Silencing of Rab23 by siRNA inhibits ultraviolet B‐induced melanogenesis via downregulation of PKA/CREB/MITF
Recent investigations have shown that the Rab family of GTPases is associated with all aspects of melanogenesis. However, the effect of Rab23, which localizes to the plasma membrane and regulates the endocytic pathway within eukaryotic cells, in melanogenesis has not been reported. To understand the...
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| Veröffentlicht in: | Experimental dermatology 2022-08, Vol.31 (8), p.1253-1263 |
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| creator | Wei, Xuanjin Huang, Min Yang, Yi Liu, Yali Chi, Sumin Li, Chengxin |
| description | Recent investigations have shown that the Rab family of GTPases is associated with all aspects of melanogenesis. However, the effect of Rab23, which localizes to the plasma membrane and regulates the endocytic pathway within eukaryotic cells, in melanogenesis has not been reported. To understand the role of Rab23 in UVB‐induced melanogenesis, we evaluated changes in the level of melanin, activity of tyrosinase and levels of melanogenesis‐related proteins such as microphthalmia transcription factor and tyrosinase‐related protein‐1 (TRP‐1) and the melanosome transport‐related protein complex Rab27a‐melanophilin‐myosin Va after the downregulation of Rab23 in B16F10 and SK‐MEL‐2 cells with or without UVB irradiation. Our results showed that downregulating Rab23 reduced the melanin level and tyrosinase activity and inhibited the expression of proteins involved in UVB‐induced melanogenesis. Rab23 colocalized with mature melanosomes marked with TRP‐1. Furthermore, downregulating Rab23 induced the abnormal accumulation of melanosomes around the nucleus. We demonstrated that the downregulation of Rab23 inhibited melanin synthesis and melanosome transport by decreasing the PKA/CREB/MITF pathway, which is the key regulator of UVB‐induced melanogenesis. |
| doi_str_mv | 10.1111/exd.14586 |
| format | Article |
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However, the effect of Rab23, which localizes to the plasma membrane and regulates the endocytic pathway within eukaryotic cells, in melanogenesis has not been reported. To understand the role of Rab23 in UVB‐induced melanogenesis, we evaluated changes in the level of melanin, activity of tyrosinase and levels of melanogenesis‐related proteins such as microphthalmia transcription factor and tyrosinase‐related protein‐1 (TRP‐1) and the melanosome transport‐related protein complex Rab27a‐melanophilin‐myosin Va after the downregulation of Rab23 in B16F10 and SK‐MEL‐2 cells with or without UVB irradiation. Our results showed that downregulating Rab23 reduced the melanin level and tyrosinase activity and inhibited the expression of proteins involved in UVB‐induced melanogenesis. Rab23 colocalized with mature melanosomes marked with TRP‐1. Furthermore, downregulating Rab23 induced the abnormal accumulation of melanosomes around the nucleus. We demonstrated that the downregulation of Rab23 inhibited melanin synthesis and melanosome transport by decreasing the PKA/CREB/MITF pathway, which is the key regulator of UVB‐induced melanogenesis.</description><identifier>ISSN: 0906-6705</identifier><identifier>EISSN: 1600-0625</identifier><identifier>DOI: 10.1111/exd.14586</identifier><identifier>PMID: 35514241</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Cyclic AMP response element-binding protein ; Melanin ; melanogenesis ; melanosome transport ; Melanosomes ; Microphthalmia-associated transcription factor ; Myosin ; Protein transport ; Rab23 ; siRNA ; ultraviolet B</subject><ispartof>Experimental dermatology, 2022-08, Vol.31 (8), p.1253-1263</ispartof><rights>2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3136-92aa9fbd9c25d8969e0ec1f1f5f9f6ed6cbe376306641d7137748ae87152523c3</cites><orcidid>0000-0002-3514-5985</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fexd.14586$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fexd.14586$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35514241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, Xuanjin</creatorcontrib><creatorcontrib>Huang, Min</creatorcontrib><creatorcontrib>Yang, Yi</creatorcontrib><creatorcontrib>Liu, Yali</creatorcontrib><creatorcontrib>Chi, Sumin</creatorcontrib><creatorcontrib>Li, Chengxin</creatorcontrib><title>Silencing of Rab23 by siRNA inhibits ultraviolet B‐induced melanogenesis via downregulation of PKA/CREB/MITF</title><title>Experimental dermatology</title><addtitle>Exp Dermatol</addtitle><description>Recent investigations have shown that the Rab family of GTPases is associated with all aspects of melanogenesis. However, the effect of Rab23, which localizes to the plasma membrane and regulates the endocytic pathway within eukaryotic cells, in melanogenesis has not been reported. To understand the role of Rab23 in UVB‐induced melanogenesis, we evaluated changes in the level of melanin, activity of tyrosinase and levels of melanogenesis‐related proteins such as microphthalmia transcription factor and tyrosinase‐related protein‐1 (TRP‐1) and the melanosome transport‐related protein complex Rab27a‐melanophilin‐myosin Va after the downregulation of Rab23 in B16F10 and SK‐MEL‐2 cells with or without UVB irradiation. Our results showed that downregulating Rab23 reduced the melanin level and tyrosinase activity and inhibited the expression of proteins involved in UVB‐induced melanogenesis. Rab23 colocalized with mature melanosomes marked with TRP‐1. Furthermore, downregulating Rab23 induced the abnormal accumulation of melanosomes around the nucleus. We demonstrated that the downregulation of Rab23 inhibited melanin synthesis and melanosome transport by decreasing the PKA/CREB/MITF pathway, which is the key regulator of UVB‐induced melanogenesis.