Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos
The cold‐adapted bacterium Pseudomonas sp. ID1 produces the extracellular exopolysaccharide ID1 (EPS ID1) with cryoprotective activity. This study was designed to optimize the vitrification/in‐straw warming protocol of in vitro‐produced (IVP) blastocysts by adding EPS ID1 to the vitrification media....
Gespeichert in:
| Veröffentlicht in: | Reproduction in domestic animals 2022-10, Vol.57 (S5), p.53-57 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 57 |
|---|---|
| container_issue | S5 |
| container_start_page | 53 |
| container_title | Reproduction in domestic animals |
| container_volume | 57 |
| creator | Martínez‐Rodero, Iris Salas‐Huetos, Albert Ordóñez‐León, Alina Hidalgo, Carlos Olegario Yeste, Marc Mercadé, Elena Mogas, Teresa |
| description | The cold‐adapted bacterium Pseudomonas sp. ID1 produces the extracellular exopolysaccharide ID1 (EPS ID1) with cryoprotective activity. This study was designed to optimize the vitrification/in‐straw warming protocol of in vitro‐produced (IVP) blastocysts by adding EPS ID1 to the vitrification media. Day 7‐expanded blastocysts were vitrified/warmed using the VitTrans device after the addition of 0 or 100 μg/mL EPS ID1 to the vitrification media. Blastocysts vitrified by the Cryotop method and fresh non‐vitrified blastocysts served as controls. Outcomes were assessed in the warmed embryos in terms of survival rates and mRNA relative abundances of BAX, BCL2, GPX1, and CDX2 genes. No differences in survival rates were observed at 3 h post‐warming between vitrification treatments. At 24 h post‐warming, the addition of EPS prior to vitrification with the VitTrans device produced similar survival rates to Cryotop‐vitrified embryos and similar hatching rates to fresh non‐vitrified or Cryotop‐vitrified embryos. No differences emerged in BCL2 gene expression. Lower BAX (p |
| doi_str_mv | 10.1111/rda.14191 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2681035560</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2720293699</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3881-89d464fdbca0ea89d72e081e653be6dd1c80da6b929567150d0c7d827ab786653</originalsourceid><addsrcrecordid>eNp10cFO2zAYB3BrAo2O7cALTJa4sEOo7SS2c0TtBkhISAjOlmN_oa6SuLOTlnLaI-wZ9yRzKewwCV8syz_9_cl_hE4oOadpTYPV57SgFf2AJrTIq4yUOT1AE1LlPOOCyyP0KcYlIbSUQnxER3kpCslYPkHPs7D1q-AHMIPuBxx8C9g3GJ78yrfbqI1Z6OAs4Os5xa7HwwLw2g3BNc7owfl-6vo_v37HIegN3ujQuf5xF5Dojvl0l-LtaMDi2q9dDxi6Oj0aP6PDRrcRvrzux-jhx_f72VV2c3t5Pbu4yUwuJc1kZQteNLY2moBOJ8GASAq8zGvg1lIjidW8rlhVckFLYokRVjKhayF5UsfobJ-b5vg5QhxU56KBttU9-DEqxiUleVlykujpf3Tpx9Cn6RQTjLD0n1WV1Le9MsHHGKBRq-A6HbaKErUrRKVC1EshyX59TRzrDuw_-dZAAtM92LgWtu8nqbv5xT7yL-zNl_Y</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2720293699</pqid></control><display><type>article</type><title>Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Martínez‐Rodero, Iris ; Salas‐Huetos, Albert ; Ordóñez‐León, Alina ; Hidalgo, Carlos Olegario ; Yeste, Marc ; Mercadé, Elena ; Mogas, Teresa</creator><creatorcontrib>Martínez‐Rodero, Iris ; Salas‐Huetos, Albert ; Ordóñez‐León, Alina ; Hidalgo, Carlos Olegario ; Yeste, Marc ; Mercadé, Elena ; Mogas, Teresa</creatorcontrib><description>The cold‐adapted bacterium Pseudomonas sp. ID1 produces the extracellular exopolysaccharide ID1 (EPS ID1) with cryoprotective activity. This study was designed to optimize the vitrification/in‐straw warming protocol of in vitro‐produced (IVP) blastocysts by adding EPS ID1 to the vitrification media. Day 7‐expanded blastocysts were vitrified/warmed using the VitTrans device after the addition of 0 or 100 μg/mL EPS ID1 to the vitrification media. Blastocysts vitrified by the Cryotop method and fresh non‐vitrified blastocysts served as controls. Outcomes were assessed in the warmed embryos in terms of survival rates and mRNA relative