Phytochemical profile and protective effects on myocardial ischaemia-reperfusion injury of sweated and non-sweated Salvia miltiorrhiza. Bge alcoholic extracts
Abstract Objectives This study aims to compare the fingerprint and the content of the three components of sweated and non-sweated Salvia miltiorrhiza alcoholic extracts (SSAE and NSAE). It also aims to investigate the difference in protective effects of SSAE and NSAE on myocardial ischaemia-reperfus...
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| Veröffentlicht in: | Journal of pharmacy and pharmacology 2022-09, Vol.74 (9), p.1230-1240 |
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| container_title | Journal of pharmacy and pharmacology |
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| creator | Shan, Xiaoxiao Xiao, Yaoyao Hong, Bangzhen Li, Ling Chen, Yueting Wang, Guokai Yu, Nianjun Peng, Daiyin Zhang, Caiyun Wang, Lei Chen, Weidong |
| description | Abstract
Objectives
This study aims to compare the fingerprint and the content of the three components of sweated and non-sweated Salvia miltiorrhiza alcoholic extracts (SSAE and NSAE). It also aims to investigate the difference in protective effects of SSAE and NSAE on myocardial ischaemia-reperfusion injury (MIRI).
Methods
The fingerprints of SSAE and NSAE were established by HPLC with a UV detector to identify the common peaks and detect the content of the three major components (cryptotanshinone, tanshinone I and tanshinone IIA). The protective effects of SSAE and NSAE were compared with MIRI rat model after orally administered SSAE and NSAE (2 g/kg of raw drug) for 7 days. The ST segment, PR and QT interval changes and the infarct size were assessed in the rat hearts. Moreover, the activity of aspartate transaminase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and the level of cardiac troponin I (cTn I) in serum as well as the cardiac H&E staining were evaluated.
Key findings
The results showed that the fingerprints of SSAE and NSAE were similar, and cluster analysis showed that the sweating methods had effects on the alcoholic extracts. The content determination showed that sweating could increase the total content of cryptotanshinone, tanshinone I and tanshinone IIA of S. miltiorrhiza. The results of electrocardiograms (ECG) showed that SSAE could make the ST segment drop more obviously, PR and QT intervals become shorter, and the size of the infarct much smaller. Compared with NSAE, SSAE had more significant effects on the enzymatic activity of AST, LDH and the level of cTn I in serum. The H&E staining showed that both SSAE and NSAE could reduce the degree of heart damage.
Conclusions
The present investigation results demonstrated that sweating increased the content of tanshinone components in S. miltiorrhiza alcoholic extracts, and SSAE had a better protective effect on MIRI.
Graphical Abstract
Graphical Abstract |
| doi_str_mv | 10.1093/jpp/rgac012 |
| format | Article |
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Objectives
This study aims to compare the fingerprint and the content of the three components of sweated and non-sweated Salvia miltiorrhiza alcoholic extracts (SSAE and NSAE). It also aims to investigate the difference in protective effects of SSAE and NSAE on myocardial ischaemia-reperfusion injury (MIRI).
Methods
The fingerprints of SSAE and NSAE were established by HPLC with a UV detector to identify the common peaks and detect the content of the three major components (cryptotanshinone, tanshinone I and tanshinone IIA). The protective effects of SSAE and NSAE were compared with MIRI rat model after orally administered SSAE and NSAE (2 g/kg of raw drug) for 7 days. The ST segment, PR and QT interval changes and the infarct size were assessed in the rat hearts. Moreover, the activity of aspartate transaminase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and the level of cardiac troponin I (cTn I) in serum as well as the cardiac H&E staining were evaluated.
Key findings
The results showed that the fingerprints of SSAE and NSAE were similar, and cluster analysis showed that the sweating methods had effects on the alcoholic extracts. The content determination showed that sweating could increase the total content of cryptotanshinone, tanshinone I and tanshinone IIA of S. miltiorrhiza. The results of electrocardiograms (ECG) showed that SSAE could make the ST segment drop more obviously, PR and QT intervals become shorter, and the size of the infarct much smaller. Compared with NSAE, SSAE had more significant effects on the enzymatic activity of AST, LDH and the level of cTn I in serum. The H&E staining showed that both SSAE and NSAE could reduce the degree of heart damage.
Conclusions
The present investigation results demonstrated that sweating increased the content of tanshinone components in S. miltiorrhiza alcoholic extracts, and SSAE had a better protective effect on MIRI.
