In vitro biocompatibility testing of 3D printing and conventional resins for occlusal devices
•3D printing resins have shown similar biological effects to conventional ones.•Freeprint Splint was the most cytotoxic on human gingival fibroblasts of the four dental resin studied.•The results obtained in this study suggest that hGFs showed better in vitro biocompatibility after exposition to Key...
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| Veröffentlicht in: | Journal of dentistry 2022-08, Vol.123, p.104163-104163, Article 104163 |
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| creator | Guerrero-Gironés, Julia López-García, Sergio Pecci-Lloret, Miguel R. Pecci-Lloret, María P. Lozano, Francisco Javier Rodríguez García-Bernal, David |
| description | •3D printing resins have shown similar biological effects to conventional ones.•Freeprint Splint was the most cytotoxic on human gingival fibroblasts of the four dental resin studied.•The results obtained in this study suggest that hGFs showed better in vitro biocompatibility after exposition to Keysplint Soft, being the most eligible dental resin for occlusal devices.
: To assess and compare the in vitro biocompatibility of new resins (Keysplint Soft (Keystone Industries), NextDent Ortho Rigid (3D System), and Freeprint Splint (Detax)) and traditional resins (Orthocryl (Dentaurum)) for dental splint.
: Standardized discs (n=40) and 1:1, 1:2, and 1:4 extracts of the tested materials were prepared. Human gingival fibroblasts (hGFs) were isolated from gingival tissues. Different biological tests were carried out, including MTT assays to assess cell metabolic activity, cell migration assays, cell cytoskeleton staining, cell apoptosis, generation of intracellular reactive oxygen species (ROS), and scanning electron microscopy (SEM). Statistical analyses were performed using one-way ANOVA and Tukey's post hoc test (p |
| doi_str_mv | 10.1016/j.jdent.2022.104163 |
| format | Article |
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: To assess and compare the in vitro biocompatibility of new resins (Keysplint Soft (Keystone Industries), NextDent Ortho Rigid (3D System), and Freeprint Splint (Detax)) and traditional resins (Orthocryl (Dentaurum)) for dental splint.
: Standardized discs (n=40) and 1:1, 1:2, and 1:4 extracts of the tested materials were prepared. Human gingival fibroblasts (hGFs) were isolated from gingival tissues. Different biological tests were carried out, including MTT assays to assess cell metabolic activity, cell migration assays, cell cytoskeleton staining, cell apoptosis, generation of intracellular reactive oxygen species (ROS), and scanning electron microscopy (SEM). Statistical analyses were performed using one-way ANOVA and Tukey's post hoc test (p<0.05).
: MTT experiments showed that Freeprint Splint significantly reduces the hGF metabolic activity (***p<0.001), whereas SEM analysis showed almost no cells adhered on its surface. Cell migration was significantly lower after exposure to undiluted extracts of Freeprint Splint at 48 and 72h (***p<0.001). Cell cytoskeleton staining assays showed fewer attached cells in 1:1 and 1:2 dilutions of Freeprint Splint. Annexin-V and 7-AAD staining assays showed that only cells exposed to Keysplint Soft extracts displayed similar cell viability to the control group. Finally, ROS levels detected in undiluted extracts of all resins were significantly enhanced compared to the control group (***p<0.001).
: The 3D-printed resins and the conventional dental resin showed a similar biocompatibility, except for Freeprint Splint, which was the most cytotoxic on hGFs.
: 3D printing has been on the rise in recent years and its use in daily clinical practice is expanding over time. Two of the three 3D-printed resins tested in this study performed as well in the cytotoxicity tests as the conventional one, supporting their use, but caution and further testing are required</description><identifier>ISSN: 0300-5712</identifier><identifier>EISSN: 1879-176X</identifier><identifier>DOI: 10.1016/j.jdent.2022.104163</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>3-D printers ; 3D printing ; Additive manufacturing ; Apoptosis ; Assaying ; Biocompatibility ; bruxism ; Cell migration ; Cell viability ; Cytoskeleton ; Cytotoxicity ; Dental resins ; Dental restorative materials ; Dentistry ; Fibroblasts ; Laboratories ; Manufacturers ; Medical device industry ; Medical equipment ; occlusal devices ; Polymerization ; Reactive oxygen species ; Resins ; Scanning electron microscopy ; Splints ; Staining ; Statistical analysis ; Three dimensional printing ; Toxicity</subject><ispartof>Journal of dentistry, 2022-08, Vol.123, p.104163-104163, Article 104163</ispartof><rights>2022</rights><rights>Copyright Elsevier Limited Aug 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-bd647b77ffd38b954a19c7cb94467d5bff59477c3160c3632c9db56daede13be3</citedby><cites>FETCH-LOGICAL-c339t-bd647b77ffd38b954a19c7cb94467d5bff59477c3160c3632c9db56daede13be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27913,27914</link.rule.ids></links><search><creatorcontrib>Guerrero-Gironés, Julia</creatorcontrib><creatorcontrib>López-García, Sergio</creatorcontrib><creatorcontrib>Pecci-Lloret, Miguel R.</creatorcontrib><creatorcontrib>Pecci-Lloret, María P.</creatorcontrib><creatorcontrib>Lozano, Francisco Javier Rodríguez</creatorcontrib><creatorcontrib>García-Bernal, David</creatorcontrib><title>In vitro biocompatibility testing of 3D printing and conventional resins for occlusal devices</title><title>Journal of dentistry</title><description>•3D printing resins have shown similar biological effects to conventional ones.•Freeprint Splint was the most cytotoxic on human gingival fibroblasts of the four dental resin studied.•The results obtained in this study suggest that hGFs showed better in vitro biocompatibility after exposition to Keysplint Soft, being the most eligible dental resin for occlusal devices.
