Effect of cell density on the malignant biological behavior of breast cancer by altering the subcellular localization of ANXA2 and its clinical implications
Objective To investigate the subcellular localization of ANXA2 in breast cancer of different cell densities in humans and its relationship with the clinicopathological features of patients. To investigate the differences in ANXA2 subcellular localization in MDA-MB-231 cells of different cell densiti...
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| Veröffentlicht in: | Clinical & translational oncology 2022-11, Vol.24 (11), p.2136-2145 |
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| container_title | Clinical & translational oncology |
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| creator | Chen, Jingtai Su, Xiaohan Tan, Qiao Pu, Hongyu Zhang, Linxing Kang, Yuqing Tang, Yunhui Zhao, Xiaobo Hou, Wei Qian, Shuangqiang Deng, Shishan Hou, Lingmi Gao, Yanchun |
| description | Objective
To investigate the subcellular localization of ANXA2 in breast cancer of different cell densities in humans and its relationship with the clinicopathological features of patients. To investigate the differences in ANXA2 subcellular localization in MDA-MB-231 cells of different cell densities. To compare the proliferation, invasion, and migration ability of MDA-MB-231 cells under different ANXA2 subcellular localization.
Methods
Immunohistochemistry was applied to detect the subcellular localization of ANXA2 in tissue sections of 60 breast cancer patients, and the association with ANXA2 subcellular localization was verified in conjunction with cell density. To investigate the relationship between cell density and clinicopathological data of breast cancer patients. To establish high- and low-density models of MDA-MB-231 breast cancer cell lines and verify the subcellular localization of ANXA2 using immunofluorescence and observation under confocal microscopy. The proliferation, migration, and invasion ability of MDA-MB-231 cells under different subcellular localization of ANXA2 were detected and compared using CCK-8 assay and Transwell assay. After changing the subcellular localization of ANXA2 in high-density MDA-MB-231 cells with PY-60, changes in biological behaviors of the compared MDA-MB-231 cells were observed. Two different 4T1 cell lines with high and low densities were implanted subcutaneously in nude mice to observe the effects of different cell densities on tumor growth in nude mice.
Results
The clinical data showed that breast cancer with high cell density had higher T stage and higher TNM stage, and the cell density was positively correlated with breast cancer mass size. ANXA2 was mainly localized to the cell membrane when the cell density of breast cancer cells was high and to the cytoplasm when the cell density was low. The CCK-8 assay showed that the proliferation rate of MDA-MB-231 cells increased (
P
|
| doi_str_mv | 10.1007/s12094-022-02865-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2684095380</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2684095380</sourcerecordid><originalsourceid>FETCH-LOGICAL-c275t-c4d94a666bcb93de7b324fa72af764ab1843af29a9b42151339a395f8fe27a333</originalsourceid><addsrcrecordid>eNp9kTtPBCEUhSdGE59_wIrSZpTHvCg3xley0UYTO3JhYcWwsAJjsv4Wf6zMroWVxQ2nON_JvZyqOif4kmDcXyVCMW9qTGmZoWtrvFcdkY7zmuG23f-jD6vjlN5xUR0hR9X3jTFaZRQMUto5tNA-2bxBwaP8ptEKnF168BlJG1xYWgUOSf0GnzbECZJRQ8pIgVc6IrlB4LKO1i-3eBrllDo6iMiFwtovyLZkF3L2-DqjCPwC2ZyQctZvw-1q7YqYXOm0OjDgkj77fU-ql9ub5-v7ev5093A9m9eK9m2uVbPgDXRdJ5XkbKF7yWhjoKdg-q4BSYaGgaEcuGwoaQljHBhvzWA07YExdlJd7HLXMXyMOmWxsmlaHLwOYxK0GxrMWzbgYqU7q4ohpaiNWEe7grgRBIupCrGrQpQqxLYKMUFsB6X19DU6ivcwRl9O-o_6ARCQjuY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2684095380</pqid></control><display><type>article</type><title>Effect of cell density on the malignant biological behavior of breast cancer by altering the subcellular localization of ANXA2 and its clinical implications</title><source>SpringerLink (Online service)</source><creator>Chen, Jingtai ; Su, Xiaohan ; Tan, Qiao ; Pu, Hongyu ; Zhang, Linxing ; Kang, Yuqing ; Tang, Yunhui ; Zhao, Xiaobo ; Hou, Wei ; Qian, Shuangqiang ; Deng, Shishan ; Hou, Lingmi ; Gao, Yanchun</creator><creatorcontrib>Chen, Jingtai ; Su, Xiaohan ; Tan, Qiao ; Pu, Hongyu ; Zhang, Linxing ; Kang, Yuqing ; Tang, Yunhui ; Zhao, Xiaobo ; Hou, Wei ; Qian, Shuangqiang ; Deng, Shishan ; Hou, Lingmi ; Gao, Yanchun</creatorcontrib><description>Objective
To investigate the subcellular localization of ANXA2 in breast cancer of different cell densities in humans and its relationship with the clinicopathological features of patients. To investigate the differences in ANXA2 subcellular localization in MDA-MB-231 cells of different cell densities. To compare the proliferation, invasion, and migration ability of MDA-MB-231 cells under different ANXA2 subcellular localization.
