Nanoconfined Electrochemical Collision and Catalysis of Single Enzyme inside Carbon Nanopipettes
Revealing the electrocatalytic features of single redox enzyme is significant to both fundamental biological processes and practical catalysis and sensing applications. Herein, we directly reveal the electrocatalytic current from a single enzyme inside the carbon nanopipettes via electrochemical col...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2022-06, Vol.94 (23), p.8110-8114 |
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| creator | Shen, Xiaoyue Liu, Rujia Wang, Dengchao |
| description | Revealing the electrocatalytic features of single redox enzyme is significant to both fundamental biological processes and practical catalysis and sensing applications. Herein, we directly reveal the electrocatalytic current from a single enzyme inside the carbon nanopipettes via electrochemical collision strategies, based on the increased activity at nanoscale confinement. Besides the staircase current steps from surface blockage, discrete H2O2 oxidation and reduction current transients catalyzed by a single enzyme are also displayed and analyzed. The carbon nanopipette would increase the catalytic activities of enzymes and lead to a detectable current response, thus opening a new way to investigate the fundamental enzymatic mechanisms at the single enzyme level. |
| doi_str_mv | 10.1021/acs.analchem.2c01554 |
| format | Article |
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The carbon nanopipette would increase the catalytic activities of enzymes and lead to a detectable current response, thus opening a new way to investigate the fundamental enzymatic mechanisms at the single enzyme level.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.2c01554</identifier><identifier>PMID: 35648840</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Biological activity ; Carbon ; Catalysis ; Chemistry ; Electrochemistry ; Hydrogen peroxide ; Oxidation</subject><ispartof>Analytical chemistry (Washington), 2022-06, Vol.94 (23), p.8110-8114</ispartof><rights>2022 American Chemical Society</rights><rights>Copyright American Chemical Society Jun 14, 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a291t-88ec22e936d066b45abb9a99323729ac7ff82cc6241bab88c2ba3f59992807f33</citedby><cites>FETCH-LOGICAL-a291t-88ec22e936d066b45abb9a99323729ac7ff82cc6241bab88c2ba3f59992807f33</cites><orcidid>0000-0002-4909-7830</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.2c01554$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.2c01554$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35648840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Xiaoyue</creatorcontrib><creatorcontrib>Liu, Rujia</creatorcontrib><creatorcontrib>Wang, Dengchao</creatorcontrib><title>Nanoconfined Electrochemical Collision and Catalysis of Single Enzyme inside Carbon Nanopipettes</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. 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| source | ACS Publications |
| subjects | Biological activity Carbon Catalysis Chemistry Electrochemistry Hydrogen peroxide Oxidation |
| title | Nanoconfined Electrochemical Collision and Catalysis of Single Enzyme inside Carbon Nanopipettes |
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