Endometrial glycogen metabolism during early pregnancy in mice
Glucose is critical during early pregnancy. The uterus can store glucose as glycogen but uterine glycogen metabolism is poorly understood. This study analyzed glycogen storage and localization of glycogen metabolizing enzymes from proestrus until implantation in the murine uterus. Quantification of...
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| Veröffentlicht in: | Molecular reproduction and development 2022-09, Vol.89 (9), p.431-440 |
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| creator | Chen, Ziting Sandoval, Kassandra Dean, Matthew |
| description | Glucose is critical during early pregnancy. The uterus can store glucose as glycogen but uterine glycogen metabolism is poorly understood. This study analyzed glycogen storage and localization of glycogen metabolizing enzymes from proestrus until implantation in the murine uterus. Quantification of diastase‐labile periodic acid–Schiff (PAS) staining showed glycogen in the glandular epithelium decreased 71.4% at 1.5 days postcoitum (DPC) and 62.13% at DPC 3.5 compared to proestrus. In the luminal epithelium, glycogen was the highest at proestrus, decreased 46.2% at DPC 1.5 and 63.2% at DPC 3.5. Immunostaining showed that before implantation, glycogen metabolizing enzymes were primarily localized to the glandular and luminal epithelium. Stromal glycogen was low from proestrus to DPC 3.5. However, at the DPC 5.5 implantation sites, stromal glycogen levels increased sevenfold. Similarly, artificial decidualization resulted in a fivefold increase in glycogen levels. In both models, decidualization increased expression of glycogen synthase as determine by immunohistochemistry and western blot. In conclusion, glycogen levels decreased in the uterine epithelium before implantation, indicating that it could be used to support preimplantation embryos. Decidualization resulted in a dramatic increase in stromal glycogen levels, suggesting it may have an important, but yet undefined, role in pregnancy. |
| doi_str_mv | 10.1002/mrd.23634 |
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The uterus can store glucose as glycogen but uterine glycogen metabolism is poorly understood. This study analyzed glycogen storage and localization of glycogen metabolizing enzymes from proestrus until implantation in the murine uterus. Quantification of diastase‐labile periodic acid–Schiff (PAS) staining showed glycogen in the glandular epithelium decreased 71.4% at 1.5 days postcoitum (DPC) and 62.13% at DPC 3.5 compared to proestrus. In the luminal epithelium, glycogen was the highest at proestrus, decreased 46.2% at DPC 1.5 and 63.2% at DPC 3.5. Immunostaining showed that before implantation, glycogen metabolizing enzymes were primarily localized to the glandular and luminal epithelium. Stromal glycogen was low from proestrus to DPC 3.5. However, at the DPC 5.5 implantation sites, stromal glycogen levels increased sevenfold. Similarly, artificial decidualization resulted in a fivefold increase in glycogen levels. In both models, decidualization increased expression of glycogen synthase as determine by immunohistochemistry and western blot. In conclusion, glycogen levels decreased in the uterine epithelium before implantation, indicating that it could be used to support preimplantation embryos. Decidualization resulted in a dramatic increase in stromal glycogen levels, suggesting it may have an important, but yet undefined, role in pregnancy.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.23634</identifier><identifier>PMID: 35842832</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Amylases - chemistry ; Animals ; decidualization ; Embryos ; Endometrium ; Endometrium - chemistry ; Endometrium - metabolism ; Enzymes ; Epithelium ; Estrus cycle ; Female ; glucose ; Glucose - metabolism ; glucose‐6‐phosphatase ; Glycogen ; Glycogen - analysis ; Glycogen - metabolism ; glycogen phosphorylase ; Glycogen synthase ; Glycogen Synthase - metabolism ; hexokinase ; Immunohistochemistry ; Implantation ; Localization ; Metabolism ; Mice ; Periodic Acid-Schiff Reaction ; Pregnancy ; Pregnancy Trimester, First - metabolism ; Uterus</subject><ispartof>Molecular reproduction and development, 2022-09, Vol.89 (9), p.431-440</ispartof><rights>2022 The Authors. published by Wiley Periodicals LLC.