Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library
Objectives Ovarian cancer is one of the most fatal gynecological malignancies. It is emergently needed to select a novel molecular fragment as a targeting element for the future development of molecular imaging diagnosis and targeting chemotherapy to ovarian cancer. Results After five rounds of biop...
Gespeichert in:
| Veröffentlicht in: | Biotechnology letters 2022-08, Vol.44 (8), p.951-960 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 960 |
|---|---|
| container_issue | 8 |
| container_start_page | 951 |
| container_title | Biotechnology letters |
| container_volume | 44 |
| creator | Gao, Qian Chen, Lirong Jia, Chenshuang Yuan, Yue Li, Xinyao Lu, Zheng Feng, Yang Zhao, Ruixia Zhao, Xuewei Wang, Yiwen Cheng, Sinan Zhang, Caixia Xu, Jie Shu, Zhan Duan, Wei Nie, Guochao Xiao, Li Hou, Yingchun |
| description | Objectives
Ovarian cancer is one of the most fatal gynecological malignancies. It is emergently needed to select a novel molecular fragment as a targeting element for the future development of molecular imaging diagnosis and targeting chemotherapy to ovarian cancer.
Results
After five rounds of biopanning, a total of 44 positive phage clones were selected from final phage displayed peptide library. Nine consensus sequences were found based on the assay of sequencing results, then one clone of each consensus group was characterized and identified further by immunofluorescence assay. The result showed the phage clone R20 presents best targeting capacity. Then we synthesized peptide (OSP2) clone R20 displayed, it was characterized with high specificity and sensitivity binding to human ovarian cancer by a tissue chip assay. The target of OSP2 was predicted and docked as human carbonic anhydrase XII (CA12), an important protein usually deregulated in cancer.
Conclusions
Taken together, OSP2 and its target indicate a novel investigation way in future to develop novel agent or drug delivery formulation for molecular imaging diagnosis and targeting chemotherapy of ovarian cancer. |
| doi_str_mv | 10.1007/s10529-022-03263-w |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2682784543</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2682784543</sourcerecordid><originalsourceid>FETCH-LOGICAL-c303t-57fb7d7703926e14647f5daec1f4999c684bda29a66b80d700a1d17c1bd7e4d33</originalsourceid><addsrcrecordid>eNp9kU2LFDEQhoO44Li7f8BTwIuXaOWjk8lRFl2FBQ_qOaST6jFLT9ImPS7z7zezIwoePBUUz_NSxUvIKw5vOYB51zgMwjIQgoEUWrKHZ2TDByOZNkY_JxvgirNBWfGCvGztHgCsAbMhx684Y1hTydTnSFPEvKYpBf-0KhP1tC0YTiu64LJ2gI4px5R3dC20_PI1-UyDzwErDTjPjU617Lu3_PA7ZDG1ZfZHjH_0OY3V1-MVuZj83PD697wk3z9--Hbzid19uf188_6OBQlyZYOZRhONAWmFRq60MtMQPQY-KWtt0Fs1Ri-s13rcQjQAnkduAh-jQRWlvCRvzrlLLT8P2Fa3T-10qM9YDs0JvRVmqwZ1Ql__g96XQ839uk5ZK4SQg-2UOFOhltYqTm6pad8_chzcqQ13bsP1NtxTG-6hS_IstQ7nHda_0f-xHgHH2o7F</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2699222359</pqid></control><display><type>article</type><title>Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library</title><source>SpringerLink Journals - AutoHoldings</source><creator>Gao, Qian ; Chen, Lirong ; Jia, Chenshuang ; Yuan, Yue ; Li, Xinyao ; Lu, Zheng ; Feng, Yang ; Zhao, Ruixia ; Zhao, Xuewei ; Wang, Yiwen ; Cheng, Sinan ; Zhang, Caixia ; Xu, Jie ; Shu, Zhan ; Duan, Wei ; Nie, Guochao ; Xiao, Li ; Hou, Yingchun</creator><creatorcontrib>Gao, Qian ; Chen, Lirong ; Jia, Chenshuang ; Yuan, Yue ; Li, Xinyao ; Lu, Zheng ; Feng, Yang ; Zhao, Ruixia ; Zhao, Xuewei ; Wang, Yiwen ; Cheng, Sinan ; Zhang, Caixia ; Xu, Jie ; Shu, Zhan ; Duan, Wei ; Nie, Guochao ; Xiao, Li ; Hou, Yingchun</creatorcontrib><description>Objectives
Ovarian cancer is one of the most fatal gynecological malignancies. It is emergently needed to select a novel molecular fragment as a targeting element for the future development of molecular imaging diagnosis and targeting chemotherapy to ovarian cancer.
