Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes

The crystal structures of cattleyene synthase (apo‐CyS), and CyS complexed with geranylgeranyl pyrophosphate (GGPP) were solved. The CySC59A variant exhibited an increased production of cattleyene and other diterpenes with diverse skeletons. Its structure showed a widened active site cavity explaini...

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Veröffentlicht in:	Angewandte Chemie International Edition 2022-09, Vol.61 (36), p.e202209785-n/a
Hauptverfasser:	Xing, Baiying, Xu, Houchao, Li, Annan, Lou, Tingting, Xu, Meng, Wang, Kaibiao, Xu, Zhengren, Dickschat, Jeroen S., Yang, Donghui, Ma, Ming
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosynthesis Catalytic Domain Crystal structure Cyclization Diterpenes Diterpenes - chemistry Enzymes Isotopic labeling Mutagenesis Natural Products Radioactive labeling Reaction Mechanisms Selectivity
Online-Zugang:	Volltext
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