</rights><rights>2022 The Authors. Molecular Reproduction and Development published by Wiley Periodicals LLC.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3184-644590efd58e6d6758351a7e176c98252009e002c0c86a43f6e915963797127b3</citedby><cites>FETCH-LOGICAL-c3184-644590efd58e6d6758351a7e176c98252009e002c0c86a43f6e915963797127b3</cites><orcidid>0000-0001-5014-9311</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.23634$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.23634$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35842832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Ziting</creatorcontrib><creatorcontrib>Sandoval, Kassandra</creatorcontrib><creatorcontrib>Dean, Matthew</creatorcontrib><title>Endometrial glycogen metabolism during early pregnancy in mice</title><title>Molecular reproduction and development</title><addtitle>Mol Reprod Dev</addtitle><description>Glucose is critical during early pregnancy. The uterus can store glucose as glycogen but uterine glycogen metabolism is poorly understood. This study analyzed glycogen storage and localization of glycogen metabolizing enzymes from proestrus until implantation in the murine uterus. Quantification of diastase‐labile periodic acid–Schiff (PAS) staining showed glycogen in the glandular epithelium decreased 71.4% at 1.5 days postcoitum (DPC) and 62.13% at DPC 3.5 compared to proestrus. In the luminal epithelium, glycogen was the highest at proestrus, decreased 46.2% at DPC 1.5 and 63.2% at DPC 3.5. Immunostaining showed that before implantation, glycogen metabolizing enzymes were primarily localized to the glandular and luminal epithelium. Stromal glycogen was low from proestrus to DPC 3.5. However, at the DPC 5.5 implantation sites, stromal glycogen levels increased sevenfold. Similarly, artificial decidualization resulted in a fivefold increase in glycogen levels. In both models, decidualization increased expression of glycogen synthase as determine by immunohistochemistry and western blot. In conclusion, glycogen levels decreased in the uterine epithelium before implantation, indicating that it could be used to support preimplantation embryos. Decidualization resulted in a dramatic increase in stromal glycogen levels, suggesting it may have an important, but yet undefined, role in pregnancy.</description><subject>Amylases - chemistry</subject><subject>Animals</subject><subject>decidualization</subject><subject>Embryos</subject><subject>Endometrium</subject><subject>Endometrium - chemistry</subject><subject>Endometrium - metabolism</subject><subject>Enzymes</subject><subject>Epithelium</subject><subject>Estrus cycle</subject><subject>Female</subject><subject>glucose</subject><subject>Glucose - metabolism</subject><subject>glucose‐6‐phosphatase</subject><subject>Glycogen</subject><subject>Glycogen - analysis</subject><subject>Glycogen - metabolism</subject><subject>glycogen phosphorylase</subject><subject>Glycogen synthase</subject><subject>Glycogen Synthase - metabolism</subject><subject>hexokinase</subject><subject>Immunohistochemistry</subject><subject>Implantation</subject><subject>Localization</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Periodic Acid-Schiff Reaction</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, First - metabolism</subject><subject>Uterus</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp10MtKAzEUBuAgiq3VhS8gA250MTb3STaC1HqBiiAK7kKaOS1T5lKTDjJvb-pUF4KrHMLHzzk_QqcEXxGM6bjy-RVlkvE9NCRYq5RmWuxvZ45TLuj7AB2FsMIYa63wIRowoThVjA7R9bTOmwo2vrBlsiw71yyhTuKHnTdlEaokb31RLxOwvuyStYdlbWvXJUVEhYNjdLCwZYCT3TtCb3fT18lDOnu-f5zczFLHiOKp5FxoDItcKJC5zIRigtgMSCadVlTQuBnESxx2SlrOFhI0EVqyTGeEZnM2Qhd97to3Hy2EjamK4KAsbQ1NGwyVmmCBOdGRnv-hq6b1ddzO0IxIFhmjUV32yvkmBA8Ls_ZFZX1nCDbbUk0s1XyXGu3ZLrGdV5D_yp8WIxj34LMoofs_yTy93PaRX-Kvfc4</recordid><startdate>202209</startdate><enddate>202209</enddate><creator>Chen, Ziting</creator><creator>Sandoval, Kassandra</creator><creator>Dean, Matthew</creator><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5014-9311</orcidid></search><sort><creationdate>202209</creationdate><title>Endometrial