SARS-CoV-2 Kappa Variant Shows Pathogenicity in a Syrian Hamster Model
Objectives: The emergence of SARS-CoV-2 lineage B.1.617 variants in India has been associated with a surge in the number of daily infections. We investigated the pathogenic potential of Kappa (B.1.617.1) variant in Syrian golden hamsters. Methods: Two groups of Syrian golden hamsters (18 each) were...
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| Veröffentlicht in: | Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2022-05, Vol.22 (5), p.289-296 |
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| creator | Yadav, Pragya D Mohandas, Sreelekshmy Shete, Anita M Nyayanit, Dimpal A Gupta, Nivedita Patil, Deepak Y Sapkal, Gajanan N Potdar, Varsha Kadam, Manoj Kumar, Abhimanyu Kumar, Sanjay Suryavanshi, Deepak Mote, Chandrashekhar S Abraham, Priya Panda, Samiran Bhargava, Balram |
| description | Objectives:
The emergence of SARS-CoV-2 lineage B.1.617 variants in India has been associated with a surge in the number of daily infections. We investigated the pathogenic potential of Kappa (B.1.617.1) variant in Syrian golden hamsters.
Methods:
Two groups of Syrian golden hamsters (18 each) were inoculated intranasally with SARS-CoV-2 isolates, B.1 (D614G) and Kappa variant, respectively. The animals were monitored daily for the clinical signs and body weight. Throat swab, nasal wash, and organ samples (lungs, nasal turbinate, trachea) were collected and screened using SARS-CoV-2-specific RT-qPCR. Histopathologic evaluation of the lung samples was performed.
Results:
The hamsters infected with the Kappa variant demonstrated increased body weight loss compared to the B.1 lineage isolate. The highest viral RNA load was observed in the nasal turbinate and lung specimens of animals infected with both variants. A significantly higher sgRNA load was observed in the nasal swabs (7 DPI), trachea (3 DPI), and lungs (3 DPI) of hamsters infected with the Kappa variant. Neutralizing antibody response generated in the B.1 lineage-infected hamster sera were comparable against both B.1 and Kappa variant in contrast to Kappa variant-infected hamsters, which showed lower titers against B.1 lineage isolate. Gross and microscopic evaluation of the lung specimens showed severe lung lesions in hamsters infected with Kappa variant compared to B.1.
Conclusions:
The study demonstrates pathogenicity of Kappa variant in hamsters evident with reduced body weight, high viral RNA load in lungs, and pronounced lung lesions. Both Kappa variant- and B.1-infected hamsters produced neutralizing antibodies against both variants studied. |
| doi_str_mv | 10.1089/vbz.2021.0080 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2666549240</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2665166374</sourcerecordid><originalsourceid>FETCH-LOGICAL-c295t-e42c15b0c5eb7d693c3702e09463d8d960d9c0de51763e8f705b7b9cc0f6f9ae3</originalsourceid><addsrcrecordid>eNqF0D1PwzAQBmALgaB8jKzIEgtLytmO7XisKgqIIhAF1shxLpAqTYqdgsqvJ1GBgYXJ59OjV6eXkGMGQwaJOX_PPoccOBsCJLBFBkxKHWktzXY_C4iEUnqP7Icwh44lTO6SPSFl0n34gExmo4dZNG6eI05v7HJp6bP1pa1bOnttPgK9t-1r84J16cp2TcuaWjpb94Be2UVo0dPbJsfqkOwUtgp49P0ekKfJxeP4KpreXV6PR9PIcSPbCGPumMzAScx0roxwQgNHMLESeZIbBblxkKNkWglMCg0y05lxDgpVGIvigJxtcpe-eVthaNNFGRxWla2xWYWUK6VkbHgMHT39Q-fNytfddb2STCmh405FG-V8E4LHIl36cmH9OmWQ9gWnXcFpX3DaF9z5k-_UVbbA_Ff_NNoBsQH92tZ1VWKGvv0n9gsePoUJ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2665166374</pqid></control><display><type>article</type><title>SARS-CoV-2 