Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats
Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-o...
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| Veröffentlicht in: | Microscopy and microanalysis 2022-10, Vol.28 (5), p.1687-1695 |
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| creator | Filipović, Branko Ajdžanović, Vladimir Živanović, Jasmina Trifunović, Svetlana Ristić, Nataša Milošević, Verica Šošić-Jurjević, Branka |
| description | Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level. |
| doi_str_mv | 10.1017/S1431927622000721 |
| format | Article |
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Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.]]></description><identifier>ISSN: 1431-9276</identifier><identifier>EISSN: 1435-8115</identifier><identifier>DOI: 10.1017/S1431927622000721</identifier><identifier>PMID: 35592886</identifier><language>eng</language><publisher>New York, USA: Cambridge University Press</publisher><subject>Aging ; Antibodies ; Biological Applications ; Biological research ; Bone resorption ; Calcitonin ; Calcium ions ; Hormones ; Hyperplasia ; Laboratory animals ; Localization ; Males ; Microscopy ; Middle age ; Mutation ; Peroxidase ; Phosphorus ; Physiology ; Rodents ; Secretory vesicles ; Software packages ; Statistical analysis ; Structure-function relationships ; Testosterone ; Thyroid ; Thyroid cancer ; Thyroid carcinoma ; Thyroid gland ; Variance analysis ; Young adults</subject><ispartof>Microscopy and microanalysis, 2022-10, Vol.28 (5), p.1687-1695</ispartof><rights>Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c368t-d26f1a080236fbcdf7b555a639161cac609559b634f1bb8bb19596b366fdb0f43</cites><orcidid>0000-0001-5352-763X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35592886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filipović, Branko</creatorcontrib><creatorcontrib>Ajdžanović, Vladimir</creatorcontrib><creatorcontrib>Živanović, Jasmina</creatorcontrib><creatorcontrib>Trifunović, Svetlana</creatorcontrib><creatorcontrib>Ristić, Nataša</creatorcontrib><creatorcontrib>Milošević, Verica</creatorcontrib><creatorcontrib>Šošić-Jurjević, Branka</creatorcontrib><title>Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats</title><title>Microscopy and microanalysis</title><addtitle>Microsc Microanal</addtitle><description><![CDATA[Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.]]></description><subject>Aging</subject><subject>Antibodies</subject><subject>Biological Applications</subject><subject>Biological research</subject><subject>Bone resorption</subject><subject>Calcitonin</subject><subject>Calcium ions</subject><subject>Hormones</subject><subject>Hyperplasia</subject><subject>Laboratory animals</subject><subject>Localization</subject><subject>Males</subject><subject>Microscopy</subject><subject>Middle age</subject><subject>Mutation</subject><subject>Peroxidase</subject><subject>Phosphorus</subject><subject>Physiology</subject><subject>Rodents</subject><subject>Secretory vesicles</subject><subject>Software packages</subject><subject>Statistical analysis</subject><subject>Structure-function relationships</subject><subject>Testosterone</subject><subject>Thyroid</subject><subject>Thyroid cancer</subject><subject>Thyroid carcinoma</subject><subject>Thyroid gland</subject><subject>Variance analysis</subject><subject>Young adults</subject><issn>1431-9276</issn><issn>1435-8115</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kE1LxDAQhoMofqz-AC9S8OKlmkk20-a4FL9AUVbFY0naZM3SbdekPfjvje6qoHiaYeaZd15eQg6BngKF7OwBxhwky5AxSmnGYIPsxpFIcwCx-dlD-rHfIXshzCPDaYbbZIcLIVme4y65m8xMOjWN6k2dFC-qnZmQuDYpVFO5vmtdm977rh4q186Sx5c337nIpYVpmpB0NrlVjUmeXeiVT6aqD_tky6ommIN1HZGni_PH4iq9ubu8LiY3acUx79OaoQVFc8o4Wl3VNtNCCIVcAkKlKqQyWtTIxxa0zrUGKSRqjmhrTe2Yj8jJSnfpu9fBhL5cuFBFV6o13RBKhphluZSCR_T4FzrvBt9GdyXLALnIJdJIwYqqfBeCN7ZcerdQ_q0EWn6kXf5JO94crZUHvTD198VXvBHga1G10N7VM_Pz-3_ZdzDAhsw</recordid><startdate>20221001</startdate><enddate>20221001</enddate><creator>Filipović, Branko</creator><creator>Ajdžanović, Vladimir</creator><creator>Živanović, Jasmina</creator><creator>Trifunović, Svetlana</creator><creator>Ristić, 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Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats</title><author>Filipović, Branko ; Ajdžanović, Vladimir ; Živanović, Jasmina ; Trifunović, Svetlana ; Ristić, Nataša ; Milošević, Verica ; Šošić-Jurjević, Branka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-d26f1a080236fbcdf7b555a639161cac609559b634f1bb8bb19596b366fdb0f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aging</topic><topic>Antibodies</topic><topic>Biological Applications</topic><topic>Biological research</topic><topic>Bone resorption</topic><topic>Calcitonin</topic><topic>Calcium ions</topic><topic>Hormones</topic><topic>Hyperplasia</topic><topic>Laboratory animals</topic><topic>Localization</topic><topic>Males</topic><topic>Microscopy</topic><topic>Middle 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Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats</atitle><jtitle>Microscopy and microanalysis</jtitle><addtitle>Microsc Microanal</addtitle><date>2022-10-01</date><risdate>2022</risdate><volume>28</volume><issue>5</issue><spage>1687</spage><epage>1695</epage><pages>1687-1695</pages><issn>1431-9276</issn><eissn>1435-8115</eissn><abstract><![CDATA[Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.]]></abstract><cop>New York, USA</cop><pub>Cambridge University Press</pub><pmid>35592886</pmid><doi>10.1017/S1431927622000721</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-5352-763X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Aging Antibodies Biological Applications Biological research Bone resorption Calcitonin Calcium ions Hormones Hyperplasia Laboratory animals Localization Males Microscopy Middle age Mutation Peroxidase Phosphorus Physiology Rodents Secretory vesicles Software packages Statistical analysis Structure-function relationships Testosterone Thyroid Thyroid cancer Thyroid carcinoma Thyroid gland Variance analysis Young adults |
| title | Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats |
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