Photoactivatable CRISPR/Cas12a Strategy for One-Pot DETECTR Molecular Diagnosis

As a golden partner of recombinase polymerase amplification (RPA), CRISPR/Cas12a has been proven to solve the false-positive problem caused by nonspecific amplification perfectly; meanwhile, its trans-cleave activity has further enhanced the sensitivity. However, the solution transfer operation afte...

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Veröffentlicht in:	Analytical chemistry (Washington) 2022-07, Vol.94 (27), p.9724-9731
Hauptverfasser:	Chen, Yong, Xu, Xiaoling, Wang, Jiachun, Zhang, Yibin, Zeng, Wentao, Liu, Yizhen, Zhang, Xueji
Format:	Artikel
Sprache:	eng
Schlagworte:	Air pollution Amplification Analytical chemistry Chemistry Contamination CRISPR Nucleic acids Recombinase Sensitivity enhancement Strategy Ultraviolet radiation
Online-Zugang:	Volltext
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