Kappa Variant Shows Pathogenicity in a Syrian Hamster Model</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Yadav, Pragya D ; Mohandas, Sreelekshmy ; Shete, Anita M ; Nyayanit, Dimpal A ; Gupta, Nivedita ; Patil, Deepak Y ; Sapkal, Gajanan N ; Potdar, Varsha ; Kadam, Manoj ; Kumar, Abhimanyu ; Kumar, Sanjay ; Suryavanshi, Deepak ; Mote, Chandrashekhar S ; Abraham, Priya ; Panda, Samiran ; Bhargava, Balram</creator><creatorcontrib>Yadav, Pragya D ; Mohandas, Sreelekshmy ; Shete, Anita M ; Nyayanit, Dimpal A ; Gupta, Nivedita ; Patil, Deepak Y ; Sapkal, Gajanan N ; Potdar, Varsha ; Kadam, Manoj ; Kumar, Abhimanyu ; Kumar, Sanjay ; Suryavanshi, Deepak ; Mote, Chandrashekhar S ; Abraham, Priya ; Panda, Samiran ; Bhargava, Balram</creatorcontrib><description>Objectives:
The emergence of SARS-CoV-2 lineage B.1.617 variants in India has been associated with a surge in the number of daily infections. We investigated the pathogenic potential of Kappa (B.1.617.1) variant in Syrian golden hamsters.
Methods:
Two groups of Syrian golden hamsters (18 each) were inoculated intranasally with SARS-CoV-2 isolates, B.1 (D614G) and Kappa variant, respectively. The animals were monitored daily for the clinical signs and body weight. Throat swab, nasal wash, and organ samples (lungs, nasal turbinate, trachea) were collected and screened using SARS-CoV-2-specific RT-qPCR. Histopathologic evaluation of the lung samples was performed.
Results:
The hamsters infected with the Kappa variant demonstrated increased body weight loss compared to the B.1 lineage isolate. The highest viral RNA load was observed in the nasal turbinate and lung specimens of animals infected with both variants. A significantly higher sgRNA load was observed in the nasal swabs (7 DPI), trachea (3 DPI), and lungs (3 DPI) of hamsters infected with the Kappa variant. Neutralizing antibody response generated in the B.1 lineage-infected hamster sera were comparable against both B.1 and Kappa variant in contrast to Kappa variant-infected hamsters, which showed lower titers against B.1 lineage isolate. Gross and microscopic evaluation of the lung specimens showed severe lung lesions in hamsters infected with Kappa variant compared to B.1.
Conclusions:
The study demonstrates pathogenicity of Kappa variant in hamsters evident with reduced body weight, high viral RNA load in lungs, and pronounced lung lesions. Both Kappa variant- and B.1-infected hamsters produced neutralizing antibodies against both variants studied.