HPLC -DAD analysis, anti-inflammatory and anti-arthritic potentials of Coronopus didymus (L.) Sm. extracts: effects on pro- and anti-inflammatory cytokines, COX-2, I-κβ, NF-κβ and oxidative stress biomarkers
(CD) has been traditionally used to treat pain, rheumatism, and inflammation. This study was planned to appraise the anti-oxidant, anti-inflammatory and anti-arthritic potentials of CD (whole plant) aqueous ethanolic (CDAEE) and aqueous extracts (CDAE) and chemical characterization by high-performan...
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| Veröffentlicht in: | Food & function 2022-06, Vol.13 (11), p.6244-6258 |
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| creator | Saleem, Ammara Khalid, Huma Akhtar, Muhammad Furqan Zeb, Alam |
| description | (CD) has been traditionally used to treat pain, rheumatism, and inflammation. This study was planned to appraise the anti-oxidant, anti-inflammatory and anti-arthritic potentials of CD (whole plant) aqueous ethanolic (CDAEE) and aqueous extracts (CDAE) and chemical characterization by high-performance liquid chromatography-diode array detector.
anti-inflammatory (Carrageenan induced paw edema, and Xylene induced ear edema assays) and anti-arthritic potentials were evaluated in Wistar rats. Both extracts showed significant (
< 0.0001)
free radical scavenging and
anti-arthritic potentials by inhibition of protein denaturation and stabilization of the HRBC membrane and anti-oedematogenic potential, whereas more activity was expressed by CDAEE. In complete Freund's adjuvant-induced arthritic model, the CDAEE at 200, 400, and 800 mg kg
and methotrexate (1 mg kg
) profoundly (
< 0.05) reduced the arthritic score and paw edema, restored body and immune organ weight, and altered blood parameters and oxidative stress biomarkers. The qRT-PCR analysis revealed that CDAEE at 400 mg kg
significantly (
< 0.0001) downregulated TNF-α (2.22 ± 0.16 fold), IL-6 (2.29 ± 0.05 fold), IL-1β (2.10 ± 0.01 fold), COX-2 (2.45 ± 0.02 fold), and NF-ĸβ (2.72 ± 0.06 fold) and considerably upregulated IL-10 (58.84 ± 0.67%), IL-4 (76.16 ± 2.79%) and I-kβ (75.45 ± 0.17%) in arthritic rats in contrast to disease control and methotrexate as evidenced from the joint histology. These findings suggested the antioxidant, anti-inflammatory and anti-arthritic activities of
, which might be due to the presence of quercetin, ferulic acid, dihydromyricetin, apigenin, vitexin, and kaempferol in CDAEE. |
| doi_str_mv | 10.1039/d2fo00207h |
| format | Article |
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anti-inflammatory (Carrageenan induced paw edema, and Xylene induced ear edema assays) and anti-arthritic potentials were evaluated in Wistar rats. Both extracts showed significant (
< 0.0001)
free radical scavenging and
anti-arthritic potentials by inhibition of protein denaturation and stabilization of the HRBC membrane and anti-oedematogenic potential, whereas more activity was expressed by CDAEE. In complete Freund's adjuvant-induced arthritic model, the CDAEE at 200, 400, and 800 mg kg
and methotrexate (1 mg kg
) profoundly (
< 0.05) reduced the arthritic score and paw edema, restored body and immune organ weight, and altered blood parameters and oxidative stress biomarkers. The qRT-PCR analysis revealed that CDAEE at 400 mg kg
significantly (
