Early effects of adipose-derived stem cell sheets against detrusor underactivity in a rat cryo-injury model
This study investigated the effects of adipose-derived stem cell sheets on a rat model of detrusor underactivity. Adipose-derived stem cell sheets were prepared from the subcutaneous adipose tissue of male Lewis rats. Female Lewis rats were assigned into four groups: control, sham operation, cryo-in...
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| Veröffentlicht in: | Life sciences (1973) 2022-07, Vol.301, p.120604-120604, Article 120604 |
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| container_title | Life sciences (1973) |
| container_volume | 301 |
| creator | Teraoka, Shogo Honda, Masashi Makishima, Karen Shimizu, Ryutaro Tsounapi, Panagiota Yumioka, Tetsuya Iwamoto, Hideto Li, Peili Morizane, Shuichi Hikita, Katsuya Hisatome, Ichiro Takenaka, Atsushi |
| description | This study investigated the effects of adipose-derived stem cell sheets on a rat model of detrusor underactivity.
Adipose-derived stem cell sheets were prepared from the subcutaneous adipose tissue of male Lewis rats. Female Lewis rats were assigned into four groups: control, sham operation, cryo-injury, and cryo-injury+sheet (n = 8 per group). Rats in the cryo-injury+sheet group were implanted with ASC sheets 3 days after cryo-injury induction and underwent cystometry 7 days later. Subsequently, reverse transcription-polymerase chain reaction (RT-PCR) and histopathological examinations were performed. Cell sheets expressing the green fluorescent protein were prepared and transplanted to confirm the viability and differentiation of the sheets. Fluorescence was confirmed using a fluorescence stereomicroscope on days 3, 7, 14, 21, and 28 after sheet implantation, and tissue immunostaining was performed.
Cystometry showed that sheet implantation improved the maximum intravesical pressure (P = 0.009) and the residual urine volume (P = 0.011). Furthermore, RT-PCR indicated that the mRNA levels of the angiogenic factors vascular endothelial growth factor and hepatocyte growth factor were significantly higher in the cryo-injury+sheet group than in the cryo-injury group (P = 0.045, P = 0.037, respectively). Histologically, sheet implantation resulted in an improvement in inflammation and increased the number of blood vessels. Green fluorescent protein-positive cells fused with von Willebrand factor-positive cells and differentiated into blood vessels 7 days after sheet implantation.
Adipose-derived stem cell sheets transplanted into the bladder of cryo-injured rats differentiated into blood vessels and restored bladder contractile function 7 days after transplantation. |
| doi_str_mv | 10.1016/j.lfs.2022.120604 |
| format | Article |
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Adipose-derived stem cell sheets were prepared from the subcutaneous adipose tissue of male Lewis rats. Female Lewis rats were assigned into four groups: control, sham operation, cryo-injury, and cryo-injury+sheet (n = 8 per group). Rats in the cryo-injury+sheet group were implanted with ASC sheets 3 days after cryo-injury induction and underwent cystometry 7 days later. Subsequently, reverse transcription-polymerase chain reaction (RT-PCR) and histopathological examinations were performed. Cell sheets expressing the green fluorescent protein were prepared and transplanted to confirm the viability and differentiation of the sheets. Fluorescence was confirmed using a fluorescence stereomicroscope on days 3, 7, 14, 21, and 28 after sheet implantation, and tissue immunostaining was performed.
Cystometry showed that sheet implantation improved the maximum intravesical pressure (P = 0.009) and the residual urine volume (P = 0.011). Furthermore, RT-PCR indicated that the mRNA levels of the angiogenic factors vascular endothelial growth factor and hepatocyte growth factor were significantly higher in the cryo-injury+sheet group than in the cryo-injury group (P = 0.045, P = 0.037, respectively). Histologically, sheet implantation resulted in an improvement in inflammation and increased the number of blood vessels. Green fluorescent protein-positive cells fused with von Willebrand factor-positive cells and differentiated into blood vessels 7 days after sheet implantation.
