Fusion between Glioma Stem Cells and Mesenchymal Stem Cells Promotes Malignant Progression in 3D-Bioprinted Models
The interaction between glioma stem cells (GSCs) and mesenchymal stem cells (MSCs) in the glioma microenvironment is considered to be an important factor in promoting tumor progression, but the mechanism is still not fully elucidated. To further elucidate the interaction between GSCs and MSCs, two 3...
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| Veröffentlicht in: | ACS applied materials & interfaces 2022-08, Vol.14 (31), p.35344-35356 |
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| container_title | ACS applied materials & interfaces |
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| creator | Dai, Xingliang Shao, Yuxuan Tian, Xuefeng Cao, Xiaoyan Ye, Lei Gao, Peng Cheng, Hongwei Wang, Xianwen |
| description | The interaction between glioma stem cells (GSCs) and mesenchymal stem cells (MSCs) in the glioma microenvironment is considered to be an important factor in promoting tumor progression, but the mechanism is still not fully elucidated. To further elucidate the interaction between GSCs and MSCs, two 3D-bioprinted tumor models (low-temperature molding and coaxial bioprinting) were used to simulate the tumor growth microenvironment. Cell fusion between GSCs and MSCs was found by the method of Cre-LoxP switch gene and RFP/GFP dual-color fluorescence tracing. The fused cells coexpressed biomarkers of GSCs and MSCs, showing stronger proliferation, cloning, and invasion abilities than GSCs and MSCs. In addition, the fused cells have stronger tumorigenic properties in nude mice, showing the pathological features of malignant tumors. In conclusion, GSCs and MSCs undergo cell fusion in 3D-bioprinted models, and the fused cells have a higher degree of malignancy than parental cells, which promotes the progression of glioma. |
| doi_str_mv | 10.1021/acsami.2c06658 |
| format | Article |
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To further elucidate the interaction between GSCs and MSCs, two 3D-bioprinted tumor models (low-temperature molding and coaxial bioprinting) were used to simulate the tumor growth microenvironment. Cell fusion between GSCs and MSCs was found by the method of Cre-LoxP switch gene and RFP/GFP dual-color fluorescence tracing. The fused cells coexpressed biomarkers of GSCs and MSCs, showing stronger proliferation, cloning, and invasion abilities than GSCs and MSCs. In addition, the fused cells have stronger tumorigenic properties in nude mice, showing the pathological features of malignant tumors. In conclusion, GSCs and MSCs undergo cell fusion in 3D-bioprinted models, and the fused cells have a higher degree of malignancy than parental cells, which promotes the progression of glioma.</description><identifier>ISSN: 1944-8244</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/acsami.2c06658</identifier><language>eng</language><publisher>American Chemical Society</publisher><subject>Biological and Medical Applications of Materials and Interfaces</subject><ispartof>ACS applied materials & interfaces, 2022-08, Vol.14 (31), p.35344-35356</ispartof><rights>2022 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a307t-6fee185317d11db45180782838f1b6fab6d21f0bd64cddbbc8a7607259294a43</citedby><cites>FETCH-LOGICAL-a307t-6fee185317d11db45180782838f1b6fab6d21f0bd64cddbbc8a7607259294a43</cites><orcidid>0000-0002-6343-440X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsami.2c06658$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsami.2c06658$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids></links><search><creatorcontrib>Dai, Xingliang</creatorcontrib><creatorcontrib>Shao, Yuxuan</creatorcontrib><creatorcontrib>Tian, Xuefeng</creatorcontrib><creatorcontrib>Cao, Xiaoyan</creatorcontrib><creatorcontrib>Ye, Lei</creatorcontrib><creatorcontrib>Gao, Peng</creatorcontrib><creatorcontrib>Cheng, Hongwei</creatorcontrib><creatorcontrib>Wang, Xianwen</creatorcontrib><title>Fusion between Glioma Stem Cells and Mesenchymal Stem Cells Promotes Malignant Progression in 3D-Bioprinted Models</title><title>ACS applied materials & interfaces</title><addtitle>ACS Appl. 