Methods
Immunohistochemistry was applied to detect the subcellular localization of ANXA2 in tissue sections of 60 breast cancer patients, and the association with ANXA2 subcellular localization was verified in conjunction with cell density. To investigate the relationship between cell density and clinicopathological data of breast cancer patients. To establish high- and low-density models of MDA-MB-231 breast cancer cell lines and verify the subcellular localization of ANXA2 using immunofluorescence and observation under confocal microscopy. The proliferation, migration, and invasion ability of MDA-MB-231 cells under different subcellular localization of ANXA2 were detected and compared using CCK-8 assay and Transwell assay. After changing the subcellular localization of ANXA2 in high-density MDA-MB-231 cells with PY-60, changes in biological behaviors of the compared MDA-MB-231 cells were observed. Two different 4T1 cell lines with high and low densities were implanted subcutaneously in nude mice to observe the effects of different cell densities on tumor growth in nude mice.
Results
The clinical data showed that breast cancer with high cell density had higher T stage and higher TNM stage, and the cell density was positively correlated with breast cancer mass size. ANXA2 was mainly localized to the cell membrane when the cell density of breast cancer cells was high and to the cytoplasm when the cell density was low. The CCK-8 assay showed that the proliferation rate of MDA-MB-231 cells increased (
P
< 0.05) after shifting the subcellular localization of ANXA2 from the cell membrane to the cytoplasm. Transwell invasion assay and Transwell migration assay showed that the invasion and migration ability of MDA-MB-231 cells increased significantly after the subcellular localization of ANXA2 was transferred from the cell membrane to the cytoplasm (
P
< 0.05). The animal experiments showed that high-density breast cancer cells could promote the growth of subcutaneous tumors in nude mice relative to low-density breast cancer cells.
Conclusion
Cell density can regulate the subcellular localization of ANXA2, and changes in the subcellular localization of ANXA2 are accompanied by the changes in the biological behavior of breast cancer.</description><identifier>ISSN: 1699-3055</identifier><identifier>EISSN: 1699-3055</identifier><identifier>DOI: 10.1007/s12094-022-02865-0</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Medicine ; Medicine & Public Health ; Oncology ; Research Article</subject><ispartof>Clinical & translational oncology, 2022-11, Vol.24 (11), p.2136-2145</ispartof><rights>The Author(s), under exclusive licence to Federación de Sociedades Españolas de Oncología (FESEO) 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c275t-c4d94a666bcb93de7b324fa72af764ab1843af29a9b42151339a395f8fe27a333</cites><orcidid>0000-0003-2662-4551</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12094-022-02865-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12094-022-02865-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Chen, Jingtai</creatorcontrib><creatorcontrib>Su, Xiaohan</creatorcontrib><creatorcontrib>Tan, Qiao</creatorcontrib><creatorcontrib>Pu, Hongyu</creatorcontrib><creatorcontrib>Zhang, Linxing</creatorcontrib><creatorcontrib>Kang, Yuqing</creatorcontrib><creatorcontrib>Tang, Yunhui</creatorcontrib><creatorcontrib>Zhao, Xiaobo</creatorcontrib><creatorcontrib>Hou, Wei</creatorcontrib><creatorcontrib>Qian, Shuangqiang</creatorcontrib><creatorcontrib>Deng, Shishan</creatorcontrib><creatorcontrib>Hou, Lingmi</creatorcontrib><creatorcontrib>Gao, Yanchun</creatorcontrib><title>Effect of cell density on the malignant biological behavior of breast cancer by altering the subcellular localization of ANXA2 and its clinical implications</title><title>Clinical & translational oncology</title><addtitle>Clin Transl Oncol</addtitle><description>Objective
To investigate the subcellular localization of ANXA2 in breast cancer of different cell densities in humans and its relationship with the clinicopathological features of patients. To investigate the differences in ANXA2 subcellular localization in MDA-MB-231 cells of different cell densities. To compare the proliferation, invasion, and migration ability of MDA-MB-231 cells under different ANXA2 subcellular localization.
