Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells
In this work Sf‐9 cells previously adapted to SF900II and EXCELL 401 serum‐free media (SFM) were grown and infected at different agitation rates in order to study the effects of power input upon cell growth, infection and production of Pr55gag particles in both SFM. Maximum cell concentration increa...
Gespeichert in:
| Veröffentlicht in: | Journal of chemical technology and biotechnology (1986) 1998-06, Vol.72 (2), p.149-158 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 158 |
|---|---|
| container_issue | 2 |
| container_start_page | 149 |
| container_title | Journal of chemical technology and biotechnology (1986) |
| container_volume | 72 |
| creator | Cruz, Pedro E. Peixoto, Cristina C. Moreira, José L. Carrondo, Manuel J. T. |
| description | In this work Sf‐9 cells previously adapted to SF900II and EXCELL 401 serum‐free media (SFM) were grown and infected at different agitation rates in order to study the effects of power input upon cell growth, infection and production of Pr55gag particles in both SFM. Maximum cell concentration increased from 3×106 to 6×106 cells cm−3 and 6·5×106 cells cm−3 when the agitation increased from 80 to 250 rpm or sparged aeration (0·01 vvm at 170 rpm) is used, clearly indicating that cell growth is limited by gas transfer. The specific productivity increased 3·5‐fold when the agitation rate was increased from 80 to 170 rpm, indicating that in SFM cell infection is also limited by gas transfer. The highest product concentration was obtained in SF900II at 120 h post‐infection (hpi). The product quality analysis showed that SF900II is the best medium for production of Pr55gag particles and that a careful optimisation of the harvest time is required. The maximum product titre was obtained at 120 hpi, 48 h after the achievement of the highest quality. © 1998 SCI |
| doi_str_mv | 10.1002/(SICI)1097-4660(199806)72:2<149::AID-JCTB886>3.0.CO;2-C |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_26711519</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>26711519</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4146-9900c2e8f42270b1d025a31bea321d2d865ad6556297c196980b4c63ee1c0c573</originalsourceid><addsrcrecordid>eNqFkN1v0zAUxSMEEmXwP-QBoe0hnT_ir4KGRgZbUUUnMVSJlyvHcSZDlnR2AvS_x1lKX0DiyVfX555z9EuStxjNMULk9PjzslieYKRElnOOjrFSEvETQRbkDc7VYnG-vMg-FjfvpORndI7mxfo1yYpHyexw8ziZIcJlRphgT5NnIXxDCHFJ-Cwpr31XDaZ3XZvqtkrvB924fhdn3eyCC2lXp9eesVt9m261751pbEi3D0e2Sl2bltoMTffD-SFkrq2t6R_2IQ6psU0TnidPat0E-2L_HiVfPry_Ka6y1fpyWZyvMpPjnGdKIWSIlXVOiEAlrhBhmuLSakpwRSrJma44Y5woYbDikUKZG06txQYZJuhR8mryje3uBxt6uHNhbKBb2w0BCBcYM6yicDMJje9C8LaGrXd32u8AIxiZA4zMYeQHIz-YmIMgQCAyB4jMYc8cKCAo1vGniM4v9xV0MLqpvW6NCwd7QqlkYizwdZL9dI3d_ZX-n_B_Z_9ZRfNsMneht78O5tp_By6oYLD5dAkRplptiiuQ9Dd4QbGA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>26711519</pqid></control><display><type>article</type><title>Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells</title><source>Access via Wiley Online Library</source><creator>Cruz, Pedro E. ; Peixoto, Cristina C. ; Moreira, José L. ; Carrondo, Manuel J. T.</creator><creatorcontrib>Cruz, Pedro E. ; Peixoto, Cristina C. ; Moreira, José L. ; Carrondo, Manuel J. T.</creatorcontrib><description>In this work Sf‐9 cells previously adapted to SF900II and EXCELL 401 serum‐free media (SFM) were grown and infected at different agitation rates in order to study the effects of power input upon cell growth, infection and production of Pr55gag particles in both SFM. Maximum cell concentration increased from 3×106 to 6×106 cells cm−3 and 6·5×106 cells cm−3 when the agitation increased from 80 to 250 rpm or sparged aeration (0·01 vvm at 170 rpm) is used, clearly indicating that cell growth is limited by gas transfer. The specific productivity increased 3·5‐fold when the agitation rate was increased from 80 to 170 rpm, indicating that in SFM cell infection