Development of a novel flow cytometry method for detecting pneumococcal‐specific B cells

Antigen‐specific B cell identification by flow cytometry is crucial for investigating their immunophenotype, subset distribution, and kinetics post‐infection or immunization. Methods using biotinylated polysaccharide antigens have been described, but there is still room for improvement regarding sen...

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Veröffentlicht in:	Cytometry. Part A 2022-07, Vol.101 (7), p.588-596
Hauptverfasser:	Tzovara, Irene, Papadatou, Ioanna, Tzanoudaki, Marianna, Spoulou, Vasiliki
Format:	Artikel
Sprache:	eng
Schlagworte:	13‐valent pneumococcal vaccine Antibodies Antigens antigen‐specific antigen‐specific B cells antigen‐specific multimer Background noise Beads Biotin Epitopes Flow cytometry Fluorescence Immunization Lymphocytes B memory B cells pneumococcal vaccines pneumococcal‐specific B cells Polysaccharides Saccharides Serotypes Titration Vaccine development Vaccines
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