TRY intron2 determined its expression in inflorescence activated by SPL9 and MADS-box genes in Arabidopsis
Plant trichomes are specialized epidermal cells that protect plants from insects and pathogens. In Arabidopsis, epidermal hairs decrease as internodes increase in height, with only few epidermal hairs produced on the sepals abaxial surface of the early flowers. TRIPTYCHON (TRY) is known to be a nega...
Gespeichert in:
Veröffentlicht in: | Plant science (Limerick) 2022-08, Vol.321, p.111311-111311, Article 111311 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 111311 |
---|---|
container_issue | |
container_start_page | 111311 |
container_title | Plant science (Limerick) |
container_volume | 321 |
creator | Cui, Wenrui Chen, Zhiwen Shangguan, Xiaoxia Li, Taotao Wang, Lingjian Xue, Xueyi Cao, Junfeng |
description | Plant trichomes are specialized epidermal cells that protect plants from insects and pathogens. In Arabidopsis, epidermal hairs decrease as internodes increase in height, with only few epidermal hairs produced on the sepals abaxial surface of the early flowers. TRIPTYCHON (TRY) is known to be a negative regulator of epidermal hair development in Arabidopsis, suppressing the formation of ectopic epidermal hairs in the inflorescence. Here, we reported that the second intron of TRY gene plays a critical role in trichome spatial distribution in Arabidopsis. The expression of TRY rises with the increasing stem nodes and reaches the peak in the inflorescence, while the trichomes distribution decrease. The transgenic plants showed that TRY promoter could only drive the genomic instead of coding sequences combined with GUS reporter gene, which indicates that the regulatory elements of TRY expression in inflorescence could be located in the intron regions. Multiple SPLs and MADS-box binding sites were found in the TRY intron2 sequence. Further genetic and biochemistry assays revealed that the flowering-related genes such as SPL9 could bind to these cis-elements directly, contributing to the TRY spatial expression. Since cotton fiber and Arabidopsis trichomes share similar regulatory mechanism, extended analysis showed that the intron2 of cotton TRY genes also contain the cis-elements. Thus, the introns harboring the transcription element may be the general way to regulate the gene expression in different plants and provides molecular clues for the related crops’ traits design.
•TRY expresses high in the inflorescence contributing to no trichomes in this part, while TRY promoter could only drive the genomic sequence instead of CDS expressing in the inflorescence.•Transgenic and biochemistry assays showed that the SPL-binding motifs and MADS box exist in the TRY inron2, which could be activated by flower related genes.•The cotton GhTRY genes introns also contain the cis-elements and the growth of trichomes through the introns may be a general way in plants. |
doi_str_mv | 10.1016/j.plantsci.2022.111311 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2676551820</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168945222001352</els_id><sourcerecordid>2676551820</sourcerecordid><originalsourceid>FETCH-LOGICAL-c434t-35a174ab594e27121b5a4e56615bb7f0aaf6118881315946abe16d7ddef303b23</originalsourceid><addsrcrecordid>eNqFkMFO4zAQhq0VaCksr4B85JLicWInuW0F7IJUBKLdAyfLjicrV20cbBfB2-OqwBVpJGuk75_xfIScAZsCA3mxmo5rPaTYuSlnnE8BoAT4QSbQ1GXBuWgPyCSDTdFWgh-R4xhXjDEuRP2THJVCtrIFmJDV8vGJuiEFP3BqMWHYuAEtdSlSfB0Dxuj8kIlc_drnvsOhQ6q75F50yqR5o4uHeUv1YOnd7GpRGP9K_-OAcZeaBW2c9WN08Rc57PU64unHe0L-_bleXt4U8_u_t5ezedFVZZWKUmioK21EWyGvgYMRukIhJQhj6p5p3UuApmnywZmR2iBIW1uLfclKw8sTcr6fOwb_vMWY1MblX6-zL_TbqLispRDQcJZRuUe74GMM2KsxuI0ObwqY2nlWK_XpWe08q73nHDz72LE1G7RfsU-xGfi9BzBf-uIwqDxiZ866gF1S1rvvdrwDzDKRjA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2676551820</pqid></control><display><type>article</type><title>TRY intron2 determined its expression in inflorescence activated by SPL9 and MADS-box genes in Arabidopsis</title><source>Access via ScienceDirect (Elsevier)</source><creator>Cui, Wenrui ; Chen, Zhiwen ; Shangguan, Xiaoxia ; Li, Taotao ; Wang, Lingjian ; Xue, Xueyi ; Cao, Junfeng</creator><creatorcontrib>Cui, Wenrui ; Chen, Zhiwen ; Shangguan, Xiaoxia ; Li, Taotao ; Wang, Lingjian ; Xue, Xueyi ; Cao, Junfeng</creatorcontrib><description>Plant trichomes are specialized epidermal cells that protect plants from insects and pathogens. In Arabidopsis, epidermal hairs decrease as internodes increase in height, with only few epidermal hairs produced on the sepals abaxial surface of the early flowers. TRIPTYCHON (TRY) is known to be a negative regulator of epidermal hair development in