High-Level De Novo Biosynthesis of 2′-Fucosyllactose by Metabolically Engineered Escherichia coli

2′-Fucosyllactose (2′-FL) is the most abundant oligosaccharide in human milk. In this study, a highly efficient biosynthetic route for 2′-FL production was designed via the de novo pathway of GDP-l-fucose using engineered Escherichia coli BL21­(DE3). Specifically, plasmid-based strains with previous...

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Veröffentlicht in:Journal of agricultural and food chemistry 2022-07, Vol.70 (29), p.9017-9025
Hauptverfasser: Liu, Yuanlin, Zhu, Yingying, Wan, Li, Chen, Roulin, Zhang, Wenli, Mu, Wanmeng
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Sprache:eng
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