Genetic transformation of common beans (Phaseolus vulgaris L.) through Agrobacterium tumefaciens carrying Cry1Ab gene
Background Seed beetles are one of the most important causes of yield loss in bean production. It is essential to develop resistant varieties in the fight against these pests. Agrobacterium-based gene transformation is the most widely used breeding method worldwide to develop insect-resistant variet...
Gespeichert in:
| Veröffentlicht in: | Molecular biology reports 2022-07, Vol.49 (7), p.7195-7203 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7203 |
|---|---|
| container_issue | 7 |
| container_start_page | 7195 |
| container_title | Molecular biology reports |
| container_volume | 49 |
| creator | Sağlam Yılmaz, Sevil Khawar, Khalid Mahmood Çiftçi, Cemalettin Yaşar |
| description | Background
Seed beetles are one of the most important causes of yield loss in bean production. It is essential to develop resistant varieties in the fight against these pests. Agrobacterium-based gene transformation is the most widely used breeding method worldwide to develop insect-resistant varieties.
Methods and results
Embryonic axes and plumule explants were obtained from Agrobacterium tumefciens treated mature zygotic embryos of low and high raw protein-based common bean cultivars Akman 98 and Karacaşehir 90. Agrobacterium tumefaciens contained a synthetic
Bacillus thuringiensis
insecticidal crystal protein gene (Bt Cry1Ab) controlled by the 35S promoter and NOS terminator sequences. The transformation event was genotype and explant dependent. The plumule explants could not withstand kanamycin-based selection pressure and died. It was possible to get two transgenic plants using embryonic axis explants of low protein cultivar Akman 98. These results were validated using GUS analysis, PCR, RT-PCR, bioassay analysis, and ELISA test from the samples taken from T
0
and T
1
generations. Bioassay tests showed that these plants were protected from the damage of legume seed insects (
Bruchus
spp.).
Conclusions
The results are very encouraging and may help in producing better transgenic common bean germplasm leading to safe agriculture and reducing environmental pollutions. |
| doi_str_mv | 10.1007/s11033-022-07637-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2678420382</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2718274596</sourcerecordid><originalsourceid>FETCH-LOGICAL-c315t-56643d7ec195b3ee1f253c621df86cbc66683aa7fc6f7c573731457ba335d2663</originalsourceid><addsrcrecordid>eNqNkU9LAzEQxYMoWP98AU8BL_Wwmkk2yfZYilahoAc9h2yabFd2NzXZCP32RisIHsTTDMzvveHxELoAcg2EyJsIQBgrCKUFkYLJAg7QBLhkRTmT1SGaEEagKCsOx-gkxldCSAmST1Ba2sGOrcFj0EN0PvR6bP2AvcPG933eapsPePq00dH6LkX8nrpGhzbi1fUVHjfBp2aD503wtTajDW3q8Zh667RpbVYaHcKuHRq8CDuY17jJD8_QkdNdtOff8xS93N0-L-6L1ePyYTFfFYYBHwsuRMnW0hqY8ZpZC45yZgSFtauEqY0QomJaS2eEkyanlQxKLmvNGF9TIdgpmu59t8G_JRtH1bfR2K7Tg_UpKiqhorLks3-gQlYlJayiGb38hb76FIYcJFOVKOkM6CdF95QJPsZgndqGttdhp4Coz9LUvjSVS1NfpSnIIrYXxQwPjQ0_1n-oPgCLopmq</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2686429122</pqid></control><display><type>article</type><title>Genetic transformation of common beans (Phaseolus vulgaris L.) through Agrobacterium tumefaciens carrying Cry1Ab gene</title><source>Springer Nature - Complete Springer Journals</source><creator>Sağlam Yılmaz, Sevil ; Khawar, Khalid Mahmood ; Çiftçi, Cemalettin Yaşar</creator><creatorcontrib>Sağlam Yılmaz, Sevil ; Khawar, Khalid Mahmood ; Çiftçi, Cemalettin Yaşar</creatorcontrib><description>Background
Seed beetles are one of the most important causes of yield loss in bean production. It is essential to develop resistant varieties in the fight against these pests. Agrobacterium-based gene transformation is the most widely used breeding method worldwide to develop insect-resistant varieties.