abundances of BAX, BCL2, GPX1, and CDX2 genes. No differences in survival rates were observed at 3 h post‐warming between vitrification treatments. At 24 h post‐warming, the addition of EPS prior to vitrification with the VitTrans device produced similar survival rates to Cryotop‐vitrified embryos and similar hatching rates to fresh non‐vitrified or Cryotop‐vitrified embryos. No differences emerged in BCL2 gene expression. Lower BAX (p < .05) and higher GPX1 (p < .05) and CDX2 (p < .1) gene expression were observed in expanded and/or hatched blastocysts derived from VitTrans‐EPS‐vitrified embryos when compared to those from the non‐supplemented group. In conclusion, addition of EPS not only promoted blastocyst survival and hatching after VitTrans vitrification/warming but also modified the expression of genes associated with better embryo quality.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.14191</identifier><identifier>PMID: 35748223</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; apoptosis ; bcl-2-Associated X Protein - genetics ; Blastocyst ; Blastocysts ; Cattle ; CDX2 protein ; cell differentiation ; cryopreservation ; Cryopreservation - methods ; Cryopreservation - veterinary ; Cryoprotective Agents ; Cryoprotectors ; Embryos ; Exopolysaccharides ; Fertilization in Vitro - methods ; Fertilization in Vitro - veterinary ; Gene expression ; Genes ; Hatching ; Id1 protein ; oxidative stress ; RNA, Messenger ; Straw ; Survival ; survival rate ; Vitrification</subject><ispartof>Reproduction in domestic animals, 2022-10, Vol.57 (S5), p.53-57</ispartof><rights>2022 The Authors. published by Wiley‐VCH GmbH.</rights><rights>2022 The Authors. Reproduction in Domestic Animals published by Wiley-VCH GmbH.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3881-89d464fdbca0ea89d72e081e653be6dd1c80da6b929567150d0c7d827ab786653</citedby><cites>FETCH-LOGICAL-c3881-89d464fdbca0ea89d72e081e653be6dd1c80da6b929567150d0c7d827ab786653</cites><orcidid>0000-0002-9810-1517 ; 0000-0001-5914-6862 ; 0000-0002-2209-340X ; 0000-0001-7057-2045 ; 0000-0001-7828-2210 ; 0000-0002-6733-1328</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.14191$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.14191$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35748223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martínez‐Rodero, Iris</creatorcontrib><creatorcontrib>Salas‐Huetos, Albert</creatorcontrib><creatorcontrib>Ordóñez‐León, Alina</creatorcontrib><creatorcontrib>Hidalgo, Carlos Olegario</creatorcontrib><creatorcontrib>Yeste, Marc</creatorcontrib><creatorcontrib>Mercadé, Elena</creatorcontrib><creatorcontrib>Mogas, Teresa</creatorcontrib><title>Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>The cold‐adapted bacterium Pseudomonas sp. ID1 produces the extracellular exopolysaccharide ID1 (EPS ID1) with cryoprotective activity. This study was designed to optimize the vitrification/in‐straw warming protocol of in vitro‐produced (IVP) blastocysts by adding EPS ID1 to the vitrification media. Day 7‐expanded blastocysts were vitrified/warmed using the VitTrans device after the addition of 0 or 100 μg/mL EPS ID1 to the vitrification media. Blastocysts vitrified by the Cryotop method and fresh non‐vitrified blastocysts served as controls. Outcomes were assessed in the warmed embryos in terms of survival rates and mRNA relative abundances of BAX, BCL2, GPX1, and CDX2 genes. No differences in survival rates were observed at 3 h post‐warming between vitrification treatments. At 24 h post‐warming, the addition of EPS prior to vitrification with the VitTrans device produced similar survival rates to Cryotop‐vitrified embryos and similar hatching rates to fresh non‐vitrified or Cryotop‐vitrified embryos. No differences emerged in BCL2 gene expression. Lower BAX (p < .05) and higher GPX1 (p < .05) and CDX2 (p < .1) gene expression were observed in expanded and/or hatched blastocysts derived from VitTrans‐EPS‐vitrified embryos when compared to those from the non‐supplemented group. In conclusion, addition of EPS not only promoted blastocyst survival and hatching after VitTrans vitrification/warming but also modified the expression of genes associated with better embryo quality.