A study of risk factors associated with Newcastle disease and molecular characterization of genotype XIII Newcastle disease virus in backyard and commercial poultry in Assam, India
The continuous emergence of Newcastle disease virus (NDV) poses a persistent threat to the poultry industry. Recent increasing outbreaks of NDV in the North East region of India have highlighted the need to closely monitor and analyze the potential risk factors for Newcastle disease (ND) outbreaks....
Gespeichert in:
| Veröffentlicht in: | Research in veterinary science 2022-12, Vol.150, p.122-130 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 130 |
|---|---|
| container_issue | |
| container_start_page | 122 |
| container_title | Research in veterinary science |
| container_volume | 150 |
| creator | Deka, Pubaleem Nath, Mrinal Kumar Das, Sangeeta Das, Bipin Chandra Phukan, Arabinda Lahkar, Deepa Bora, Birina Shokeen, Kamal Kumar, Aman Deka, Pankaj |
| description | The continuous emergence of Newcastle disease virus (NDV) poses a persistent threat to the poultry industry. Recent increasing outbreaks of NDV in the North East region of India have highlighted the need to closely monitor and analyze the potential risk factors for Newcastle disease (ND) outbreaks. In the present study, an attempt was made to genotype the circulating Newcastle disease virus (NDV) in the backyard and commercial poultry flocks in Assam, India. Sera samples from unvaccinated backyard poultry flocks and tissue samples of ND suspected cases were collected and tested for the presence of NDV antibodies using the Haemagglutination inhibition (HI) test. A total of seven NDV isolates were analyzed from different districts of Assam, India, both genotypically and pathotypically. All isolates were characterized as virulent, carrying 112RRKQRF117 amino acid residues at the cleavage site. As determined by phylogenetic analysis, the isolates clustered with members of genotype XIII of class II NDV. Further analysis of risk factors of ND occurrence was conducted through a questionnaire survey. All the results indicated an occurrence of genotype XIII of NDV in the farms with inadequate biosecurity and farming practices.
•Seroprevalence of ND in Assam, India is 23.89%.•The MDT and ICPI of circulating NDV strains were found to be 76.0 ± 2.69 to 87.6 ± 1.00 h and 1.05 ± 0.02 to 1.37 ± 0.02 respectively.•A phylogenetic analysis based on partial F gene sequences showed that the NDV isolates clustered with members of genotype XIII of class II NDV.•Studies revealed that the risk factors related to biosecurity and farm practices appear to have a significant role in the severity of Newcastle disease in poultry. |
| doi_str_mv | 10.1016/j.rvsc.2022.04.018 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2688522734</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0034528822001795</els_id><sourcerecordid>2702998153</sourcerecordid><originalsourceid>FETCH-LOGICAL-c361t-bf6c9c3fa084d3e77b847bfcc7956abbf290a426e49cf9085bf0f6f53c840fe83</originalsourceid><addsrcrecordid>eNp9kT2P1DAQhiMEEsvBH6CyRENBgu04iSPRrE58rHSCBiQ6y5mMOe8l8eJx9hR-Fz-QhKVCiGqa531nRk-WPRe8EFzUr49FPBMUkktZcFVwoR9kO1GVMpd1LR5mO85LlVdS68fZE6Ij51wJ0eyyn3tGae4XFhyLnu6Ys5BCJGaJAnibsGf3Pt2yj3gPltKArPeElpDZqWdjGBDmwUYGtzauUYz-h00-TFvhN5xCWk7Ivh4Oh380nH2cifmJdRbuFhv7350QxhHjuntgpzAPKS4bsiey4yt2mHpvn2aPnB0In_2ZV9mXd28_X3_Ibz69P1zvb3Ioa5HyztXQQuks16ovsWk6rZrOATRtVduuc7LlVskaVQuu5brqHHe1q0rQijvU5VX28tJ7iuH7jJTM6AlwGOyEYSYja60rKZtSreiLv9BjmOO0Xmdkw2Xb6tXGSskLBTEQRXTmFP1o42IEN5tIczSbSLOJNFyZVeQaenMJ4frq2WM0BB4nwN5HhGT64P8X_wX466sF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2702998153</pqid></control><display><type>article</type><title>A study of risk factors associated with Newcastle disease and molecular characterization of genotype XIII Newcastle disease virus in backyard and commercial poultry in Assam, India</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Deka, Pubaleem ; Nath, Mrinal Kumar ; Das, Sangeeta ; Das, Bipin Chandra ; Phukan, Arabinda ; Lahkar, Deepa ; Bora, Birina ; Shokeen, Kamal ; Kumar, Aman ; Deka, Pankaj</creator><creatorcontrib>Deka, Pubaleem ; Nath, Mrinal Kumar ; Das, Sangeeta ; Das, Bipin Chandra ; Phukan, Arabinda ; Lahkar, Deepa ; Bora, Birina ; Shokeen, Kamal ; Kumar, Aman ; Deka, Pankaj</creatorcontrib><description>The continuous emergence of Newcastle disease virus (NDV) poses a persistent threat to the poultry industry. Recent increasing outbreaks of NDV in the North East region of India have highlighted the need to closely monitor and analyze the potential risk factors for Newcastle disease (ND) outbreaks. In the present study, an attempt was made to genotype the circulating Newcastle disease virus (NDV) in the backyard and commercial poultry flocks in Assam, India. Sera samples from unvaccinated backyard poultry flocks and tissue samples of ND suspected cases were collected and tested for the presence of NDV antibodies using the Haemagglutination inhibition (HI) test. A total of seven NDV isolates were analyzed from different districts of Assam, India, both genotypically and pathotypically. All isolates were characterized as virulent, carrying 112RRKQRF117 amino acid residues at the cleavage site. As determined by phylogenetic analysis, the isolates clustered with members of genotype XIII of class II NDV. Further analysis of risk factors of ND occurrence was conducted through a questionnaire survey. All the results indicated an occurrence of genotype XIII of NDV in the farms with inadequate biosecurity and farming practices.
