3D printed controllable microporous scaffolds support embryonic development in vitro
Little is known about the complex molecular and cellular events occurring during implantation, which represents a critical step for pregnancy. The conventional 2D culture could not support postimplantation embryos' normal development, and 3D conditions shed light into the “black box”. 3D printi...
Gespeichert in:
| Veröffentlicht in: | Journal of cellular physiology 2022-08, Vol.237 (8), p.3408-3420 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3420 |
|---|---|
| container_issue | 8 |
| container_start_page | 3408 |
| container_title | Journal of cellular physiology |
| container_volume | 237 |
| creator | Guo, Jia Li, Yuanyuan Gao, Zili Lyu, Jiawei Liu, Wenli Duan, Yongchao Zhou, Lixun Gu, Qi |
| description | Little is known about the complex molecular and cellular events occurring during implantation, which represents a critical step for pregnancy. The conventional 2D culture could not support postimplantation embryos' normal development, and 3D conditions shed light into the “black box”. 3D printing technology has been widely used in recapitulating the structure and function of native tissues in vitro. Here, we 3D printed anisotropic microporous scaffolds to culture embryos by manipulating the advancing angle between printed layers, which affected embryo development. The 30° and 60° scaffolds promote embryo development with moderate embryo‐scaffold attachments. T‐positive cells and FOXA2‐positive cells were observed to appear in the posterior region of the embryo and migrated to the anterior region of the embryo on day 7. These findings demonstrate a 3D printed stand that supports embryonic development in vitro and the critical role of 3D architecture for embryo implantation, in which additive manufacturing is a versatile tool. |
| doi_str_mv | 10.1002/jcp.30810 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2676555160</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2701556251</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3880-d9958c24c0dc7a7b01e294e1ed362e54ffff3652bd0d22348ac73370648e6eec3</originalsourceid><addsrcrecordid>eNp1kEFP3DAQhS1UBAvl0D9QReoFDmHHduzEx2oLlGolOMDZSuyJlFUSp3ZCtf--Q5f2UIm5eDT-9PTeY-wTh2sOINY7N11LqDgcsRUHU-aFVuIDW9Efz40q-Ck7S2kHAMZIecJOpdLG6KJasSf5LZtiN87oMxfGOYa-r5ses6FzMUwhhiVlydVtG3pP2zLRbc5waOI-jJ3LPL5gH6YBxznrxuylI4mP7Lit-4QXb-85e769edp8z7cPd_ebr9vcyaqC3BujKicKB96VddkAR2EK5OilFqiKlkZSksaDF0IWVe1KKUsg46gRnTxnlwfdKYafC6bZDl1ySAlGJN9W6FIrpbgGQr_8h-7CEkdyZ0UJXCktFCfq6kBR9pQitpa6Geq4txzsa9WWqrZ_qib285vi0gzo_5F_uyVgfQB-dT3u31eyPzaPB8nfuEaIWg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2701556251</pqid></control><display><type>article</type><title>3D printed controllable microporous scaffolds support embryonic development in vitro</title><source>Access via Wiley Online Library</source><creator>Guo, Jia ; Li, Yuanyuan ; Gao, Zili ; Lyu, Jiawei ; Liu, Wenli ; Duan, Yongchao ; Zhou, Lixun ; Gu, Qi</creator><creatorcontrib>Guo, Jia ; Li, Yuanyuan ; Gao, Zili ; Lyu, Jiawei ; Liu, Wenli ; Duan, Yongchao ; Zhou, Lixun ; Gu, Qi</creatorcontrib><description>Little is known about the complex molecular and cellular events occurring during implantation, which represents a critical step for pregnancy. The conventional 2D culture could not support postimplantation embryos' normal development, and 3D conditions shed light into the “black box”. 3D printing technology has been widely used in recapitulating the structure and function of native tissues in vitro. Here, we 3D printed anisotropic microporous scaffolds to culture embryos by manipulating the advancing angle between printed layers, which affected embryo development. The 30° and 60° scaffolds promote embryo development with moderate embryo‐scaffold attachments. T‐positive cells and FOXA2‐positive cells were observed to appear in the posterior region of the embryo and migrated to the anterior region of the embryo on day 7. These findings demonstrate a 3D printed stand that supports embryonic development in vitro and the critical role of 3D architecture for embryo implantation, in which additive manufacturing is a versatile tool.