</description><subject>Cyclic AMP response element-binding protein</subject><subject>Melanin</subject><subject>melanogenesis</subject><subject>melanosome transport</subject><subject>Melanosomes</subject><subject>Microphthalmia-associated transcription factor</subject><subject>Myosin</subject><subject>Protein transport</subject><subject>Rab23</subject><subject>siRNA</subject><subject>ultraviolet B</subject><issn>0906-6705</issn><issn>1600-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kc1uEzEUhS1ERdOWBS-ALLGhi2n8M7bHyzSkUFEKCkViZ3nsO8HVxG7HMy3Z8Qg8I0_SCSkskLibu_n06egchF5QckLHm8J3f0JLUcknaEIlIQWRTDxFE6KJLKQiYh8d5HxNCFVciWdonwtBS1bSCYqfQwvRhbjCqcFLWzOO6w3OYXk5wyF-C3XoMx7avrN3IbXQ49NfP36G6AcHHq-htTGtIEIOGd8Fi326jx2shtb2IcWt89P72XS-XJxOP5xfnR2hvca2GZ4__kP05WxxNX9XXHx8ez6fXRSOUy4LzazVTe21Y8JXWmog4GhDG9HoRoKXrgauJCdSltQrypUqKwuVooIJxh0_RK933psu3Q6Qe7MO2UE7xoU0ZMOkpKTiWssRffUPep2GLo7pDFOEKVERtaWOd5TrUs4dNOamC2vbbQwlZjuCGUcwv0cY2ZePxqFeg_9L_ml9BKY74H4sf_N_k1l8fbNTPgCY74_M</recordid><startdate>202208</startdate><enddate>202208</enddate><creator>Wei, Xuanjin</creator><creator>Huang, Min</creator><creator>Yang, Yi</creator><creator>Liu, Yali</creator><creator>Chi, Sumin</creator><creator>Li, Chengxin</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3514-5985</orcidid></search><sort><creationdate>202208</creationdate><title>Silencing of Rab23 by siRNA inhibits ultraviolet B‐induced melanogenesis via downregulation of PKA/CREB/MITF</title><author>Wei, Xuanjin ; Huang, Min ; Yang, Yi ; Liu, Yali ; Chi, Sumin ; Li, Chengxin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3136-92aa9fbd9c25d8969e0ec1f1f5f9f6ed6cbe376306641d7137748ae87152523c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Cyclic AMP response element-binding protein</topic><topic>Melanin</topic><topic>melanogenesis</topic><topic>melanosome transport</topic><topic>Melanosomes</topic><topic>Microphthalmia-associated transcription factor</topic><topic>Myosin</topic><topic>Protein transport</topic><topic>Rab23</topic><topic>siRNA</topic><topic>ultraviolet B</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Xuanjin</creatorcontrib><creatorcontrib>Huang, Min</creatorcontrib><creatorcontrib>Yang, Yi</creatorcontrib><creatorcontrib>Liu, Yali</creatorcontrib><creatorcontrib>Chi, Sumin</creatorcontrib><creatorcontrib>Li, Chengxin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Xuanjin</au><au>Huang, Min</au><au>Yang, Yi</au><au>Liu, Yali</au><au>Chi, Sumin</au><au>Li, Chengxin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silencing of Rab23 by siRNA inhibits ultraviolet B‐induced melanogenesis via downregulation of PKA/CREB/MITF</atitle><jtitle>Experimental dermatology</jtitle><addtitle>Exp Dermatol</addtitle><date>2022-08</date><risdate>2022</risdate><volume>31</volume><issue>8</issue><spage>1253</spage><epage>1263</epage><pages>1253-1263</pages><issn>0906-6705</issn><eissn>1600-0625</eissn><abstract>Recent investigations have shown that the Rab family of GTPases is associated with all aspects of melanogenesis. However, the effect of Rab23, which localizes to the plasma membrane and regulates the endocytic pathway within eukaryotic cells, in melanogenesis has not been reported. To understand the role of Rab23 in UVB‐induced melanogenesis, we evaluated changes in the level of melanin, activity of tyrosinase and levels of melanogenesis‐related proteins such as microphthalmia transcription factor and tyrosinase‐related protein‐1 (TRP‐1) and the melanosome transport‐related protein complex Rab27a‐melanophilin‐myosin Va after the downregulation of Rab23 in B16F10 and SK‐MEL‐2 cells with or without UVB irradiation. Our results showed that downregulating Rab23 reduced the melanin level and tyrosinase activity and inhibited the expression of proteins involved in UVB‐induced melanogenesis. Rab23 colocalized with mature melanosomes marked with TRP‐1. Furthermore, downregulating Rab23 induced the abnormal accumulation of melanosomes around the nucleus. We demonstrated that the downregulation of Rab23 inhibited melanin synthesis and melanosome transport by decreasing the PKA/CREB/MITF pathway, which is the key regulator of UVB‐induced melanogenesis.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35514241</pmid><doi>10.1111/exd.14586</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-3514-5985</orcidid></addata></record> |
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| subjects | Cyclic AMP response element-binding protein Melanin melanogenesis melanosome transport Melanosomes Microphthalmia-associated transcription factor Myosin Protein transport Rab23 siRNA ultraviolet B |
| title | Silencing of Rab23 by siRNA inhibits ultraviolet B‐induced melanogenesis via downregulation of PKA/CREB/MITF |
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