•Seroprevalence of ND in Assam, India is 23.89%.•The MDT and ICPI of circulating NDV strains were found to be 76.0 ± 2.69 to 87.6 ± 1.00 h and 1.05 ± 0.02 to 1.37 ± 0.02 respectively.•A phylogenetic analysis based on partial F gene sequences showed that the NDV isolates clustered with members of genotype XIII of class II NDV.•Studies revealed that the risk factors related to biosecurity and farm practices appear to have a significant role in the severity of Newcastle disease in poultry.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2022.04.018</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Agricultural practices ; Amino acids ; Antibodies ; Biosecurity ; Birds ; Epidemics ; Farms ; Fusion protein ; Genomes ; Genotype & phenotype ; Genotype XIII ; Genotypes ; Health risks ; Hemagglutination inhibition ; Major risk factors ; Newcastle disease ; Outbreaks ; Phylogenetic analysis ; Phylogenetics ; Phylogeny ; Poultry ; Proteins ; Risk analysis ; Risk factors ; Serology ; Seroprevalence ; Vaccines ; Veterinary medicine ; Virulence ; Viruses</subject><ispartof>Research in veterinary science, 2022-12, Vol.150, p.122-130</ispartof><rights>2022</rights><rights>Copyright Elsevier Limited Dec 5, 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-bf6c9c3fa084d3e77b847bfcc7956abbf290a426e49cf9085bf0f6f53c840fe83</citedby><cites>FETCH-LOGICAL-c361t-bf6c9c3fa084d3e77b847bfcc7956abbf290a426e49cf9085bf0f6f53c840fe83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2022.04.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Deka, Pubaleem</creatorcontrib><creatorcontrib>Nath, Mrinal Kumar</creatorcontrib><creatorcontrib>Das, Sangeeta</creatorcontrib><creatorcontrib>Das, Bipin Chandra</creatorcontrib><creatorcontrib>Phukan, Arabinda</creatorcontrib><creatorcontrib>Lahkar, Deepa</creatorcontrib><creatorcontrib>Bora, Birina</creatorcontrib><creatorcontrib>Shokeen, Kamal</creatorcontrib><creatorcontrib>Kumar, Aman</creatorcontrib><creatorcontrib>Deka, Pankaj</creatorcontrib><title>A study of risk factors associated with Newcastle disease and molecular characterization of genotype XIII Newcastle disease virus in backyard and commercial poultry in Assam, India</title><title>Research in veterinary science</title><description>The continuous emergence of Newcastle disease virus (NDV) poses a persistent threat to the poultry industry. Recent increasing outbreaks of NDV in the North East region of India have highlighted the need to closely monitor and analyze the potential risk factors for Newcastle disease (ND) outbreaks. In the present study, an attempt was made to genotype the circulating Newcastle disease virus (NDV) in the backyard and commercial poultry flocks in Assam, India. Sera samples from unvaccinated backyard poultry flocks and tissue samples of ND suspected cases were collected and tested for the presence of NDV antibodies using the Haemagglutination inhibition (HI) test. A total of seven NDV isolates were analyzed from different districts of Assam, India, both genotypically and pathotypically. All isolates were characterized as virulent, carrying 112RRKQRF117 amino acid residues at the cleavage site. As determined by phylogenetic analysis, the isolates clustered with members of genotype XIII of class II NDV. Further analysis of risk factors of ND occurrence was conducted through a questionnaire survey. All the results indicated an occurrence of genotype XIII of NDV in the farms with inadequate biosecurity and farming practices.
