Sequence analyses of mitochondrial gene may support the existence of cryptic species within Ascaridia galli
Ascaridia galli (Nematoda: Ascaridiidae) is the most common intestinal roundworm of chickens and other birds with a worldwide distribution. Although A. galli has been extensively studied, knowledge of the genetic variation of this parasite in detail is still insufficient. The present study examined...
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| Veröffentlicht in: | Journal of helminthology 2022-06, Vol.96, p.e39-e39, Article e39 |
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| description | Ascaridia galli (Nematoda: Ascaridiidae) is the most common intestinal roundworm of chickens and other birds with a worldwide distribution. Although A. galli has been extensively studied, knowledge of the genetic variation of this parasite in detail is still insufficient. The present study examined genetic variation in the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene among A. galli isolates (n = 26) from domestic chickens in Hunan Province, China. A portion of the cox1 (pcox1) gene was amplified by polymerase chain reaction separately from adult A. galli individuals and the amplicons were subjected to sequencing from both directions. The length of the sequences of pcox1 is 441 bp. Although the intra-specific sequence variation within A. galli is 0–7.7%, the inter-specific sequence differences among other members of the infraorder Ascaridomorpha were 11.4–18.9%. Phylogenetic analyses based on the maximum likelihood method using the sequences of pcox1 confirmed that all of the Ascaridia isolates were A. galli, and also resolved three distinct clades. Taken together, the findings suggest that A. galli may represent a complex of cryptic species. Our results provide an additional genetic marker for the management of A. galli in chickens and other birds. |
| doi_str_mv | 10.1017/S0022149X2200030X |
| format | Article |
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Although A. galli has been extensively studied, knowledge of the genetic variation of this parasite in detail is still insufficient. The present study examined genetic variation in the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene among A. galli isolates (n = 26) from domestic chickens in Hunan Province, China. A portion of the cox1 (pcox1) gene was amplified by polymerase chain reaction separately from adult A. galli individuals and the amplicons were subjected to sequencing from both directions. The length of the sequences of pcox1 is 441 bp. Although the intra-specific sequence variation within A. galli is 0–7.7%, the inter-specific sequence differences among other members of the infraorder Ascaridomorpha were 11.4–18.9%. Phylogenetic analyses based on the maximum likelihood method using the sequences of pcox1 confirmed that all of the Ascaridia isolates were A. galli, and also resolved three distinct clades. Taken together, the findings suggest that A. galli may represent a complex of cryptic species. Our results provide an additional genetic marker for the management of A. galli in chickens and other birds.</description><identifier>ISSN: 0022-149X</identifier><identifier>EISSN: 1475-2697</identifier><identifier>DOI: 10.1017/S0022149X2200030X</identifier><identifier>PMID: 35641879</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Ascaridia - genetics ; Ascaridia galli ; Birds ; Chickens ; Cryptic species ; Cytochrome ; Cytochrome-c oxidase ; Cytochromes ; Genes, Mitochondrial ; Genetic diversity ; Genetic markers ; Genetic Variation ; Maximum likelihood method ; Mitochondria ; Nematodes ; Nucleotide sequence ; Parasites ; PCR ; Phylogeny ; Polymerase chain reaction ; Sequence Analysis, DNA ; Sequencing ; Short Communication ; Worms</subject><ispartof>Journal of helminthology, 2022-06, Vol.96, p.e39-e39, Article e39</ispartof><rights>Copyright © The Author(s), 2022. Published by Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c288t-41aa3e04433c02b422241858e6dee6554b52c03756653c21b4d15a713d218a943</citedby><cites>FETCH-LOGICAL-c288t-41aa3e04433c02b422241858e6dee6554b52c03756653c21b4d15a713d218a943</cites><orcidid>0000-0002-4188-6990</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0022149X2200030X/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35641879$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Y.</creatorcontrib><creatorcontrib>Lu, S.-F.</creatorcontrib><creatorcontrib>Li, J.</creatorcontrib><title>Sequence analyses of mitochondrial gene may support the existence of cryptic species within Ascaridia galli</title><title>Journal of helminthology</title><addtitle>J. Helminthol</addtitle><description>Ascaridia galli (Nematoda: Ascaridiidae) is the most common intestinal roundworm of chickens and other birds with a worldwide distribution. Although A. galli has been extensively studied, knowledge of the genetic variation of this parasite in detail is still insufficient. The present study examined genetic variation in the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene among A. galli isolates (n = 26) from domestic chickens in Hunan Province, China. A portion of the cox1 (pcox1) gene was amplified by polymerase chain reaction separately from adult A. galli individuals and the amplicons were subjected to sequencing from both directions. The length of the sequences of pcox1 is 441 bp. Although the intra-specific sequence variation within A. galli is 0–7.7%, the inter-specific sequence differences among other members of the infraorder Ascaridomorpha were 11.4–18.9%. Phylogenetic analyses based on the maximum likelihood method using the sequences of pcox1 confirmed that all of the Ascaridia isolates were A. galli, and also resolved three distinct clades. Taken together, the findings suggest that A. galli may represent a complex of cryptic species. Our results provide an additional genetic marker for the management of A. galli in chickens and other birds.