR-106b enhances human mesenchymal stem cell differentiation to spermatogonial stem cells under germ cell profile genes involved in TGF-b signaling pathways
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