Trunk injection delivery of dsRNA for RNAi‐based pest control in apple trees
BACKGROUND RNA interference (RNAi) is a promising new approach for controlling insect pests without the use of synthetic pesticides. Trunk injection is a delivery system for woody plants that harnesses the vascular system of the tree to transport materials to the tree canopy. Full size apple trees w...
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| Veröffentlicht in: | Pest management science 2022-08, Vol.78 (8), p.3528-3533 |
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| container_title | Pest management science |
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| creator | Wise, John C Wise, Annabel G Rakotondravelo, Mamy Vandervoort, Christine Seeve, Candace Fabbri, Brad |
| description | BACKGROUND
RNA interference (RNAi) is a promising new approach for controlling insect pests without the use of synthetic pesticides. Trunk injection is a delivery system for woody plants that harnesses the vascular system of the tree to transport materials to the tree canopy. Full size apple trees were injected with double‐stranded RNA (dsRNA), and season‐long leaf samples were taken to measure the vascular mobility and temporal persistence of dsRNA, using quantitative reverse transcription polymerase chain reaction (qRT‐PCR).
RESULTS
The qRT‐PCR results revealed that the quantities of dsRNA in the apple leaves of treated trees were significantly greater than those in the leaves of untreated trees for both 2019 and 2020 studies. The peak dsRNA concentration in 2019 was 242 pg/30 mg of leaf tissue, and in 2020 was 16.4 pg/30 mg. The persistence of dsRNA in the apple tree canopy in 2019 was at least 84 days, and in 2020 was at least 141 days.
CONCLUSIONS
The highest mean measurement of dsRNA on a single date in 2019 was 242 pg, which is approximately equivalent to 8 ng/1 g leaf tissue. The projection using the highest replicate concentration from the same date is approximately equivalent to 27 ng/1 g leaf tissue, which may be sufficient to be considered biologically active. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. |
| doi_str_mv | 10.1002/ps.6993 |
| format | Article |
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RNA interference (RNAi) is a promising new approach for controlling insect pests without the use of synthetic pesticides. Trunk injection is a delivery system for woody plants that harnesses the vascular system of the tree to transport materials to the tree canopy. Full size apple trees were injected with double‐stranded RNA (dsRNA), and season‐long leaf samples were taken to measure the vascular mobility and temporal persistence of dsRNA, using quantitative reverse transcription polymerase chain reaction (qRT‐PCR).
RESULTS
The qRT‐PCR results revealed that the quantities of dsRNA in the apple leaves of treated trees were significantly greater than those in the leaves of untreated trees for both 2019 and 2020 studies. The peak dsRNA concentration in 2019 was 242 pg/30 mg of leaf tissue, and in 2020 was 16.4 pg/30 mg. The persistence of dsRNA in the apple tree canopy in 2019 was at least 84 days, and in 2020 was at least 141 days.