Adipose-derived stem cell sheets transplanted into the bladder of cryo-injured rats differentiated into blood vessels and restored bladder contractile function 7 days after transplantation.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2022.120604</identifier><identifier>PMID: 35526593</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adipose tissue ; Adipose-derived stem cells ; Angiogenesis ; Bladder ; Blood vessels ; Cell differentiation ; Cell sheets ; Contractility ; Cystometry ; Detrusor underactivity ; Fluorescence ; Green fluorescent protein ; Growth factors ; Hepatocyte growth factor ; Implantation ; Injuries ; Polymerase chain reaction ; Proteins ; Regenerative medicine ; Reverse transcription ; Stem cells ; Tissue engineering ; Transplantation ; Vascular endothelial growth factor ; Von Willebrand factor</subject><ispartof>Life sciences (1973), 2022-07, Vol.301, p.120604-120604, Article 120604</ispartof><rights>2022 The Authors</rights><rights>Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier BV Jul 15, 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-367aad410238c886b7a52029bb4ffe728e6e2b8e6810039efd86c1402c3974113</citedby><cites>FETCH-LOGICAL-c490t-367aad410238c886b7a52029bb4ffe728e6e2b8e6810039efd86c1402c3974113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2022.120604$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35526593$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teraoka, Shogo</creatorcontrib><creatorcontrib>Honda, Masashi</creatorcontrib><creatorcontrib>Makishima, Karen</creatorcontrib><creatorcontrib>Shimizu, Ryutaro</creatorcontrib><creatorcontrib>Tsounapi, Panagiota</creatorcontrib><creatorcontrib>Yumioka, Tetsuya</creatorcontrib><creatorcontrib>Iwamoto, Hideto</creatorcontrib><creatorcontrib>Li, Peili</creatorcontrib><creatorcontrib>Morizane, Shuichi</creatorcontrib><creatorcontrib>Hikita, Katsuya</creatorcontrib><creatorcontrib>Hisatome, Ichiro</creatorcontrib><creatorcontrib>Takenaka, Atsushi</creatorcontrib><title>Early effects of adipose-derived stem cell sheets against detrusor underactivity in a rat cryo-injury model</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>This study investigated the effects of adipose-derived stem cell sheets on a rat model of detrusor underactivity.
Adipose-derived stem cell sheets were prepared from the subcutaneous adipose tissue of male Lewis rats. Female Lewis rats were assigned into four groups: control, sham operation, cryo-injury, and cryo-injury+sheet (n = 8 per group). Rats in the cryo-injury+sheet group were implanted with ASC sheets 3 days after cryo-injury induction and underwent cystometry 7 days later. Subsequently, reverse transcription-polymerase chain reaction (RT-PCR) and histopathological examinations were performed. Cell sheets expressing the green fluorescent protein were prepared and transplanted to confirm the viability and differentiation of the sheets. Fluorescence was confirmed using a fluorescence stereomicroscope on days 3, 7, 14, 21, and 28 after sheet implantation, and tissue immunostaining was performed.
Cystometry showed that sheet implantation improved the maximum intravesical pressure (P = 0.009) and the residual urine volume (P = 0.011). Furthermore, RT-PCR indicated that the mRNA levels of the angiogenic factors vascular endothelial growth factor and hepatocyte growth factor were significantly higher in the cryo-injury+sheet group than in the cryo-injury group (P = 0.045, P = 0.037, respectively). Histologically, sheet implantation resulted in an improvement in inflammation and increased the number of blood vessels. Green fluorescent protein-positive cells fused with von Willebrand factor-positive cells and differentiated into blood vessels 7 days after sheet implantation.
Adipose-derived stem cell sheets transplanted into the bladder of cryo-injured rats differentiated into blood vessels and restored bladder contractile function 7 days after transplantation.</description><subject>Adipose tissue</subject><subject>Adipose-derived stem cells</subject><subject>Angiogenesis</subject><subject>Bladder</subject><subject>Blood vessels</subject><subject>Cell differentiation</subject><subject>Cell sheets</subject><subject>Contractility</subject><subject>Cystometry</subject><subject>Detrusor underactivity</subject><subject>Fluorescence</subject><subject>Green fluorescent protein</subject><subject>Growth factors</subject><subject>Hepatocyte growth factor</subject><subject>Implantation</subject><subject>Injuries</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Regenerative medicine</subject><subject>Reverse transcription</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Transplantation</subject><subject>Vascular endothelial growth factor</subject><subject>Von Willebrand factor</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAQhq0K1G4LD8AFWeLCJcvYThxHnFDVFqRKXOBsOfYEHJJ4sZ2V8vZ4tS0HDlxmLt_8mpmPkDcM9gyY_DDupyHtOXC-Zxwk1Bdkx1TbVSAFe0F2ALyuBIfmilynNAJA07TiklyJpuGy6cSO_LozcdooDgPanGgYqHH-EBJWDqM_oqMp40wtThNNPxELY34Yv6RMHea4phDpuhTW2OyPPm_UL9TQaDK1cQuVX8Y1bnQODqdX5OVgpoSvn_oN-X5_9-32c_X49eHL7afHytYd5ErI1hhXM-BCWaVk35qm3Nj1fV22bLlCibwvVTEA0eHglLSsBm5F19aMiRvy_px7iOH3iinr2afTBWbBsCbNpWS1Yp0UBX33DzqGNS5lu0K1qmGiVlAodqZsDClFHPQh-tnETTPQJxN61MWEPpnQZxNl5u1T8trP6P5OPL--AB_PAJZXHD1GnazHxaLzsbjQLvj_xP8BEzGYhg</recordid><startdate>20220715</startdate><enddate>20220715</enddate><creator>Teraoka, Shogo</creator><creator>Honda, Masashi</creator><creator>Makishima, Karen</creator><creator>Shimizu, Ryutaro</creator><creator>Tsounapi, Panagiota</creator><creator>Yumioka, Tetsuya</creator><creator>Iwamoto, Hideto</creator><creator>Li, Peili</creator><creator>Morizane, Shuichi</creator><creator>Hikita, Katsuya</creator><creator>Hisatome, Ichiro</creator><creator>Takenaka, Atsushi</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20220715</creationdate><title>Early