Evaluation of p21 expression and related autism‐like behavior in Bisphenol‐A exposed offspring of Wistar albino rats
Background Bisphenol A (BPA), an endocrine disruptor, may be involved in the etiology of autism spectrum disorders (ASD); however, the mechanism of neuronal and astrocytic damage remains ambiguous. A possible role of altered expression of p21 in autistic‐like behavior in rat offspring was examined w...
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| Veröffentlicht in: | Birth defects research 2022-07, Vol.114 (11), p.536-550 |
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| creator | Singha, Syna Pervaiz Memon, Samreen Bano, Umbreen Isaac, Amir Derick Shahani, Muhammad Yaqoob |
| description | Background
Bisphenol A (BPA), an endocrine disruptor, may be involved in the etiology of autism spectrum disorders (ASD); however, the mechanism of neuronal and astrocytic damage remains ambiguous. A possible role of altered expression of p21 in autistic‐like behavior in rat offspring was examined with prenatal and postnatal BPA exposure.
Methods
Wistar albino dams were exposed to BPA (5 mg/kg) intraperitoneally throughout pregnancy and until the third postnatal day (PND). Pups were examined on 21st PND for behavioral test. Blood samples were collected for serum lactate levels and pups were sacrificed. Right frontal cortices were dissected out and processed for H&E, immunohistochemical analysis, and gene expression.
Results
Anxiety like behavior and thigmotaxis along with reduction in serum lactate concentrations were observed in pups exposed to BPA. Decline in neuronal number and decreased astrocytic population with reduced dendritic spines were revealed by H&E and immunohistochemical analysis, respectively, in right frontal cortices. Over expression of p21 was also detected in BPA‐exposed offspring.
Conclusions
Over expression of p21 may be associated with autistic behavior. Further studies are recommended to explore the structural alterations in other white matter pathways in frontal cortices. |
| doi_str_mv | 10.1002/bdr2.2022 |
| format | Article |
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Bisphenol A (BPA), an endocrine disruptor, may be involved in the etiology of autism spectrum disorders (ASD); however, the mechanism of neuronal and astrocytic damage remains ambiguous. A possible role of altered expression of p21 in autistic‐like behavior in rat offspring was examined with prenatal and postnatal BPA exposure.
Methods
Wistar albino dams were exposed to BPA (5 mg/kg) intraperitoneally throughout pregnancy and until the third postnatal day (PND). Pups were examined on 21st PND for behavioral test. Blood samples were collected for serum lactate levels and pups were sacrificed. Right frontal cortices were dissected out and processed for H&E, immunohistochemical analysis, and gene expression.
Results
Anxiety like behavior and thigmotaxis along with reduction in serum lactate concentrations were observed in pups exposed to BPA. Decline in neuronal number and decreased astrocytic population with reduced dendritic spines were revealed by H&E and immunohistochemical analysis, respectively, in right frontal cortices. Over expression of p21 was also detected in BPA‐exposed offspring.
Conclusions
Over expression of p21 may be associated with autistic behavior. Further studies are recommended to explore the structural alterations in other white matter pathways in frontal cortices.</description><identifier>ISSN: 2472-1727</identifier><identifier>EISSN: 2472-1727</identifier><identifier>DOI: 10.1002/bdr2.2022</identifier><identifier>PMID: 35560535</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>albino rat ; astrocyte–neuron lactate shuttle ; Autism ; Bisphenol A ; cellular senescence ; cognitive functions ; Cyclin-dependent kinase inhibitor p21 ; Dendritic spines ; Etiology ; Exposure ; Gene expression ; Juveniles ; Lactic acid ; Offspring ; p21‐activated kinases ; Prenatal experience ; Substantia alba ; Thigmotaxis</subject><ispartof>Birth defects research, 2022-07, Vol.114 (11), p.536-550</ispartof><rights>2022 Wiley Periodicals LLC.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2832-ff25902d3e495022a0cf903d25d8d3f04298b6ce72bd39919a25f3a97205ee723</citedby><cites>FETCH-LOGICAL-c2832-ff25902d3e495022a0cf903d25d8d3f04298b6ce72bd39919a25f3a97205ee723</cites><orcidid>0000-0003-4538-2543</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbdr2.2022$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbdr2.2022$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35560535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singha, Syna Pervaiz</creatorcontrib><creatorcontrib>Memon, Samreen</creatorcontrib><creatorcontrib>Bano, Umbreen</creatorcontrib><creatorcontrib>Isaac, Amir Derick</creatorcontrib><creatorcontrib>Shahani, Muhammad Yaqoob</creatorcontrib><title>Evaluation of p21 expression and related autism‐like behavior in Bisphenol‐A exposed offspring of Wistar albino rats</title><title>Birth defects research</title><addtitle>Birth Defects Res</addtitle><description>Background
Bisphenol A (BPA), an endocrine disruptor, may be involved in the etiology of autism spectrum disorders (ASD); however, the mechanism of neuronal and astrocytic damage remains ambiguous. A possible role of altered expression of p21 in autistic‐like behavior in rat offspring was examined with prenatal and postnatal BPA exposure.