Methods and results
Embryonic axes and plumule explants were obtained from Agrobacterium tumefciens treated mature zygotic embryos of low and high raw protein-based common bean cultivars Akman 98 and Karacaşehir 90. Agrobacterium tumefaciens contained a synthetic
Bacillus thuringiensis
insecticidal crystal protein gene (Bt Cry1Ab) controlled by the 35S promoter and NOS terminator sequences. The transformation event was genotype and explant dependent. The plumule explants could not withstand kanamycin-based selection pressure and died. It was possible to get two transgenic plants using embryonic axis explants of low protein cultivar Akman 98. These results were validated using GUS analysis, PCR, RT-PCR, bioassay analysis, and ELISA test from the samples taken from T
0
and T
1
generations. Bioassay tests showed that these plants were protected from the damage of legume seed insects (
Bruchus
spp.).
Conclusions
The results are very encouraging and may help in producing better transgenic common bean germplasm leading to safe agriculture and reducing environmental pollutions.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-022-07637-1</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agrobacterium ; Agrobacterium radiobacter ; Agrobacterium tumefaciens ; Animal Anatomy ; Animal Biochemistry ; Bacillus thuringiensis ; Beans ; Bioassays ; Biomedical and Life Sciences ; Bruchus ; Cry1ab gene ; Crystal proteins ; Cultivars ; Embryos ; Explants ; genes ; Genetic transformation ; genetically modified organisms ; genotype ; Genotypes ; Germplasm ; Histology ; insecticidal proteins ; Kanamycin ; Legumes ; Life Sciences ; Morphology ; Original Article ; Pest resistance ; Pests ; Phaseolus vulgaris ; Plant breeding ; plumule ; Progress in genomics ; promoter regions ; Proteins ; selection pressure ; transcriptomics and breeding of crops ; Transgenic plants</subject><ispartof>Molecular biology reports, 2022-07, Vol.49 (7), p.7195-7203</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-56643d7ec195b3ee1f253c621df86cbc66683aa7fc6f7c573731457ba335d2663</citedby><cites>FETCH-LOGICAL-c315t-56643d7ec195b3ee1f253c621df86cbc66683aa7fc6f7c573731457ba335d2663</cites><orcidid>0000-0003-1302-9147</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-022-07637-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-022-07637-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids></links><search><creatorcontrib>Sağlam Yılmaz, Sevil</creatorcontrib><creatorcontrib>Khawar, Khalid Mahmood</creatorcontrib><creatorcontrib>Çiftçi, Cemalettin Yaşar</creatorcontrib><title>Genetic transformation of common beans (Phaseolus vulgaris L.) through Agrobacterium tumefaciens carrying Cry1Ab gene</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><description>Background
Seed beetles are one of the most important causes of yield loss in bean production. It is essential to develop resistant varieties in the fight against these pests. Agrobacterium-based gene transformation is the most widely used breeding method worldwide to develop insect-resistant varieties.
Methods and results
Embryonic axes and plumule explants were obtained from Agrobacterium tumefciens treated mature zygotic embryos of low and high raw protein-based common bean cultivars Akman 98 and Karacaşehir 90. Agrobacterium tumefaciens contained a synthetic
Bacillus thuringiensis
insecticidal crystal protein gene (Bt Cry1Ab) controlled by the 35S promoter and NOS terminator sequences. The transformation event was genotype and explant dependent. The plumule explants could not withstand kanamycin-based selection pressure and died. It was possible to get two transgenic plants using embryonic axis explants of low protein cultivar Akman 98. These results were validated using GUS analysis, PCR, RT-PCR, bioassay analysis, and ELISA test from the samples taken from T
0
and T
1
generations. Bioassay tests showed that these plants were protected from the damage of legume seed insects (
Bruchus
spp.).