</description><identifier>ISSN: 1530-3667</identifier><identifier>EISSN: 1557-7759</identifier><identifier>DOI: 10.1089/vbz.2021.0080</identifier><identifier>PMID: 35580212</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Animals ; Antibodies ; Antibodies, Neutralizing ; Antibody response ; Body Weight ; Body weight loss ; Bone ; COVID-19 - veterinary ; Cricetinae ; Disease Models, Animal ; Hamsters ; Lesions ; Lungs ; Mesocricetus ; Neutralizing ; Nose ; Original Articles ; Pathogenicity ; Pathogens ; RNA, Viral ; SARS-CoV-2 ; Severe acute respiratory syndrome ; Severe acute respiratory syndrome coronavirus 2 ; Trachea ; Virulence ; Weight loss ; Weight reduction</subject><ispartof>Vector borne and zoonotic diseases (Larchmont, N.Y.), 2022-05, Vol.22 (5), p.289-296</ispartof><rights>2022, Mary Ann Liebert, Inc., publishers</rights><rights>Copyright Mary Ann Liebert, Inc. May 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c295t-e42c15b0c5eb7d693c3702e09463d8d960d9c0de51763e8f705b7b9cc0f6f9ae3</citedby><cites>FETCH-LOGICAL-c295t-e42c15b0c5eb7d693c3702e09463d8d960d9c0de51763e8f705b7b9cc0f6f9ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27928,27929</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35580212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yadav, Pragya D</creatorcontrib><creatorcontrib>Mohandas, Sreelekshmy</creatorcontrib><creatorcontrib>Shete, Anita M</creatorcontrib><creatorcontrib>Nyayanit, Dimpal A</creatorcontrib><creatorcontrib>Gupta, Nivedita</creatorcontrib><creatorcontrib>Patil, Deepak Y</creatorcontrib><creatorcontrib>Sapkal, Gajanan N</creatorcontrib><creatorcontrib>Potdar, Varsha</creatorcontrib><creatorcontrib>Kadam, Manoj</creatorcontrib><creatorcontrib>Kumar, Abhimanyu</creatorcontrib><creatorcontrib>Kumar, Sanjay</creatorcontrib><creatorcontrib>Suryavanshi, Deepak</creatorcontrib><creatorcontrib>Mote, Chandrashekhar S</creatorcontrib><creatorcontrib>Abraham, Priya</creatorcontrib><creatorcontrib>Panda, Samiran</creatorcontrib><creatorcontrib>Bhargava, Balram</creatorcontrib><title>SARS-CoV-2 Kappa Variant Shows Pathogenicity in a Syrian Hamster Model</title><title>Vector borne and zoonotic diseases (Larchmont, N.Y.)</title><addtitle>Vector Borne Zoonotic Dis</addtitle><description>Objectives:
The emergence of SARS-CoV-2 lineage B.1.617 variants in India has been associated with a surge in the number of daily infections. We investigated the pathogenic potential of Kappa (B.1.617.1) variant in Syrian golden hamsters.
Methods:
Two groups of Syrian golden hamsters (18 each) were inoculated intranasally with SARS-CoV-2 isolates, B.1 (D614G) and Kappa variant, respectively. The animals were monitored daily for the clinical signs and body weight. Throat swab, nasal wash, and organ samples (lungs, nasal turbinate, trachea) were collected and screened using SARS-CoV-2-specific RT-qPCR. Histopathologic evaluation of the lung samples was performed.
Results:
The hamsters infected with the Kappa variant demonstrated increased body weight loss compared to the B.1 lineage isolate. The highest viral RNA load was observed in the nasal turbinate and lung specimens of animals infected with both variants. A significantly higher sgRNA load was observed in the nasal swabs (7 DPI), trachea (3 DPI), and lungs (3 DPI) of hamsters infected with the Kappa variant. Neutralizing antibody response generated in the B.1 lineage-infected hamster sera were comparable against both B.1 and Kappa variant in contrast to Kappa variant-infected hamsters, which showed lower titers against B.1 lineage isolate. Gross and microscopic evaluation of the lung specimens showed severe lung lesions in hamsters infected with Kappa variant compared to B.1.
