Use of an alternating current amplifier when recording the ERG c-wave to evaluate the function of retinal pigment epithelial cells in rats
Purpose We studied the conditions under which c-waves of the electroretinogram (ERG), that represent retinal pigment epithelium (RPE) function, were detectable using an alternating current (AC) amplifier and whether the c-wave recorded using an AC amplifier was useful for evaluating RPE function. Me...
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| Veröffentlicht in: | Documenta ophthalmologica 2022-10, Vol.145 (2), p.147-155 |
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| container_title | Documenta ophthalmologica |
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| creator | Umeya, Naohisa Miyawaki, Izuru Inada, Hiroshi |
| description | Purpose
We studied the conditions under which c-waves of the electroretinogram (ERG), that represent retinal pigment epithelium (RPE) function, were detectable using an alternating current (AC) amplifier and whether the c-wave recorded using an AC amplifier was useful for evaluating RPE function.
Methods
We recorded ERG responses in rats to 5 s stimuli under the conditions in which the low-cut frequency and the stimulus luminance were varied. In addition, changes in ERGs were studied after intravenous injection of sodium iodate (SI) to induce RPE degeneration.
Results
The c-wave was detected clearly when the frequency of the low-cut filter was set at 0.01 Hz and light stimulus luminances were ≥ − 1.0 log cd/m
2
. The c-wave was attenuated earlier than other waves (e.g., a-wave and b-wave) after SI administration.
Conclusions
The c-wave was easily detectable using an AC amplifier with the low-cut filter set at 0.01 Hz. Using the AC amplifier may allow easier c-wave recording, compared with the conventional use of a direct current (DC) amplifier, and could be useful for evaluating RPE function. |
| doi_str_mv | 10.1007/s10633-022-09890-y |
| format | Article |
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We studied the conditions under which c-waves of the electroretinogram (ERG), that represent retinal pigment epithelium (RPE) function, were detectable using an alternating current (AC) amplifier and whether the c-wave recorded using an AC amplifier was useful for evaluating RPE function.
Methods
We recorded ERG responses in rats to 5 s stimuli under the conditions in which the low-cut frequency and the stimulus luminance were varied. In addition, changes in ERGs were studied after intravenous injection of sodium iodate (SI) to induce RPE degeneration.
Results
The c-wave was detected clearly when the frequency of the low-cut filter was set at 0.01 Hz and light stimulus luminances were ≥ − 1.0 log cd/m
2
. The c-wave was attenuated earlier than other waves (e.g., a-wave and b-wave) after SI administration.
Conclusions
The c-wave was easily detectable using an AC amplifier with the low-cut filter set at 0.01 Hz. Using the AC amplifier may allow easier c-wave recording, compared with the conventional use of a direct current (DC) amplifier, and could be useful for evaluating RPE function.</description><identifier>ISSN: 0012-4486</identifier><identifier>EISSN: 1573-2622</identifier><identifier>DOI: 10.1007/s10633-022-09890-y</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Degeneration ; Electrodes ; Electroretinograms ; Epithelium ; Intravenous administration ; Light ; Medicine ; Medicine & Public Health ; Ophthalmology ; Original Research Article ; Photoreceptors ; Retina ; Retinal pigment epithelium</subject><ispartof>Documenta ophthalmologica, 2022-10, Vol.145 (2), p.147-155</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-8a7bf4619deb3270dc5e8044835c57cf85e7b8042c3bcbf79b411c8b15edc3c3</citedby><cites>FETCH-LOGICAL-c352t-8a7bf4619deb3270dc5e8044835c57cf85e7b8042c3bcbf79b411c8b15edc3c3</cites><orcidid>0000-0002-9351-3520</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10633-022-09890-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10633-022-09890-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Umeya, Naohisa</creatorcontrib><creatorcontrib>Miyawaki, Izuru</creatorcontrib><creatorcontrib>Inada, Hiroshi</creatorcontrib><title>Use of an alternating current amplifier when recording the ERG c-wave to evaluate the function of retinal pigment epithelial cells in rats</title><title>Documenta ophthalmologica</title><addtitle>Doc Ophthalmol</addtitle><description>Purpose
We studied the conditions under which c-waves of the electroretinogram (ERG), that represent retinal pigment epithelium (RPE) function, were detectable using an alternating current (AC) amplifier and whether the c-wave recorded using an AC amplifier was useful for evaluating RPE function.
Methods
We recorded ERG responses in rats to 5 s stimuli under the conditions in which the low-cut frequency and the stimulus luminance were varied. In addition, changes in ERGs were studied after intravenous injection of sodium iodate (SI) to induce RPE degeneration.
Results
The c-wave was detected clearly when the frequency of the low-cut filter was set at 0.01 Hz and light stimulus luminances were ≥ − 1.0 log cd/m
2
. The c-wave was attenuated earlier than other waves (e.g., a-wave and b-wave) after SI administration.
