Determination of intracellular ascorbic acid using tandem mass spectrometry
Ascorbic acid is involved in a variety of biological events. Nevertheless, its detailed intracellular behavior remains unexplored due to a lack of sensitive analytical methods. Here we report a method using HPLC-ESI-MS/MS achieving the lowest detection limit ever reported (1 pg), to provide mechanis...
Gespeichert in:
| Veröffentlicht in: | Analyst (London) 2022-06, Vol.147 (12), p.264-2643 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2643 |
|---|---|
| container_issue | 12 |
| container_start_page | 264 |
| container_title | Analyst (London) |
| container_volume | 147 |
| creator | Abe, Chizumi Higuchi, Ohki Matsumoto, Akira Miyazawa, Taiki |
| description | Ascorbic acid is involved in a variety of biological events. Nevertheless, its detailed intracellular behavior remains unexplored due to a lack of sensitive analytical methods. Here we report a method using HPLC-ESI-MS/MS achieving the lowest detection limit ever reported (1 pg), to provide mechanistic insight into the intracellular ascorbic acid physiology.
Ascorbic acid is involved in a variety of biological events. |
| doi_str_mv | 10.1039/d1an02160e |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2667785482</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2667785482</sourcerecordid><originalsourceid>FETCH-LOGICAL-c337t-6b9f0c7675050aa1c1d982c731b8d4e6e77332af1c3d6fccbdd9aabc1ee1c073</originalsourceid><addsrcrecordid>eNpd0c1LwzAYBvAgipvTi3cl4EWEatI0SXsc2_zAoZfdS5qkEmmTmaSH_fdmbk7w9BLeHy8PTwC4xOgeI1I9KCwsyjFD-giMMWFFRmleHoMxQohkOaPFCJyF8JmeGFF0CkaE0orwCo_B61xH7XtjRTTOQtdCY6MXUnfd0AkPRZDON0ZCIY2CQzD2A0Zhle5hL0KAYa1l9K7X0W_OwUkruqAv9nMCVo-L1ew5W74_vcymy0wSwmPGmqpFkjNOUxghsMSqKnPJCW5KVWimOSckFy2WRLFWykapSohGYq2xRJxMwO3u7Nq7r0GHWPcmbAMLq90Q6pwxzktalHmiN__opxu8TeGS4qklwilJ6m6npHcheN3Wa2964Tc1RvW24XqOp28_DS8Svt6fHJpeqwP9rTSBqx3wQR62f19EvgGw5YCK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2675523753</pqid></control><display><type>article</type><title>Determination of intracellular ascorbic acid using tandem mass spectrometry</title><source>MEDLINE</source><source>Royal Society of Chemistry Journals Archive (1841-2007)</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Abe, Chizumi ; Higuchi, Ohki ; Matsumoto, Akira ; Miyazawa, Taiki</creator><creatorcontrib>Abe, Chizumi ; Higuchi, Ohki ; Matsumoto, Akira ; Miyazawa, Taiki</creatorcontrib><description>Ascorbic acid is involved in a variety of biological events. Nevertheless, its detailed intracellular behavior remains unexplored due to a lack of sensitive analytical methods. Here we report a method using HPLC-ESI-MS/MS achieving the lowest detection limit ever reported (1 pg), to provide mechanistic insight into the intracellular ascorbic acid physiology.
Ascorbic acid is involved in a variety of biological events.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/d1an02160e</identifier><identifier>PMID: 35593791</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Ascorbic Acid ; Chromatography, High Pressure Liquid - methods ; Mass spectrometry ; Spectrometry, Mass, Electrospray Ionization - methods ; Tandem Mass Spectrometry - methods</subject><ispartof>Analyst (London), 2022-06, Vol.147 (12), p.264-2643</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-6b9f0c7675050aa1c1d982c731b8d4e6e77332af1c3d6fccbdd9aabc1ee1c073</citedby><cites>FETCH-LOGICAL-c337t-6b9f0c7675050aa1c1d982c731b8d4e6e77332af1c3d6fccbdd9aabc1ee1c073</cites><orcidid>0000-0002-8937-5110 ; 0000-0002-7568-8677 ; 0000-0002-3955-9907</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,2832,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35593791$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abe, Chizumi</creatorcontrib><creatorcontrib>Higuchi, Ohki</creatorcontrib><creatorcontrib>Matsumoto, Akira</creatorcontrib><creatorcontrib>Miyazawa, Taiki</creatorcontrib><title>Determination of intracellular ascorbic acid using tandem mass spectrometry</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>Ascorbic acid is involved in a variety of biological events. Nevertheless, its detailed intracellular behavior remains unexplored due to a lack of sensitive analytical methods. Here we report a method using HPLC-ESI-MS/MS achieving the lowest detection limit ever reported (1 pg), to provide mechanistic insight into the intracellular ascorbic acid physiology.
