Raman spectroscopy and sciatic functional index (SFI) after low-level laser therapy (LLLT) in a rat sciatic nerve crush injury model
Axonotmesis causes sensorimotor and neurofunctional deficits, and its regeneration can occur slowly or not occur if not treated appropriately. Low-level laser therapy (LLLT) promotes nerve regeneration with the proliferation of myelinating Schwann cells to recover the myelin sheath and the productio...
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| Veröffentlicht in: | Lasers in medical science 2022-09, Vol.37 (7), p.2957-2971 |
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| creator | de Almeida Melo Maciel Mangueira, Melissa Caparelli-Dáquer, Egas Filho, Ozimo Pereira Gama de Assis, Diogo Souza Ferreira Rubim Sousa, Janyeid Karla Castro Lima, Willy Leite Pinheiro, Antonio Luiz Barbosa Silveira, Landulfo Mangueira, Nilton Maciel |
| description | Axonotmesis causes sensorimotor and neurofunctional deficits, and its regeneration can occur slowly or not occur if not treated appropriately. Low-level laser therapy (LLLT) promotes nerve regeneration with the proliferation of myelinating Schwann cells to recover the myelin sheath and the production of glycoproteins for endoneurium reconstruction. This study aimed to evaluate the effects of LLLT on sciatic nerve regeneration after compression injury by means of the sciatic functional index (SFI) and Raman spectroscopy (RS). For this, 64 Wistar rats were divided into two groups according to the length of treatment: 14 days (
n
= 32) and 21 days (
n
= 32). These two groups were subdivided into four sub-groups of eight animals each (control 1; control 2; laser 660 nm; laser 808 nm). All animals had surgical exposure to the sciatic nerve, and only control 1 did not suffer nerve damage. To cause the lesion in the sciatic nerve, compression was applied with a Kelly clamp for 6 s. The evaluation of sensory deficit was performed by the painful exteroceptive sensitivity (PES) and neuromotor tests by the SFI. Laser 660 nm and laser 808 nm sub-groups were irradiated daily (100 mW, 40 s, energy density of 133 J/cm
2
). The sciatic nerve segment was removed for RS analysis. The animals showed accentuated sensory and neurofunctional deficit after injury and their rehabilitation occurred more effectively in the sub-groups treated with 660 nm laser. Control 2 sub-group did not obtain functional recovery of gait. The RS identified sphingolipids (718, 1065, and 1440 cm
−1
) and collagen (700, 852, 1004, 1270, and 1660 cm
−1
) as biomolecular characteristics of sciatic nerves. Principal component analysis revealed important differences among sub-groups and a directly proportional correlation with SFI, mainly in the sub-group laser 660 nm treated for 21 days. In the axonotmesis-type lesion model presented herein, the 660 nm laser was more efficient in neurofunctional recovery, and the Raman spectra of lipid and protein properties were attributed to the basic biochemical composition of the sciatic nerve. |
| doi_str_mv | 10.1007/s10103-022-03565-5 |
| format | Article |
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n
= 32) and 21 days (
n
= 32). These two groups were subdivided into four sub-groups of eight animals each (control 1; control 2; laser 660 nm; laser 808 nm). All animals had surgical exposure to the sciatic nerve, and only control 1 did not suffer nerve damage. To cause the lesion in the sciatic nerve, compression was applied with a Kelly clamp for 6 s. The evaluation of sensory deficit was performed by the painful exteroceptive sensitivity (PES) and neuromotor tests by the SFI. Laser 660 nm and laser 808 nm sub-groups were irradiated daily (100 mW, 40 s, energy density of 133 J/cm
2
). The sciatic nerve segment was removed for RS analysis. The animals showed accentuated sensory and neurofunctional deficit after injury and their rehabilitation occurred more effectively in the sub-groups treated with 660 nm laser. Control 2 sub-group did not obtain functional recovery of gait. The RS identified sphingolipids (718, 1065, and 1440 cm
−1
) and collagen (700, 852, 1004, 1270, and 1660 cm
−1
) as biomolecular characteristics of sciatic nerves. Principal component analysis revealed important differences among sub-groups and a directly proportional correlation with SFI, mainly in the sub-group laser 660 nm treated for 21 days. In the axonotmesis-type lesion model presented herein, the 660 nm laser was more efficient in neurofunctional recovery, and the Raman spectra of lipid and protein properties were attributed to the basic biochemical composition of the sciatic nerve.