Conclusions
The results are very encouraging and may help in producing better transgenic common bean germplasm leading to safe agriculture and reducing environmental pollutions.</description><subject>Agrobacterium</subject><subject>Agrobacterium radiobacter</subject><subject>Agrobacterium tumefaciens</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Bacillus thuringiensis</subject><subject>Beans</subject><subject>Bioassays</subject><subject>Biomedical and Life Sciences</subject><subject>Bruchus</subject><subject>Cry1ab gene</subject><subject>Crystal proteins</subject><subject>Cultivars</subject><subject>Embryos</subject><subject>Explants</subject><subject>genes</subject><subject>Genetic transformation</subject><subject>genetically modified organisms</subject><subject>genotype</subject><subject>Genotypes</subject><subject>Germplasm</subject><subject>Histology</subject><subject>insecticidal proteins</subject><subject>Kanamycin</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Pest resistance</subject><subject>Pests</subject><subject>Phaseolus vulgaris</subject><subject>Plant breeding</subject><subject>plumule</subject><subject>Progress in genomics</subject><subject>promoter regions</subject><subject>Proteins</subject><subject>selection pressure</subject><subject>transcriptomics and breeding of crops</subject><subject>Transgenic plants</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU9LAzEQxYMoWP98AU8BL_Wwmkk2yfZYilahoAc9h2yabFd2NzXZCP32RisIHsTTDMzvveHxELoAcg2EyJsIQBgrCKUFkYLJAg7QBLhkRTmT1SGaEEagKCsOx-gkxldCSAmST1Ba2sGOrcFj0EN0PvR6bP2AvcPG933eapsPePq00dH6LkX8nrpGhzbi1fUVHjfBp2aD503wtTajDW3q8Zh667RpbVYaHcKuHRq8CDuY17jJD8_QkdNdtOff8xS93N0-L-6L1ePyYTFfFYYBHwsuRMnW0hqY8ZpZC45yZgSFtauEqY0QomJaS2eEkyanlQxKLmvNGF9TIdgpmu59t8G_JRtH1bfR2K7Tg_UpKiqhorLks3-gQlYlJayiGb38hb76FIYcJFOVKOkM6CdF95QJPsZgndqGttdhp4Coz9LUvjSVS1NfpSnIIrYXxQwPjQ0_1n-oPgCLopmq</recordid><startdate>20220701</startdate><enddate>20220701</enddate><creator>Sağlam Yılmaz, Sevil</creator><creator>Khawar, Khalid Mahmood</creator><creator>Çiftçi, Cemalettin Yaşar</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-1302-9147</orcidid></search><sort><creationdate>20220701</creationdate><title>Genetic transformation of common beans (Phaseolus vulgaris L.) through Agrobacterium tumefaciens carrying Cry1Ab gene</title><author>Sağlam Yılmaz, Sevil ; Khawar, Khalid Mahmood ; Çiftçi, Cemalettin Yaşar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-56643d7ec195b3ee1f253c621df86cbc66683aa7fc6f7c573731457ba335d2663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Agrobacterium</topic><topic>Agrobacterium radiobacter</topic><topic>Agrobacterium tumefaciens</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Bacillus thuringiensis</topic><topic>Beans</topic><topic>Bioassays</topic><topic>Biomedical and Life Sciences</topic><topic>Bruchus</topic><topic>Cry1ab gene</topic><topic>Crystal proteins</topic><topic>Cultivars</topic><topic>Embryos</topic><topic>Explants</topic><topic>genes</topic><topic>Genetic transformation</topic><topic>genetically modified organisms</topic><topic>genotype</topic><topic>Genotypes</topic><topic>Germplasm</topic><topic>Histology</topic><topic>insecticidal proteins</topic><topic>Kanamycin</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Pest resistance</topic><topic>Pests</topic><topic>Phaseolus vulgaris</topic><topic>Plant breeding</topic><topic>plumule</topic><topic>Progress in genomics</topic><topic>promoter regions</topic><topic>Proteins</topic><topic>selection pressure</topic><topic>transcriptomics and breeding of crops</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sağlam Yılmaz, Sevil</creatorcontrib><creatorcontrib>Khawar, Khalid