Effects of ROS and caspase-3-like protein on the growth and aerenchyma formation of Potamogeton perfoliatus stem
Aerenchyma formation plays an important role in the survival of Potamogeton perfoliatus in submerged environment. To understand the regulatory role of reactive oxygen species (ROS) and caspase 3-like protein signaling molecules in aerenchyma formation, we investigated the effects of exogenous NADPH...
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| Veröffentlicht in: | Protoplasma 2023, Vol.260 (1), p.307-325 |
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| creator | Xie, Qinmi Yuan, Zhongxun Hou, Hui Zhao, Hongliang Chen, Hao Ni, Xilu |
| description | Aerenchyma formation plays an important role in the survival of
Potamogeton perfoliatus
in submerged environment. To understand the regulatory role of reactive oxygen species (ROS) and caspase 3-like protein signaling molecules in aerenchyma formation, we investigated the effects of exogenous NADPH oxidase inhibitor (diphenyleneiodonium chloride, DPI), catalase inhibitor (3-amino-1,2,4-triazole, AT), and caspase-3-like protein inhibitor (AC-DEVD-CHO, DEVD) on morphological and physiological characteristics and aerenchyma formation in
P. perfoliatus
. The results showed that after DPI treatment, caspase-3-like protein activity decreased, ROS-related enzyme activities increased, and H
2
O
2
content decreased, thereby inhibiting aerenchyma formation. When the concentration of DPI was approximately 1 μmol/L, the inhibitory effect was the most obvious. On the contrary, after the AT treatment, caspase-3-like protein activity increased, ROS-related enzyme activities decreased, and the H
2
O
2
content increased, ultimately promoting aerenchyma formation, and the promotion was the most obvious under treatment with approximately 500 μmol/L AT. After DEVD treatment, the inhibition of vegetative growth caused by DPI or AT treatment was alleviated, significantly reducing caspase-3-like activity and inhibiting aerenchyma development. The results of this study show that ROS has a positive regulatory effect on aerenchyma formation, and caspase-3-like protein is activated to promote ROS-mediated aerenchyma formation. This experiment provides a new theoretical basis for further exploration of the signal transduction effects of ROS and caspase-3-like protein in plant cells and their roles in plant development. |
| doi_str_mv | 10.1007/s00709-022-01780-z |
| format | Article |
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Potamogeton perfoliatus
in submerged environment. To understand the regulatory role of reactive oxygen species (ROS) and caspase 3-like protein signaling molecules in aerenchyma formation, we investigated the effects of exogenous NADPH oxidase inhibitor (diphenyleneiodonium chloride, DPI), catalase inhibitor (3-amino-1,2,4-triazole, AT), and caspase-3-like protein inhibitor (AC-DEVD-CHO, DEVD) on morphological and physiological characteristics and aerenchyma formation in
P. perfoliatus
. The results showed that after DPI treatment, caspase-3-like protein activity decreased, ROS-related enzyme activities increased, and H
2
O
2
content decreased, thereby inhibiting aerenchyma formation. When the concentration of DPI was approximately 1 μmol/L, the inhibitory effect was the most obvious. On the contrary, after the AT treatment, caspase-3-like protein activity increased, ROS-related enzyme activities decreased, and the H
2
O
2
content increased, ultimately promoting aerenchyma formation, and the promotion was the most obvious under treatment with approximately 500 μmol/L AT. After DEVD treatment, the inhibition of vegetative growth caused by DPI or AT treatment was alleviated, significantly reducing caspase-3-like activity and inhibiting aerenchyma development. The results of this study show that ROS has a positive regulatory effect on aerenchyma formation, and caspase-3-like protein is activated to promote ROS-mediated aerenchyma formation. This experiment provides a new theoretical basis for further exploration of the signal transduction effects of ROS and caspase-3-like protein in plant cells and their roles in plant development.</description><identifier>ISSN: 0033-183X</identifier><identifier>EISSN: 1615-6102</identifier><identifier>DOI: 10.1007/s00709-022-01780-z</identifier><identifier>PMID: 35689107</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Biomedical and Life Sciences ; Caspase 3 - metabolism ; Caspase-3 ; Catalase ; Cell Biology ; Enzymatic activity ; Enzymes ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Life Sciences ; NAD(P)H oxidase ; Original Article ; Physical characteristics ; Plant cells ; Plant Roots - metabolism ; Plant Sciences ; Potamogeton perfoliatus ; Potamogetonaceae - metabolism ; Proteins ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Signal transduction ; Zoology</subject><ispartof>Protoplasma, 2023, Vol.260 (1), p.307-325</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c305t-797936e0843a04968a90648e42bd35c6169d7b411187b09a139194acd6fa13f93</citedby><cites>FETCH-LOGICAL-c305t-797936e0843a04968a90648e42bd35c6169d7b411187b09a139194acd6fa13f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00709-022-01780-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00709-022-01780-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35689107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Qinmi</creatorcontrib><creatorcontrib>Yuan, Zhongxun</creatorcontrib><creatorcontrib>Hou, Hui</creatorcontrib><creatorcontrib>Zhao, Hongliang</creatorcontrib><creatorcontrib>Chen, Hao</creatorcontrib><creatorcontrib>Ni, Xilu</creatorcontrib><title>Effects of ROS and caspase-3-like protein on the growth and aerenchyma formation of Potamogeton perfoliatus stem</title><title>Protoplasma</title><addtitle>Protoplasma</addtitle><addtitle>Protoplasma</addtitle><description>Aerenchyma formation plays an important role in the survival of