</description><identifier>ISSN: 1435-604X</identifier><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-022-03565-5</identifier><identifier>PMID: 35503388</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Animal models ; Animals ; Biochemical composition ; Cell proliferation ; Collagen ; Compression ; Dentistry ; Gait ; Glycoproteins ; Injuries ; Injury analysis ; Lasers ; Lesions ; Lipids ; Medicine ; Medicine & Public Health ; Myelin ; Nerves ; Optical Devices ; Optics ; Original Article ; Photonics ; Principal components analysis ; Quantum Optics ; Raman spectra ; Raman spectroscopy ; Recovery ; Recovery of function ; Regeneration ; Rehabilitation ; Schwann cells ; Sciatic nerve ; Sensorimotor system ; Sheaths ; Spectroscopy ; Spectrum analysis ; Sphingolipids</subject><ispartof>Lasers in medical science, 2022-09, Vol.37 (7), p.2957-2971</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-c98c30e4cbb85fcd1ce6d61f108bc8afb439995d403bc0d33393808ad1157d883</citedby><cites>FETCH-LOGICAL-c375t-c98c30e4cbb85fcd1ce6d61f108bc8afb439995d403bc0d33393808ad1157d883</cites><orcidid>0000-0001-9138-9557</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-022-03565-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-022-03565-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35503388$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Almeida Melo Maciel Mangueira, Melissa</creatorcontrib><creatorcontrib>Caparelli-Dáquer, Egas</creatorcontrib><creatorcontrib>Filho, Ozimo Pereira Gama</creatorcontrib><creatorcontrib>de Assis, Diogo Souza Ferreira Rubim</creatorcontrib><creatorcontrib>Sousa, Janyeid Karla Castro</creatorcontrib><creatorcontrib>Lima, Willy Leite</creatorcontrib><creatorcontrib>Pinheiro, Antonio Luiz Barbosa</creatorcontrib><creatorcontrib>Silveira, Landulfo</creatorcontrib><creatorcontrib>Mangueira, Nilton Maciel</creatorcontrib><title>Raman spectroscopy and sciatic functional index (SFI) after low-level laser therapy (LLLT) in a rat sciatic nerve crush injury model</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>Axonotmesis causes sensorimotor and neurofunctional deficits, and its regeneration can occur slowly or not occur if not treated appropriately. Low-level laser therapy (LLLT) promotes nerve regeneration with the proliferation of myelinating Schwann cells to recover the myelin sheath and the production of glycoproteins for endoneurium reconstruction. This study aimed to evaluate the effects of LLLT on sciatic nerve regeneration after compression injury by means of the sciatic functional index (SFI) and Raman spectroscopy (RS). For this, 64 Wistar rats were divided into two groups according to the length of treatment: 14 days (
n
= 32) and 21 days (
n
= 32). These two groups were subdivided into four sub-groups of eight animals each (control 1; control 2; laser 660 nm; laser 808 nm). All animals had surgical exposure to the sciatic nerve, and only control 1 did not suffer nerve damage. To cause the lesion in the sciatic nerve, compression was applied with a Kelly clamp for 6 s. The evaluation of sensory deficit was performed by the painful exteroceptive sensitivity (PES) and neuromotor tests by the SFI. Laser 660 nm and laser 808 nm sub-groups were irradiated daily (100 mW, 40 s, energy density of 133 J/cm
2
). The sciatic nerve segment was removed for RS analysis. The animals showed accentuated sensory and neurofunctional deficit after injury and their rehabilitation occurred more effectively in the sub-groups treated with 660 nm laser. Control 2 sub-group did not obtain functional recovery of gait. The RS identified sphingolipids (718, 1065, and 1440 cm
−1
) and collagen (700, 852, 1004, 1270, and 1660 cm
−1
) as biomolecular characteristics of sciatic nerves. Principal component analysis revealed important differences among sub-groups and a directly proportional correlation with SFI, mainly in the sub-group laser 660 nm treated for 21 days. In the axonotmesis-type lesion model presented herein, the 660 nm laser was more efficient in neurofunctional recovery, and the Raman spectra of lipid and protein properties were attributed to the basic biochemical composition of the sciatic nerve.</description><subject>Animal models</subject><subject>Animals</subject><subject>Biochemical composition</subject><subject>Cell proliferation</subject><subject>Collagen</subject><subject>Compression</subject><subject>Dentistry</subject><subject>Gait</subject><subject>Glycoproteins</subject><subject>Injuries</subject><subject>Injury analysis</subject><subject>Lasers</subject><subject>Lesions</subject><subject>Lipids</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Myelin</subject><subject>Nerves</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photonics</subject><subject>Principal components analysis</subject><subject>Quantum Optics</subject><subject>Raman spectra</subject><subject>Raman spectroscopy</subject><subject>Recovery</subject><subject>Recovery of function</subject><subject>Regeneration</subject><subject>Rehabilitation</subject><subject>Schwann cells</subject><subject>Sciatic nerve</subject><subject>Sensorimotor system</subject><subject>Sheaths</subject><subject>Spectroscopy</subject><subject>Spectrum 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spectroscopy and sciatic functional index (SFI) after low-level laser therapy (LLLT) in a rat sciatic nerve crush injury model</title><author>de Almeida Melo Maciel Mangueira, Melissa ; Caparelli-Dáquer, Egas ; Filho, Ozimo