•Seroprevalence of ND in Assam, India is 23.89%.•The MDT and ICPI of circulating NDV strains were found to be 76.0 ± 2.69 to 87.6 ± 1.00 h and 1.05 ± 0.02 to 1.37 ± 0.02 respectively.•A phylogenetic analysis based on partial F gene sequences showed that the NDV isolates clustered with members of genotype XIII of class II NDV.•Studies revealed that the risk factors related to biosecurity and farm practices appear to have a significant role in the severity of Newcastle disease in poultry.</description><subject>Agricultural practices</subject><subject>Amino acids</subject><subject>Antibodies</subject><subject>Biosecurity</subject><subject>Birds</subject><subject>Epidemics</subject><subject>Farms</subject><subject>Fusion protein</subject><subject>Genomes</subject><subject>Genotype & phenotype</subject><subject>Genotype XIII</subject><subject>Genotypes</subject><subject>Health risks</subject><subject>Hemagglutination inhibition</subject><subject>Major risk factors</subject><subject>Newcastle disease</subject><subject>Outbreaks</subject><subject>Phylogenetic analysis</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Poultry</subject><subject>Proteins</subject><subject>Risk analysis</subject><subject>Risk factors</subject><subject>Serology</subject><subject>Seroprevalence</subject><subject>Vaccines</subject><subject>Veterinary medicine</subject><subject>Virulence</subject><subject>Viruses</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kT2P1DAQhiMEEsvBH6CyRENBgu04iSPRrE58rHSCBiQ6y5mMOe8l8eJx9hR-Fz-QhKVCiGqa531nRk-WPRe8EFzUr49FPBMUkktZcFVwoR9kO1GVMpd1LR5mO85LlVdS68fZE6Ij51wJ0eyyn3tGae4XFhyLnu6Ys5BCJGaJAnibsGf3Pt2yj3gPltKArPeElpDZqWdjGBDmwUYGtzauUYz-h00-TFvhN5xCWk7Ivh4Oh380nH2cifmJdRbuFhv7350QxhHjuntgpzAPKS4bsiey4yt2mHpvn2aPnB0In_2ZV9mXd28_X3_Ibz69P1zvb3Ioa5HyztXQQuks16ovsWk6rZrOATRtVduuc7LlVskaVQuu5brqHHe1q0rQijvU5VX28tJ7iuH7jJTM6AlwGOyEYSYja60rKZtSreiLv9BjmOO0Xmdkw2Xb6tXGSskLBTEQRXTmFP1o42IEN5tIczSbSLOJNFyZVeQaenMJ4frq2WM0BB4nwN5HhGT64P8X_wX466sF</recordid><startdate>20221205</startdate><enddate>20221205</enddate><creator>Deka, Pubaleem</creator><creator>Nath, Mrinal Kumar</creator><creator>Das, Sangeeta</creator><creator>Das, Bipin Chandra</creator><creator>Phukan, Arabinda</creator><creator>Lahkar, Deepa</creator><creator>Bora, Birina</creator><creator>Shokeen, Kamal</creator><creator>Kumar, Aman</creator><creator>Deka, Pankaj</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20221205</creationdate><title>A study of risk factors associated with Newcastle disease and molecular characterization of genotype XIII Newcastle disease virus in backyard and commercial poultry in Assam, India</title><author>Deka, Pubaleem ; Nath, Mrinal Kumar ; Das, Sangeeta ; Das, Bipin Chandra ; Phukan, Arabinda ; Lahkar, Deepa ; Bora, Birina ; Shokeen, Kamal ; Kumar, Aman ; Deka, Pankaj</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-bf6c9c3fa084d3e77b847bfcc7956abbf290a426e49cf9085bf0f6f53c840fe83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Agricultural practices</topic><topic>Amino acids</topic><topic>Antibodies</topic><topic>Biosecurity</topic><topic>Birds</topic><topic>Epidemics</topic><topic>Farms</topic><topic>Fusion protein</topic><topic>Genomes</topic><topic>Genotype & phenotype</topic><topic>Genotype XIII</topic><topic>Genotypes</topic><topic>Health risks</topic><topic>Hemagglutination inhibition</topic><topic>Major risk factors</topic><topic>Newcastle disease</topic><topic>Outbreaks</topic><topic>Phylogenetic analysis</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Poultry</topic><topic>Proteins</topic><topic>Risk analysis</topic><topic>Risk factors</topic><topic>Serology</topic><topic>Seroprevalence</topic><topic>Vaccines</topic><topic>Veterinary medicine</topic><topic>Virulence</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deka, Pubaleem</creatorcontrib><creatorcontrib>Nath, Mrinal Kumar</creatorcontrib><creatorcontrib>Das, Sangeeta</creatorcontrib><creatorcontrib>Das, Bipin Chandra</creatorcontrib><creatorcontrib>Phukan, Arabinda</creatorcontrib><creatorcontrib>Lahkar, Deepa</creatorcontrib><creatorcontrib>Bora, Birina</creatorcontrib><creatorcontrib>Shokeen, Kamal</creatorcontrib><creatorcontrib>Kumar, Aman</creatorcontrib><creatorcontrib>Deka, Pankaj</creatorcontrib><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deka, Pubaleem</au><au>Nath, Mrinal