Reassessing acetyl‐CoA supply and NADPH availability for mevalonate biosynthesis from glycerol in Escherichia coli
Mevalonate is an important platform compound for the biosynthesis of isoprenoids. It can be synthesized from acetyl‐CoA in the presence of nicotinamide adenine dinucleotide phosphate (NADPH) by the introduced mvaES operon in Escherichia coli. The influences of E. coli hosts, acetyl‐CoA supply, and N...
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| Veröffentlicht in: | Biotechnology and bioengineering 2022-10, Vol.119 (10), p.2868-2877 |
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| container_title | Biotechnology and bioengineering |
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| creator | Wang, Yan Zhou, Shenting Li, Runyi Liu, Qian Shao, Xixi Zhu, Liyan Kang, Min‐Kyoung Wei, Gongyuan Kim, Seon‐Won Wang, Chonglong |
| description | Mevalonate is an important platform compound for the biosynthesis of isoprenoids. It can be synthesized from acetyl‐CoA in the presence of nicotinamide adenine dinucleotide phosphate (NADPH) by the introduced mvaES operon in Escherichia coli. The influences of E. coli hosts, acetyl‐CoA supply, and NADPH availability were assessed and engineered to improve the production titer and yield of mevalonate from glycerol. As a result, E. coli DH5α was found to be the best host with high specific capability and titer of mevalonate from glycerol. Through the engineering of phosphoketolase‐phosphotransacetylase (xPK‐PTA) bypass and NADPH availability, a final titer of 7.21 g/L with a specific capability of 1.36 g/g dry cell weight was gained in flask culture. Our work could offer new information to metabolically engineer the mevalonate pathway for the efficient production of isoprenoids.
The effective acetyl‐CoA supply and nicotinamide adenine dinucleotide phosphate availability boost mevalonate biosynthesis from glycerol in Escherichia coli. |
| doi_str_mv | 10.1002/bit.28167 |
| format | Article |
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The effective acetyl‐CoA supply and nicotinamide adenine dinucleotide phosphate availability boost mevalonate biosynthesis from glycerol in Escherichia coli.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.28167</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc</publisher><subject>acetyl‐CoA ; Adenine ; Availability ; Biosynthesis ; Cell culture ; Dry cells ; E coli ; Escherichia coli ; Glycerol ; mevalonate ; Mevalonate pathway ; Mevalonic acid ; NADPH ; NADPH-diaphorase ; Nicotinamide ; Nicotinamide adenine dinucleotide ; Phosphoketolase ; Terpenes</subject><ispartof>Biotechnology and bioengineering, 2022-10, Vol.119 (10), p.2868-2877</ispartof><rights>2022 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3307-18b39d3342579d0474c4efac36c933d8daf0d9abb09d7ce9f2c2683d2093d8ee3</citedby><cites>FETCH-LOGICAL-c3307-18b39d3342579d0474c4efac36c933d8daf0d9abb09d7ce9f2c2683d2093d8ee3</cites><orcidid>0000-0001-5420-0743</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.28167$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.28167$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhou, Shenting</creatorcontrib><creatorcontrib>Li, Runyi</creatorcontrib><creatorcontrib>Liu, Qian</creatorcontrib><creatorcontrib>Shao, Xixi</creatorcontrib><creatorcontrib>Zhu, Liyan</creatorcontrib><creatorcontrib>Kang, Min‐Kyoung</creatorcontrib><creatorcontrib>Wei, Gongyuan</creatorcontrib><creatorcontrib>Kim, Seon‐Won</creatorcontrib><creatorcontrib>Wang, Chonglong</creatorcontrib><title>Reassessing acetyl‐CoA supply and NADPH availability for mevalonate biosynthesis from glycerol in Escherichia coli</title><title>Biotechnology and bioengineering</title><description>Mevalonate is an important platform compound for the biosynthesis of isoprenoids. It can be synthesized from acetyl‐CoA in the presence of nicotinamide adenine dinucleotide phosphate (NADPH) by the introduced mvaES operon in Escherichia coli. The influences of E. coli hosts, acetyl‐CoA supply, and NADPH availability were assessed and engineered to improve the production titer and yield of mevalonate from glycerol. As a result, E. coli DH5α was found to be the best host with high specific capability and titer of mevalonate from glycerol. Through the engineering of phosphoketolase‐phosphotransacetylase (xPK‐PTA) bypass and NADPH availability, a final titer of 7.21 g/L with a specific capability of 1.36 g/g dry cell weight was gained in flask culture. Our work could offer new information to metabolically engineer the mevalonate pathway for the efficient production of isoprenoids.
