In vitro evaluation of antiviral activity of Shouchella clausii probiotic strain and bacterial supernatant against herpes simplex virus type 1
Herpes simplex virus-1 (HSV-1) is an important human neurotropic virus infecting 70% of the world population. Due to the emergence of viral resistance via mutations in HSV-1 genes and some of the adverse effects of antiviral compounds, there is a growing need for safe, novel, and effective therapeut...
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Veröffentlicht in: | Archives of microbiology 2022-08, Vol.204 (8), p.522-522, Article 522 |
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description | Herpes simplex virus-1 (HSV-1) is an important human neurotropic virus infecting 70% of the world population. Due to the emergence of viral resistance via mutations in HSV-1 genes and some of the adverse effects of antiviral compounds, there is a growing need for safe, novel, and effective therapeutic and preventive strategies. The aim of the present study was to investigate for the first time the potential antiviral activity of
Shouchella clausii
probiotic strain and bacterial supernatant against HSV-1. The MTT assay was used to determine the possible cytotoxicity of the
S. clausii
and bacterial supernatant. Vero cells were treated by
S. clausii
, bacterial supernatant, and HSV-1 under pre-treatment (incubation of Vero cells with
S. clausii
then HSV-1 inoculation), pre-incubation (mixture of co-incubated HSV-1/
S. clausii
added to Vero cell), competition (adding HSV-1 and
S. clausii
into Vero cells simultaneously) and post-treatment (Vero cells inoculated with HSV-1 then incubated with
S. clausii
) assays. Viral titer reduction (TCID
50
) and viral DNA relative quantification by real-time PCR were measured in each experimental condition. The results indicated that
S. clausii
and its supernatant had the greatest inhibitory activity toward HSV-1 in pre-treatment assay. The HSV-1 titer treated with
S. clausii
, and bacterial supernatant was 3.6 and 2.2 Log
10
TCID
50
/mL lower compared to the control (7.66 Log
10
TCID
50
/mL). Results showed an antiviral effect of
S. clausii
and its supernatant.
S. clausii
could be considered as a novel inhibitor for HSV-1 infection. |
doi_str_mv | 10.1007/s00203-022-03137-9 |
format | Article |
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Shouchella clausii
probiotic strain and bacterial supernatant against HSV-1. The MTT assay was used to determine the possible cytotoxicity of the
S. clausii
and bacterial supernatant. Vero cells were treated by
S. clausii
, bacterial supernatant, and HSV-1 under pre-treatment (incubation of Vero cells with
S. clausii
then HSV-1 inoculation), pre-incubation (mixture of co-incubated HSV-1/
S. clausii
added to Vero cell), competition (adding HSV-1 and
S. clausii
into Vero cells simultaneously) and post-treatment (Vero cells inoculated with HSV-1 then incubated with
S. clausii
) assays. Viral titer reduction (TCID
50
) and viral DNA relative quantification by real-time PCR were measured in each experimental condition. The results indicated that
S. clausii
and its supernatant had the greatest inhibitory activity toward HSV-1 in pre-treatment assay. The HSV-1 titer treated with
S. clausii
, and bacterial supernatant was 3.6 and 2.2 Log
10
TCID
50
/mL lower compared to the control (7.66 Log
10
TCID
50
/mL). Results showed an antiviral effect of
S. clausii
and its supernatant.