Conclusions
The c-wave was easily detectable using an AC amplifier with the low-cut filter set at 0.01 Hz. Using the AC amplifier may allow easier c-wave recording, compared with the conventional use of a direct current (DC) amplifier, and could be useful for evaluating RPE function.</description><subject>Degeneration</subject><subject>Electrodes</subject><subject>Electroretinograms</subject><subject>Epithelium</subject><subject>Intravenous administration</subject><subject>Light</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Ophthalmology</subject><subject>Original Research Article</subject><subject>Photoreceptors</subject><subject>Retina</subject><subject>Retinal pigment epithelium</subject><issn>0012-4486</issn><issn>1573-2622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU9rHCEYhyU0kE2aL5CTkEsvpv4ZR-cYQpoGAoWSnsVx39l1cZ2JOgn7Ffqp62QLhRx6El6f34OvP4SuGL1hlKqvmdFWCEI5J7TTHSWHE7RiUgnCW84_oRWljJOm0e0ZOs95RyntFNMr9PtXBjwO2EZsQ4EUbfFxg92cEsSC7X4KfvCQ8NsWIk7gxrRegLIFfP_zATvyZl8BlxHDqw2zLfB-NczRFT_GRZ2gKm3Ak9_sFydMviLB15GDEDL2VWxL_oxOBxsyXP49L9Dzt_vnu-_k6cfD493tE3FC8kK0Vf3QtKxbQy-4omsnQdO6mpBOKjdoCaqvA-5E7_pBdX3DmNM9k7B2wokL9OWondL4MkMuZu_z8hAbYZyz4W0nuVZUiIpef0B341y_KFRKMaFlZZpK8SPl0phzgsFMye9tOhhGzdKOObZjajvmvR1zqCFxDOUKxw2kf-r_pP4AOgSU1w</recordid><startdate>20221001</startdate><enddate>20221001</enddate><creator>Umeya, Naohisa</creator><creator>Miyawaki, Izuru</creator><creator>Inada, Hiroshi</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9351-3520</orcidid></search><sort><creationdate>20221001</creationdate><title>Use of an alternating current amplifier when recording the ERG c-wave to evaluate the function of retinal pigment epithelial cells in rats</title><author>Umeya, Naohisa ; Miyawaki, Izuru ; Inada, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-8a7bf4619deb3270dc5e8044835c57cf85e7b8042c3bcbf79b411c8b15edc3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Degeneration</topic><topic>Electrodes</topic><topic>Electroretinograms</topic><topic>Epithelium</topic><topic>Intravenous administration</topic><topic>Light</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Ophthalmology</topic><topic>Original Research Article</topic><topic>Photoreceptors</topic><topic>Retina</topic><topic>Retinal pigment epithelium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Umeya, Naohisa</creatorcontrib><creatorcontrib>Miyawaki, Izuru</creatorcontrib><creatorcontrib>Inada, Hiroshi</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Documenta ophthalmologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Umeya, Naohisa</au><au>Miyawaki, Izuru</au><au>Inada, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of an alternating current amplifier when recording the ERG c-wave to evaluate the function of retinal pigment epithelial cells in rats</atitle><jtitle>Documenta ophthalmologica</jtitle><stitle>Doc Ophthalmol</stitle><date>2022-10-01</date><risdate>2022</risdate><volume>145</volume><issue>2</issue><spage>147</spage><epage>155</epage><pages>147-155</pages><issn>0012-4486</issn><eissn>1573-2622</eissn><abstract>Purpose
We studied the conditions under which c-waves of the electroretinogram (ERG), that represent retinal pigment epithelium (RPE) function, were detectable using an alternating current (AC) amplifier and whether the c-wave recorded using an AC amplifier was useful for evaluating RPE function.
Methods
We recorded ERG responses in rats to 5 s stimuli under the conditions in which the low-cut frequency and the stimulus luminance were varied. In addition, changes in ERGs were studied after intravenous injection of sodium iodate (SI) to induce RPE degeneration.
Results
The c-wave was detected clearly when the frequency of the low-cut filter was set at 0.01 Hz and light stimulus luminances were ≥ − 1.0 log cd/m
2
. The c-wave was attenuated earlier than other waves (e.g., a-wave and b-wave) after SI administration.
Conclusions
The c-wave was easily detectable using an AC amplifier with the low-cut filter set at 0.01 Hz. Using the AC amplifier may allow easier c-wave recording, compared with the conventional use of a direct current (DC) amplifier, and could be useful for evaluating RPE function.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s10633-022-09890-y</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9351-3520</orcidid></addata></record> |
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| subjects | Degeneration Electrodes Electroretinograms Epithelium Intravenous administration Light Medicine Medicine & Public Health Ophthalmology Original Research Article Photoreceptors Retina Retinal pigment epithelium |
| title | Use of an alternating current amplifier when recording the ERG c-wave to evaluate the function of retinal pigment epithelial cells in rats |
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