Results
After five rounds of biopanning, a total of 44 positive phage clones were selected from final phage displayed peptide library. Nine consensus sequences were found based on the assay of sequencing results, then one clone of each consensus group was characterized and identified further by immunofluorescence assay. The result showed the phage clone R20 presents best targeting capacity. Then we synthesized peptide (OSP2) clone R20 displayed, it was characterized with high specificity and sensitivity binding to human ovarian cancer by a tissue chip assay. The target of OSP2 was predicted and docked as human carbonic anhydrase XII (CA12), an important protein usually deregulated in cancer.
Conclusions
Taken together, OSP2 and its target indicate a novel investigation way in future to develop novel agent or drug delivery formulation for molecular imaging diagnosis and targeting chemotherapy of ovarian cancer.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-022-03263-w</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Applied Microbiology ; Assaying ; Binding ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cancer ; Carbonic anhydrase ; Carbonic anhydrases ; Chemotherapy ; Deregulation ; Diagnosis ; Drug delivery ; Immunofluorescence ; Libraries ; Life Sciences ; Medical imaging ; Microbiology ; Original Research Paper ; Ovarian cancer ; Peptides ; Phages</subject><ispartof>Biotechnology letters, 2022-08, Vol.44 (8), p.951-960</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c303t-57fb7d7703926e14647f5daec1f4999c684bda29a66b80d700a1d17c1bd7e4d33</cites><orcidid>0000-0002-6659-3465</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-022-03263-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-022-03263-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Gao, Qian</creatorcontrib><creatorcontrib>Chen, Lirong</creatorcontrib><creatorcontrib>Jia, Chenshuang</creatorcontrib><creatorcontrib>Yuan, Yue</creatorcontrib><creatorcontrib>Li, Xinyao</creatorcontrib><creatorcontrib>Lu, Zheng</creatorcontrib><creatorcontrib>Feng, Yang</creatorcontrib><creatorcontrib>Zhao, Ruixia</creatorcontrib><creatorcontrib>Zhao, Xuewei</creatorcontrib><creatorcontrib>Wang, Yiwen</creatorcontrib><creatorcontrib>Cheng, Sinan</creatorcontrib><creatorcontrib>Zhang, Caixia</creatorcontrib><creatorcontrib>Xu, Jie</creatorcontrib><creatorcontrib>Shu, Zhan</creatorcontrib><creatorcontrib>Duan, Wei</creatorcontrib><creatorcontrib>Nie, Guochao</creatorcontrib><creatorcontrib>Xiao, Li</creatorcontrib><creatorcontrib>Hou, Yingchun</creatorcontrib><title>Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>Objectives
Ovarian cancer is one of the most fatal gynecological malignancies. It is emergently needed to select a novel molecular fragment as a targeting element for the future development of molecular imaging diagnosis and targeting chemotherapy to ovarian cancer.
Results
After five rounds of biopanning, a total of 44 positive phage clones were selected from final phage displayed peptide library. Nine consensus sequences were found based on the assay of sequencing results, then one clone of each consensus group was characterized and identified further by immunofluorescence assay. The result showed the phage clone R20 presents best targeting capacity. Then we synthesized peptide (OSP2) clone R20 displayed, it was characterized with high specificity and sensitivity binding to human ovarian cancer by a tissue chip assay. The target of OSP2 was predicted and docked as human carbonic anhydrase XII (CA12), an important protein usually deregulated in cancer.