Methods
Wistar albino dams were exposed to BPA (5 mg/kg) intraperitoneally throughout pregnancy and until the third postnatal day (PND). Pups were examined on 21st PND for behavioral test. Blood samples were collected for serum lactate levels and pups were sacrificed. Right frontal cortices were dissected out and processed for H&E, immunohistochemical analysis, and gene expression.
Results
Anxiety like behavior and thigmotaxis along with reduction in serum lactate concentrations were observed in pups exposed to BPA. Decline in neuronal number and decreased astrocytic population with reduced dendritic spines were revealed by H&E and immunohistochemical analysis, respectively, in right frontal cortices. Over expression of p21 was also detected in BPA‐exposed offspring.
Conclusions
Over expression of p21 may be associated with autistic behavior. Further studies are recommended to explore the structural alterations in other white matter pathways in frontal cortices.</description><subject>albino rat</subject><subject>astrocyte–neuron lactate shuttle</subject><subject>Autism</subject><subject>Bisphenol A</subject><subject>cellular senescence</subject><subject>cognitive functions</subject><subject>Cyclin-dependent kinase inhibitor p21</subject><subject>Dendritic spines</subject><subject>Etiology</subject><subject>Exposure</subject><subject>Gene expression</subject><subject>Juveniles</subject><subject>Lactic acid</subject><subject>Offspring</subject><subject>p21‐activated kinases</subject><subject>Prenatal experience</subject><subject>Substantia alba</subject><subject>Thigmotaxis</subject><issn>2472-1727</issn><issn>2472-1727</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kcFu1DAQhi1ERau2B14AWeICh23tcZzEx7ZsoVIlJATiGDnxmHXxxsFOtttbH6HPyJPgdAtCSJw8Gn_-5JmfkJecnXDG4LQ1EU6AATwjB1BUsOAVVM__qvfJcUo3jDFeA69E_YLsCylLJoU8INvlRvtJjy70NFg6AKe4HSKmNHd0b2hEr0c0VE-jS-uf9w_efUfa4kpvXIjU9fTcpWGFffD58mx-HlLmg7VpiK7_Nnu_ujTqSLVvXR9o1GM6IntW-4THT-ch-XK5_HzxYXH98f3Vxdn1ooNawMJakIqBEVgomYfUrLOKCQPS1EZYVoCq27LDClojlOJKg7RCqwqYxNwVh-TNzjvE8GPCNDZrlzr0XvcYptRAWRaVKgUvMvr6H_QmTLHPv8tUDaXiTMzU2x3VxZBSRNvkKdc63jWcNXMizZxIMyeS2VdPxqldo_lD_t5_Bk53wK3zePd_U3P-7hM8Kn8BeEWV9w</recordid><startdate>20220701</startdate><enddate>20220701</enddate><creator>Singha, Syna Pervaiz</creator><creator>Memon, Samreen</creator><creator>Bano, Umbreen</creator><creator>Isaac, Amir Derick</creator><creator>Shahani, Muhammad Yaqoob</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4538-2543</orcidid></search><sort><creationdate>20220701</creationdate><title>Evaluation of p21 expression and related autism‐like behavior in Bisphenol‐A exposed offspring of Wistar albino rats</title><author>Singha, Syna Pervaiz ; Memon, Samreen ; Bano, Umbreen ; Isaac, Amir Derick ; Shahani, Muhammad Yaqoob</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2832-ff25902d3e495022a0cf903d25d8d3f04298b6ce72bd39919a25f3a97205ee723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>albino rat</topic><topic>astrocyte–neuron lactate shuttle</topic><topic>Autism</topic><topic>Bisphenol A</topic><topic>cellular senescence</topic><topic>cognitive functions</topic><topic>Cyclin-dependent kinase inhibitor p21</topic><topic>Dendritic spines</topic><topic>Etiology</topic><topic>Exposure</topic><topic>Gene expression</topic><topic>Juveniles</topic><topic>Lactic