Graphical Abstract
Graphical Abstract</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1093/jpp/rgac012</identifier><language>eng</language><publisher>UK: Oxford University Press</publisher><ispartof>Journal of pharmacy and pharmacology, 2022-09, Vol.74 (9), p.1230-1240</ispartof><rights>The Author(s) 2022. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c297t-9aa649e083f89e703eb505b5c8e89920d3d8033c83ff7853750eb7a6c418da7f3</citedby><cites>FETCH-LOGICAL-c297t-9aa649e083f89e703eb505b5c8e89920d3d8033c83ff7853750eb7a6c418da7f3</cites><orcidid>0000-0002-7787-2638 ; 0000-0002-9570-0207 ; 0000-0003-3197-0635</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Shan, Xiaoxiao</creatorcontrib><creatorcontrib>Xiao, Yaoyao</creatorcontrib><creatorcontrib>Hong, Bangzhen</creatorcontrib><creatorcontrib>Li, Ling</creatorcontrib><creatorcontrib>Chen, Yueting</creatorcontrib><creatorcontrib>Wang, Guokai</creatorcontrib><creatorcontrib>Yu, Nianjun</creatorcontrib><creatorcontrib>Peng, Daiyin</creatorcontrib><creatorcontrib>Zhang, Caiyun</creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Chen, Weidong</creatorcontrib><title>Phytochemical profile and protective effects on myocardial ischaemia-reperfusion injury of sweated and non-sweated Salvia miltiorrhiza. Bge alcoholic extracts</title><title>Journal of pharmacy and pharmacology</title><description>Abstract
Objectives
This study aims to compare the fingerprint and the content of the three components of sweated and non-sweated Salvia miltiorrhiza alcoholic extracts (SSAE and NSAE). It also aims to investigate the difference in protective effects of SSAE and NSAE on myocardial ischaemia-reperfusion injury (MIRI).
Methods
The fingerprints of SSAE and NSAE were established by HPLC with a UV detector to identify the common peaks and detect the content of the three major components (cryptotanshinone, tanshinone I and tanshinone IIA). The protective effects of SSAE and NSAE were compared with MIRI rat model after orally administered SSAE and NSAE (2 g/kg of raw drug) for 7 days. The ST segment, PR and QT interval changes and the infarct size were assessed in the rat hearts. Moreover, the activity of aspartate transaminase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and the level of cardiac troponin I (cTn I) in serum as well as the cardiac H&E staining were evaluated.
Key findings
The results showed that the fingerprints of SSAE and NSAE were similar, and cluster analysis showed that the sweating methods had effects on the alcoholic extracts. The content determination showed that sweating could increase the total content of cryptotanshinone, tanshinone I and tanshinone IIA of S. miltiorrhiza. The results of electrocardiograms (ECG) showed that SSAE could make the ST segment drop more obviously, PR and QT intervals become shorter, and the size of the infarct much smaller. Compared with NSAE, SSAE had more significant effects on the enzymatic activity of AST, LDH and the level of cTn I in serum. The H&E staining showed that both SSAE and NSAE could reduce the degree of heart damage.
Conclusions
The present investigation results demonstrated that sweating increased the content of tanshinone components in S. miltiorrhiza alcoholic extracts, and SSAE had a better protective effect on MIRI.
Graphical Abstract
Graphical Abstract</description><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kc9O4zAQxi0EEuXPiRfwCSGtUhy7iZ0jixZYCWmRgHM0dcbElRMHO-lueRieFXcLV04zo_nN92n0EXKWs3nOKnG5GobL8AKa5XyPzDhb8EzmhdonM8Y4z0QhxSE5inHFGJNlWc7I-0O7Gb1usbMaHB2CN9Yhhb7Z9iPq0a6RojGpi9T3tNt4DaGxCbZRt5AOIQs4YDBTtAmw_WoKG-oNjX8RRmz-i_W-z77mR3BrC7SzbrQ-hNa-wZz-fEmuTvvWO6sp_hsDJMcTcmDARTz9rMfk-ebX0_Vddv_n9vf11X2meSXHrAIoFxUyJYyqUDKBy4IVy0IrVFXFWSMaxYTQaW-kKoQsGC4llHqRqwakEcfkYqebnn6dMI51l75D56BHP8Wal6oqJS8kT-iPHaqDjzGgqYdgOwibOmf1NoU6pVB_ppDo8x3tp-Fb8AMyxI1q</recordid><startdate>20220901</startdate><enddate>20220901</enddate><creator>Shan, Xiaoxiao</creator><creator>Xiao, Yaoyao</creator><creator>Hong, Bangzhen</creator><creator>Li, Ling</creator><creator>Chen, Yueting</creator><creator>Wang, Guokai</creator><creator>Yu, Nianjun</creator><creator>Peng, Daiyin</creator><creator>Zhang, Caiyun</creator><creator>Wang, Lei</creator><creator>Chen, Weidong</creator><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7787-2638</orcidid><orcidid>https://orcid.org/0000-0002-9570-0207</orcidid><orcidid>https://orcid.org/0000-0003-3197-0635</orcidid></search><sort><creationdate>20220901</creationdate><title>Phytochemical