</description><subject>Animals</subject><subject>apoptosis</subject><subject>bcl-2-Associated X Protein - genetics</subject><subject>Blastocyst</subject><subject>Blastocysts</subject><subject>Cattle</subject><subject>CDX2 protein</subject><subject>cell differentiation</subject><subject>cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents</subject><subject>Cryoprotectors</subject><subject>Embryos</subject><subject>Exopolysaccharides</subject><subject>Fertilization in Vitro - methods</subject><subject>Fertilization in Vitro - veterinary</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Hatching</subject><subject>Id1 protein</subject><subject>oxidative stress</subject><subject>RNA, Messenger</subject><subject>Straw</subject><subject>Survival</subject><subject>survival rate</subject><subject>Vitrification</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp10cFO2zAYB3BrAo2O7cALTJa4sEOo7SS2c0TtBkhISAjOlmN_oa6SuLOTlnLaI-wZ9yRzKewwCV8syz_9_cl_hE4oOadpTYPV57SgFf2AJrTIq4yUOT1AE1LlPOOCyyP0KcYlIbSUQnxER3kpCslYPkHPs7D1q-AHMIPuBxx8C9g3GJ78yrfbqI1Z6OAs4Os5xa7HwwLw2g3BNc7owfl-6vo_v37HIegN3ujQuf5xF5Dojvl0l-LtaMDi2q9dDxi6Oj0aP6PDRrcRvrzux-jhx_f72VV2c3t5Pbu4yUwuJc1kZQteNLY2moBOJ8GASAq8zGvg1lIjidW8rlhVckFLYokRVjKhayF5UsfobJ-b5vg5QhxU56KBttU9-DEqxiUleVlykujpf3Tpx9Cn6RQTjLD0n1WV1Le9MsHHGKBRq-A6HbaKErUrRKVC1EshyX59TRzrDuw_-dZAAtM92LgWtu8nqbv5xT7yL-zNl_Y</recordid><startdate>202210</startdate><enddate>202210</enddate><creator>Martínez‐Rodero, Iris</creator><creator>Salas‐Huetos, Albert</creator><creator>Ordóñez‐León, Alina</creator><creator>Hidalgo, Carlos Olegario</creator><creator>Yeste, Marc</creator><creator>Mercadé, Elena</creator><creator>Mogas, Teresa</creator><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9810-1517</orcidid><orcidid>https://orcid.org/0000-0001-5914-6862</orcidid><orcidid>https://orcid.org/0000-0002-2209-340X</orcidid><orcidid>https://orcid.org/0000-0001-7057-2045</orcidid><orcidid>https://orcid.org/0000-0001-7828-2210</orcidid><orcidid>https://orcid.org/0000-0002-6733-1328</orcidid></search><sort><creationdate>202210</creationdate><title>Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos</title><author>Martínez‐Rodero, Iris ; Salas‐Huetos, Albert ; Ordóñez‐León, Alina ; Hidalgo, Carlos Olegario ; Yeste, Marc ; Mercadé, Elena ; Mogas, Teresa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3881-89d464fdbca0ea89d72e081e653be6dd1c80da6b929567150d0c7d827ab786653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>apoptosis</topic><topic>bcl-2-Associated X Protein - genetics</topic><topic>Blastocyst</topic><topic>Blastocysts</topic><topic>Cattle</topic><topic>CDX2 protein</topic><topic>cell differentiation</topic><topic>cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents</topic><topic>Cryoprotectors</topic><topic>Embryos</topic><topic>Exopolysaccharides</topic><topic>Fertilization in Vitro - methods</topic><topic>Fertilization in Vitro - veterinary</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Hatching</topic><topic>Id1 protein</topic><topic>oxidative stress</topic><topic>RNA, Messenger</topic><topic>Straw</topic><topic>Survival</topic><topic>survival rate</topic><topic>Vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martínez‐Rodero, Iris</creatorcontrib><creatorcontrib>Salas‐Huetos, Albert</creatorcontrib><creatorcontrib>Ordóñez‐León, Alina</creatorcontrib><creatorcontrib>Hidalgo, Carlos