is also limited by gas transfer. The highest product concentration was obtained in SF900II at 120 h post‐infection (hpi). The product quality analysis showed that SF900II is the best medium for production of Pr55gag particles and that a careful optimisation of the harvest time is required. The maximum product titre was obtained at 120 hpi, 48 h after the achievement of the highest quality. © 1998 SCI</description><identifier>ISSN: 0268-2575</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/(SICI)1097-4660(199806)72:2<149::AID-JCTB886>3.0.CO;2-C</identifier><identifier>CODEN: JCTBDC</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animal cells ; Biological and medical sciences ; Biotechnology ; Establishment of new cell lines, improvement of cultural methods, mass cultures ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; insect cells ; Methods. Procedures. Technologies ; Pr55gag ; quality analysis ; serum-free medium ; virus-like particles</subject><ispartof>Journal of chemical technology and biotechnology (1986), 1998-06, Vol.72 (2), p.149-158</ispartof><rights>Copyright © 1998 Society of Chemical Industry</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291097-4660%28199806%2972%3A2%3C149%3A%3AAID-JCTB886%3E3.0.CO%3B2-C$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291097-4660%28199806%2972%3A2%3C149%3A%3AAID-JCTB886%3E3.0.CO%3B2-C$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2338579$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Cruz, Pedro E.</creatorcontrib><creatorcontrib>Peixoto, Cristina C.</creatorcontrib><creatorcontrib>Moreira, José L.</creatorcontrib><creatorcontrib>Carrondo, Manuel J. T.</creatorcontrib><title>Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells</title><title>Journal of chemical technology and biotechnology (1986)</title><addtitle>J. Chem. Technol. Biotechnol</addtitle><description>In this work Sf‐9 cells previously adapted to SF900II and EXCELL 401 serum‐free media (SFM) were grown and infected at different agitation rates in order to study the effects of power input upon cell growth, infection and production of Pr55gag particles in both SFM. Maximum cell concentration increased from 3×106 to 6×106 cells cm−3 and 6·5×106 cells cm−3 when the agitation increased from 80 to 250 rpm or sparged aeration (0·01 vvm at 170 rpm) is used, clearly indicating that cell growth is limited by gas transfer. The specific productivity increased 3·5‐fold when the agitation rate was increased from 80 to 170 rpm, indicating that in SFM cell infection is also limited by gas transfer. The highest product concentration was obtained in SF900II at 120 h post‐infection (hpi). The product quality analysis showed that SF900II is the best medium for production of Pr55gag particles and that a careful optimisation of the harvest time is required. The maximum product titre was obtained at 120 hpi, 48 h after the achievement of the highest quality. © 1998 SCI</description><subject>Animal cells</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>insect cells</subject><subject>Methods. Procedures. Technologies</subject><subject>Pr55gag</subject><subject>quality analysis</subject><subject>serum-free medium</subject><subject>virus-like particles</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkN1v0zAUxSMEEmXwP-QBoe0hnT_ir4KGRgZbUUUnMVSJlyvHcSZDlnR2AvS_x1lKX0DiyVfX555z9EuStxjNMULk9PjzslieYKRElnOOjrFSEvETQRbkDc7VYnG-vMg-FjfvpORndI7mxfo1yYpHyexw8ziZIcJlRphgT5NnIXxDCHFJ-Cwpr31XDaZ3XZvqtkrvB924fhdn3eyCC2lXp9eesVt9m261751pbEi3D0e2Sl2bltoMTffD-SFkrq2t6R_2IQ6psU0TnidPat0E-2L_HiVfPry_Ka6y1fpyWZyvMpPjnGdKIWSIlXVOiEAlrhBhmuLSakpwRSrJma44Y5woYbDikUKZG06txQYZJuhR8mryje3uBxt6uHNhbKBb2w0BCBcYM6yicDMJje9C8LaGrXd32u8AIxiZA4zMYeQHIz-YmIMgQCAyB4jMYc8cKCAo1vGniM4v9xV0MLqpvW6NCwd7QqlkYizwdZL9dI3d_ZX-n_B_Z_9ZRfNsMneht78O5tp_By6oYLD5dAkRplptiiuQ9Dd4QbGA</recordid><startdate>199806</startdate><enddate>199806</enddate><creator>Cruz, Pedro E.