profile and protective effects on myocardial ischaemia-reperfusion injury of sweated and non-sweated Salvia miltiorrhiza. Bge alcoholic extracts</title><author>Shan, Xiaoxiao ; Xiao, Yaoyao ; Hong, Bangzhen ; Li, Ling ; Chen, Yueting ; Wang, Guokai ; Yu, Nianjun ; Peng, Daiyin ; Zhang, Caiyun ; Wang, Lei ; Chen, Weidong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c297t-9aa649e083f89e703eb505b5c8e89920d3d8033c83ff7853750eb7a6c418da7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shan, Xiaoxiao</creatorcontrib><creatorcontrib>Xiao, Yaoyao</creatorcontrib><creatorcontrib>Hong, Bangzhen</creatorcontrib><creatorcontrib>Li, Ling</creatorcontrib><creatorcontrib>Chen, Yueting</creatorcontrib><creatorcontrib>Wang, Guokai</creatorcontrib><creatorcontrib>Yu, Nianjun</creatorcontrib><creatorcontrib>Peng, Daiyin</creatorcontrib><creatorcontrib>Zhang, Caiyun</creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Chen, Weidong</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shan, Xiaoxiao</au><au>Xiao, Yaoyao</au><au>Hong, Bangzhen</au><au>Li, Ling</au><au>Chen, Yueting</au><au>Wang, Guokai</au><au>Yu, Nianjun</au><au>Peng, Daiyin</au><au>Zhang, Caiyun</au><au>Wang, Lei</au><au>Chen, Weidong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytochemical profile and protective effects on myocardial ischaemia-reperfusion injury of sweated and non-sweated Salvia miltiorrhiza. Bge alcoholic extracts</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><date>2022-09-01</date><risdate>2022</risdate><volume>74</volume><issue>9</issue><spage>1230</spage><epage>1240</epage><pages>1230-1240</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><abstract>Abstract
Objectives
This study aims to compare the fingerprint and the content of the three components of sweated and non-sweated Salvia miltiorrhiza alcoholic extracts (SSAE and NSAE). It also aims to investigate the difference in protective effects of SSAE and NSAE on myocardial ischaemia-reperfusion injury (MIRI).
Methods
The fingerprints of SSAE and NSAE were established by HPLC with a UV detector to identify the common peaks and detect the content of the three major components (cryptotanshinone, tanshinone I and tanshinone IIA). The protective effects of SSAE and NSAE were compared with MIRI rat model after orally administered SSAE and NSAE (2 g/kg of raw drug) for 7 days. The ST segment, PR and QT interval changes and the infarct size were assessed in the rat hearts. Moreover, the activity of aspartate transaminase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and the level of cardiac troponin I (cTn I) in serum as well as the cardiac H&E staining were evaluated.
Key findings
The results showed that the fingerprints of SSAE and NSAE were similar, and cluster analysis showed that the sweating methods had effects on the alcoholic extracts. The content determination showed that sweating could increase the total content of cryptotanshinone, tanshinone I and tanshinone IIA of S. miltiorrhiza. The results of electrocardiograms (ECG) showed that SSAE could make the ST segment drop more obviously, PR and QT intervals become shorter, and the size of the infarct much smaller. Compared with NSAE, SSAE had more significant effects on the enzymatic activity of AST, LDH and the level of cTn I in serum. The H&E staining showed that both SSAE and NSAE could reduce the degree of heart damage.
Conclusions
The present investigation results demonstrated that sweating increased the content of tanshinone components in S. miltiorrhiza alcoholic extracts, and SSAE had a better protective effect on MIRI.
Graphical Abstract
Graphical Abstract</abstract><cop>UK</cop><pub>Oxford University Press</pub><doi>10.1093/jpp/rgac012</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-7787-2638</orcidid><orcidid>https://orcid.org/0000-0002-9570-0207</orcidid><orcidid>https://orcid.org/0000-0003-3197-0635</orcidid></addata></record> |
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| title | Phytochemical profile and protective effects on myocardial ischaemia-reperfusion injury of sweated and non-sweated Salvia miltiorrhiza. Bge alcoholic extracts |
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