Occurrence of blaNDM-7 and association with blaKPC-2, blaCTX-M15, aac, aph, mph(A), catB3 and virulence genes in a clinical isolate of Klebsiella pneumoniae with different plasmids in Brazil
To characterize phenotypically and genotypically an isolate of multidrug-resistant (MDR) K. pneumoniae from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated using PCR and sequencing the amplicons, and the plasmid DNA was also sequenced. T...
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| Veröffentlicht in: | Archives of microbiology 2022-08, Vol.204 (8), p.459-459, Article 459 |
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| creator | de Oliveira Alves, Weverton Scavuzzi, Alexsandra Maria Lima Beltrão, Elizabeth Maria Bispo de Oliveira, Érica Maria dos Santos Vasconcelos, Crhisllane Rafaele Rezende, Antônio Mauro de Souza Lopes, Ana Catarina |
| description | To characterize phenotypically and genotypically an isolate of multidrug-resistant (MDR)
K. pneumoniae
from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated using PCR and sequencing the amplicons, and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all
β
-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variants
bla
NDM
-7
associated with
bla
KPC-2
and the
cps
,
wabG
,
fim-H
,
mrkD
and
entB
virulence genes were identified. The analysis of plasmid revealed the presence of
bla
CTX-M15
,
aac(3)-IVa
,
aph(3′)-Ia
,
aph(4)-Ia
,
aac(6′)ib-cr
,
mph(A)
and
catB3
, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the
bla
NDM
-7
gene in Recife-PE and we suggest that this variant is located in IncX3
.
These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes. |
| doi_str_mv | 10.1007/s00203-022-03051-0 |
| format | Article |
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K. pneumoniae
from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated using PCR and sequencing the amplicons, and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all
β
-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variants
bla
NDM
-7
associated with
bla
KPC-2
and the
cps
,
wabG
,
fim-H
,
mrkD
and
entB
virulence genes were identified. The analysis of plasmid revealed the presence of
bla
CTX-M15
,
aac(3)-IVa
,
aph(3′)-Ia
,
aph(4)-Ia
,
aac(6′)ib-cr
,
mph(A)
and
catB3
, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the
bla
NDM
-7
gene in Recife-PE and we suggest that this variant is located in IncX3
.
These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-022-03051-0</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aminoglycosides ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Carbapenems ; Cell Biology ; Ecology ; Gene sequencing ; Genes ; Horizontal transfer ; Klebsiella ; Life Sciences ; Microbial Ecology ; Microbiology ; Multidrug resistance ; Nucleotide sequence ; Plasmids ; Polymerase chain reaction ; Quinolones ; Septicemia ; Short Communication ; Virulence ; β-Lactam antibiotics</subject><ispartof>Archives of microbiology, 2022-08, Vol.204 (8), p.459-459, Article 459</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1970-ce1de4c440310917ee06dbe277670050444df9944f62b0af734b870e103bc2203</citedby><cites>FETCH-LOGICAL-c1970-ce1de4c440310917ee06dbe277670050444df9944f62b0af734b870e103bc2203</cites><orcidid>0000-0001-7821-1068 ; 0000-0003-4195-4380 ; 0000-0002-9650-7900 ; 0000-0001-8290-5775 ; 0000-0003-4775-1779 ; 0000-0003-0277-108X ; 0000-0001-8780-3460</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-022-03051-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-022-03051-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>de Oliveira Alves, Weverton</creatorcontrib><creatorcontrib>Scavuzzi, Alexsandra Maria Lima</creatorcontrib><creatorcontrib>Beltrão, Elizabeth Maria Bispo</creatorcontrib><creatorcontrib>de Oliveira, Érica Maria</creatorcontrib><creatorcontrib>dos Santos Vasconcelos, Crhisllane Rafaele</creatorcontrib><creatorcontrib>Rezende, Antônio Mauro</creatorcontrib><creatorcontrib>de Souza Lopes, Ana Catarina</creatorcontrib><title>Occurrence of blaNDM-7 and association with blaKPC-2, blaCTX-M15, aac, aph, mph(A), catB3 and virulence genes in a clinical isolate of Klebsiella pneumoniae with different plasmids in Brazil</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><description>To characterize phenotypically and genotypically an isolate of multidrug-resistant (MDR)
K. pneumoniae
from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated using PCR and sequencing the amplicons, and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all
β
-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variants
bla
NDM
-7
associated with
bla
KPC-2
and the
cps
,
wabG
,
fim-H
,
mrkD
and
entB
virulence genes were identified. The analysis of plasmid revealed the presence of
bla
CTX-M15
,
aac(3)-IVa
,
aph(3′)-Ia
,
aph(4)-Ia
,
aac(6′)ib-cr
,
mph(A)
and
catB3
, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the
bla
NDM
-7
gene in Recife-PE and we suggest that this variant is located in IncX3
.