S. clausii
could be considered as a novel inhibitor for HSV-1 infection.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-022-03137-9</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antiviral activity ; Assaying ; Bacteria ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Cytotoxicity ; Ecology ; Herpes simplex ; Herpes viruses ; Incubation ; Inoculation ; Life Sciences ; Microbial Ecology ; Microbiology ; Mutation ; Original Paper ; Pretreatment ; Probiotics ; Time measurement ; Toxicity ; Vero cells ; Viruses ; World population</subject><ispartof>Archives of microbiology, 2022-08, Vol.204 (8), p.522-522, Article 522</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-b9439c4b897d05ae297699545c26dad19e745bac0c1b879570de46ef28323ecd3</citedby><cites>FETCH-LOGICAL-c282t-b9439c4b897d05ae297699545c26dad19e745bac0c1b879570de46ef28323ecd3</cites><orcidid>0000-0003-2741-2745</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-022-03137-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-022-03137-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>barzoki, Mehdi Gholami</creatorcontrib><creatorcontrib>Malekshahi, Somayeh Shatizadeh</creatorcontrib><creatorcontrib>Shayestehpour, Mohammad</creatorcontrib><title>In vitro evaluation of antiviral activity of Shouchella clausii probiotic strain and bacterial supernatant against herpes simplex virus type 1</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><description>Herpes simplex virus-1 (HSV-1) is an important human neurotropic virus infecting 70% of the world population. Due to the emergence of viral resistance via mutations in HSV-1 genes and some of the adverse effects of antiviral compounds, there is a growing need for safe, novel, and effective therapeutic and preventive strategies. The aim of the present study was to investigate for the first time the potential antiviral activity of
Shouchella clausii
probiotic strain and bacterial supernatant against HSV-1. The MTT assay was used to determine the possible cytotoxicity of the
S. clausii
and bacterial supernatant. Vero cells were treated by
S. clausii
, bacterial supernatant, and HSV-1 under pre-treatment (incubation of Vero cells with
S. clausii
then HSV-1 inoculation), pre-incubation (mixture of co-incubated HSV-1/
S. clausii
added to Vero cell), competition (adding HSV-1 and
S. clausii
into Vero cells simultaneously) and post-treatment (Vero cells inoculated with HSV-1 then incubated with
S. clausii
) assays. Viral titer reduction (TCID
50
) and viral DNA relative quantification by real-time PCR were measured in each experimental condition. The results indicated that
S. clausii
and its supernatant had the greatest inhibitory activity toward HSV-1 in pre-treatment assay. The HSV-1 titer treated with
S. clausii
, and bacterial supernatant was 3.6 and 2.2 Log
10
TCID
50
/mL lower compared to the control (7.66 Log
10
TCID
50
/mL). Results showed an antiviral effect of
S. clausii
and its supernatant.
S. clausii
could be considered as a novel inhibitor for HSV-1 infection.</description><subject>Antiviral activity</subject><subject>Assaying</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Cytotoxicity</subject><subject>Ecology</subject><subject>Herpes simplex</subject><subject>Herpes viruses</subject><subject>Incubation</subject><subject>Inoculation</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Mutation</subject><subject>Original Paper</subject><subject>Pretreatment</subject><subject>Probiotics</subject><subject>Time measurement</subject><subject>Toxicity</subject><subject>Vero cells</subject><subject>Viruses</subject><subject>World population</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc-KFDEQh4O44LjuC3gKePHSWkm6J8lRFl0XFjyosLeQTtfsZOnptKn04LyEz7wZWxA8eMq_76tU8WPstYB3AkC_JwAJqgEpG1BC6cY-YxvRqnrU8v4524AC2Rir1Av2kugRQEhjzIb9up34MZacOB79uPgS08TTjvupxGPMfuQ-nHfldL79uk9L2OM4eh5Gv1CMfM6pj6nEwKlkH6dqDryvEuZYbVpmzJMvtR73D_WdCt9jnpE4xcM84s_6fV6Il9OMXLxiFzs_El79WS_Z908fv11_bu6-3Nxef7hrgjSyNL1tlQ1tb6weoPMord5a27VdkNvBD8KibrvaBATRG207DQO2W9xJo6TCMKhL9natW9v_sSAVd4gUzoNNmBZycmtb21kldEXf_IM-pqWONK4UWGONqpRcqZATUcadm3M8-HxyAtw5IrdG5GpE7ndEzlZJrRJVeHrA_Lf0f6wnxVCWyA</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>barzoki, Mehdi Gholami</creator><creator>Malekshahi, Somayeh