</creator><creator>Peixoto, Cristina C.</creator><creator>Moreira, José L.</creator><creator>Carrondo, Manuel J. T.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>199806</creationdate><title>Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells</title><author>Cruz, Pedro E. ; Peixoto, Cristina C. ; Moreira, José L. ; Carrondo, Manuel J. T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4146-9900c2e8f42270b1d025a31bea321d2d865ad6556297c196980b4c63ee1c0c573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animal cells</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>insect cells</topic><topic>Methods. Procedures. Technologies</topic><topic>Pr55gag</topic><topic>quality analysis</topic><topic>serum-free medium</topic><topic>virus-like particles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cruz, Pedro E.</creatorcontrib><creatorcontrib>Peixoto, Cristina C.</creatorcontrib><creatorcontrib>Moreira, José L.</creatorcontrib><creatorcontrib>Carrondo, Manuel J. T.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cruz, Pedro E.</au><au>Peixoto, Cristina C.</au><au>Moreira, José L.</au><au>Carrondo, Manuel J. T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><addtitle>J. Chem. Technol. Biotechnol</addtitle><date>1998-06</date><risdate>1998</risdate><volume>72</volume><issue>2</issue><spage>149</spage><epage>158</epage><pages>149-158</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><coden>JCTBDC</coden><abstract>In this work Sf‐9 cells previously adapted to SF900II and EXCELL 401 serum‐free media (SFM) were grown and infected at different agitation rates in order to study the effects of power input upon cell growth, infection and production of Pr55gag particles in both SFM. Maximum cell concentration increased from 3×106 to 6×106 cells cm−3 and 6·5×106 cells cm−3 when the agitation increased from 80 to 250 rpm or sparged aeration (0·01 vvm at 170 rpm) is used, clearly indicating that cell growth is limited by gas transfer. The specific productivity increased 3·5‐fold when the agitation rate was increased from 80 to 170 rpm, indicating that in SFM cell infection is also limited by gas transfer. The highest product concentration was obtained in SF900II at 120 h post‐infection (hpi). The product quality analysis showed that SF900II is the best medium for production of Pr55gag particles and that a careful optimisation of the harvest time is required. The maximum product titre was obtained at 120 hpi, 48 h after the achievement of the highest quality. © 1998 SCI</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/(SICI)1097-4660(199806)72:2<149::AID-JCTB886>3.0.CO;2-C</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-2575 |
| ispartof | Journal of chemical technology and biotechnology (1986), 1998-06, Vol.72 (2), p.149-158 |
| issn | 0268-2575 1097-4660 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_26711519 |
| source | Access via Wiley Online Library |
| subjects | Animal cells Biological and medical sciences Biotechnology Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology insect cells Methods. Procedures. Technologies Pr55gag quality analysis serum-free medium virus-like particles |
| title | Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T16%3A22%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Production%20and%20quality%20analysis%20of%20Pr55gag%20particles%20produced%20in%20baculovirus-infected%20insect%20cells&rft.jtitle=Journal%20of%20chemical%20technology%20and%20biotechnology%20(1986)&rft.au=Cruz,%20Pedro%20E.&rft.date=1998-06&rft.volume=72&rft.issue=2&rft.spage=149&rft.epage=158&rft.pages=149-158&rft.issn=0268-2575&rft.eissn=1097-4660&rft.coden=JCTBDC&rft_id=info:doi/10.1002/(SICI)1097-4660(199806)72:2%3C149::AID-JCTB886%3E3.0.CO;2-C&rft_dat=%3Cproquest_cross%3E26711519%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=26711519&rft_id=info:pmid/&rfr_iscdi=true |