< 0.0001) downregulated TNF-α (2.22 ± 0.16 fold), IL-6 (2.29 ± 0.05 fold), IL-1β (2.10 ± 0.01 fold), COX-2 (2.45 ± 0.02 fold), and NF-ĸβ (2.72 ± 0.06 fold) and considerably upregulated IL-10 (58.84 ± 0.67%), IL-4 (76.16 ± 2.79%) and I-kβ (75.45 ± 0.17%) in arthritic rats in contrast to disease control and methotrexate as evidenced from the joint histology. These findings suggested the antioxidant, anti-inflammatory and anti-arthritic activities of
, which might be due to the presence of quercetin, ferulic acid, dihydromyricetin, apigenin, vitexin, and kaempferol in CDAEE.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/d2fo00207h</identifier><identifier>PMID: 35587790</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Animal models ; Antioxidants ; Biomarkers ; Biopolymer denaturation ; Carrageenan ; Coronopus didymus ; Cyclooxygenase-2 ; Cytokines ; Disease control ; Edema ; Ferulic acid ; Free radicals ; Freund's adjuvant ; High performance liquid chromatography ; Histology ; IL-1β ; Inflammation ; Interleukin 10 ; Interleukin 4 ; Interleukin 6 ; Kaempferol ; Liquid chromatography ; Methotrexate ; Organ weight ; Oxidants ; Oxidative stress ; Oxidizing agents ; Pain ; Protein denaturation ; Quercetin ; Scavenging ; Tumor necrosis factor-α ; Xylene</subject><ispartof>Food & function, 2022-06, Vol.13 (11), p.6244-6258</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-76eaeb1e619165084bc8902efe4c07c3554cdc656bcb8339a2c5f48f4f918b303</citedby><cites>FETCH-LOGICAL-c315t-76eaeb1e619165084bc8902efe4c07c3554cdc656bcb8339a2c5f48f4f918b303</cites><orcidid>0000-0002-2478-7800</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35587790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saleem, Ammara</creatorcontrib><creatorcontrib>Khalid, Huma</creatorcontrib><creatorcontrib>Akhtar, Muhammad Furqan</creatorcontrib><creatorcontrib>Zeb, Alam</creatorcontrib><title>HPLC -DAD analysis, anti-inflammatory and anti-arthritic potentials of Coronopus didymus (L.) Sm. extracts: effects on pro- and anti-inflammatory cytokines, COX-2, I-κβ, NF-κβ and oxidative stress biomarkers</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>(CD) has been traditionally used to treat pain, rheumatism, and inflammation. This study was planned to appraise the anti-oxidant, anti-inflammatory and anti-arthritic potentials of CD (whole plant) aqueous ethanolic (CDAEE) and aqueous extracts (CDAE) and chemical characterization by high-performance liquid chromatography-diode array detector.
anti-inflammatory (Carrageenan induced paw edema, and Xylene induced ear edema assays) and anti-arthritic potentials were evaluated in Wistar rats. Both extracts showed significant (
< 0.0001)
free radical scavenging and
anti-arthritic potentials by inhibition of protein denaturation and stabilization of the HRBC membrane and anti-oedematogenic potential, whereas more activity was expressed by CDAEE. In complete Freund's adjuvant-induced arthritic model, the CDAEE at 200, 400, and 800 mg kg
and methotrexate (1 mg kg
) profoundly (
< 0.05) reduced the arthritic score and paw edema, restored body and immune organ weight, and altered blood parameters and oxidative stress biomarkers. The qRT-PCR analysis revealed that CDAEE at 400 mg kg
significantly (
< 0.0001) downregulated TNF-α (2.22 ± 0.16 fold), IL-6 (2.29 ± 0.05 fold), IL-1β (2.10 ± 0.01 fold), COX-2 (2.45 ± 0.02 fold), and NF-ĸβ (2.72 ± 0.06 fold) and considerably upregulated IL-10 (58.84 ± 0.67%), IL-4 (76.16 ± 2.79%) and I-kβ (75.45 ± 0.17%) in arthritic rats in contrast to disease control and methotrexate as evidenced from the joint histology. These findings suggested the antioxidant, anti-inflammatory and anti-arthritic activities of
