Aniline exposure activates receptor-interacting serine/threonineprotein kinase 1 and causes necroptosis of AML12 cells
With the increased use of aniline, potential impacts on human health cannot be ignored. The hepatotoxicity of aniline is largely unknown and the underlying mechanism remains unclear. Therefore, the aim of the present study was to investigate the hepatotoxicity of aniline and elucidate the underlying...
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| Veröffentlicht in: | Toxicology and industrial health 2022-08, Vol.38 (8), p.444-454 |
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| creator | Jin, Shuo Zhang, Tong Fu, Xinyu Duan, Zhongliang Sun, Jianwen Wang, Yue |
| description | With the increased use of aniline, potential impacts on human health cannot be ignored. The hepatotoxicity of aniline is largely unknown and the underlying mechanism remains unclear. Therefore, the aim of the present study was to investigate the hepatotoxicity of aniline and elucidate the underlying mechanism. AML12 cells were exposed to different concentrations of aniline (0, 5, 10, or 20 mM) to observe changes to reactive oxygen species (ROS) production and the expression patterns of necroptosis-related proteins (RIPK1, RIPK3, and MLKL). The potential mechanism underlying aniline-induced hepatotoxicity was explored by knockout of RIPK1. The results showed that aniline induced cytotoxicity in AML12 cells in a dose-dependent manner in addition to the production of ROS and subsequent necroptosis of AML12 cells. Silencing of RIPK1 reversed upregulation of necroptosis-related proteins in AML12 cells exposed to aniline, demonstrating that aniline-induced ROS production was related to necroptosis of AML12. Moreover, aniline promoted intracellular RIPK1 activation, suggesting that the RIPK1/ROS pathway plays an important role in aniline-induced hepatotoxicity. NAC could quench ROS and inhibit necroptosis. These results provide a scientific basis for future studies of aniline-induced hepatotoxicity for the prevention and treatment of aniline-induced cytotoxicity. |
| doi_str_mv | 10.1177/07482337221106751 |
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The hepatotoxicity of aniline is largely unknown and the underlying mechanism remains unclear. Therefore, the aim of the present study was to investigate the hepatotoxicity of aniline and elucidate the underlying mechanism. AML12 cells were exposed to different concentrations of aniline (0, 5, 10, or 20 mM) to observe changes to reactive oxygen species (ROS) production and the expression patterns of necroptosis-related proteins (RIPK1, RIPK3, and MLKL). The potential mechanism underlying aniline-induced hepatotoxicity was explored by knockout of RIPK1. The results showed that aniline induced cytotoxicity in AML12 cells in a dose-dependent manner in addition to the production of ROS and subsequent necroptosis of AML12 cells. Silencing of RIPK1 reversed upregulation of necroptosis-related proteins in AML12 cells exposed to aniline, demonstrating that aniline-induced ROS production was related to necroptosis of AML12. Moreover, aniline promoted intracellular RIPK1 activation, suggesting that the RIPK1/ROS pathway plays an important role in aniline-induced hepatotoxicity. NAC could quench ROS and inhibit necroptosis. These results provide a scientific basis for future studies of aniline-induced hepatotoxicity for the prevention and treatment of aniline-induced cytotoxicity.</description><identifier>ISSN: 0748-2337</identifier><identifier>EISSN: 1477-0393</identifier><identifier>DOI: 10.1177/07482337221106751</identifier><identifier>PMID: 35658749</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Aniline ; Cytotoxicity ; Exposure ; Hepatotoxicity ; Kinases ; Necroptosis ; Proteins ; Reactive oxygen species ; Toxicity</subject><ispartof>Toxicology and industrial health, 2022-08, Vol.38 (8), p.444-454</ispartof><rights>The Author(s) 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-3f55b589d79f14ef06145811723980965629366808f12981c5bc94880deef82f3</citedby><cites>FETCH-LOGICAL-c368t-3f55b589d79f14ef06145811723980965629366808f12981c5bc94880deef82f3</cites><orcidid>0000-0001-6080-819X ; 0000-0002-4514-4652</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/07482337221106751$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/07482337221106751$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35658749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jin, Shuo</creatorcontrib><creatorcontrib>Zhang, Tong</creatorcontrib><creatorcontrib>Fu, Xinyu</creatorcontrib><creatorcontrib>Duan, Zhongliang</creatorcontrib><creatorcontrib>Sun, Jianwen</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><title>Aniline