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.30810</identifier><identifier>PMID: 35699648</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>3D printing ; anisotropic microporous scaffolds ; Cell culture ; embryo implantation ; Embryogenesis ; Embryonic growth stage ; Embryos ; Implantation ; in vitro culture ; Scaffolds ; Structure-function relationships ; Three dimensional printing</subject><ispartof>Journal of cellular physiology, 2022-08, Vol.237 (8), p.3408-3420</ispartof><rights>2022 The Authors. published by Wiley Periodicals LLC.</rights><rights>2022 The Authors. Journal of Cellular Physiology published by Wiley Periodicals LLC.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3880-d9958c24c0dc7a7b01e294e1ed362e54ffff3652bd0d22348ac73370648e6eec3</citedby><cites>FETCH-LOGICAL-c3880-d9958c24c0dc7a7b01e294e1ed362e54ffff3652bd0d22348ac73370648e6eec3</cites><orcidid>0000-0001-9387-9525</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.30810$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.30810$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35699648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Jia</creatorcontrib><creatorcontrib>Li, Yuanyuan</creatorcontrib><creatorcontrib>Gao, Zili</creatorcontrib><creatorcontrib>Lyu, Jiawei</creatorcontrib><creatorcontrib>Liu, Wenli</creatorcontrib><creatorcontrib>Duan, Yongchao</creatorcontrib><creatorcontrib>Zhou, Lixun</creatorcontrib><creatorcontrib>Gu, Qi</creatorcontrib><title>3D printed controllable microporous scaffolds support embryonic development in vitro</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>Little is known about the complex molecular and cellular events occurring during implantation, which represents a critical step for pregnancy. The conventional 2D culture could not support postimplantation embryos' normal development, and 3D conditions shed light into the “black box”. 3D printing technology has been widely used in recapitulating the structure and function of native tissues in vitro. Here, we 3D printed anisotropic microporous scaffolds to culture embryos by manipulating the advancing angle between printed layers, which affected embryo development. The 30° and 60° scaffolds promote embryo development with moderate embryo‐scaffold attachments. T‐positive cells and FOXA2‐positive cells were observed to appear in the posterior region of the embryo and migrated to the anterior region of the embryo on day 7. These findings demonstrate a 3D printed stand that supports embryonic development in vitro and the critical role of 3D architecture for embryo implantation, in which additive manufacturing is a versatile tool.</description><subject>3D printing</subject><subject>anisotropic microporous scaffolds</subject><subject>Cell culture</subject><subject>embryo implantation</subject><subject>Embryogenesis</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Implantation</subject><subject>in vitro culture</subject><subject>Scaffolds</subject><subject>Structure-function relationships</subject><subject>Three dimensional printing</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNp1kEFP3DAQhS1UBAvl0D9QReoFDmHHduzEx2oLlGolOMDZSuyJlFUSp3ZCtf--Q5f2UIm5eDT-9PTeY-wTh2sOINY7N11LqDgcsRUHU-aFVuIDW9Efz40q-Ck7S2kHAMZIecJOpdLG6KJasSf5LZtiN87oMxfGOYa-r5ses6FzMUwhhiVlydVtG3pP2zLRbc5waOI-jJ3LPL5gH6YBxznrxuylI4mP7Lit-4QXb-85e769edp8z7cPd_ebr9vcyaqC3BujKicKB96VddkAR2EK5OilFqiKlkZSksaDF0IWVe1KKUsg46gRnTxnlwfdKYafC6bZDl1ySAlGJN9W6FIrpbgGQr_8h-7CEkdyZ0UJXCktFCfq6kBR9pQitpa6Geq4txzsa9WWqrZ_qib285vi0gzo_5F_uyVgfQB-dT3u31eyPzaPB8nfuEaIWg</recordid><startdate>202208</startdate><enddate>202208</enddate><creator>Guo, Jia</creator><creator>Li, Yuanyuan</creator><creator>Gao, Zili</creator><creator>Lyu, Jiawei</creator><creator>Liu, Wenli</creator><creator>Duan, Yongchao</creator><creator>Zhou, Lixun</creator><creator>Gu, Qi</creator><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9387-9525</orcidid></search><sort><creationdate>202208</creationdate><title>3D