Conclusions
Taken together, OSP2 and its target indicate a novel investigation way in future to develop novel agent or drug delivery formulation for molecular imaging diagnosis and targeting chemotherapy of ovarian cancer.</description><subject>Applied Microbiology</subject><subject>Assaying</subject><subject>Binding</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Carbonic anhydrase</subject><subject>Carbonic anhydrases</subject><subject>Chemotherapy</subject><subject>Deregulation</subject><subject>Diagnosis</subject><subject>Drug delivery</subject><subject>Immunofluorescence</subject><subject>Libraries</subject><subject>Life Sciences</subject><subject>Medical imaging</subject><subject>Microbiology</subject><subject>Original Research Paper</subject><subject>Ovarian cancer</subject><subject>Peptides</subject><subject>Phages</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU2LFDEQhoO44Li7f8BTwIuXaOWjk8lRFl2FBQ_qOaST6jFLT9ImPS7z7zezIwoePBUUz_NSxUvIKw5vOYB51zgMwjIQgoEUWrKHZ2TDByOZNkY_JxvgirNBWfGCvGztHgCsAbMhx684Y1hTydTnSFPEvKYpBf-0KhP1tC0YTiu64LJ2gI4px5R3dC20_PI1-UyDzwErDTjPjU617Lu3_PA7ZDG1ZfZHjH_0OY3V1-MVuZj83PD697wk3z9--Hbzid19uf188_6OBQlyZYOZRhONAWmFRq60MtMQPQY-KWtt0Fs1Ri-s13rcQjQAnkduAh-jQRWlvCRvzrlLLT8P2Fa3T-10qM9YDs0JvRVmqwZ1Ql__g96XQ839uk5ZK4SQg-2UOFOhltYqTm6pad8_chzcqQ13bsP1NtxTG-6hS_IstQ7nHda_0f-xHgHH2o7F</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>Gao, Qian</creator><creator>Chen, Lirong</creator><creator>Jia, Chenshuang</creator><creator>Yuan, Yue</creator><creator>Li, Xinyao</creator><creator>Lu, Zheng</creator><creator>Feng, Yang</creator><creator>Zhao, Ruixia</creator><creator>Zhao, Xuewei</creator><creator>Wang, Yiwen</creator><creator>Cheng, Sinan</creator><creator>Zhang, Caixia</creator><creator>Xu, Jie</creator><creator>Shu, Zhan</creator><creator>Duan, Wei</creator><creator>Nie, Guochao</creator><creator>Xiao, Li</creator><creator>Hou, Yingchun</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6659-3465</orcidid></search><sort><creationdate>20220801</creationdate><title>Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library</title><author>Gao, Qian ; Chen, Lirong ; Jia, Chenshuang ; Yuan, Yue ; Li, Xinyao ; Lu, Zheng ; Feng, Yang ; Zhao, Ruixia ; Zhao, Xuewei ; Wang, Yiwen ; Cheng, Sinan ; Zhang, Caixia ; Xu, Jie ; Shu, Zhan ; Duan, Wei ; Nie, Guochao ; Xiao, Li ; Hou, Yingchun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-57fb7d7703926e14647f5daec1f4999c684bda29a66b80d700a1d17c1bd7e4d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Applied Microbiology</topic><topic>Assaying</topic><topic>Binding</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Carbonic anhydrase</topic><topic>Carbonic anhydrases</topic><topic>Chemotherapy</topic><topic>Deregulation</topic><topic>Diagnosis</topic><topic>Drug delivery</topic><topic>Immunofluorescence</topic><topic>Libraries</topic><topic>Life Sciences</topic><topic>Medical imaging</topic><topic>Microbiology</topic><topic>Original Research Paper</topic><topic>Ovarian