The effective acetyl‐CoA supply and nicotinamide adenine dinucleotide phosphate availability boost mevalonate biosynthesis from glycerol in Escherichia coli.</description><subject>acetyl‐CoA</subject><subject>Adenine</subject><subject>Availability</subject><subject>Biosynthesis</subject><subject>Cell culture</subject><subject>Dry cells</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Glycerol</subject><subject>mevalonate</subject><subject>Mevalonate pathway</subject><subject>Mevalonic acid</subject><subject>NADPH</subject><subject>NADPH-diaphorase</subject><subject>Nicotinamide</subject><subject>Nicotinamide adenine dinucleotide</subject><subject>Phosphoketolase</subject><subject>Terpenes</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kE1u2zAQRomgBeK6XeQGBLppF7IpUhbFpev8OECQBEWyJihqFDOgRZcjOdAuR8gZc5KwdVYFshoM5n0fBo-Qk5zNcsb4vHb9jFd5KY_IJGdKZowr9olMGGNlJhaKH5MviI9plVVZTkj_GwwiILrugRoL_ehfn19WYUlx2O38SE3X0Ovl6e2amr1x3tTOu36kbYh0C3vjQ2d6oLULOHb9BtAhbWPY0gc_WojBU9fRM7QbiM5unKE2ePeVfG6NR_j2Pqfk_vzsbrXOrm4uLlfLq8wKwWSWV7VQjRAFX0jVsEIWtoDWWFFaJURTNaZljTJ1zVQjLaiWW15WouFMpSuAmJIfh95dDH8GwF5vHVrw3nQQBtSJXjAhFoVM6Pf_0McwxC59p7lMIvMiT9VT8vNA2RgQI7R6F93WxFHnTP_1r5N__c9_YucH9sl5GD8G9a_Lu0PiDSoYiQM</recordid><startdate>202210</startdate><enddate>202210</enddate><creator>Wang, Yan</creator><creator>Zhou, Shenting</creator><creator>Li, Runyi</creator><creator>Liu, Qian</creator><creator>Shao, Xixi</creator><creator>Zhu, Liyan</creator><creator>Kang, Min‐Kyoung</creator><creator>Wei, Gongyuan</creator><creator>Kim, Seon‐Won</creator><creator>Wang, Chonglong</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5420-0743</orcidid></search><sort><creationdate>202210</creationdate><title>Reassessing acetyl‐CoA supply and NADPH availability for mevalonate biosynthesis from glycerol in Escherichia coli</title><author>Wang, Yan ; Zhou, Shenting ; Li, Runyi ; Liu, Qian ; Shao, Xixi ; Zhu, Liyan ; Kang, Min‐Kyoung ; Wei, Gongyuan ; Kim, Seon‐Won ; Wang, Chonglong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3307-18b39d3342579d0474c4efac36c933d8daf0d9abb09d7ce9f2c2683d2093d8ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>acetyl‐CoA</topic><topic>Adenine</topic><topic>Availability</topic><topic>Biosynthesis</topic><topic>Cell culture</topic><topic>Dry cells</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Glycerol</topic><topic>mevalonate</topic><topic>Mevalonate pathway</topic><topic>Mevalonic acid</topic><topic>NADPH</topic><topic>NADPH-diaphorase</topic><topic>Nicotinamide</topic><topic>Nicotinamide adenine dinucleotide</topic><topic>Phosphoketolase</topic><topic>Terpenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhou, Shenting</creatorcontrib><creatorcontrib>Li, Runyi</creatorcontrib><creatorcontrib>Liu, Qian</creatorcontrib><creatorcontrib>Shao, Xixi</creatorcontrib><creatorcontrib>Zhu, Liyan</creatorcontrib><creatorcontrib>Kang, Min‐Kyoung</creatorcontrib><creatorcontrib>Wei, Gongyuan</creatorcontrib><creatorcontrib>Kim, Seon‐Won</creatorcontrib><creatorcontrib>Wang, Chonglong</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yan</au><au>Zhou, Shenting</au><au>Li, Runyi</au><au>Liu, Qian</au><au>Shao, Xixi</au><au>Zhu, Liyan</au><au>Kang, Min‐Kyoung</au><au>Wei, Gongyuan</au><au>Kim, Seon‐Won</au><au>Wang, Chonglong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reassessing acetyl‐CoA supply and NADPH availability for mevalonate biosynthesis from glycerol in Escherichia coli</atitle><jtitle>Biotechnology and bioengineering</jtitle><date>2022-10</date><risdate>2022</risdate><volume>119</volume><issue>10</issue><spage>2868</spage><epage>2877</epage><pages>2868-2877</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><abstract>Mevalonate is an important platform compound for the biosynthesis of isoprenoids. It can be synthesized from acetyl‐CoA in the presence of nicotinamide adenine dinucleotide phosphate (NADPH) by the introduced mvaES operon in Escherichia coli. The influences of E. coli hosts, acetyl‐CoA supply, and NADPH availability were assessed and engineered to improve the production titer and yield of mevalonate from glycerol. As a result, E. coli DH5α was found to be the best host with high specific capability and titer of mevalonate from glycerol. Through the engineering of phosphoketolase‐phosphotransacetylase (xPK‐PTA) bypass and NADPH availability, a final titer of 7.21 g/L with a specific capability of 1.36 g/g dry cell weight was gained in flask culture. Our work could offer new information to metabolically engineer the mevalonate pathway for the efficient production of isoprenoids.
The effective acetyl‐CoA supply and nicotinamide adenine dinucleotide phosphate availability boost mevalonate biosynthesis from glycerol in Escherichia coli.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/bit.28167</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5420-0743</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | acetyl‐CoA Adenine Availability Biosynthesis Cell culture Dry cells E coli Escherichia coli Glycerol mevalonate Mevalonate pathway Mevalonic acid NADPH NADPH-diaphorase Nicotinamide Nicotinamide adenine dinucleotide Phosphoketolase Terpenes |
| title | Reassessing acetyl‐CoA supply and NADPH availability for mevalonate biosynthesis from glycerol in Escherichia coli |
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