, which might be due to the presence of quercetin, ferulic acid, dihydromyricetin, apigenin, vitexin, and kaempferol in CDAEE.</description><subject>Animal models</subject><subject>Antioxidants</subject><subject>Biomarkers</subject><subject>Biopolymer denaturation</subject><subject>Carrageenan</subject><subject>Coronopus didymus</subject><subject>Cyclooxygenase-2</subject><subject>Cytokines</subject><subject>Disease control</subject><subject>Edema</subject><subject>Ferulic acid</subject><subject>Free radicals</subject><subject>Freund's adjuvant</subject><subject>High performance liquid chromatography</subject><subject>Histology</subject><subject>IL-1β</subject><subject>Inflammation</subject><subject>Interleukin 10</subject><subject>Interleukin 4</subject><subject>Interleukin 6</subject><subject>Kaempferol</subject><subject>Liquid chromatography</subject><subject>Methotrexate</subject><subject>Organ weight</subject><subject>Oxidants</subject><subject>Oxidative stress</subject><subject>Oxidizing agents</subject><subject>Pain</subject><subject>Protein denaturation</subject><subject>Quercetin</subject><subject>Scavenging</subject><subject>Tumor necrosis factor-α</subject><subject>Xylene</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpdkc1u1DAUhSMEolXphgdAltgUNBkcO3FsdlWmw1QaMUiA1F3kONeq2ySe2g5qXqtLHqIbXgh3pgWBN_fo6PP9TZLXGZ5nmIoPLdEWY4LLy2fJIcE5SVmBL54_6Vywg-TY-yscHxWCC_4yOaBFwctS4MPk1-rLukLp4nSB5CC7yRs_iyqY1Ay6k30vg3VTdNq9K124dCYYhbY2QHRk55HVqLLODnY7etSadupjPFnP36Gv_RzBbXBSBf8RgdYQBbID2jqb_s36Ty01BXttBoiNVJuLlMzQeXr_8_5uhj4vd2L3z96aVgbzA5APDrxHjbG9dNfg_KvkhY5twfFjPEq-L8--Vat0vfl0Xp2uU0WzIqQlAwlNBiwTWdwZzxvFBSagIVe4VHFHuWoVK1ijGk6pkEQVOuc61yLjDcX0KDnZ543D3IzgQ90br6Dr5AB29DVhrCx5QQSJ6Nv_0Cs7urjwB6qkNGeUsUi931PKWe8d6HrrTBxqqjNcP1y7XpDlZnftVYTfPKYcmx7aP-jTbelvOEyonQ</recordid><startdate>20220606</startdate><enddate>20220606</enddate><creator>Saleem, Ammara</creator><creator>Khalid, Huma</creator><creator>Akhtar, Muhammad Furqan</creator><creator>Zeb, Alam</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2478-7800</orcidid></search><sort><creationdate>20220606</creationdate><title>HPLC -DAD analysis, anti-inflammatory and anti-arthritic potentials of Coronopus didymus (L.) Sm. extracts: effects on pro- and anti-inflammatory cytokines, COX-2, I-κβ, NF-κβ and oxidative stress biomarkers</title><author>Saleem, Ammara ; Khalid, Huma ; Akhtar, Muhammad Furqan ; Zeb, Alam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-76eaeb1e619165084bc8902efe4c07c3554cdc656bcb8339a2c5f48f4f918b303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal models</topic><topic>Antioxidants</topic><topic>Biomarkers</topic><topic>Biopolymer denaturation</topic><topic>Carrageenan</topic><topic>Coronopus didymus</topic><topic>Cyclooxygenase-2</topic><topic>Cytokines</topic><topic>Disease control</topic><topic>Edema</topic><topic>Ferulic acid</topic><topic>Free radicals</topic><topic>Freund's adjuvant</topic><topic>High performance liquid chromatography</topic><topic>Histology</topic><topic>IL-1β</topic><topic>Inflammation</topic><topic>Interleukin 10</topic><topic>Interleukin 4</topic><topic>Interleukin 6</topic><topic>Kaempferol</topic><topic>Liquid chromatography</topic><topic>Methotrexate</topic><topic>Organ weight</topic><topic>Oxidants</topic><topic>Oxidative stress</topic><topic>Oxidizing agents</topic><topic>Pain</topic><topic>Protein denaturation</topic><topic>Quercetin</topic><topic>Scavenging</topic><topic>Tumor necrosis factor-α</topic><topic>Xylene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saleem, Ammara</creatorcontrib><creatorcontrib>Khalid, Huma</creatorcontrib><creatorcontrib>Akhtar, Muhammad