effects of adipose-derived stem cell sheets against detrusor underactivity in a rat cryo-injury model</title><author>Teraoka, Shogo ; Honda, Masashi ; Makishima, Karen ; Shimizu, Ryutaro ; Tsounapi, Panagiota ; Yumioka, Tetsuya ; Iwamoto, Hideto ; Li, Peili ; Morizane, Shuichi ; Hikita, Katsuya ; Hisatome, Ichiro ; Takenaka, Atsushi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-367aad410238c886b7a52029bb4ffe728e6e2b8e6810039efd86c1402c3974113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adipose tissue</topic><topic>Adipose-derived stem cells</topic><topic>Angiogenesis</topic><topic>Bladder</topic><topic>Blood vessels</topic><topic>Cell differentiation</topic><topic>Cell sheets</topic><topic>Contractility</topic><topic>Cystometry</topic><topic>Detrusor underactivity</topic><topic>Fluorescence</topic><topic>Green fluorescent protein</topic><topic>Growth factors</topic><topic>Hepatocyte growth factor</topic><topic>Implantation</topic><topic>Injuries</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Regenerative medicine</topic><topic>Reverse transcription</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Transplantation</topic><topic>Vascular endothelial growth factor</topic><topic>Von Willebrand factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teraoka, Shogo</creatorcontrib><creatorcontrib>Honda, Masashi</creatorcontrib><creatorcontrib>Makishima, Karen</creatorcontrib><creatorcontrib>Shimizu, Ryutaro</creatorcontrib><creatorcontrib>Tsounapi, Panagiota</creatorcontrib><creatorcontrib>Yumioka, Tetsuya</creatorcontrib><creatorcontrib>Iwamoto, Hideto</creatorcontrib><creatorcontrib>Li, Peili</creatorcontrib><creatorcontrib>Morizane, Shuichi</creatorcontrib><creatorcontrib>Hikita, Katsuya</creatorcontrib><creatorcontrib>Hisatome, Ichiro</creatorcontrib><creatorcontrib>Takenaka, Atsushi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teraoka, Shogo</au><au>Honda, Masashi</au><au>Makishima, Karen</au><au>Shimizu, Ryutaro</au><au>Tsounapi, Panagiota</au><au>Yumioka, Tetsuya</au><au>Iwamoto, Hideto</au><au>Li, Peili</au><au>Morizane, Shuichi</au><au>Hikita, Katsuya</au><au>Hisatome, Ichiro</au><au>Takenaka, Atsushi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early effects of adipose-derived stem cell sheets against detrusor underactivity in a rat cryo-injury model</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2022-07-15</date><risdate>2022</risdate><volume>301</volume><spage>120604</spage><epage>120604</epage><pages>120604-120604</pages><artnum>120604</artnum><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>This study investigated the effects of adipose-derived stem cell sheets on a rat model of detrusor underactivity.
Adipose-derived stem cell sheets were prepared from the subcutaneous adipose tissue of male Lewis rats. Female Lewis rats were assigned into four groups: control, sham operation, cryo-injury, and cryo-injury+sheet (n = 8 per group). Rats in the cryo-injury+sheet group were implanted with ASC sheets 3 days after cryo-injury induction and underwent cystometry 7 days later. Subsequently, reverse transcription-polymerase chain reaction (RT-PCR) and histopathological examinations were performed. Cell sheets expressing the green fluorescent protein were prepared and transplanted to confirm the viability and differentiation of the sheets. Fluorescence was confirmed using a fluorescence stereomicroscope on days 3, 7, 14, 21, and 28 after sheet implantation, and tissue immunostaining was performed.
Cystometry showed that sheet implantation improved the maximum intravesical pressure (P = 0.009) and the residual urine volume (P = 0.011). Furthermore, RT-PCR indicated that the mRNA levels of the angiogenic factors vascular endothelial growth factor and hepatocyte growth factor were significantly higher in the cryo-injury+sheet group than in the cryo-injury group (P = 0.045, P = 0.037, respectively). Histologically, sheet implantation resulted in an improvement in inflammation and increased the number of blood vessels. Green fluorescent protein-positive cells fused with von Willebrand factor-positive cells and differentiated into blood vessels 7 days after sheet implantation.
Adipose-derived stem cell sheets transplanted into the bladder of cryo-injured rats differentiated into blood vessels and restored bladder contractile function 7 days after transplantation.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>35526593</pmid><doi>10.1016/j.lfs.2022.120604</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose tissue Adipose-derived stem cells Angiogenesis Bladder Blood vessels Cell differentiation Cell sheets Contractility Cystometry Detrusor underactivity Fluorescence Green fluorescent protein Growth factors Hepatocyte growth factor Implantation Injuries Polymerase chain reaction Proteins Regenerative medicine Reverse transcription Stem cells Tissue engineering Transplantation Vascular endothelial growth factor Von Willebrand factor |
| title | Early effects of adipose-derived stem cell sheets against detrusor underactivity in a rat cryo-injury model |
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