Screening of hypoglycemic components in Platycladi Cacumen by phytochemical investigation, spectrum‐effect relationship, and chemometric methods
In this work, the hypoglycemic components in Platycladi Cacumen, an essential traditional Chinese medicine, were evaluated by combining phytochemical investigation, spectrum‐effect relationship analysis, and chemometric methods. The phytochemical studies on Platycladi Cacumen extract lead to the iso...
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| Veröffentlicht in: | Journal of separation science 2022-07, Vol.45 (14), p.2591-2602 |
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| creator | Duan, Peng‐Bo Xiao, Ping‐Ting Yang, Xing Hao, Jin‐Hua Li, Kai Liu, E‐Hu |
| description | In this work, the hypoglycemic components in Platycladi Cacumen, an essential traditional Chinese medicine, were evaluated by combining phytochemical investigation, spectrum‐effect relationship analysis, and chemometric methods. The phytochemical studies on Platycladi Cacumen extract lead to the isolation of 21 potential bioactive compounds. The chromatographic fingerprints of Platycladi Cacumen samples were established by high‐performance liquid chromatography. The hypoglycemic effects of Platycladi Cacumen samples were further evaluated by inhibition of α‐glucosidase and detected by the high‐performance liquid chromatography method. The spectrum‐effect relationship study by bivariate correlations analysis and orthogonal partial least squares regression revealed that myricitrin (P9), quercitrin (P13), afzelin (P18), and amentoflavone (P24) were more relevant to the α‐glucosidase inhibitory activity. The results of α‐glucosidase inhibitory activity of 21 isolated compounds and molecular docking studies also indicated these flavonoids had potent α‐glucosidase inhibitory activity. Collectively, the present study established the spectrum‐effect relationship mode of Platycladi Cacumen and discovered the major hypoglycemic components, which provides a feasible method for screening bioactive components. |
| doi_str_mv | 10.1002/jssc.202200221 |
| format | Article |
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The phytochemical studies on Platycladi Cacumen extract lead to the isolation of 21 potential bioactive compounds. The chromatographic fingerprints of Platycladi Cacumen samples were established by high‐performance liquid chromatography. The hypoglycemic effects of Platycladi Cacumen samples were further evaluated by inhibition of α‐glucosidase and detected by the high‐performance liquid chromatography method. The spectrum‐effect relationship study by bivariate correlations analysis and orthogonal partial least squares regression revealed that myricitrin (P9), quercitrin (P13), afzelin (P18), and amentoflavone (P24) were more relevant to the α‐glucosidase inhibitory activity. The results of α‐glucosidase inhibitory activity of 21 isolated compounds and molecular docking studies also indicated these flavonoids had potent α‐glucosidase inhibitory activity. Collectively, the present study established the spectrum‐effect relationship mode of Platycladi Cacumen and discovered the major hypoglycemic components, which provides a feasible method for screening bioactive components.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.202200221</identifier><identifier>PMID: 35593082</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Biological activity ; Bivariate analysis ; chemometric methods ; Chemometrics ; Chromatography ; Flavonoids ; Glucosidase ; Hypoglycemia ; hypoglycemic activity ; Least squares method ; Liquid chromatography ; Molecular docking ; Phytochemicals ; Platycladi Cacumen ; Screening ; spectrum‐effect relationship ; Traditional Chinese medicine</subject><ispartof>Journal of separation science, 2022-07, Vol.45 (14), p.2591-2602</ispartof><rights>2022 Wiley‐VCH GmbH.</rights><rights>2022 Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2985-eb1c32a3166c757741fae23c2faa7eaf38aee0a146d3e29bb51e5ee2c1ea30463</citedby><cites>FETCH-LOGICAL-c2985-eb1c32a3166c757741fae23c2faa7eaf38aee0a146d3e29bb51e5ee2c1ea30463</cites><orcidid>0000-0002-7281-210X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.202200221$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.202200221$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27928,27929,45578,45579</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35593082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duan, Peng‐Bo</creatorcontrib><creatorcontrib>Xiao, Ping‐Ting</creatorcontrib><creatorcontrib>Yang, Xing</creatorcontrib><creatorcontrib>Hao, Jin‐Hua</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Liu, E‐Hu</creatorcontrib><title>Screening of hypoglycemic components in Platycladi Cacumen by phytochemical investigation, spectrum‐effect relationship, and chemometric methods</title><title>Journal of separation science</title><addtitle>J Sep Sci</addtitle><description>In this work, the hypoglycemic components in Platycladi Cacumen, an essential traditional Chinese medicine, were evaluated by combining phytochemical investigation, spectrum‐effect relationship analysis, and chemometric methods. The phytochemical studies on Platycladi Cacumen extract lead to the isolation of 21 potential bioactive compounds. The chromatographic fingerprints of Platycladi Cacumen samples were established by high‐performance liquid chromatography. The hypoglycemic effects of Platycladi Cacumen samples were further evaluated by inhibition of α‐glucosidase and detected by the high‐performance liquid chromatography method. The spectrum‐effect relationship study by bivariate correlations analysis and orthogonal partial least squares regression revealed that myricitrin (P9), quercitrin (P13), afzelin (P18), and amentoflavone (P24) were more relevant to the α‐glucosidase inhibitory activity. The results of α‐glucosidase inhibitory activity of 21 isolated compounds and molecular docking studies also indicated these flavonoids had potent α‐glucosidase inhibitory activity. Collectively, the present study established the spectrum‐effect relationship mode of Platycladi Cacumen and discovered the major hypoglycemic components, which provides a feasible method for screening bioactive components.