Kumar</au><au>Das, Sangeeta</au><au>Das, Bipin Chandra</au><au>Phukan, Arabinda</au><au>Lahkar, Deepa</au><au>Bora, Birina</au><au>Shokeen, Kamal</au><au>Kumar, Aman</au><au>Deka, Pankaj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A study of risk factors associated with Newcastle disease and molecular characterization of genotype XIII Newcastle disease virus in backyard and commercial poultry in Assam, India</atitle><jtitle>Research in veterinary science</jtitle><date>2022-12-05</date><risdate>2022</risdate><volume>150</volume><spage>122</spage><epage>130</epage><pages>122-130</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>The continuous emergence of Newcastle disease virus (NDV) poses a persistent threat to the poultry industry. Recent increasing outbreaks of NDV in the North East region of India have highlighted the need to closely monitor and analyze the potential risk factors for Newcastle disease (ND) outbreaks. In the present study, an attempt was made to genotype the circulating Newcastle disease virus (NDV) in the backyard and commercial poultry flocks in Assam, India. Sera samples from unvaccinated backyard poultry flocks and tissue samples of ND suspected cases were collected and tested for the presence of NDV antibodies using the Haemagglutination inhibition (HI) test. A total of seven NDV isolates were analyzed from different districts of Assam, India, both genotypically and pathotypically. All isolates were characterized as virulent, carrying 112RRKQRF117 amino acid residues at the cleavage site. As determined by phylogenetic analysis, the isolates clustered with members of genotype XIII of class II NDV. Further analysis of risk factors of ND occurrence was conducted through a questionnaire survey. All the results indicated an occurrence of genotype XIII of NDV in the farms with inadequate biosecurity and farming practices.
•Seroprevalence of ND in Assam, India is 23.89%.•The MDT and ICPI of circulating NDV strains were found to be 76.0 ± 2.69 to 87.6 ± 1.00 h and 1.05 ± 0.02 to 1.37 ± 0.02 respectively.•A phylogenetic analysis based on partial F gene sequences showed that the NDV isolates clustered with members of genotype XIII of class II NDV.•Studies revealed that the risk factors related to biosecurity and farm practices appear to have a significant role in the severity of Newcastle disease in poultry.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.rvsc.2022.04.018</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0034-5288 |
| ispartof | Research in veterinary science, 2022-12, Vol.150, p.122-130 |
| issn | 0034-5288 1532-2661 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2688522734 |
| source | Elsevier ScienceDirect Journals Complete |
| subjects | Agricultural practices Amino acids Antibodies Biosecurity Birds Epidemics Farms Fusion protein Genomes Genotype & phenotype Genotype XIII Genotypes Health risks Hemagglutination inhibition Major risk factors Newcastle disease Outbreaks Phylogenetic analysis Phylogenetics Phylogeny Poultry Proteins Risk analysis Risk factors Serology Seroprevalence Vaccines Veterinary medicine Virulence Viruses |
| title | A study of risk factors associated with Newcastle disease and molecular characterization of genotype XIII Newcastle disease virus in backyard and commercial poultry in Assam, India |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T12%3A41%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20study%20of%20risk%20factors%20associated%20with%20Newcastle%20disease%20and%20molecular%20characterization%20of%20genotype%20XIII%20Newcastle%20disease%20virus%20in%20backyard%20and%20commercial%20poultry%20in%20Assam,%20India&rft.jtitle=Research%20in%20veterinary%20science&rft.au=Deka,%20Pubaleem&rft.date=2022-12-05&rft.volume=150&rft.spage=122&rft.epage=130&rft.pages=122-130&rft.issn=0034-5288&rft.eissn=1532-2661&rft_id=info:doi/10.1016/j.rvsc.2022.04.018&rft_dat=%3Cproquest_cross%3E2702998153%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2702998153&rft_id=info:pmid/&rft_els_id=S0034528822001795&rfr_iscdi=true |