Pereira Gama ; de Assis, Diogo Souza Ferreira Rubim ; Sousa, Janyeid Karla Castro ; Lima, Willy Leite ; Pinheiro, Antonio Luiz Barbosa ; Silveira, Landulfo ; Mangueira, Nilton Maciel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-c98c30e4cbb85fcd1ce6d61f108bc8afb439995d403bc0d33393808ad1157d883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Biochemical composition</topic><topic>Cell proliferation</topic><topic>Collagen</topic><topic>Compression</topic><topic>Dentistry</topic><topic>Gait</topic><topic>Glycoproteins</topic><topic>Injuries</topic><topic>Injury analysis</topic><topic>Lasers</topic><topic>Lesions</topic><topic>Lipids</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Myelin</topic><topic>Nerves</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Original Article</topic><topic>Photonics</topic><topic>Principal components analysis</topic><topic>Quantum Optics</topic><topic>Raman spectra</topic><topic>Raman spectroscopy</topic><topic>Recovery</topic><topic>Recovery of function</topic><topic>Regeneration</topic><topic>Rehabilitation</topic><topic>Schwann cells</topic><topic>Sciatic nerve</topic><topic>Sensorimotor system</topic><topic>Sheaths</topic><topic>Spectroscopy</topic><topic>Spectrum analysis</topic><topic>Sphingolipids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Almeida Melo Maciel Mangueira, Melissa</creatorcontrib><creatorcontrib>Caparelli-Dáquer, Egas</creatorcontrib><creatorcontrib>Filho, Ozimo Pereira 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Med Sci</addtitle><date>2022-09-01</date><risdate>2022</risdate><volume>37</volume><issue>7</issue><spage>2957</spage><epage>2971</epage><pages>2957-2971</pages><issn>1435-604X</issn><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>Axonotmesis causes sensorimotor and neurofunctional deficits, and its regeneration can occur slowly or not occur if not treated appropriately. Low-level laser therapy (LLLT) promotes nerve regeneration with the proliferation of myelinating Schwann cells to recover the myelin sheath and the production of glycoproteins for endoneurium reconstruction. This study aimed to evaluate the effects of LLLT on sciatic nerve regeneration after compression injury by means of the sciatic functional index (SFI) and Raman spectroscopy (RS). For this, 64 Wistar rats were divided into two groups according to the length of treatment: 14 days (
n
= 32) and 21 days (
n
= 32). These two groups were subdivided into four sub-groups of eight animals each (control 1; control 2; laser 660 nm; laser 808 nm). All animals had surgical exposure to the sciatic nerve, and only control 1 did not suffer nerve damage. To cause the lesion in the sciatic nerve, compression was applied with a Kelly clamp for 6 s. The evaluation of sensory deficit was performed by the painful exteroceptive sensitivity (PES) and neuromotor tests by the SFI. Laser 660 nm and laser 808 nm sub-groups were irradiated daily (100 mW, 40 s, energy density of 133 J/cm
2
). The sciatic nerve segment was removed for RS analysis. The animals showed accentuated sensory and neurofunctional deficit after injury and their rehabilitation occurred more effectively in the sub-groups treated with 660 nm laser. Control 2 sub-group did not obtain functional recovery of gait. The RS identified sphingolipids (718, 1065, and 1440 cm
−1
) and collagen (700, 852, 1004, 1270, and 1660 cm
−1
) as biomolecular characteristics of sciatic nerves. Principal component analysis revealed important differences among sub-groups and a directly proportional correlation with SFI, mainly in the sub-group laser 660 nm treated for 21 days. In the axonotmesis-type lesion model presented herein, the 660 nm laser was more efficient in neurofunctional recovery, and the Raman spectra of lipid and protein properties were attributed to the basic biochemical composition of the sciatic nerve.</abstract><cop>London</cop><pub>Springer London</pub><pmid>35503388</pmid><doi>10.1007/s10103-022-03565-5</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-9138-9557</orcidid></addata></record> |
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| ispartof | Lasers in medical science, 2022-09, Vol.37 (7), p.2957-2971 |
| issn | 1435-604X 0268-8921 1435-604X |
| language | eng |
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| source | SpringerLink Journals |
| subjects | Animal models Animals Biochemical composition Cell proliferation Collagen Compression Dentistry Gait Glycoproteins Injuries Injury analysis Lasers Lesions Lipids Medicine Medicine & Public Health Myelin Nerves Optical Devices Optics Original Article Photonics Principal components analysis Quantum Optics Raman spectra Raman spectroscopy Recovery Recovery of function Regeneration Rehabilitation Schwann cells Sciatic nerve Sensorimotor system Sheaths Spectroscopy Spectrum analysis Sphingolipids |
| title | Raman spectroscopy and sciatic functional index (SFI) after low-level laser therapy (LLLT) in a rat sciatic nerve crush injury model |
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