</description><subject>Biological activity</subject><subject>Bivariate analysis</subject><subject>chemometric methods</subject><subject>Chemometrics</subject><subject>Chromatography</subject><subject>Flavonoids</subject><subject>Glucosidase</subject><subject>Hypoglycemia</subject><subject>hypoglycemic activity</subject><subject>Least squares method</subject><subject>Liquid chromatography</subject><subject>Molecular docking</subject><subject>Phytochemicals</subject><subject>Platycladi Cacumen</subject><subject>Screening</subject><subject>spectrum‐effect relationship</subject><subject>Traditional Chinese medicine</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u1DAUhS1URH9gyxJZYsOiM_gntifLagQUVAmkgXXkODcTjxw7tZOi7HgExCP2SephyizYdHWv7e-ee-SD0GtKlpQQ9n6Xklkywlg-MPoMnVFJxaLktDg59kSeovOUdoRQtSrJC3TKhcjXK3aG_mxMBPDWb3FocTcPYetmA7012IR-CB78mLD1-JvT42ycbixeazP14HE946Gbx2C6Pa9dxu4gjXarRxv8JU4DmDFO_f2v39C2uccR3N-31NnhEmvf4P1s6GGMeWEuXWjSS_S81S7Bq8d6gX58_PB9fb24-frp8_rqZmFYuRILqKnhTHMqpVFCqYK2Ghg3rNVagW75SgMQTQvZcGBlXQsKAoAZCpqTQvIL9O6gO8RwO2XjVW-TAee0hzClikmp8n9JRTP69j90F6bos7tMlUIoKSTP1PJAmRhSitBWQ7S9jnNFSbVPq9qnVR3TygNvHmWnuofmiP-LJwPFAfhpHcxPyFVfNpu1ynb4Axq5pWk</recordid><startdate>202207</startdate><enddate>202207</enddate><creator>Duan, Peng‐Bo</creator><creator>Xiao, Ping‐Ting</creator><creator>Yang, Xing</creator><creator>Hao, Jin‐Hua</creator><creator>Li, Kai</creator><creator>Liu, E‐Hu</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7281-210X</orcidid></search><sort><creationdate>202207</creationdate><title>Screening of hypoglycemic components in Platycladi Cacumen by phytochemical investigation, spectrum‐effect relationship, and chemometric methods</title><author>Duan, Peng‐Bo ; Xiao, Ping‐Ting ; Yang, Xing ; Hao, Jin‐Hua ; Li, Kai ; Liu, E‐Hu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2985-eb1c32a3166c757741fae23c2faa7eaf38aee0a146d3e29bb51e5ee2c1ea30463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biological activity</topic><topic>Bivariate analysis</topic><topic>chemometric methods</topic><topic>Chemometrics</topic><topic>Chromatography</topic><topic>Flavonoids</topic><topic>Glucosidase</topic><topic>Hypoglycemia</topic><topic>hypoglycemic activity</topic><topic>Least squares method</topic><topic>Liquid chromatography</topic><topic>Molecular docking</topic><topic>Phytochemicals</topic><topic>Platycladi Cacumen</topic><topic>Screening</topic><topic>spectrum‐effect relationship</topic><topic>Traditional Chinese medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duan, Peng‐Bo</creatorcontrib><creatorcontrib>Xiao, Ping‐Ting</creatorcontrib><creatorcontrib>Yang, Xing</creatorcontrib><creatorcontrib>Hao, Jin‐Hua</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Liu, E‐Hu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duan, Peng‐Bo</au><au>Xiao, Ping‐Ting</au><au>Yang, Xing</au><au>Hao, Jin‐Hua</au><au>Li, Kai</au><au>Liu, E‐Hu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of hypoglycemic components in Platycladi Cacumen by phytochemical investigation, spectrum‐effect relationship, and chemometric methods</atitle><jtitle>Journal of separation science</jtitle><addtitle>J Sep Sci</addtitle><date>2022-07</date><risdate>2022</risdate><volume>45</volume><issue>14</issue><spage>2591</spage><epage>2602</epage><pages>2591-2602</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>In this work, the hypoglycemic components in Platycladi Cacumen, an essential traditional Chinese medicine, were evaluated by combining phytochemical investigation, spectrum‐effect relationship analysis, and chemometric methods. The phytochemical studies on Platycladi Cacumen extract lead to the isolation of 21 potential bioactive compounds. The chromatographic fingerprints of Platycladi Cacumen samples were established by high‐performance liquid chromatography. The hypoglycemic effects of Platycladi Cacumen samples were further evaluated by inhibition of α‐glucosidase and detected by the high‐performance liquid chromatography method. The spectrum‐effect relationship study by bivariate correlations analysis and orthogonal partial least squares regression revealed that myricitrin (P9), quercitrin (P13), afzelin (P18), and amentoflavone (P24) were more relevant to the α‐glucosidase inhibitory activity. The results of α‐glucosidase inhibitory activity of 21 isolated compounds and molecular docking studies also indicated these flavonoids had potent α‐glucosidase inhibitory activity. Collectively, the present study established the spectrum‐effect relationship mode of Platycladi Cacumen and discovered the major hypoglycemic components, which provides a feasible method for screening bioactive components.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35593082</pmid><doi>10.1002/jssc.202200221</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-7281-210X</orcidid></addata></record> |
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| subjects | Biological activity Bivariate analysis chemometric methods Chemometrics Chromatography Flavonoids Glucosidase Hypoglycemia hypoglycemic activity Least squares method Liquid chromatography Molecular docking Phytochemicals Platycladi Cacumen Screening spectrum‐effect relationship Traditional Chinese medicine |
| title | Screening of hypoglycemic components in Platycladi Cacumen by phytochemical investigation, spectrum‐effect relationship, and chemometric methods |
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