: To assess and compare the in vitro biocompatibility of new resins (Keysplint Soft (Keystone Industries), NextDent Ortho Rigid (3D System), and Freeprint Splint (Detax)) and traditional resins (Orthocryl (Dentaurum)) for dental splint.
: Standardized discs (n=40) and 1:1, 1:2, and 1:4 extracts of the tested materials were prepared. Human gingival fibroblasts (hGFs) were isolated from gingival tissues. Different biological tests were carried out, including MTT assays to assess cell metabolic activity, cell migration assays, cell cytoskeleton staining, cell apoptosis, generation of intracellular reactive oxygen species (ROS), and scanning electron microscopy (SEM). Statistical analyses were performed using one-way ANOVA and Tukey's post hoc test (p<0.05).
: MTT experiments showed that Freeprint Splint significantly reduces the hGF metabolic activity (***p<0.001), whereas SEM analysis showed almost no cells adhered on its surface. Cell migration was significantly lower after exposure to undiluted extracts of Freeprint Splint at 48 and 72h (***p<0.001). Cell cytoskeleton staining assays showed fewer attached cells in 1:1 and 1:2 dilutions of Freeprint Splint. Annexin-V and 7-AAD staining assays showed that only cells exposed to Keysplint Soft extracts displayed similar cell viability to the control group. Finally, ROS levels detected in undiluted extracts of all resins were significantly enhanced compared to the control group (***p<0.001).
: The 3D-printed resins and the conventional dental resin showed a similar biocompatibility, except for Freeprint Splint, which was the most cytotoxic on hGFs.
: 3D printing has been on the rise in recent years and its use in daily clinical practice is expanding over time. Two of the three 3D-printed resins tested in this study performed as well in the cytotoxicity tests as the conventional one, supporting their use, but caution and further testing are required</description><subject>3-D printers</subject><subject>3D printing</subject><subject>Additive manufacturing</subject><subject>Apoptosis</subject><subject>Assaying</subject><subject>Biocompatibility</subject><subject>bruxism</subject><subject>Cell migration</subject><subject>Cell viability</subject><subject>Cytoskeleton</subject><subject>Cytotoxicity</subject><subject>Dental resins</subject><subject>Dental restorative materials</subject><subject>Dentistry</subject><subject>Fibroblasts</subject><subject>Laboratories</subject><subject>Manufacturers</subject><subject>Medical device industry</subject><subject>Medical equipment</subject><subject>occlusal devices</subject><subject>Polymerization</subject><subject>Reactive oxygen species</subject><subject>Resins</subject><subject>Scanning electron microscopy</subject><subject>Splints</subject><subject>Staining</subject><subject>Statistical analysis</subject><subject>Three dimensional printing</subject><subject>Toxicity</subject><issn>0300-5712</issn><issn>1879-176X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LAzEQxYMoWKufwEvAi5etyWY3aQ4epP4rFLwoeJGwSWYlyzapyW6h397UevLgaZjh92bmPYQuKZlRQvlNN-ss-GFWkrLMk4pydoQmdC5kQQV_P0YTwggpakHLU3SWUkcIqUgpJ-hj6fHWDTFg7YIJ600zOO16N-zwAGlw_hOHFrN7vInO_7SNt9gEv833XPBNjyMk5xNuQ8TBmH5MeWZh6wykc3TSNn2Ci986RW-PD6-L52L18rRc3K0Kw5gcCm15JbQQbWvZXMu6aqg0wmhZVVzYWrdtLSshDKOcGMZZaaTVNbcNWKBMA5ui68PeTQxfY_5brV0y0PeNhzAmVXJe15zyap7Rqz9oF8aYfeypuRRMEMkzxQ6UiSGlCK3K_tdN3ClK1D5y1amfyNU-cnWIPKtuDyrIXrcOokrGgTdgXQQzKBvcv_pvVnKL5A</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>Guerrero-Gironés, Julia</creator><creator>López-García, Sergio</creator><creator>Pecci-Lloret, Miguel R.</creator><creator>Pecci-Lloret, María P.</creator><creator>Lozano, Francisco Javier Rodríguez</creator><creator>García-Bernal, David</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20220801</creationdate><title>In vitro biocompatibility testing of 3D printing and conventional resins for occlusal devices</title><author>Guerrero-Gironés, Julia ; López-García, Sergio ; Pecci-Lloret, Miguel R. ; Pecci-Lloret, María P. ; Lozano, Francisco Javier Rodríguez ; García-Bernal, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-bd647b77ffd38b954a19c7cb94467d5bff59477c3160c3632c9db56daede13be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>3-D printers</topic><topic>3D printing</topic><topic>Additive manufacturing</topic><topic>Apoptosis</topic><topic>Assaying</topic><topic>Biocompatibility</topic><topic>bruxism</topic><topic>Cell migration</topic><topic>Cell viability</topic><topic>Cytoskeleton</topic><topic>Cytotoxicity</topic><topic>Dental