Nanoconfined Electrochemical Collision and Catalysis of Single Enzyme inside Carbon Nanopipettes

Revealing the electrocatalytic features of single redox enzyme is significant to both fundamental biological processes and practical catalysis and sensing applications. Herein, we directly reveal the electrocatalytic current from a single enzyme inside the carbon nanopipettes via electrochemical col...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytical chemistry (Washington) 2022-06, Vol.94 (23), p.8110-8114
Hauptverfasser: Shen, Xiaoyue, Liu, Rujia, Wang, Dengchao
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 8114
container_issue 23
container_start_page 8110
container_title Analytical chemistry (Washington)
container_volume 94
creator Shen, Xiaoyue
Liu, Rujia
Wang, Dengchao
description Revealing the electrocatalytic features of single redox enzyme is significant to both fundamental biological processes and practical catalysis and sensing applications. Herein, we directly reveal the electrocatalytic current from a single enzyme inside the carbon nanopipettes via electrochemical collision strategies, based on the increased activity at nanoscale confinement. Besides the staircase current steps from surface blockage, discrete H2O2 oxidation and reduction current transients catalyzed by a single enzyme are also displayed and analyzed. The carbon nanopipette would increase the catalytic activities of enzymes and lead to a detectable current response, thus opening a new way to investigate the fundamental enzymatic mechanisms at the single enzyme level.
doi_str_mv 10.1021/acs.analchem.2c01554
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2672706362</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2677662722</sourcerecordid><originalsourceid>FETCH-LOGICAL-a291t-88ec22e936d066b45abb9a99323729ac7ff82cc6241bab88c2ba3f59992807f33</originalsourceid><addsrcrecordid>eNp9kTFv2zAQhYkgReIm-QdFIKBLFrnHo0SRY2E4aQGjGdrOyomiUgYU6Yjy4Pz6SrWToUOmW773HfAeY584LDkg_0ImLSmQN39sv0QDvCyLE7bgJUIulcJTtgAAkWMFcM4-pvQEwDlwecbORSkLpQpYsIcfFKKJoXPBttnaWzMOcVY6Qz5bRe9dcjFkFNpsRSP5fXIpi13204VHb7N1eNn3NnMhudZOxNBM8Ozcuq0dR5su2YeOfLJXx3vBft-uf62-5Zv7u--rr5ucUPMxV8oaRKuFbEHKpiipaTRpLVBUqMlUXafQGIkFb6hRymBDoiu11qig6oS4YDcH73aIzzubxrp3yVjvKdi4SzXKampCCokT-vk_9CnuhqnKf1QlJVY4U8WBMkNMabBdvR1cT8O-5lDPC9TTAvXrAvVxgSl2fZTvmt62b6HXyicADsAcf3v8rvMvrJKVVQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2677662722</pqid></control><display><type>article</type><title>Nanoconfined Electrochemical Collision and Catalysis of Single Enzyme inside Carbon Nanopipettes</title><source>ACS Publications</source><creator>Shen, Xiaoyue ; Liu, Rujia ; Wang, Dengchao</creator><creatorcontrib>Shen, Xiaoyue ; Liu, Rujia ; Wang, Dengchao</creatorcontrib><description>Revealing the electrocatalytic features of single redox enzyme is significant to both fundamental biological processes and practical catalysis and sensing applications. Herein, we directly reveal the electrocatalytic current from a single enzyme inside the carbon nanopipettes via electrochemical collision strategies, based on the increased activity at nanoscale confinement. Besides the staircase current steps from surface blockage, discrete H2O2 oxidation and reduction current transients catalyzed by a single enzyme are also displayed and analyzed. The carbon nanopipette would increase the catalytic activities of enzymes and lead to a detectable current response, thus opening a new way to investigate the fundamental enzymatic mechanisms at the single enzyme level.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.2c01554</identifier><identifier>PMID: 35648840</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Biological activity ; Carbon ; Catalysis ; Chemistry ; Electrochemistry ; Hydrogen peroxide ; Oxidation</subject><ispartof>Analytical chemistry (Washington), 2022-06, Vol.94 (23), p.8110-8114</ispartof><rights>2022 American Chemical Society</rights><rights>Copyright American Chemical Society Jun 14, 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a291t-88ec22e936d066b45abb9a99323729ac7ff82cc6241bab88c2ba3f59992807f33</citedby><cites>FETCH-LOGICAL-a291t-88ec22e936d066b45abb9a99323729ac7ff82cc6241bab88c2ba3f59992807f33</cites><orcidid>0000-0002-4909-7830</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.2c01554$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.2c01554$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,782,786,2767,27083,27931,27932,56745,56795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35648840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Xiaoyue</creatorcontrib><creatorcontrib>Liu, Rujia</creatorcontrib><creatorcontrib>Wang, Dengchao</creatorcontrib><title>Nanoconfined Electrochemical Collision and Catalysis of Single Enzyme inside Carbon Nanopipettes</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Revealing the electrocatalytic features of single redox enzyme is significant to both fundamental biological processes and practical catalysis and sensing applications. Herein, we directly reveal the electrocatalytic current from a single enzyme inside the carbon nanopipettes via electrochemical collision strategies, based on the increased activity at nanoscale confinement. Besides the staircase current steps from surface blockage, discrete H2O2 oxidation and reduction current transients catalyzed by a single enzyme are also displayed and analyzed. The carbon nanopipette would increase the catalytic activities of enzymes and lead to a detectable current response, thus opening a new way to investigate the fundamental enzymatic mechanisms at the single enzyme level.