CONCLUSIONS
The highest mean measurement of dsRNA on a single date in 2019 was 242 pg, which is approximately equivalent to 8 ng/1 g leaf tissue. The projection using the highest replicate concentration from the same date is approximately equivalent to 27 ng/1 g leaf tissue, which may be sufficient to be considered biologically active. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.6993</identifier><identifier>PMID: 35578562</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Apples ; Biological activity ; Canopies ; Chemical pest control ; Double-stranded RNA ; Equivalence ; Fruit trees ; Fruits ; Gene expression ; Harnesses ; Injection ; Insect control ; Insects ; integrated pest management ; Leaves ; Pest control ; Pesticides ; Pests ; Plant tissues ; Polymerase chain reaction ; Reverse transcription ; RNA interference ; RNA-mediated interference ; Trees ; vascular delivery ; Vascular system (plant anatomy) ; Woody plants</subject><ispartof>Pest management science, 2022-08, Vol.78 (8), p.3528-3533</ispartof><rights>2022 The Authors. published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</rights><rights>This article is protected by copyright. All rights reserved.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3783-c95ee01e4f5f77804c74940d8f0fbeb0d4c409a58a73bd7a52fc99e12620e913</citedby><cites>FETCH-LOGICAL-c3783-c95ee01e4f5f77804c74940d8f0fbeb0d4c409a58a73bd7a52fc99e12620e913</cites><orcidid>0000-0002-6138-9481</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.6993$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.6993$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35578562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wise, John C</creatorcontrib><creatorcontrib>Wise, Annabel G</creatorcontrib><creatorcontrib>Rakotondravelo, Mamy</creatorcontrib><creatorcontrib>Vandervoort, Christine</creatorcontrib><creatorcontrib>Seeve, Candace</creatorcontrib><creatorcontrib>Fabbri, Brad</creatorcontrib><title>Trunk injection delivery of dsRNA for RNAi‐based pest control in apple trees</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
RNA interference (RNAi) is a promising new approach for controlling insect pests without the use of synthetic pesticides. Trunk injection is a delivery system for woody plants that harnesses the vascular system of the tree to transport materials to the tree canopy. Full size apple trees were injected with double‐stranded RNA (dsRNA), and season‐long leaf samples were taken to measure the vascular mobility and temporal persistence of dsRNA, using quantitative reverse transcription polymerase chain reaction (qRT‐PCR).
RESULTS
The qRT‐PCR results revealed that the quantities of dsRNA in the apple leaves of treated trees were significantly greater than those in the leaves of untreated trees for both 2019 and 2020 studies. The peak dsRNA concentration in 2019 was 242 pg/30 mg of leaf tissue, and in 2020 was 16.4 pg/30 mg. The persistence of dsRNA in the apple tree canopy in 2019 was at least 84 days, and in 2020 was at least 141 days.
CONCLUSIONS
The highest mean measurement of dsRNA on a single date in 2019 was 242 pg, which is approximately equivalent to 8 ng/1 g leaf tissue. The projection using the highest replicate concentration from the same date is approximately equivalent to 27 ng/1 g leaf tissue, which may be sufficient to be considered biologically active. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</description><subject>Apples</subject><subject>Biological activity</subject><subject>Canopies</subject><subject>Chemical pest control</subject><subject>Double-stranded RNA</subject><subject>Equivalence</subject><subject>Fruit trees</subject><subject>Fruits</subject><subject>Gene expression</subject><subject>Harnesses</subject><subject>Injection</subject><subject>Insect control</subject><subject>Insects</subject><subject>integrated pest management</subject><subject>Leaves</subject><subject>Pest control</subject><subject>Pesticides</subject><subject>Pests</subject><subject>Plant tissues</subject><subject>Polymerase chain reaction</subject><subject>Reverse transcription</subject><subject>RNA interference</subject><subject>RNA-mediated interference</subject><subject>Trees</subject><subject>vascular delivery</subject><subject>Vascular system (plant anatomy)</subject><subject>Woody plants</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNp10N9KwzAUBvAgiptTfAMJeKEgnWnTNMnlGP6DMUV34V1p0xPo7JqatMrufASf0Scxc3MXglfnXPzOx-FD6Dgkw5CQ6LJxw0RKuoP6IYuSIJZS7G538dxDB87NCSFSymgf9ShjXLAk6qPpzHb1Cy7rOai2NDUuoCrfwC6x0bhwj9MR1sZiP8uvj888c1DgBlyLlalbayp_ibOmqQC3FsAdoj2dVQ6ONnOAZtdXs_FtMLm_uRuPJoGiXNBASQZAQog105wLEisey5gUQhOdQ06KWMVEZkxknOYFz1iklZQQRklEQIZ0gM7XsY01r51_J12UTkFVZTWYzqVRkjCWEMqkp6d_6Nx0tvbPeSWEb4FT7tXZWilrnLOg08aWi8wu05Ckq4bTxqWrhr082eR1-QKKrfut1IOLNXgvK1j-l5M-PP3EfQOv1INN</recordid><startdate>202208</startdate><enddate>202208</enddate><creator>Wise, John