Shatizadeh</creator><creator>Shayestehpour, Mohammad</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2741-2745</orcidid></search><sort><creationdate>20220801</creationdate><title>In vitro evaluation of antiviral activity of Shouchella clausii probiotic strain and bacterial supernatant against herpes simplex virus type 1</title><author>barzoki, Mehdi Gholami ; Malekshahi, Somayeh Shatizadeh ; Shayestehpour, Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-b9439c4b897d05ae297699545c26dad19e745bac0c1b879570de46ef28323ecd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antiviral activity</topic><topic>Assaying</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Cytotoxicity</topic><topic>Ecology</topic><topic>Herpes simplex</topic><topic>Herpes viruses</topic><topic>Incubation</topic><topic>Inoculation</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Mutation</topic><topic>Original Paper</topic><topic>Pretreatment</topic><topic>Probiotics</topic><topic>Time measurement</topic><topic>Toxicity</topic><topic>Vero cells</topic><topic>Viruses</topic><topic>World population</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>barzoki, Mehdi Gholami</creatorcontrib><creatorcontrib>Malekshahi, Somayeh Shatizadeh</creatorcontrib><creatorcontrib>Shayestehpour, Mohammad</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>barzoki, Mehdi Gholami</au><au>Malekshahi, Somayeh Shatizadeh</au><au>Shayestehpour, Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro evaluation of antiviral activity of Shouchella clausii probiotic strain and bacterial supernatant against herpes simplex virus type 1</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><date>2022-08-01</date><risdate>2022</risdate><volume>204</volume><issue>8</issue><spage>522</spage><epage>522</epage><pages>522-522</pages><artnum>522</artnum><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Herpes simplex virus-1 (HSV-1) is an important human neurotropic virus infecting 70% of the world population. Due to the emergence of viral resistance via mutations in HSV-1 genes and some of the adverse effects of antiviral compounds, there is a growing need for safe, novel, and effective therapeutic and preventive strategies. The aim of the present study was to investigate for the first time the potential antiviral activity of
Shouchella clausii
probiotic strain and bacterial supernatant against HSV-1. The MTT assay was used to determine the possible cytotoxicity of the
S. clausii
and bacterial supernatant. Vero cells were treated by
S. clausii
, bacterial supernatant, and HSV-1 under pre-treatment (incubation of Vero cells with
S. clausii
then HSV-1 inoculation), pre-incubation (mixture of co-incubated HSV-1/
S. clausii
added to Vero cell), competition (adding HSV-1 and
S. clausii
into Vero cells simultaneously) and post-treatment (Vero cells inoculated with HSV-1 then incubated with
S. clausii
) assays. Viral titer reduction (TCID
50
) and viral DNA relative quantification by real-time PCR were measured in each experimental condition. The results indicated that
S. clausii
and its supernatant had the greatest inhibitory activity toward HSV-1 in pre-treatment assay. The HSV-1 titer treated with
S. clausii
, and bacterial supernatant was 3.6 and 2.2 Log
10
TCID
50
/mL lower compared to the control (7.66 Log
10
TCID
50
/mL). Results showed an antiviral effect of
S. clausii
and its supernatant.
S. clausii
could be considered as a novel inhibitor for HSV-1 infection.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00203-022-03137-9</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-2741-2745</orcidid></addata></record> |
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language | eng |
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source | SpringerLink Journals - AutoHoldings |
subjects | Antiviral activity Assaying Bacteria Biochemistry Biomedical and Life Sciences Biotechnology Cell Biology Cytotoxicity Ecology Herpes simplex Herpes viruses Incubation Inoculation Life Sciences Microbial Ecology Microbiology Mutation Original Paper Pretreatment Probiotics Time measurement Toxicity Vero cells Viruses World population |
title | In vitro evaluation of antiviral activity of Shouchella clausii probiotic strain and bacterial supernatant against herpes simplex virus type 1 |
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