Methods
Immunohistochemistry was applied to detect the subcellular localization of ANXA2 in tissue sections of 60 breast cancer patients, and the association with ANXA2 subcellular localization was verified in conjunction with cell density. To investigate the relationship between cell density and clinicopathological data of breast cancer patients. To establish high- and low-density models of MDA-MB-231 breast cancer cell lines and verify the subcellular localization of ANXA2 using immunofluorescence and observation under confocal microscopy. The proliferation, migration, and invasion ability of MDA-MB-231 cells under different subcellular localization of ANXA2 were detected and compared using CCK-8 assay and Transwell assay. After changing the subcellular localization of ANXA2 in high-density MDA-MB-231 cells with PY-60, changes in biological behaviors of the compared MDA-MB-231 cells were observed. Two different 4T1 cell lines with high and low densities were implanted subcutaneously in nude mice to observe the effects of different cell densities on tumor growth in nude mice.
Results
The clinical data showed that breast cancer with high cell density had higher T stage and higher TNM stage, and the cell density was positively correlated with breast cancer mass size. ANXA2 was mainly localized to the cell membrane when the cell density of breast cancer cells was high and to the cytoplasm when the cell density was low. The CCK-8 assay showed that the proliferation rate of MDA-MB-231 cells increased (
P
< 0.05) after shifting the subcellular localization of ANXA2 from the cell membrane to the cytoplasm. Transwell invasion assay and Transwell migration assay showed that the invasion and migration ability of MDA-MB-231 cells increased significantly after the subcellular localization of ANXA2 was transferred from the cell membrane to the cytoplasm (
P
< 0.05). The animal experiments showed that high-density breast cancer cells could promote the growth of subcutaneous tumors in nude mice relative to low-density breast cancer cells.
Conclusion
Cell density can regulate the subcellular localization of ANXA2, and changes in the subcellular localization of ANXA2 are accompanied by the changes in the biological behavior of breast cancer.</description><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Oncology</subject><subject>Research Article</subject><issn>1699-3055</issn><issn>1699-3055</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kTtPBCEUhSdGE59_wIrSZpTHvCg3xley0UYTO3JhYcWwsAJjsv4Wf6zMroWVxQ2nON_JvZyqOif4kmDcXyVCMW9qTGmZoWtrvFcdkY7zmuG23f-jD6vjlN5xUR0hR9X3jTFaZRQMUto5tNA-2bxBwaP8ptEKnF168BlJG1xYWgUOSf0GnzbECZJRQ8pIgVc6IrlB4LKO1i-3eBrllDo6iMiFwtovyLZkF3L2-DqjCPwC2ZyQctZvw-1q7YqYXOm0OjDgkj77fU-ql9ub5-v7ev5093A9m9eK9m2uVbPgDXRdJ5XkbKF7yWhjoKdg-q4BSYaGgaEcuGwoaQljHBhvzWA07YExdlJd7HLXMXyMOmWxsmlaHLwOYxK0GxrMWzbgYqU7q4ohpaiNWEe7grgRBIupCrGrQpQqxLYKMUFsB6X19DU6ivcwRl9O-o_6ARCQjuY</recordid><startdate>20221101</startdate><enddate>20221101</enddate><creator>Chen, Jingtai</creator><creator>Su, Xiaohan</creator><creator>Tan, Qiao</creator><creator>Pu, Hongyu</creator><creator>Zhang, Linxing</creator><creator>Kang, Yuqing</creator><creator>Tang, Yunhui</creator><creator>Zhao, Xiaobo</creator><creator>Hou, Wei</creator><creator>Qian, Shuangqiang</creator><creator>Deng, Shishan</creator><creator>Hou, Lingmi</creator><creator>Gao, Yanchun</creator><general>Springer International Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2662-4551</orcidid></search><sort><creationdate>20221101</creationdate><title>Effect of cell density on the malignant biological behavior of breast cancer by altering the subcellular localization of ANXA2 and its clinical implications</title><author>Chen, Jingtai ; Su, Xiaohan ; Tan, Qiao ; Pu, Hongyu ; Zhang, Linxing ; Kang, Yuqing ; Tang, Yunhui ; Zhao, Xiaobo ; Hou, Wei ; Qian, Shuangqiang ; Deng, Shishan ; Hou, Lingmi ; Gao, Yanchun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-c4d94a666bcb93de7b324fa72af764ab1843af29a9b42151339a395f8fe27a333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Oncology</topic><topic>Research Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Jingtai</creatorcontrib><creatorcontrib>Su, Xiaohan</creatorcontrib><creatorcontrib>Tan, Qiao</creatorcontrib><creatorcontrib>Pu, Hongyu</creatorcontrib><creatorcontrib>Zhang, Linxing</creatorcontrib><creatorcontrib>Kang, Yuqing</creatorcontrib><creatorcontrib>Tang, Yunhui</creatorcontrib><creatorcontrib>Zhao, Xiaobo</creatorcontrib><creatorcontrib>Hou, Wei</creatorcontrib><creatorcontrib>Qian, Shuangqiang</creatorcontrib><creatorcontrib>Deng, Shishan</creatorcontrib><creatorcontrib>Hou, Lingmi</creatorcontrib><creatorcontrib>Gao, Yanchun</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical & translational oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Jingtai</au><au>Su, Xiaohan</au><au>Tan, Qiao</au><au>Pu, Hongyu</au><au>Zhang, Linxing</au><au>Kang, Yuqing</au><au>Tang, Yunhui</au><au>Zhao, Xiaobo</au><au>Hou, Wei</au><au>Qian, Shuangqiang</au><au>Deng, Shishan</au><au>Hou, Lingmi</au><au>Gao, Yanchun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of cell density on the malignant biological behavior of breast cancer by altering the subcellular localization of ANXA2 and its clinical implications</atitle><jtitle>Clinical & translational oncology</jtitle><stitle>Clin Transl Oncol</stitle><date>2022-11-01</date><risdate>2022</risdate><volume>24</volume><issue>11</issue><spage>2136</spage><epage>2145</epage><pages>2136-2145</pages><issn>1699-3055</issn><eissn>1699-3055</eissn><abstract>Objective
To investigate the subcellular localization of ANXA2 in breast cancer of different cell densities in humans and its relationship with the clinicopathological features of patients. To investigate the differences in ANXA2 subcellular localization in MDA-MB-231 cells of different cell densities. To compare the proliferation, invasion, and migration ability of MDA-MB-231 cells under different ANXA2 subcellular localization.
Methods
Immunohistochemistry was applied to detect the subcellular localization of ANXA2 in tissue sections of 60 breast cancer patients, and the association with ANXA2 subcellular localization was verified in conjunction with cell density. To investigate the relationship between cell density and clinicopathological data of breast cancer patients. To establish high- and low-density models of MDA-MB-231 breast cancer cell lines and verify the subcellular localization of ANXA2 using immunofluorescence and observation under confocal microscopy. The proliferation, migration, and invasion ability of MDA-MB-231 cells under different subcellular localization of ANXA2 were detected and compared using CCK-8 assay and Transwell assay. After changing the subcellular localization of ANXA2 in high-density MDA-MB-231 cells with PY-60, changes in biological behaviors of the compared MDA-MB-231 cells were observed. Two different 4T1 cell lines with high and low densities were implanted subcutaneously in nude mice to observe the effects of different cell densities on tumor growth in nude mice.
Results
The clinical data showed that breast cancer with high cell density had higher T stage and higher TNM stage, and the cell density was positively correlated with breast cancer mass size. ANXA2 was mainly localized to the cell membrane when the cell density of breast cancer cells was high and to the cytoplasm when the cell density was low. The CCK-8 assay showed that the proliferation rate of MDA-MB-231 cells increased (
P
< 0.05) after shifting the subcellular localization of ANXA2 from the cell membrane to the cytoplasm. Transwell invasion assay and Transwell migration assay showed that the invasion and migration ability of MDA-MB-231 cells increased significantly after the subcellular localization of ANXA2 was transferred from the cell membrane to the cytoplasm (
P
< 0.05). The animal experiments showed that high-density breast cancer cells could promote the growth of subcutaneous tumors in nude mice relative to low-density breast cancer cells.
Conclusion
Cell density can regulate the subcellular localization of ANXA2, and changes in the subcellular localization of ANXA2 are accompanied by the changes in the biological behavior of breast cancer.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s12094-022-02865-0</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-2662-4551</orcidid></addata></record> |
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| subjects | Medicine Medicine & Public Health Oncology Research Article |
| title | Effect of cell density on the malignant biological behavior of breast cancer by altering the subcellular localization of ANXA2 and its clinical implications |
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