Arabidopsis, suppressing the formation of ectopic epidermal hairs in the inflorescence. Here, we reported that the second intron of TRY gene plays a critical role in trichome spatial distribution in Arabidopsis. The expression of TRY rises with the increasing stem nodes and reaches the peak in the inflorescence, while the trichomes distribution decrease. The transgenic plants showed that TRY promoter could only drive the genomic instead of coding sequences combined with GUS reporter gene, which indicates that the regulatory elements of TRY expression in inflorescence could be located in the intron regions. Multiple SPLs and MADS-box binding sites were found in the TRY intron2 sequence. Further genetic and biochemistry assays revealed that the flowering-related genes such as SPL9 could bind to these cis-elements directly, contributing to the TRY spatial expression. Since cotton fiber and Arabidopsis trichomes share similar regulatory mechanism, extended analysis showed that the intron2 of cotton TRY genes also contain the cis-elements. Thus, the introns harboring the transcription element may be the general way to regulate the gene expression in different plants and provides molecular clues for the related crops’ traits design.
•TRY expresses high in the inflorescence contributing to no trichomes in this part, while TRY promoter could only drive the genomic sequence instead of CDS expressing in the inflorescence.•Transgenic and biochemistry assays showed that the SPL-binding motifs and MADS box exist in the TRY inron2, which could be activated by flower related genes.•The cotton GhTRY genes introns also contain the cis-elements and the growth of trichomes through the introns may be a general way in plants.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2022.111311</identifier><identifier>PMID: 35696911</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Gene regulation ; Intron ; SQUMOSA PROMOTER BINDING PROTEIN LIKE 9 (SPL9) ; Trichome ; TRIPTYCHON(TRY)</subject><ispartof>Plant science (Limerick), 2022-08, Vol.321, p.111311-111311, Article 111311</ispartof><rights>2022 Elsevier B.V.</rights><rights>Copyright © 2022 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-35a174ab594e27121b5a4e56615bb7f0aaf6118881315946abe16d7ddef303b23</citedby><cites>FETCH-LOGICAL-c434t-35a174ab594e27121b5a4e56615bb7f0aaf6118881315946abe16d7ddef303b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.plantsci.2022.111311$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35696911$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cui, Wenrui</creatorcontrib><creatorcontrib>Chen, Zhiwen</creatorcontrib><creatorcontrib>Shangguan, Xiaoxia</creatorcontrib><creatorcontrib>Li, Taotao</creatorcontrib><creatorcontrib>Wang, Lingjian</creatorcontrib><creatorcontrib>Xue, Xueyi</creatorcontrib><creatorcontrib>Cao, Junfeng</creatorcontrib><title>TRY intron2 determined its expression in inflorescence activated by SPL9 and MADS-box genes in Arabidopsis</title><title>Plant science (Limerick)</title><addtitle>Plant Sci</addtitle><description>Plant trichomes are specialized epidermal cells that protect plants from insects and pathogens. In Arabidopsis, epidermal hairs decrease as internodes increase in height, with only few epidermal hairs produced on the sepals abaxial surface of the early flowers. TRIPTYCHON (TRY) is known to be a negative regulator of epidermal hair development in Arabidopsis, suppressing the formation of ectopic epidermal hairs in the inflorescence. Here, we reported that the second intron of TRY gene plays a critical role in trichome spatial distribution in Arabidopsis. The expression of TRY rises with the increasing stem nodes and reaches the peak in the inflorescence, while the trichomes distribution decrease. The transgenic plants showed that TRY promoter could only drive the genomic instead of coding sequences combined with GUS reporter gene, which indicates that the regulatory elements of TRY expression in inflorescence could be located in the intron regions. Multiple SPLs and MADS-box binding sites were found in the TRY intron2 sequence. Further genetic and biochemistry assays revealed that the flowering-related genes such as SPL9 could bind to these cis-elements directly, contributing to the TRY spatial expression. Since cotton fiber and Arabidopsis trichomes share similar regulatory mechanism, extended analysis showed that the intron2 of cotton TRY genes also contain the cis-elements. Thus, the introns harboring the transcription element may be the general way to regulate the gene expression in different plants and provides molecular clues for the related crops’ traits design.