glycogen metabolism during early pregnancy in mice</title><author>Chen, Ziting ; Sandoval, Kassandra ; Dean, Matthew</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3184-644590efd58e6d6758351a7e176c98252009e002c0c86a43f6e915963797127b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amylases - chemistry</topic><topic>Animals</topic><topic>decidualization</topic><topic>Embryos</topic><topic>Endometrium</topic><topic>Endometrium - chemistry</topic><topic>Endometrium - metabolism</topic><topic>Enzymes</topic><topic>Epithelium</topic><topic>Estrus cycle</topic><topic>Female</topic><topic>glucose</topic><topic>Glucose - metabolism</topic><topic>glucose‐6‐phosphatase</topic><topic>Glycogen</topic><topic>Glycogen - analysis</topic><topic>Glycogen - metabolism</topic><topic>glycogen phosphorylase</topic><topic>Glycogen synthase</topic><topic>Glycogen Synthase - metabolism</topic><topic>hexokinase</topic><topic>Immunohistochemistry</topic><topic>Implantation</topic><topic>Localization</topic><topic>Metabolism</topic><topic>Mice</topic><topic>Periodic Acid-Schiff Reaction</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, First - metabolism</topic><topic>Uterus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Ziting</creatorcontrib><creatorcontrib>Sandoval, Kassandra</creatorcontrib><creatorcontrib>Dean, Matthew</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Ziting</au><au>Sandoval, Kassandra</au><au>Dean, Matthew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endometrial glycogen metabolism during early pregnancy in mice</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol Reprod Dev</addtitle><date>2022-09</date><risdate>2022</risdate><volume>89</volume><issue>9</issue><spage>431</spage><epage>440</epage><pages>431-440</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><abstract>Glucose is critical during early pregnancy. The uterus can store glucose as glycogen but uterine glycogen metabolism is poorly understood. This study analyzed glycogen storage and localization of glycogen metabolizing enzymes from proestrus until implantation in the murine uterus. Quantification of diastase‐labile periodic acid–Schiff (PAS) staining showed glycogen in the glandular epithelium decreased 71.4% at 1.5 days postcoitum (DPC) and 62.13% at DPC 3.5 compared to proestrus. In the luminal epithelium, glycogen was the highest at proestrus, decreased 46.2% at DPC 1.5 and 63.2% at DPC 3.5. Immunostaining showed that before implantation, glycogen metabolizing enzymes were primarily localized to the glandular and luminal epithelium. Stromal glycogen was low from proestrus to DPC 3.5. However, at the DPC 5.5 implantation sites, stromal glycogen levels increased sevenfold. Similarly, artificial decidualization resulted in a fivefold increase in glycogen levels. In both models, decidualization increased expression of glycogen synthase as determine by immunohistochemistry and western blot. In conclusion, glycogen levels decreased in the uterine epithelium before implantation, indicating that it could be used to support preimplantation embryos. Decidualization resulted in a dramatic increase in stromal glycogen levels, suggesting it may have an important, but yet undefined, role in pregnancy.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35842832</pmid><doi>10.1002/mrd.23634</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5014-9311</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amylases - chemistry Animals decidualization Embryos Endometrium Endometrium - chemistry Endometrium - metabolism Enzymes Epithelium Estrus cycle Female glucose Glucose - metabolism glucose‐6‐phosphatase Glycogen Glycogen - analysis Glycogen - metabolism glycogen phosphorylase Glycogen synthase Glycogen Synthase - metabolism hexokinase Immunohistochemistry Implantation Localization Metabolism Mice Periodic Acid-Schiff Reaction Pregnancy Pregnancy Trimester, First - metabolism Uterus |
| title | Endometrial glycogen metabolism during early pregnancy in mice |
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