Furqan</creatorcontrib><creatorcontrib>Zeb, Alam</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saleem, Ammara</au><au>Khalid, Huma</au><au>Akhtar, Muhammad Furqan</au><au>Zeb, Alam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HPLC -DAD analysis, anti-inflammatory and anti-arthritic potentials of Coronopus didymus (L.) Sm. extracts: effects on pro- and anti-inflammatory cytokines, COX-2, I-κβ, NF-κβ and oxidative stress biomarkers</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2022-06-06</date><risdate>2022</risdate><volume>13</volume><issue>11</issue><spage>6244</spage><epage>6258</epage><pages>6244-6258</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>(CD) has been traditionally used to treat pain, rheumatism, and inflammation. This study was planned to appraise the anti-oxidant, anti-inflammatory and anti-arthritic potentials of CD (whole plant) aqueous ethanolic (CDAEE) and aqueous extracts (CDAE) and chemical characterization by high-performance liquid chromatography-diode array detector.
anti-inflammatory (Carrageenan induced paw edema, and Xylene induced ear edema assays) and anti-arthritic potentials were evaluated in Wistar rats. Both extracts showed significant (
< 0.0001)
free radical scavenging and
anti-arthritic potentials by inhibition of protein denaturation and stabilization of the HRBC membrane and anti-oedematogenic potential, whereas more activity was expressed by CDAEE. In complete Freund's adjuvant-induced arthritic model, the CDAEE at 200, 400, and 800 mg kg
and methotrexate (1 mg kg
) profoundly (
< 0.05) reduced the arthritic score and paw edema, restored body and immune organ weight, and altered blood parameters and oxidative stress biomarkers. The qRT-PCR analysis revealed that CDAEE at 400 mg kg
significantly (
< 0.0001) downregulated TNF-α (2.22 ± 0.16 fold), IL-6 (2.29 ± 0.05 fold), IL-1β (2.10 ± 0.01 fold), COX-2 (2.45 ± 0.02 fold), and NF-ĸβ (2.72 ± 0.06 fold) and considerably upregulated IL-10 (58.84 ± 0.67%), IL-4 (76.16 ± 2.79%) and I-kβ (75.45 ± 0.17%) in arthritic rats in contrast to disease control and methotrexate as evidenced from the joint histology. These findings suggested the antioxidant, anti-inflammatory and anti-arthritic activities of
, which might be due to the presence of quercetin, ferulic acid, dihydromyricetin, apigenin, vitexin, and kaempferol in CDAEE.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35587790</pmid><doi>10.1039/d2fo00207h</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-2478-7800</orcidid></addata></record> |
| fulltext | fulltext |
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| issn | 2042-6496 2042-650X |
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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Animal models Antioxidants Biomarkers Biopolymer denaturation Carrageenan Coronopus didymus Cyclooxygenase-2 Cytokines Disease control Edema Ferulic acid Free radicals Freund's adjuvant High performance liquid chromatography Histology IL-1β Inflammation Interleukin 10 Interleukin 4 Interleukin 6 Kaempferol Liquid chromatography Methotrexate Organ weight Oxidants Oxidative stress Oxidizing agents Pain Protein denaturation Quercetin Scavenging Tumor necrosis factor-α Xylene |
| title | HPLC -DAD analysis, anti-inflammatory and anti-arthritic potentials of Coronopus didymus (L.) Sm. extracts: effects on pro- and anti-inflammatory cytokines, COX-2, I-κβ, NF-κβ and oxidative stress biomarkers |
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