•TRY expresses high in the inflorescence contributing to no trichomes in this part, while TRY promoter could only drive the genomic sequence instead of CDS expressing in the inflorescence.•Transgenic and biochemistry assays showed that the SPL-binding motifs and MADS box exist in the TRY inron2, which could be activated by flower related genes.•The cotton GhTRY genes introns also contain the cis-elements and the growth of trichomes through the introns may be a general way in plants.</description><subject>Gene regulation</subject><subject>Intron</subject><subject>SQUMOSA PROMOTER BINDING PROTEIN LIKE 9 (SPL9)</subject><subject>Trichome</subject><subject>TRIPTYCHON(TRY)</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkMFO4zAQhq0VaCksr4B85JLicWInuW0F7IJUBKLdAyfLjicrV20cbBfB2-OqwBVpJGuk75_xfIScAZsCA3mxmo5rPaTYuSlnnE8BoAT4QSbQ1GXBuWgPyCSDTdFWgh-R4xhXjDEuRP2THJVCtrIFmJDV8vGJuiEFP3BqMWHYuAEtdSlSfB0Dxuj8kIlc_drnvsOhQ6q75F50yqR5o4uHeUv1YOnd7GpRGP9K_-OAcZeaBW2c9WN08Rc57PU64unHe0L-_bleXt4U8_u_t5ezedFVZZWKUmioK21EWyGvgYMRukIhJQhj6p5p3UuApmnywZmR2iBIW1uLfclKw8sTcr6fOwb_vMWY1MblX6-zL_TbqLispRDQcJZRuUe74GMM2KsxuI0ObwqY2nlWK_XpWe08q73nHDz72LE1G7RfsU-xGfi9BzBf-uIwqDxiZ866gF1S1rvvdrwDzDKRjA</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>Cui, Wenrui</creator><creator>Chen, Zhiwen</creator><creator>Shangguan, Xiaoxia</creator><creator>Li, Taotao</creator><creator>Wang, Lingjian</creator><creator>Xue, Xueyi</creator><creator>Cao, Junfeng</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20220801</creationdate><title>TRY intron2 determined its expression in inflorescence activated by SPL9 and MADS-box genes in Arabidopsis</title><author>Cui, Wenrui ; Chen, Zhiwen ; Shangguan, Xiaoxia ; Li, Taotao ; Wang, Lingjian ; Xue, Xueyi ; Cao, Junfeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-35a174ab594e27121b5a4e56615bb7f0aaf6118881315946abe16d7ddef303b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Gene regulation</topic><topic>Intron</topic><topic>SQUMOSA PROMOTER BINDING PROTEIN LIKE 9 (SPL9)</topic><topic>Trichome</topic><topic>TRIPTYCHON(TRY)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cui, Wenrui</creatorcontrib><creatorcontrib>Chen, Zhiwen</creatorcontrib><creatorcontrib>Shangguan, Xiaoxia</creatorcontrib><creatorcontrib>Li, Taotao</creatorcontrib><creatorcontrib>Wang, Lingjian</creatorcontrib><creatorcontrib>Xue, Xueyi</creatorcontrib><creatorcontrib>Cao, Junfeng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cui, Wenrui</au><au>Chen, Zhiwen</au><au>Shangguan, Xiaoxia</au><au>Li, Taotao</au><au>Wang, Lingjian</au><au>Xue, Xueyi</au><au>Cao, Junfeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TRY intron2 determined its expression in inflorescence activated by SPL9 and MADS-box genes in Arabidopsis</atitle><jtitle>Plant science (Limerick)</jtitle><addtitle>Plant Sci</addtitle><date>2022-08-01</date><risdate>2022</risdate><volume>321</volume><spage>111311</spage><epage>111311</epage><pages>111311-111311</pages><artnum>111311</artnum><issn>0168-9452</issn><eissn>1873-2259</eissn><abstract>Plant trichomes are specialized epidermal cells that protect plants from insects and pathogens. In Arabidopsis, epidermal hairs decrease as internodes increase in height, with