resins</topic><topic>Dental restorative materials</topic><topic>Dentistry</topic><topic>Fibroblasts</topic><topic>Laboratories</topic><topic>Manufacturers</topic><topic>Medical device industry</topic><topic>Medical equipment</topic><topic>occlusal devices</topic><topic>Polymerization</topic><topic>Reactive oxygen species</topic><topic>Resins</topic><topic>Scanning electron microscopy</topic><topic>Splints</topic><topic>Staining</topic><topic>Statistical analysis</topic><topic>Three dimensional printing</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guerrero-Gironés, Julia</creatorcontrib><creatorcontrib>López-García, Sergio</creatorcontrib><creatorcontrib>Pecci-Lloret, Miguel R.</creatorcontrib><creatorcontrib>Pecci-Lloret, María P.</creatorcontrib><creatorcontrib>Lozano, Francisco Javier Rodríguez</creatorcontrib><creatorcontrib>García-Bernal, David</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guerrero-Gironés, Julia</au><au>López-García, Sergio</au><au>Pecci-Lloret, Miguel R.</au><au>Pecci-Lloret, María P.</au><au>Lozano, Francisco Javier Rodríguez</au><au>García-Bernal, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro biocompatibility testing of 3D printing and conventional resins for occlusal devices</atitle><jtitle>Journal of dentistry</jtitle><date>2022-08-01</date><risdate>2022</risdate><volume>123</volume><spage>104163</spage><epage>104163</epage><pages>104163-104163</pages><artnum>104163</artnum><issn>0300-5712</issn><eissn>1879-176X</eissn><abstract>•3D printing resins have shown similar biological effects to conventional ones.•Freeprint Splint was the most cytotoxic on human gingival fibroblasts of the four dental resin studied.•The results obtained in this study suggest that hGFs showed better in vitro biocompatibility after exposition to Keysplint Soft, being the most eligible dental resin for occlusal devices.
: To assess and compare the in vitro biocompatibility of new resins (Keysplint Soft (Keystone Industries), NextDent Ortho Rigid (3D System), and Freeprint Splint (Detax)) and traditional resins (Orthocryl (Dentaurum)) for dental splint.
: Standardized discs (n=40) and 1:1, 1:2, and 1:4 extracts of the tested materials were prepared. Human gingival fibroblasts (hGFs) were isolated from gingival tissues. Different biological tests were carried out, including MTT assays to assess cell metabolic activity, cell migration assays, cell cytoskeleton staining, cell apoptosis, generation of intracellular reactive oxygen species (ROS), and scanning electron microscopy (SEM). Statistical analyses were performed using one-way ANOVA and Tukey's post hoc test (p<0.05).
: MTT experiments showed that Freeprint Splint significantly reduces the hGF metabolic activity (***p<0.001), whereas SEM analysis showed almost no cells adhered on its surface. Cell migration was significantly lower after exposure to undiluted extracts of Freeprint Splint at 48 and 72h (***p<0.001). Cell cytoskeleton staining assays showed fewer attached cells in 1:1 and 1:2 dilutions of Freeprint Splint. Annexin-V and 7-AAD staining assays showed that only cells exposed to Keysplint Soft extracts displayed similar cell viability to the control group. Finally, ROS levels detected in undiluted extracts of all resins were significantly enhanced compared to the control group (***p<0.001).
: The 3D-printed resins and the conventional dental resin showed a similar biocompatibility, except for Freeprint Splint, which was the most cytotoxic on hGFs.
: 3D printing has been on the rise in recent years and its use in daily clinical practice is expanding over time. Two of the three 3D-printed resins tested in this study performed as well in the cytotoxicity tests as the conventional one, supporting their use, but caution and further testing are required</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.jdent.2022.104163</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings |
| subjects | 3-D printers 3D printing Additive manufacturing Apoptosis Assaying Biocompatibility bruxism Cell migration Cell viability Cytoskeleton Cytotoxicity Dental resins Dental restorative materials Dentistry Fibroblasts Laboratories Manufacturers Medical device industry Medical equipment occlusal devices Polymerization Reactive oxygen species Resins Scanning electron microscopy Splints Staining Statistical analysis Three dimensional printing Toxicity |
| title | In vitro biocompatibility testing of 3D printing and conventional resins for occlusal devices |
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