Ascorbic acid is involved in a variety of biological events.</description><subject>Ascorbic Acid</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Mass spectrometry</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0c1LwzAYBvAgipvTi3cl4EWEatI0SXsc2_zAoZfdS5qkEmmTmaSH_fdmbk7w9BLeHy8PTwC4xOgeI1I9KCwsyjFD-giMMWFFRmleHoMxQohkOaPFCJyF8JmeGFF0CkaE0orwCo_B61xH7XtjRTTOQtdCY6MXUnfd0AkPRZDON0ZCIY2CQzD2A0Zhle5hL0KAYa1l9K7X0W_OwUkruqAv9nMCVo-L1ew5W74_vcymy0wSwmPGmqpFkjNOUxghsMSqKnPJCW5KVWimOSckFy2WRLFWykapSohGYq2xRJxMwO3u7Nq7r0GHWPcmbAMLq90Q6pwxzktalHmiN__opxu8TeGS4qklwilJ6m6npHcheN3Wa2964Tc1RvW24XqOp28_DS8Svt6fHJpeqwP9rTSBqx3wQR62f19EvgGw5YCK</recordid><startdate>20220613</startdate><enddate>20220613</enddate><creator>Abe, Chizumi</creator><creator>Higuchi, Ohki</creator><creator>Matsumoto, Akira</creator><creator>Miyazawa, Taiki</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8937-5110</orcidid><orcidid>https://orcid.org/0000-0002-7568-8677</orcidid><orcidid>https://orcid.org/0000-0002-3955-9907</orcidid></search><sort><creationdate>20220613</creationdate><title>Determination of intracellular ascorbic acid using tandem mass spectrometry</title><author>Abe, Chizumi ; Higuchi, Ohki ; Matsumoto, Akira ; Miyazawa, Taiki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-6b9f0c7675050aa1c1d982c731b8d4e6e77332af1c3d6fccbdd9aabc1ee1c073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Ascorbic Acid</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Mass spectrometry</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abe, Chizumi</creatorcontrib><creatorcontrib>Higuchi, Ohki</creatorcontrib><creatorcontrib>Matsumoto, Akira</creatorcontrib><creatorcontrib>Miyazawa, Taiki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abe, Chizumi</au><au>Higuchi, Ohki</au><au>Matsumoto, Akira</au><au>Miyazawa, Taiki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of intracellular ascorbic acid using tandem mass spectrometry</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2022-06-13</date><risdate>2022</risdate><volume>147</volume><issue>12</issue><spage>264</spage><epage>2643</epage><pages>264-2643</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>Ascorbic acid is involved in a variety of biological events. Nevertheless, its detailed intracellular behavior remains unexplored due to a lack of sensitive analytical methods. Here we report a method using HPLC-ESI-MS/MS achieving the lowest detection limit ever reported (1 pg), to provide mechanistic insight into the intracellular ascorbic acid physiology.
Ascorbic acid is involved in a variety of biological events.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35593791</pmid><doi>10.1039/d1an02160e</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-8937-5110</orcidid><orcidid>https://orcid.org/0000-0002-7568-8677</orcidid><orcidid>https://orcid.org/0000-0002-3955-9907</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2654 |
| ispartof | Analyst (London), 2022-06, Vol.147 (12), p.264-2643 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2667785482 |
| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Ascorbic Acid Chromatography, High Pressure Liquid - methods Mass spectrometry Spectrometry, Mass, Electrospray Ionization - methods Tandem Mass Spectrometry - methods |
| title | Determination of intracellular ascorbic acid using tandem mass spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T03%3A05%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20intracellular%20ascorbic%20acid%20using%20tandem%20mass%20spectrometry&rft.jtitle=Analyst%20(London)&rft.au=Abe,%20Chizumi&rft.date=2022-06-13&rft.volume=147&rft.issue=12&rft.spage=264&rft.epage=2643&rft.pages=264-2643&rft.issn=0003-2654&rft.eissn=1364-5528&rft_id=info:doi/10.1039/d1an02160e&rft_dat=%3Cproquest_cross%3E2667785482%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2675523753&rft_id=info:pmid/35593791&rfr_iscdi=true |