Conclusions:
The study demonstrates pathogenicity of Kappa variant in hamsters evident with reduced body weight, high viral RNA load in lungs, and pronounced lung lesions. Both Kappa variant- and B.1-infected hamsters produced neutralizing antibodies against both variants studied.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Neutralizing</subject><subject>Antibody response</subject><subject>Body Weight</subject><subject>Body weight loss</subject><subject>Bone</subject><subject>COVID-19 - veterinary</subject><subject>Cricetinae</subject><subject>Disease Models, Animal</subject><subject>Hamsters</subject><subject>Lesions</subject><subject>Lungs</subject><subject>Mesocricetus</subject><subject>Neutralizing</subject><subject>Nose</subject><subject>Original Articles</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>RNA, Viral</subject><subject>SARS-CoV-2</subject><subject>Severe acute respiratory syndrome</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Trachea</subject><subject>Virulence</subject><subject>Weight loss</subject><subject>Weight reduction</subject><issn>1530-3667</issn><issn>1557-7759</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0D1PwzAQBmALgaB8jKzIEgtLytmO7XisKgqIIhAF1shxLpAqTYqdgsqvJ1GBgYXJ59OjV6eXkGMGQwaJOX_PPoccOBsCJLBFBkxKHWktzXY_C4iEUnqP7Icwh44lTO6SPSFl0n34gExmo4dZNG6eI05v7HJp6bP1pa1bOnttPgK9t-1r84J16cp2TcuaWjpb94Be2UVo0dPbJsfqkOwUtgp49P0ekKfJxeP4KpreXV6PR9PIcSPbCGPumMzAScx0roxwQgNHMLESeZIbBblxkKNkWglMCg0y05lxDgpVGIvigJxtcpe-eVthaNNFGRxWla2xWYWUK6VkbHgMHT39Q-fNytfddb2STCmh405FG-V8E4LHIl36cmH9OmWQ9gWnXcFpX3DaF9z5k-_UVbbA_Ff_NNoBsQH92tZ1VWKGvv0n9gsePoUJ</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Yadav, Pragya D</creator><creator>Mohandas, Sreelekshmy</creator><creator>Shete, Anita M</creator><creator>Nyayanit, Dimpal A</creator><creator>Gupta, Nivedita</creator><creator>Patil, Deepak Y</creator><creator>Sapkal, Gajanan N</creator><creator>Potdar, Varsha</creator><creator>Kadam, Manoj</creator><creator>Kumar, Abhimanyu</creator><creator>Kumar, Sanjay</creator><creator>Suryavanshi, Deepak</creator><creator>Mote, Chandrashekhar S</creator><creator>Abraham, Priya</creator><creator>Panda, Samiran</creator><creator>Bhargava, Balram</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20220501</creationdate><title>SARS-CoV-2 Kappa Variant Shows Pathogenicity in a Syrian Hamster Model</title><author>Yadav, Pragya D ; Mohandas, Sreelekshmy ; Shete, Anita M ; Nyayanit, Dimpal A ; Gupta, Nivedita ; Patil, Deepak Y ; Sapkal, Gajanan N ; Potdar, Varsha ; Kadam, Manoj ; Kumar, Abhimanyu ; Kumar, Sanjay ; Suryavanshi, Deepak ; Mote, Chandrashekhar S ; Abraham, Priya ; Panda, Samiran ; Bhargava, Balram</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c295t-e42c15b0c5eb7d693c3702e09463d8d960d9c0de51763e8f705b7b9cc0f6f9ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Neutralizing</topic><topic>Antibody response</topic><topic>Body Weight</topic><topic>Body weight loss</topic><topic>Bone</topic><topic>COVID-19 - veterinary</topic><topic>Cricetinae</topic><topic>Disease Models, Animal</topic><topic>Hamsters</topic><topic>Lesions</topic><topic>Lungs</topic><topic>Mesocricetus</topic><topic>Neutralizing</topic><topic>Nose</topic><topic>Original Articles</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>RNA, Viral</topic><topic>SARS-CoV-2</topic><topic>Severe acute respiratory syndrome</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Trachea</topic><topic>Virulence</topic><topic>Weight loss</topic><topic>Weight reduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yadav, Pragya D</creatorcontrib><creatorcontrib>Mohandas, Sreelekshmy</creatorcontrib><creatorcontrib>Shete, Anita M</creatorcontrib><creatorcontrib>Nyayanit, Dimpal A</creatorcontrib><creatorcontrib>Gupta, Nivedita</creatorcontrib><creatorcontrib>Patil, Deepak