Mahmood</creatorcontrib><creatorcontrib>Çiftçi, Cemalettin Yaşar</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sağlam Yılmaz, Sevil</au><au>Khawar, Khalid Mahmood</au><au>Çiftçi, Cemalettin Yaşar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic transformation of common beans (Phaseolus vulgaris L.) through Agrobacterium tumefaciens carrying Cry1Ab gene</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><date>2022-07-01</date><risdate>2022</risdate><volume>49</volume><issue>7</issue><spage>7195</spage><epage>7203</epage><pages>7195-7203</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Seed beetles are one of the most important causes of yield loss in bean production. It is essential to develop resistant varieties in the fight against these pests. Agrobacterium-based gene transformation is the most widely used breeding method worldwide to develop insect-resistant varieties.
Methods and results
Embryonic axes and plumule explants were obtained from Agrobacterium tumefciens treated mature zygotic embryos of low and high raw protein-based common bean cultivars Akman 98 and Karacaşehir 90. Agrobacterium tumefaciens contained a synthetic
Bacillus thuringiensis
insecticidal crystal protein gene (Bt Cry1Ab) controlled by the 35S promoter and NOS terminator sequences. The transformation event was genotype and explant dependent. The plumule explants could not withstand kanamycin-based selection pressure and died. It was possible to get two transgenic plants using embryonic axis explants of low protein cultivar Akman 98. These results were validated using GUS analysis, PCR, RT-PCR, bioassay analysis, and ELISA test from the samples taken from T
0
and T
1
generations. Bioassay tests showed that these plants were protected from the damage of legume seed insects (
Bruchus
spp.).
Conclusions
The results are very encouraging and may help in producing better transgenic common bean germplasm leading to safe agriculture and reducing environmental pollutions.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11033-022-07637-1</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-1302-9147</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2022-07, Vol.49 (7), p.7195-7203 |
| issn | 0301-4851 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2678420382 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Agrobacterium Agrobacterium radiobacter Agrobacterium tumefaciens Animal Anatomy Animal Biochemistry Bacillus thuringiensis Beans Bioassays Biomedical and Life Sciences Bruchus Cry1ab gene Crystal proteins Cultivars Embryos Explants genes Genetic transformation genetically modified organisms genotype Genotypes Germplasm Histology insecticidal proteins Kanamycin Legumes Life Sciences Morphology Original Article Pest resistance Pests Phaseolus vulgaris Plant breeding plumule Progress in genomics promoter regions Proteins selection pressure transcriptomics and breeding of crops Transgenic plants |
| title | Genetic transformation of common beans (Phaseolus vulgaris L.) through Agrobacterium tumefaciens carrying Cry1Ab gene |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T00%3A06%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20transformation%20of%20common%20beans%20(Phaseolus%20vulgaris%20L.)%20through%20Agrobacterium%20tumefaciens%20carrying%20Cry1Ab%20gene&rft.jtitle=Molecular%20biology%20reports&rft.au=Sa%C4%9Flam%20Y%C4%B1lmaz,%20Sevil&rft.date=2022-07-01&rft.volume=49&rft.issue=7&rft.spage=7195&rft.epage=7203&rft.pages=7195-7203&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-022-07637-1&rft_dat=%3Cproquest_cross%3E2718274596%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2686429122&rft_id=info:pmid/&rfr_iscdi=true |