Potamogeton perfoliatus
in submerged environment. To understand the regulatory role of reactive oxygen species (ROS) and caspase 3-like protein signaling molecules in aerenchyma formation, we investigated the effects of exogenous NADPH oxidase inhibitor (diphenyleneiodonium chloride, DPI), catalase inhibitor (3-amino-1,2,4-triazole, AT), and caspase-3-like protein inhibitor (AC-DEVD-CHO, DEVD) on morphological and physiological characteristics and aerenchyma formation in
P. perfoliatus
. The results showed that after DPI treatment, caspase-3-like protein activity decreased, ROS-related enzyme activities increased, and H
2
O
2
content decreased, thereby inhibiting aerenchyma formation. When the concentration of DPI was approximately 1 μmol/L, the inhibitory effect was the most obvious. On the contrary, after the AT treatment, caspase-3-like protein activity increased, ROS-related enzyme activities decreased, and the H
2
O
2
content increased, ultimately promoting aerenchyma formation, and the promotion was the most obvious under treatment with approximately 500 μmol/L AT. After DEVD treatment, the inhibition of vegetative growth caused by DPI or AT treatment was alleviated, significantly reducing caspase-3-like activity and inhibiting aerenchyma development. The results of this study show that ROS has a positive regulatory effect on aerenchyma formation, and caspase-3-like protein is activated to promote ROS-mediated aerenchyma formation. This experiment provides a new theoretical basis for further exploration of the signal transduction effects of ROS and caspase-3-like protein in plant cells and their roles in plant development.</description><subject>Biomedical and Life Sciences</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase-3</subject><subject>Catalase</subject><subject>Cell Biology</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Life Sciences</subject><subject>NAD(P)H oxidase</subject><subject>Original Article</subject><subject>Physical characteristics</subject><subject>Plant cells</subject><subject>Plant Roots - metabolism</subject><subject>Plant Sciences</subject><subject>Potamogeton perfoliatus</subject><subject>Potamogetonaceae - metabolism</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Signal transduction</subject><subject>Zoology</subject><issn>0033-183X</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1P3DAQhq2Kqiy0f4BDZYkLF9NxnNjxsUILVEICtVTqzfIm493QJA62owp-PYYFKnHg4g_5mXdGfgg54HDMAdS3mBfQDIqCAVc1sPsPZMElr5jkUOyQBYAQjNfizy7Zi_EGAKoCqk9kV1Sy1hzUgkxL57BJkXpHf17-onZsaWPjZCMywfruL9Ip-ITdSP1I0wbpOvh_afMEWgw4Npu7wVLnw2BTl5kcdOWTHfwaU75OGJzvO5vmSGPC4TP56Gwf8cvzvk9-ny6vT87ZxeXZj5PvF6wRUCWmtNJCItSlsFBqWVsNsqyxLFatqBrJpW7VquSc12oF2nKhuS5t00qXz06LfXK0zc3j384Ykxm62GDf2xH9HE0hVSWBSyEzevgGvfFzGPN0plASdF3mf8xUsaWa4GMM6MwUusGGO8PBPPowWx8m-zBPPsx9Lvr6HD2vBmxfS14EZEBsgZifxjWG_73fiX0Az5aVNg</recordid><startdate>2023</startdate><enddate>2023</enddate><creator>Xie, Qinmi</creator><creator>Yuan, Zhongxun</creator><creator>Hou, Hui</creator><creator>Zhao, Hongliang</creator><creator>Chen, Hao</creator><creator>Ni, Xilu</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>2023</creationdate><title>Effects of ROS and caspase-3-like protein on the growth and aerenchyma formation of Potamogeton perfoliatus stem</title><author>Xie, Qinmi ; 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Potamogeton perfoliatus
in submerged environment. To understand the regulatory role of reactive oxygen species (ROS) and caspase 3-like protein signaling molecules in aerenchyma formation, we investigated the effects of exogenous NADPH oxidase inhibitor (diphenyleneiodonium chloride, DPI), catalase inhibitor (3-amino-1,2,4-triazole, AT), and caspase-3-like protein inhibitor (AC-DEVD-CHO, DEVD) on morphological and physiological characteristics and aerenchyma formation in
P. perfoliatus
. The results showed that after DPI treatment, caspase-3-like protein activity decreased, ROS-related enzyme activities increased, and H
2
O
2
content decreased, thereby inhibiting aerenchyma formation. When the concentration of DPI was approximately 1 μmol/L, the inhibitory effect was the most obvious. On the contrary, after the AT treatment, caspase-3-like protein activity increased, ROS-related enzyme activities decreased, and the H
2
O
2
content increased, ultimately promoting aerenchyma formation, and the promotion was the most obvious under treatment with approximately 500 μmol/L AT. After DEVD treatment, the inhibition of vegetative growth caused by DPI or AT treatment was alleviated, significantly reducing caspase-3-like activity and inhibiting aerenchyma development. The results of this study show that ROS has a positive regulatory effect on aerenchyma formation, and caspase-3-like protein is activated to promote ROS-mediated aerenchyma formation. This experiment provides a new theoretical basis for further exploration of the signal transduction effects of ROS and caspase-3-like protein in plant cells and their roles in plant development.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>35689107</pmid><doi>10.1007/s00709-022-01780-z</doi><tpages>19</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Caspase 3 - metabolism Caspase-3 Catalase Cell Biology Enzymatic activity Enzymes Hydrogen peroxide Hydrogen Peroxide - metabolism Life Sciences NAD(P)H oxidase Original Article Physical characteristics Plant cells Plant Roots - metabolism Plant Sciences Potamogeton perfoliatus Potamogetonaceae - metabolism Proteins Reactive oxygen species Reactive Oxygen Species - metabolism Signal transduction Zoology |
| title | Effects of ROS and caspase-3-like protein on the growth and aerenchyma formation of Potamogeton perfoliatus stem |
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