acid</topic><topic>Offspring</topic><topic>p21‐activated kinases</topic><topic>Prenatal experience</topic><topic>Substantia alba</topic><topic>Thigmotaxis</topic><toplevel>online_resources</toplevel><creatorcontrib>Singha, Syna Pervaiz</creatorcontrib><creatorcontrib>Memon, Samreen</creatorcontrib><creatorcontrib>Bano, Umbreen</creatorcontrib><creatorcontrib>Isaac, Amir Derick</creatorcontrib><creatorcontrib>Shahani, Muhammad Yaqoob</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Birth defects research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singha, Syna Pervaiz</au><au>Memon, Samreen</au><au>Bano, Umbreen</au><au>Isaac, Amir Derick</au><au>Shahani, Muhammad Yaqoob</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of p21 expression and related autism‐like behavior in Bisphenol‐A exposed offspring of Wistar albino rats</atitle><jtitle>Birth defects research</jtitle><addtitle>Birth Defects Res</addtitle><date>2022-07-01</date><risdate>2022</risdate><volume>114</volume><issue>11</issue><spage>536</spage><epage>550</epage><pages>536-550</pages><issn>2472-1727</issn><eissn>2472-1727</eissn><abstract>Background
Bisphenol A (BPA), an endocrine disruptor, may be involved in the etiology of autism spectrum disorders (ASD); however, the mechanism of neuronal and astrocytic damage remains ambiguous. A possible role of altered expression of p21 in autistic‐like behavior in rat offspring was examined with prenatal and postnatal BPA exposure.
Methods
Wistar albino dams were exposed to BPA (5 mg/kg) intraperitoneally throughout pregnancy and until the third postnatal day (PND). Pups were examined on 21st PND for behavioral test. Blood samples were collected for serum lactate levels and pups were sacrificed. Right frontal cortices were dissected out and processed for H&E, immunohistochemical analysis, and gene expression.
Results
Anxiety like behavior and thigmotaxis along with reduction in serum lactate concentrations were observed in pups exposed to BPA. Decline in neuronal number and decreased astrocytic population with reduced dendritic spines were revealed by H&E and immunohistochemical analysis, respectively, in right frontal cortices. Over expression of p21 was also detected in BPA‐exposed offspring.
Conclusions
Over expression of p21 may be associated with autistic behavior. Further studies are recommended to explore the structural alterations in other white matter pathways in frontal cortices.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>35560535</pmid><doi>10.1002/bdr2.2022</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-4538-2543</orcidid></addata></record> |
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| ispartof | Birth defects research, 2022-07, Vol.114 (11), p.536-550 |
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| subjects | albino rat astrocyte–neuron lactate shuttle Autism Bisphenol A cellular senescence cognitive functions Cyclin-dependent kinase inhibitor p21 Dendritic spines Etiology Exposure Gene expression Juveniles Lactic acid Offspring p21‐activated kinases Prenatal experience Substantia alba Thigmotaxis |
| title | Evaluation of p21 expression and related autism‐like behavior in Bisphenol‐A exposed offspring of Wistar albino rats |
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