only few epidermal hairs produced on the sepals abaxial surface of the early flowers. TRIPTYCHON (TRY) is known to be a negative regulator of epidermal hair development in Arabidopsis, suppressing the formation of ectopic epidermal hairs in the inflorescence. Here, we reported that the second intron of TRY gene plays a critical role in trichome spatial distribution in Arabidopsis. The expression of TRY rises with the increasing stem nodes and reaches the peak in the inflorescence, while the trichomes distribution decrease. The transgenic plants showed that TRY promoter could only drive the genomic instead of coding sequences combined with GUS reporter gene, which indicates that the regulatory elements of TRY expression in inflorescence could be located in the intron regions. Multiple SPLs and MADS-box binding sites were found in the TRY intron2 sequence. Further genetic and biochemistry assays revealed that the flowering-related genes such as SPL9 could bind to these cis-elements directly, contributing to the TRY spatial expression. Since cotton fiber and Arabidopsis trichomes share similar regulatory mechanism, extended analysis showed that the intron2 of cotton TRY genes also contain the cis-elements. Thus, the introns harboring the transcription element may be the general way to regulate the gene expression in different plants and provides molecular clues for the related crops’ traits design.
•TRY expresses high in the inflorescence contributing to no trichomes in this part, while TRY promoter could only drive the genomic sequence instead of CDS expressing in the inflorescence.•Transgenic and biochemistry assays showed that the SPL-binding motifs and MADS box exist in the TRY inron2, which could be activated by flower related genes.•The cotton GhTRY genes introns also contain the cis-elements and the growth of trichomes through the introns may be a general way in plants.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>35696911</pmid><doi>10.1016/j.plantsci.2022.111311</doi><tpages>1</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0168-9452 |
ispartof | Plant science (Limerick), 2022-08, Vol.321, p.111311-111311, Article 111311 |
issn | 0168-9452 1873-2259 |
language | eng |
recordid | cdi_proquest_miscellaneous_2676551820 |
source | Access via ScienceDirect (Elsevier) |
subjects | Gene regulation Intron SQUMOSA PROMOTER BINDING PROTEIN LIKE 9 (SPL9) Trichome TRIPTYCHON(TRY) |
title | TRY intron2 determined its expression in inflorescence activated by SPL9 and MADS-box genes in Arabidopsis |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T12%3A41%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=TRY%20intron2%20determined%20its%20expression%20in%20inflorescence%20activated%20by%20SPL9%20and%20MADS-box%20genes%20in%20Arabidopsis&rft.jtitle=Plant%20science%20(Limerick)&rft.au=Cui,%20Wenrui&rft.date=2022-08-01&rft.volume=321&rft.spage=111311&rft.epage=111311&rft.pages=111311-111311&rft.artnum=111311&rft.issn=0168-9452&rft.eissn=1873-2259&rft_id=info:doi/10.1016/j.plantsci.2022.111311&rft_dat=%3Cproquest_cross%3E2676551820%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2676551820&rft_id=info:pmid/35696911&rft_els_id=S0168945222001352&rfr_iscdi=true |