printed controllable microporous scaffolds support embryonic development in vitro</title><author>Guo, Jia ; Li, Yuanyuan ; Gao, Zili ; Lyu, Jiawei ; Liu, Wenli ; Duan, Yongchao ; Zhou, Lixun ; Gu, Qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3880-d9958c24c0dc7a7b01e294e1ed362e54ffff3652bd0d22348ac73370648e6eec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>3D printing</topic><topic>anisotropic microporous scaffolds</topic><topic>Cell culture</topic><topic>embryo implantation</topic><topic>Embryogenesis</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Implantation</topic><topic>in vitro culture</topic><topic>Scaffolds</topic><topic>Structure-function relationships</topic><topic>Three dimensional printing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Jia</creatorcontrib><creatorcontrib>Li, Yuanyuan</creatorcontrib><creatorcontrib>Gao, Zili</creatorcontrib><creatorcontrib>Lyu, Jiawei</creatorcontrib><creatorcontrib>Liu, Wenli</creatorcontrib><creatorcontrib>Duan, Yongchao</creatorcontrib><creatorcontrib>Zhou, Lixun</creatorcontrib><creatorcontrib>Gu, Qi</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Jia</au><au>Li, Yuanyuan</au><au>Gao, Zili</au><au>Lyu, Jiawei</au><au>Liu, Wenli</au><au>Duan, Yongchao</au><au>Zhou, Lixun</au><au>Gu, Qi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>3D printed controllable microporous scaffolds support embryonic development in vitro</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J Cell Physiol</addtitle><date>2022-08</date><risdate>2022</risdate><volume>237</volume><issue>8</issue><spage>3408</spage><epage>3420</epage><pages>3408-3420</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Little is known about the complex molecular and cellular events occurring during implantation, which represents a critical step for pregnancy. The conventional 2D culture could not support postimplantation embryos' normal development, and 3D conditions shed light into the “black box”. 3D printing technology has been widely used in recapitulating the structure and function of native tissues in vitro. Here, we 3D printed anisotropic microporous scaffolds to culture embryos by manipulating the advancing angle between printed layers, which affected embryo development. The 30° and 60° scaffolds promote embryo development with moderate embryo‐scaffold attachments. T‐positive cells and FOXA2‐positive cells were observed to appear in the posterior region of the embryo and migrated to the anterior region of the embryo on day 7. These findings demonstrate a 3D printed stand that supports embryonic development in vitro and the critical role of 3D architecture for embryo implantation, in which additive manufacturing is a versatile tool.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35699648</pmid><doi>10.1002/jcp.30810</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-9387-9525</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9541 |
| ispartof | Journal of cellular physiology, 2022-08, Vol.237 (8), p.3408-3420 |
| issn | 0021-9541 1097-4652 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2676555160 |
| source | Access via Wiley Online Library |
| subjects | 3D printing anisotropic microporous scaffolds Cell culture embryo implantation Embryogenesis Embryonic growth stage Embryos Implantation in vitro culture Scaffolds Structure-function relationships Three dimensional printing |
| title | 3D printed controllable microporous scaffolds support embryonic development in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T11%3A28%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=3D%20printed%20controllable%20microporous%20scaffolds%20support%20embryonic%20development%20in%20vitro&rft.jtitle=Journal%20of%20cellular%20physiology&rft.au=Guo,%20Jia&rft.date=2022-08&rft.volume=237&rft.issue=8&rft.spage=3408&rft.epage=3420&rft.pages=3408-3420&rft.issn=0021-9541&rft.eissn=1097-4652&rft_id=info:doi/10.1002/jcp.30810&rft_dat=%3Cproquest_cross%3E2701556251%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2701556251&rft_id=info:pmid/35699648&rfr_iscdi=true |