These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes.</description><subject>Aminoglycosides</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Carbapenems</subject><subject>Cell Biology</subject><subject>Ecology</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Horizontal transfer</subject><subject>Klebsiella</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Multidrug resistance</subject><subject>Nucleotide sequence</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Quinolones</subject><subject>Septicemia</subject><subject>Short Communication</subject><subject>Virulence</subject><subject>β-Lactam 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Maria</creator><creator>dos Santos Vasconcelos, Crhisllane Rafaele</creator><creator>Rezende, Antônio Mauro</creator><creator>de Souza Lopes, Ana Catarina</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature 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of blaNDM-7 and association with blaKPC-2, blaCTX-M15, aac, aph, mph(A), catB3 and virulence genes in a clinical isolate of Klebsiella pneumoniae with different plasmids in Brazil</title><author>de Oliveira Alves, Weverton ; Scavuzzi, Alexsandra Maria Lima ; Beltrão, Elizabeth Maria Bispo ; de Oliveira, Érica Maria ; dos Santos Vasconcelos, Crhisllane Rafaele ; Rezende, Antônio Mauro ; de Souza Lopes, Ana Catarina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1970-ce1de4c440310917ee06dbe277670050444df9944f62b0af734b870e103bc2203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aminoglycosides</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Carbapenems</topic><topic>Cell Biology</topic><topic>Ecology</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Horizontal transfer</topic><topic>Klebsiella</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Multidrug resistance</topic><topic>Nucleotide sequence</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Quinolones</topic><topic>Septicemia</topic><topic>Short Communication</topic><topic>Virulence</topic><topic>β-Lactam antibiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira Alves, Weverton</creatorcontrib><creatorcontrib>Scavuzzi, Alexsandra Maria Lima</creatorcontrib><creatorcontrib>Beltrão, Elizabeth Maria Bispo</creatorcontrib><creatorcontrib>de Oliveira, Érica Maria</creatorcontrib><creatorcontrib>dos Santos Vasconcelos, Crhisllane Rafaele</creatorcontrib><creatorcontrib>Rezende, Antônio Mauro</creatorcontrib><creatorcontrib>de Souza Lopes, Ana Catarina</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science 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pneumoniae with different plasmids in Brazil</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><date>2022-08-01</date><risdate>2022</risdate><volume>204</volume><issue>8</issue><spage>459</spage><epage>459</epage><pages>459-459</pages><artnum>459</artnum><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>To characterize phenotypically and genotypically an isolate of multidrug-resistant (MDR)
K. pneumoniae
from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated using PCR and sequencing the amplicons, and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all
β
-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variants
bla
NDM
-7
associated with
bla
KPC-2
and the
cps
,
wabG
,
fim-H
,
mrkD
and
entB
virulence genes were identified. The analysis of plasmid revealed the presence of
bla
CTX-M15
,
aac(3)-IVa
,
aph(3′)-Ia
,
aph(4)-Ia
,
aac(6′)ib-cr
,
mph(A)
and
catB3
, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the
bla
NDM
-7
gene in Recife-PE and we suggest that this variant is located in IncX3
.
These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00203-022-03051-0</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-7821-1068</orcidid><orcidid>https://orcid.org/0000-0003-4195-4380</orcidid><orcidid>https://orcid.org/0000-0002-9650-7900</orcidid><orcidid>https://orcid.org/0000-0001-8290-5775</orcidid><orcidid>https://orcid.org/0000-0003-4775-1779</orcidid><orcidid>https://orcid.org/0000-0003-0277-108X</orcidid><orcidid>https://orcid.org/0000-0001-8780-3460</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-8933 |
| ispartof | Archives of microbiology, 2022-08, Vol.204 (8), p.459-459, Article 459 |
| issn | 0302-8933 1432-072X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2685032854 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Aminoglycosides Biochemistry Biomedical and Life Sciences Biotechnology Carbapenems Cell Biology Ecology Gene sequencing Genes Horizontal transfer Klebsiella Life Sciences Microbial Ecology Microbiology Multidrug resistance Nucleotide sequence Plasmids Polymerase chain reaction Quinolones Septicemia Short Communication Virulence β-Lactam antibiotics |
| title | Occurrence of blaNDM-7 and association with blaKPC-2, blaCTX-M15, aac, aph, mph(A), catB3 and virulence genes in a clinical isolate of Klebsiella pneumoniae with different plasmids in Brazil |
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