</description><subject>Biological activity</subject><subject>Carbon</subject><subject>Catalysis</subject><subject>Chemistry</subject><subject>Electrochemistry</subject><subject>Hydrogen peroxide</subject><subject>Oxidation</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kTFv2zAQhYkgReIm-QdFIKBLFrnHo0SRY2E4aQGjGdrOyomiUgYU6Yjy4Pz6SrWToUOmW773HfAeY584LDkg_0ImLSmQN39sv0QDvCyLE7bgJUIulcJTtgAAkWMFcM4-pvQEwDlwecbORSkLpQpYsIcfFKKJoXPBttnaWzMOcVY6Qz5bRe9dcjFkFNpsRSP5fXIpi13204VHb7N1eNn3NnMhudZOxNBM8Ozcuq0dR5su2YeOfLJXx3vBft-uf62-5Zv7u--rr5ucUPMxV8oaRKuFbEHKpiipaTRpLVBUqMlUXafQGIkFb6hRymBDoiu11qig6oS4YDcH73aIzzubxrp3yVjvKdi4SzXKampCCokT-vk_9CnuhqnKf1QlJVY4U8WBMkNMabBdvR1cT8O-5lDPC9TTAvXrAvVxgSl2fZTvmt62b6HXyicADsAcf3v8rvMvrJKVVQ</recordid><startdate>20220614</startdate><enddate>20220614</enddate><creator>Shen, Xiaoyue</creator><creator>Liu, Rujia</creator><creator>Wang, Dengchao</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4909-7830</orcidid></search><sort><creationdate>20220614</creationdate><title>Nanoconfined Electrochemical Collision and Catalysis of Single Enzyme inside Carbon Nanopipettes</title><author>Shen, Xiaoyue ; Liu, Rujia ; Wang, Dengchao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a291t-88ec22e936d066b45abb9a99323729ac7ff82cc6241bab88c2ba3f59992807f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biological activity</topic><topic>Carbon</topic><topic>Catalysis</topic><topic>Chemistry</topic><topic>Electrochemistry</topic><topic>Hydrogen peroxide</topic><topic>Oxidation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, Xiaoyue</creatorcontrib><creatorcontrib>Liu, Rujia</creatorcontrib><creatorcontrib>Wang, Dengchao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics &amp; Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology &amp; Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts – Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, Xiaoyue</au><au>Liu, Rujia</au><au>Wang, Dengchao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanoconfined Electrochemical Collision and Catalysis of Single Enzyme inside Carbon Nanopipettes</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2022-06-14</date><risdate>2022</risdate><volume>94</volume><issue>23</issue><spage>8110</spage><epage>8114</epage><pages>8110-8114</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Revealing the electrocatalytic features of single redox enzyme is significant to both fundamental biological processes and practical catalysis and sensing applications. Herein, we directly reveal the electrocatalytic current from a single enzyme inside the carbon nanopipettes via electrochemical collision strategies, based on the increased activity at nanoscale confinement. Besides the staircase current steps from surface blockage, discrete H2O2 oxidation and reduction current transients catalyzed by a single enzyme are also displayed and analyzed. The carbon nanopipette would increase the catalytic activities of enzymes and lead to a detectable current response, thus opening a new way to investigate the fundamental enzymatic mechanisms at the single enzyme level.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>35648840</pmid><doi>10.1021/acs.analchem.2c01554</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-4909-7830</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0003-2700
ispartof Analytical chemistry (Washington), 2022-06, Vol.94 (23), p.8110-8114
issn 0003-2700
1520-6882
language eng
recordid cdi_proquest_miscellaneous_2672706362
source ACS Publications
subjects Biological activity
Carbon
Catalysis
Chemistry
Electrochemistry
Hydrogen peroxide
Oxidation
title Nanoconfined Electrochemical Collision and Catalysis of Single Enzyme inside Carbon Nanopipettes
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T09%3A24%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nanoconfined%20Electrochemical%20Collision%20and%20Catalysis%20of%20Single%20Enzyme%20inside%20Carbon%20Nanopipettes&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Shen,%20Xiaoyue&rft.date=2022-06-14&rft.volume=94&rft.issue=23&rft.spage=8110&rft.epage=8114&rft.pages=8110-8114&rft.issn=0003-2700&rft.eissn=1520-6882&rft_id=info:doi/10.1021/acs.analchem.2c01554&rft_dat=%3Cproquest_cross%3E2677662722%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2677662722&rft_id=info:pmid/35648840&rfr_iscdi=true