Olegario</creatorcontrib><creatorcontrib>Yeste, Marc</creatorcontrib><creatorcontrib>Mercadé, Elena</creatorcontrib><creatorcontrib>Mogas, Teresa</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martínez‐Rodero, Iris</au><au>Salas‐Huetos, Albert</au><au>Ordóñez‐León, Alina</au><au>Hidalgo, Carlos Olegario</au><au>Yeste, Marc</au><au>Mercadé, Elena</au><au>Mogas, Teresa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2022-10</date><risdate>2022</risdate><volume>57</volume><issue>S5</issue><spage>53</spage><epage>57</epage><pages>53-57</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>The cold‐adapted bacterium Pseudomonas sp. ID1 produces the extracellular exopolysaccharide ID1 (EPS ID1) with cryoprotective activity. This study was designed to optimize the vitrification/in‐straw warming protocol of in vitro‐produced (IVP) blastocysts by adding EPS ID1 to the vitrification media. Day 7‐expanded blastocysts were vitrified/warmed using the VitTrans device after the addition of 0 or 100 μg/mL EPS ID1 to the vitrification media. Blastocysts vitrified by the Cryotop method and fresh non‐vitrified blastocysts served as controls. Outcomes were assessed in the warmed embryos in terms of survival rates and mRNA relative abundances of BAX, BCL2, GPX1, and CDX2 genes. No differences in survival rates were observed at 3 h post‐warming between vitrification treatments. At 24 h post‐warming, the addition of EPS prior to vitrification with the VitTrans device produced similar survival rates to Cryotop‐vitrified embryos and similar hatching rates to fresh non‐vitrified or Cryotop‐vitrified embryos. No differences emerged in BCL2 gene expression. Lower BAX (p < .05) and higher GPX1 (p < .05) and CDX2 (p < .1) gene expression were observed in expanded and/or hatched blastocysts derived from VitTrans‐EPS‐vitrified embryos when compared to those from the non‐supplemented group. In conclusion, addition of EPS not only promoted blastocyst survival and hatching after VitTrans vitrification/warming but also modified the expression of genes associated with better embryo quality.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>35748223</pmid><doi>10.1111/rda.14191</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-9810-1517</orcidid><orcidid>https://orcid.org/0000-0001-5914-6862</orcidid><orcidid>https://orcid.org/0000-0002-2209-340X</orcidid><orcidid>https://orcid.org/0000-0001-7057-2045</orcidid><orcidid>https://orcid.org/0000-0001-7828-2210</orcidid><orcidid>https://orcid.org/0000-0002-6733-1328</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0936-6768 |
| ispartof | Reproduction in domestic animals, 2022-10, Vol.57 (S5), p.53-57 |
| issn | 0936-6768 1439-0531 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2681035560 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals apoptosis bcl-2-Associated X Protein - genetics Blastocyst Blastocysts Cattle CDX2 protein cell differentiation cryopreservation Cryopreservation - methods Cryopreservation - veterinary Cryoprotective Agents Cryoprotectors Embryos Exopolysaccharides Fertilization in Vitro - methods Fertilization in Vitro - veterinary Gene expression Genes Hatching Id1 protein oxidative stress RNA, Messenger Straw Survival survival rate Vitrification |
| title | Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T19%3A44%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cryoprotectant%20role%20of%20exopolysaccharide%20ID1%20in%20the%20vitrification/in%E2%80%90straw%20warming%20of%20in%20vitro%E2%80%90produced%20bovine%20embryos&rft.jtitle=Reproduction%20in%20domestic%20animals&rft.au=Mart%C3%ADnez%E2%80%90Rodero,%20Iris&rft.date=2022-10&rft.volume=57&rft.issue=S5&rft.spage=53&rft.epage=57&rft.pages=53-57&rft.issn=0936-6768&rft.eissn=1439-0531&rft_id=info:doi/10.1111/rda.14191&rft_dat=%3Cproquest_cross%3E2720293699%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2720293699&rft_id=info:pmid/35748223&rfr_iscdi=true |