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In conclusion, GSCs and MSCs undergo cell fusion in 3D-bioprinted models, and the fused cells have a higher degree of malignancy than parental cells, which promotes the progression of glioma.</description><subject>Biological and Medical Applications of Materials and Interfaces</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1UEFLwzAYDaLgnF495yhCZ5K2aXrU6aawoeDuIW2-zow2mUmH7N-b2SFePH0f73vv8b6H0DUlE0oYvVN1UJ2ZsJpwnosTNKJlliWC5ez0d8-yc3QRwoYQnjKSj5Cf7YJxFlfQfwFYPG-N6xR-76HDU2jbgJXVeAkBbP2x71T79_TmXed6CHipWrO2yvYHaO0h_Hgai9PH5MG4rTe2h2jjNLThEp01qg1wdZxjtJo9rabPyeJ1_jK9XyQqJUWf8AaAijylhaZUV1lOBSkEE6loaMUbVXHNaEMqzbNa66qqhSo4KVhesjJTWTpGN4Pt1rvPHYRedibUMbey4HZBMl7m0a0seKROBmrtXQgeGhkTd8rvJSXy0K0cupXHbqPgdhBEXG7cztv4yH_kb5IOfLk</recordid><startdate>20220810</startdate><enddate>20220810</enddate><creator>Dai, Xingliang</creator><creator>Shao, Yuxuan</creator><creator>Tian, Xuefeng</creator><creator>Cao, Xiaoyan</creator><creator>Ye, Lei</creator><creator>Gao, Peng</creator><creator>Cheng, Hongwei</creator><creator>Wang, Xianwen</creator><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6343-440X</orcidid></search><sort><creationdate>20220810</creationdate><title>Fusion between Glioma Stem Cells and Mesenchymal Stem Cells Promotes Malignant Progression in 3D-Bioprinted Models</title><author>Dai, Xingliang ; Shao, Yuxuan ; Tian, Xuefeng ; Cao, Xiaoyan ; Ye, Lei ; Gao, Peng ; Cheng, Hongwei ; Wang, Xianwen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a307t-6fee185317d11db45180782838f1b6fab6d21f0bd64cddbbc8a7607259294a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biological and Medical Applications of Materials and Interfaces</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dai, Xingliang</creatorcontrib><creatorcontrib>Shao, Yuxuan</creatorcontrib><creatorcontrib>Tian, Xuefeng</creatorcontrib><creatorcontrib>Cao, Xiaoyan</creatorcontrib><creatorcontrib>Ye, Lei</creatorcontrib><creatorcontrib>Gao, Peng</creatorcontrib><creatorcontrib>Cheng, Hongwei</creatorcontrib><creatorcontrib>Wang, Xianwen</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dai, Xingliang</au><au>Shao, Yuxuan</au><au>Tian, Xuefeng</au><au>Cao, Xiaoyan</au><au>Ye, Lei</au><au>Gao, Peng</au><au>Cheng, Hongwei</au><au>Wang, Xianwen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fusion between Glioma Stem Cells and Mesenchymal Stem Cells Promotes Malignant Progression in 3D-Bioprinted Models</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2022-08-10</date><risdate>2022</risdate><volume>14</volume><issue>31</issue><spage>35344</spage><epage>35356</epage><pages>35344-35356</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>The interaction between glioma stem cells (GSCs) and mesenchymal stem cells (MSCs) in the glioma microenvironment is considered to be an important factor in promoting tumor progression, but the mechanism is still not fully elucidated. To further elucidate the interaction between GSCs and MSCs, two 3D-bioprinted tumor models (low-temperature molding and coaxial bioprinting) were used to simulate the tumor growth microenvironment. Cell fusion between GSCs and MSCs was found by the method of Cre-LoxP switch gene and RFP/GFP dual-color fluorescence tracing. The fused cells coexpressed biomarkers of GSCs and MSCs, showing stronger proliferation, cloning, and invasion abilities than GSCs and MSCs. In addition, the fused cells have stronger tumorigenic properties in nude mice, showing the pathological features of malignant tumors. In conclusion, GSCs and MSCs undergo cell fusion in 3D-bioprinted models, and the fused cells have a higher degree of malignancy than parental cells, which promotes the progression of glioma.</abstract><pub>American Chemical Society</pub><doi>10.1021/acsami.2c06658</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-6343-440X</orcidid></addata></record> |
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| title | Fusion between Glioma Stem Cells and Mesenchymal Stem Cells Promotes Malignant Progression in 3D-Bioprinted Models |
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