exposure activates receptor-interacting serine/threonineprotein kinase 1 and causes necroptosis of AML12 cells</title><title>Toxicology and industrial health</title><addtitle>Toxicol Ind Health</addtitle><description>With the increased use of aniline, potential impacts on human health cannot be ignored. The hepatotoxicity of aniline is largely unknown and the underlying mechanism remains unclear. Therefore, the aim of the present study was to investigate the hepatotoxicity of aniline and elucidate the underlying mechanism. AML12 cells were exposed to different concentrations of aniline (0, 5, 10, or 20 mM) to observe changes to reactive oxygen species (ROS) production and the expression patterns of necroptosis-related proteins (RIPK1, RIPK3, and MLKL). The potential mechanism underlying aniline-induced hepatotoxicity was explored by knockout of RIPK1. The results showed that aniline induced cytotoxicity in AML12 cells in a dose-dependent manner in addition to the production of ROS and subsequent necroptosis of AML12 cells. Silencing of RIPK1 reversed upregulation of necroptosis-related proteins in AML12 cells exposed to aniline, demonstrating that aniline-induced ROS production was related to necroptosis of AML12. Moreover, aniline promoted intracellular RIPK1 activation, suggesting that the RIPK1/ROS pathway plays an important role in aniline-induced hepatotoxicity. NAC could quench ROS and inhibit necroptosis. These results provide a scientific basis for future studies of aniline-induced hepatotoxicity for the prevention and treatment of aniline-induced cytotoxicity.</description><subject>Aniline</subject><subject>Cytotoxicity</subject><subject>Exposure</subject><subject>Hepatotoxicity</subject><subject>Kinases</subject><subject>Necroptosis</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Toxicity</subject><issn>0748-2337</issn><issn>1477-0393</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kU1PGzEQhq0KVNK0P6CXyhIXLpv4Y_11jKKWVgriAueVsxmnpok3eHZR-fd4FVokECeP7GdevzMvIV85m3FuzJyZ2gopjRCcM20U_0AmvDamYtLJEzIZ36sROCOfEO8YY1or8ZGcSaWVNbWbkIdFiruYgMLfQ4dDBurbPj74HpBmaOHQd7mKqYc83qctRcgFn_e_M3SpVIfc9RAT_ROTR6Cc-rShrR-wCCRoc1cUMCLtAl1crbigLex2-JmcBr9D-PJ8Tsntj-83y5_V6vry13KxqlqpbV_JoNRaWbcxLvAaAtO8VrbMLqSzzGmlhZNaW2YDF87yVq1bV1vLNgDBiiCn5OKoW2zeD4B9s484OvAJugEboY1UTjsmC3r-Cr3rhpyKu0YYxpSQtmx1SviRKpMhZgjNIce9z48NZ80YSvMmlNLz7Vl5WO9h87_jXwoFmB0B9Ft4-fZ9xSefx5N0</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>Jin, Shuo</creator><creator>Zhang, Tong</creator><creator>Fu, Xinyu</creator><creator>Duan, Zhongliang</creator><creator>Sun, Jianwen</creator><creator>Wang, Yue</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T2</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6080-819X</orcidid><orcidid>https://orcid.org/0000-0002-4514-4652</orcidid></search><sort><creationdate>20220801</creationdate><title>Aniline exposure activates receptor-interacting serine/threonineprotein kinase 1 and causes necroptosis of AML12 cells</title><author>Jin, Shuo ; 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The hepatotoxicity of aniline is largely unknown and the underlying mechanism remains unclear. Therefore, the aim of the present study was to investigate the hepatotoxicity of aniline and elucidate the underlying mechanism. AML12 cells were exposed to different concentrations of aniline (0, 5, 10, or 20 mM) to observe changes to reactive oxygen species (ROS) production and the expression patterns of necroptosis-related proteins (RIPK1, RIPK3, and MLKL). The potential mechanism underlying aniline-induced hepatotoxicity was explored by knockout of RIPK1. The results showed that aniline induced cytotoxicity in AML12 cells in a dose-dependent manner in addition to the production of ROS and subsequent necroptosis of AML12 cells. Silencing of RIPK1 reversed upregulation of necroptosis-related proteins in AML12 cells exposed to aniline, demonstrating that aniline-induced ROS production was related to necroptosis of AML12. 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| subjects | Aniline Cytotoxicity Exposure Hepatotoxicity Kinases Necroptosis Proteins Reactive oxygen species Toxicity |
| title | Aniline exposure activates receptor-interacting serine/threonineprotein kinase 1 and causes necroptosis of AML12 cells |
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