</description><subject>Animals</subject><subject>Ascaridia - genetics</subject><subject>Ascaridia galli</subject><subject>Birds</subject><subject>Chickens</subject><subject>Cryptic species</subject><subject>Cytochrome</subject><subject>Cytochrome-c oxidase</subject><subject>Cytochromes</subject><subject>Genes, Mitochondrial</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Genetic Variation</subject><subject>Maximum likelihood method</subject><subject>Mitochondria</subject><subject>Nematodes</subject><subject>Nucleotide sequence</subject><subject>Parasites</subject><subject>PCR</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Sequence Analysis, DNA</subject><subject>Sequencing</subject><subject>Short Communication</subject><subject>Worms</subject><issn>0022-149X</issn><issn>1475-2697</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kctKAzEUhoMotlYfwI0E3LgZzcllZros4g0KLlTobshkTtvUuZnMoH17U60Kiqsszvd_Sf5DyDGwc2CQXDwwxjnI8Yxzxphgsx0yBJmoiMfjZJcMN-NoMx-QA-9XGwa42icDoWIJaTIekucHfOmxNkh1rcu1R0-bOa1s15hlUxfO6pIusEZa6TX1fds2rqPdEim-Wd99BANv3LrtrKG-RWOD4tV2S1vTiTfa2cJqutBlaQ_J3lyXHo-254g8XV89Xt5G0_ubu8vJNDI8TbtIgtYCmZRCGMZzyTkPj1UpxgVirJTMFTdMJCqOlTAcclmA0gmIgkOqx1KMyNmnt3VN-Jzvssp6g2Wpa2x6n_E44SI0JtKAnv5CV03vQhMfFKTAJCSBgk_KuMZ7h_OsdbbSbp0ByzabyP5sImROtuY-r7D4TnxVHwCxleoqDy0t8Ofu_7Xv1rOSaQ</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>Zhao, Y.</creator><creator>Lu, S.-F.</creator><creator>Li, J.</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7SN</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H95</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4188-6990</orcidid></search><sort><creationdate>20220601</creationdate><title>Sequence analyses of mitochondrial gene may support the existence of cryptic species within Ascaridia galli</title><author>Zhao, Y. ; Lu, S.-F. ; Li, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-41aa3e04433c02b422241858e6dee6554b52c03756653c21b4d15a713d218a943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Ascaridia - genetics</topic><topic>Ascaridia galli</topic><topic>Birds</topic><topic>Chickens</topic><topic>Cryptic species</topic><topic>Cytochrome</topic><topic>Cytochrome-c oxidase</topic><topic>Cytochromes</topic><topic>Genes, Mitochondrial</topic><topic>Genetic diversity</topic><topic>Genetic markers</topic><topic>Genetic Variation</topic><topic>Maximum likelihood method</topic><topic>Mitochondria</topic><topic>Nematodes</topic><topic>Nucleotide sequence</topic><topic>Parasites</topic><topic>PCR</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Sequence Analysis, DNA</topic><topic>Sequencing</topic><topic>Short Communication</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Y.</creatorcontrib><creatorcontrib>Lu, S.-F.</creatorcontrib><creatorcontrib>Li, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of helminthology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Y.</au><au>Lu, S.-F.</au><au>Li, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence analyses of mitochondrial gene may support the existence of cryptic species within Ascaridia galli</atitle><jtitle>Journal of helminthology</jtitle><addtitle>J. Helminthol</addtitle><date>2022-06-01</date><risdate>2022</risdate><volume>96</volume><spage>e39</spage><epage>e39</epage><pages>e39-e39</pages><artnum>e39</artnum><issn>0022-149X</issn><eissn>1475-2697</eissn><abstract>Ascaridia galli (Nematoda: Ascaridiidae) is the most common intestinal roundworm of chickens and other birds with a worldwide distribution. Although A. galli has been extensively studied, knowledge of the genetic variation of this parasite in detail is still insufficient. The present study examined genetic variation in the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene among A. galli isolates (n = 26) from domestic chickens in Hunan Province, China. A portion of the cox1 (pcox1) gene was amplified by polymerase chain reaction separately from adult A. galli individuals and the amplicons were subjected to sequencing from both directions. The length of the sequences of pcox1 is 441 bp. Although the intra-specific sequence variation within A. galli is 0–7.7%, the inter-specific sequence differences among other members of the infraorder Ascaridomorpha were 11.4–18.9%. Phylogenetic analyses based on the maximum likelihood method using the sequences of pcox1 confirmed that all of the Ascaridia isolates were A. galli, and also resolved three distinct clades. Taken together, the findings suggest that A. galli may represent a complex of cryptic species. Our results provide an additional genetic marker for the management of A. galli in chickens and other birds.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>35641879</pmid><doi>10.1017/S0022149X2200030X</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-4188-6990</orcidid></addata></record> |
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| ispartof | Journal of helminthology, 2022-06, Vol.96, p.e39-e39, Article e39 |
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| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Animals Ascaridia - genetics Ascaridia galli Birds Chickens Cryptic species Cytochrome Cytochrome-c oxidase Cytochromes Genes, Mitochondrial Genetic diversity Genetic markers Genetic Variation Maximum likelihood method Mitochondria Nematodes Nucleotide sequence Parasites PCR Phylogeny Polymerase chain reaction Sequence Analysis, DNA Sequencing Short Communication Worms |
| title | Sequence analyses of mitochondrial gene may support the existence of cryptic species within Ascaridia galli |
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