Y</creatorcontrib><creatorcontrib>Sapkal, Gajanan N</creatorcontrib><creatorcontrib>Potdar, Varsha</creatorcontrib><creatorcontrib>Kadam, Manoj</creatorcontrib><creatorcontrib>Kumar, Abhimanyu</creatorcontrib><creatorcontrib>Kumar, Sanjay</creatorcontrib><creatorcontrib>Suryavanshi, Deepak</creatorcontrib><creatorcontrib>Mote, Chandrashekhar S</creatorcontrib><creatorcontrib>Abraham, Priya</creatorcontrib><creatorcontrib>Panda, Samiran</creatorcontrib><creatorcontrib>Bhargava, Balram</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yadav, Pragya D</au><au>Mohandas, Sreelekshmy</au><au>Shete, Anita M</au><au>Nyayanit, Dimpal A</au><au>Gupta, Nivedita</au><au>Patil, Deepak Y</au><au>Sapkal, Gajanan N</au><au>Potdar, Varsha</au><au>Kadam, Manoj</au><au>Kumar, Abhimanyu</au><au>Kumar, Sanjay</au><au>Suryavanshi, Deepak</au><au>Mote, Chandrashekhar S</au><au>Abraham, Priya</au><au>Panda, Samiran</au><au>Bhargava, Balram</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SARS-CoV-2 Kappa Variant Shows Pathogenicity in a Syrian Hamster Model</atitle><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle><addtitle>Vector Borne Zoonotic Dis</addtitle><date>2022-05-01</date><risdate>2022</risdate><volume>22</volume><issue>5</issue><spage>289</spage><epage>296</epage><pages>289-296</pages><issn>1530-3667</issn><eissn>1557-7759</eissn><abstract>Objectives:
The emergence of SARS-CoV-2 lineage B.1.617 variants in India has been associated with a surge in the number of daily infections. We investigated the pathogenic potential of Kappa (B.1.617.1) variant in Syrian golden hamsters.
Methods:
Two groups of Syrian golden hamsters (18 each) were inoculated intranasally with SARS-CoV-2 isolates, B.1 (D614G) and Kappa variant, respectively. The animals were monitored daily for the clinical signs and body weight. Throat swab, nasal wash, and organ samples (lungs, nasal turbinate, trachea) were collected and screened using SARS-CoV-2-specific RT-qPCR. Histopathologic evaluation of the lung samples was performed.
Results:
The hamsters infected with the Kappa variant demonstrated increased body weight loss compared to the B.1 lineage isolate. The highest viral RNA load was observed in the nasal turbinate and lung specimens of animals infected with both variants. A significantly higher sgRNA load was observed in the nasal swabs (7 DPI), trachea (3 DPI), and lungs (3 DPI) of hamsters infected with the Kappa variant. Neutralizing antibody response generated in the B.1 lineage-infected hamster sera were comparable against both B.1 and Kappa variant in contrast to Kappa variant-infected hamsters, which showed lower titers against B.1 lineage isolate. Gross and microscopic evaluation of the lung specimens showed severe lung lesions in hamsters infected with Kappa variant compared to B.1.
Conclusions:
The study demonstrates pathogenicity of Kappa variant in hamsters evident with reduced body weight, high viral RNA load in lungs, and pronounced lung lesions. Both Kappa variant- and B.1-infected hamsters produced neutralizing antibodies against both variants studied.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>35580212</pmid><doi>10.1089/vbz.2021.0080</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antibodies Antibodies, Neutralizing Antibody response Body Weight Body weight loss Bone COVID-19 - veterinary Cricetinae Disease Models, Animal Hamsters Lesions Lungs Mesocricetus Neutralizing Nose Original Articles Pathogenicity Pathogens RNA, Viral SARS-CoV-2 Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Trachea Virulence Weight loss Weight reduction |
| title | SARS-CoV-2 Kappa Variant Shows Pathogenicity in a Syrian Hamster Model |
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