cancer</topic><topic>Peptides</topic><topic>Phages</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Qian</creatorcontrib><creatorcontrib>Chen, Lirong</creatorcontrib><creatorcontrib>Jia, Chenshuang</creatorcontrib><creatorcontrib>Yuan, Yue</creatorcontrib><creatorcontrib>Li, Xinyao</creatorcontrib><creatorcontrib>Lu, Zheng</creatorcontrib><creatorcontrib>Feng, Yang</creatorcontrib><creatorcontrib>Zhao, Ruixia</creatorcontrib><creatorcontrib>Zhao, Xuewei</creatorcontrib><creatorcontrib>Wang, Yiwen</creatorcontrib><creatorcontrib>Cheng, Sinan</creatorcontrib><creatorcontrib>Zhang, Caixia</creatorcontrib><creatorcontrib>Xu, Jie</creatorcontrib><creatorcontrib>Shu, Zhan</creatorcontrib><creatorcontrib>Duan, Wei</creatorcontrib><creatorcontrib>Nie, Guochao</creatorcontrib><creatorcontrib>Xiao, Li</creatorcontrib><creatorcontrib>Hou, Yingchun</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Qian</au><au>Chen, Lirong</au><au>Jia, Chenshuang</au><au>Yuan, Yue</au><au>Li, Xinyao</au><au>Lu, Zheng</au><au>Feng, Yang</au><au>Zhao, Ruixia</au><au>Zhao, Xuewei</au><au>Wang, Yiwen</au><au>Cheng, Sinan</au><au>Zhang, Caixia</au><au>Xu, Jie</au><au>Shu, Zhan</au><au>Duan, Wei</au><au>Nie, Guochao</au><au>Xiao, Li</au><au>Hou, Yingchun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><date>2022-08-01</date><risdate>2022</risdate><volume>44</volume><issue>8</issue><spage>951</spage><epage>960</epage><pages>951-960</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>Objectives
Ovarian cancer is one of the most fatal gynecological malignancies. It is emergently needed to select a novel molecular fragment as a targeting element for the future development of molecular imaging diagnosis and targeting chemotherapy to ovarian cancer.
Results
After five rounds of biopanning, a total of 44 positive phage clones were selected from final phage displayed peptide library. Nine consensus sequences were found based on the assay of sequencing results, then one clone of each consensus group was characterized and identified further by immunofluorescence assay. The result showed the phage clone R20 presents best targeting capacity. Then we synthesized peptide (OSP2) clone R20 displayed, it was characterized with high specificity and sensitivity binding to human ovarian cancer by a tissue chip assay. The target of OSP2 was predicted and docked as human carbonic anhydrase XII (CA12), an important protein usually deregulated in cancer.
Conclusions
Taken together, OSP2 and its target indicate a novel investigation way in future to develop novel agent or drug delivery formulation for molecular imaging diagnosis and targeting chemotherapy of ovarian cancer.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10529-022-03263-w</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-6659-3465</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2022-08, Vol.44 (8), p.951-960 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2682784543 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Applied Microbiology Assaying Binding Biochemistry Biomedical and Life Sciences Biotechnology Cancer Carbonic anhydrase Carbonic anhydrases Chemotherapy Deregulation Diagnosis Drug delivery Immunofluorescence Libraries Life Sciences Medical imaging Microbiology Original Research Paper Ovarian cancer Peptides Phages |
| title | Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T19%3A18%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Selection%20and%20identification%20of%20a%20specific%20peptide%20binding%20to%20ovarian%20cancer%20cells%20from%20a%20phage-displayed%20peptide%20library&rft.jtitle=Biotechnology%20letters&rft.au=Gao,%20Qian&rft.date=2022-08-01&rft.volume=44&rft.issue=8&rft.spage=951&rft.epage=960&rft.pages=951-960&rft.issn=0141-5492&rft.eissn=1573-6776&rft_id=info:doi/10.1007/s10529-022-03263-w&rft_dat=%3Cproquest_cross%3E2682784543%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2699222359&rft_id=info:pmid/&rfr_iscdi=true |