C</creator><creator>Wise, Annabel G</creator><creator>Rakotondravelo, Mamy</creator><creator>Vandervoort, Christine</creator><creator>Seeve, Candace</creator><creator>Fabbri, Brad</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6138-9481</orcidid></search><sort><creationdate>202208</creationdate><title>Trunk injection delivery of dsRNA for RNAi‐based pest control in apple trees</title><author>Wise, John C ; Wise, Annabel G ; Rakotondravelo, Mamy ; Vandervoort, Christine ; Seeve, Candace ; Fabbri, Brad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3783-c95ee01e4f5f77804c74940d8f0fbeb0d4c409a58a73bd7a52fc99e12620e913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Apples</topic><topic>Biological activity</topic><topic>Canopies</topic><topic>Chemical pest control</topic><topic>Double-stranded RNA</topic><topic>Equivalence</topic><topic>Fruit trees</topic><topic>Fruits</topic><topic>Gene expression</topic><topic>Harnesses</topic><topic>Injection</topic><topic>Insect control</topic><topic>Insects</topic><topic>integrated pest management</topic><topic>Leaves</topic><topic>Pest control</topic><topic>Pesticides</topic><topic>Pests</topic><topic>Plant tissues</topic><topic>Polymerase chain reaction</topic><topic>Reverse transcription</topic><topic>RNA interference</topic><topic>RNA-mediated interference</topic><topic>Trees</topic><topic>vascular delivery</topic><topic>Vascular system (plant anatomy)</topic><topic>Woody plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wise, John C</creatorcontrib><creatorcontrib>Wise, Annabel G</creatorcontrib><creatorcontrib>Rakotondravelo, Mamy</creatorcontrib><creatorcontrib>Vandervoort, Christine</creatorcontrib><creatorcontrib>Seeve, Candace</creatorcontrib><creatorcontrib>Fabbri, Brad</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wise, John C</au><au>Wise, Annabel G</au><au>Rakotondravelo, Mamy</au><au>Vandervoort, Christine</au><au>Seeve, Candace</au><au>Fabbri, Brad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trunk injection delivery of dsRNA for RNAi‐based pest control in apple trees</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2022-08</date><risdate>2022</risdate><volume>78</volume><issue>8</issue><spage>3528</spage><epage>3533</epage><pages>3528-3533</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
RNA interference (RNAi) is a promising new approach for controlling insect pests without the use of synthetic pesticides. Trunk injection is a delivery system for woody plants that harnesses the vascular system of the tree to transport materials to the tree canopy. Full size apple trees were injected with double‐stranded RNA (dsRNA), and season‐long leaf samples were taken to measure the vascular mobility and temporal persistence of dsRNA, using quantitative reverse transcription polymerase chain reaction (qRT‐PCR).
RESULTS
The qRT‐PCR results revealed that the quantities of dsRNA in the apple leaves of treated trees were significantly greater than those in the leaves of untreated trees for both 2019 and 2020 studies. The peak dsRNA concentration in 2019 was 242 pg/30 mg of leaf tissue, and in 2020 was 16.4 pg/30 mg. The persistence of dsRNA in the apple tree canopy in 2019 was at least 84 days, and in 2020 was at least 141 days.
CONCLUSIONS
The highest mean measurement of dsRNA on a single date in 2019 was 242 pg, which is approximately equivalent to 8 ng/1 g leaf tissue. The projection using the highest replicate concentration from the same date is approximately equivalent to 27 ng/1 g leaf tissue, which may be sufficient to be considered biologically active. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>35578562</pmid><doi>10.1002/ps.6993</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-6138-9481</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Apples Biological activity Canopies Chemical pest control Double-stranded RNA Equivalence Fruit trees Fruits Gene expression Harnesses Injection Insect control Insects integrated pest management Leaves Pest control Pesticides Pests Plant tissues Polymerase chain reaction Reverse transcription RNA interference RNA-mediated interference Trees vascular delivery Vascular system (plant anatomy) Woody plants |
| title | Trunk injection delivery of dsRNA for RNAi‐based pest control in apple trees |
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