Increased Yield of Residual γH2AX Foci in p53-Deficient Human Lung Carcinoma Cells Exposed to Subpicosecond Beams of Accelerated Electrons
We studied quantitative yield of residual (24 h post-irradiation) phosphorylated histone (γH2AX) foci as a marker of DNA double strand breaks in wild-type A549 and p53-deficient H1299 human lung carcinoma cells after exposure to subpicosecond (energy 4 MeV, pulse duration 400 fsec, peak dose rate du...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2022-04, Vol.172 (6), p.756-759 |
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| creator | Vorobyeva, N. Yu Babayan, N. S. Grigoryan, B. A. Sargsyan, A. A. Khondkaryan, L. G. Apresyan, L. S. Chigasova, A. K. Yashkina, E. I. Guryev, D. V. Rodneva, S. M. Tsishnatti, A. A. Fedotov, Yu. A. Arutyunyan, R. M. Osipov, A. N. |
| description | We studied quantitative yield of residual (24 h post-irradiation) phosphorylated histone (γH2AX) foci as a marker of DNA double strand breaks in wild-type A549 and p53-deficient H1299 human lung carcinoma cells after exposure to subpicosecond (energy 4 MeV, pulse duration 400 fsec, peak dose rate during the pulse 16 GGy/s) and quasi-continuous (energy 3.6 MeV) beams of accelerated electrons in a dose range of 0.5-10.0 Gy. The efficiency of pulse irradiation in A549 and H1299 cells assessed by the yield of residual foci was higher than the efficiency of quasi-continuous exposure by 1.8 and 5.3 times, respectively. Significant differences in quantitative yield of residual γH2AX foci between wild-type and p53-deficient cell lines were observed only after exposure to subpicosecond, but not quasi-continuous beams of accelerated electrons. |
| doi_str_mv | 10.1007/s10517-022-05472-9 |
| format | Article |
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Yu ; Babayan, N. S. ; Grigoryan, B. A. ; Sargsyan, A. A. ; Khondkaryan, L. G. ; Apresyan, L. S. ; Chigasova, A. K. ; Yashkina, E. I. ; Guryev, D. V. ; Rodneva, S. M. ; Tsishnatti, A. A. ; Fedotov, Yu. A. ; Arutyunyan, R. M. ; Osipov, A. N.</creator><creatorcontrib>Vorobyeva, N. Yu ; Babayan, N. S. ; Grigoryan, B. A. ; Sargsyan, A. A. ; Khondkaryan, L. G. ; Apresyan, L. S. ; Chigasova, A. K. ; Yashkina, E. I. ; Guryev, D. V. ; Rodneva, S. M. ; Tsishnatti, A. A. ; Fedotov, Yu. A. ; Arutyunyan, R. M. ; Osipov, A. N.</creatorcontrib><description>We studied quantitative yield of residual (24 h post-irradiation) phosphorylated histone (γH2AX) foci as a marker of DNA double strand breaks in wild-type A549 and p53-deficient H1299 human lung carcinoma cells after exposure to subpicosecond (energy 4 MeV, pulse duration 400 fsec, peak dose rate during the pulse 16 GGy/s) and quasi-continuous (energy 3.6 MeV) beams of accelerated electrons in a dose range of 0.5-10.0 Gy. The efficiency of pulse irradiation in A549 and H1299 cells assessed by the yield of residual foci was higher than the efficiency of quasi-continuous exposure by 1.8 and 5.3 times, respectively. Significant differences in quantitative yield of residual γH2AX foci between wild-type and p53-deficient cell lines were observed only after exposure to subpicosecond, but not quasi-continuous beams of accelerated electrons.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-022-05472-9</identifier><identifier>PMID: 35501643</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cancer ; Cell Biology ; DNA Breaks, Double-Stranded ; DNA damage ; DNA Repair ; Dosimetry ; Efficiency ; Electrons ; Energy ; Histones ; Histones - genetics ; Histones - metabolism ; Histones - radiation effects ; Humans ; Internal Medicine ; Laboratory Medicine ; Lung cancer ; Lung carcinoma ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Lung Neoplasms - radiotherapy ; p53 Protein ; Pathology ; Post-irradiation ; Radiation ; Science ; Statistical analysis ; Tumor Suppressor Protein p53 - deficiency ; Tumor Suppressor Protein p53 - genetics ; Tumor Suppressor Protein p53 - metabolism</subject><ispartof>Bulletin of experimental biology and medicine, 2022-04, Vol.172 (6), p.756-759</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>2022. 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Yu</creatorcontrib><creatorcontrib>Babayan, N. S.</creatorcontrib><creatorcontrib>Grigoryan, B. A.</creatorcontrib><creatorcontrib>Sargsyan, A. A.</creatorcontrib><creatorcontrib>Khondkaryan, L. G.</creatorcontrib><creatorcontrib>Apresyan, L. S.</creatorcontrib><creatorcontrib>Chigasova, A. K.</creatorcontrib><creatorcontrib>Yashkina, E. I.</creatorcontrib><creatorcontrib>Guryev, D. V.</creatorcontrib><creatorcontrib>Rodneva, S. M.</creatorcontrib><creatorcontrib>Tsishnatti, A. A.</creatorcontrib><creatorcontrib>Fedotov, Yu. A.</creatorcontrib><creatorcontrib>Arutyunyan, R. M.</creatorcontrib><creatorcontrib>Osipov, A. N.</creatorcontrib><title>Increased Yield of Residual γH2AX Foci in p53-Deficient Human Lung Carcinoma Cells Exposed to Subpicosecond Beams of Accelerated Electrons</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>We studied quantitative yield of residual (24 h post-irradiation) phosphorylated histone (γH2AX) foci as a marker of DNA double strand breaks in wild-type A549 and p53-deficient H1299 human lung carcinoma cells after exposure to subpicosecond (energy 4 MeV, pulse duration 400 fsec, peak dose rate during the pulse 16 GGy/s) and quasi-continuous (energy 3.6 MeV) beams of accelerated electrons in a dose range of 0.5-10.0 Gy. The efficiency of pulse irradiation in A549 and H1299 cells assessed by the yield of residual foci was higher than the efficiency of quasi-continuous exposure by 1.8 and 5.3 times, respectively. Significant differences in quantitative yield of residual γH2AX foci between wild-type and p53-deficient cell lines were observed only after exposure to subpicosecond, but not quasi-continuous beams of accelerated electrons.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer</subject><subject>Cell Biology</subject><subject>DNA Breaks, Double-Stranded</subject><subject>DNA damage</subject><subject>DNA Repair</subject><subject>Dosimetry</subject><subject>Efficiency</subject><subject>Electrons</subject><subject>Energy</subject><subject>Histones</subject><subject>Histones - genetics</subject><subject>Histones - metabolism</subject><subject>Histones - radiation effects</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Lung cancer</subject><subject>Lung carcinoma</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - radiotherapy</subject><subject>p53 Protein</subject><subject>Pathology</subject><subject>Post-irradiation</subject><subject>Radiation</subject><subject>Science</subject><subject>Statistical analysis</subject><subject>Tumor Suppressor Protein p53 - deficiency</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kcuKFDEUhoMoTtv6Ai4k4MZNNPeklm3bYw80CF5AV0U6dWrIUJWUSRXoM_g4vofPZNoeFVy4Cod85_8PfAg9ZvQ5o9S8KIwqZgjlnFAlDSfNHbRiyghiOWd30YpWikhr7QV6UMrNaaSa3UcXQinKtBQr9O0q-gyuQIc_BRg6nHr8FkroFjfgH9_3fPMRXyYfcIh4UoK8gj74AHHG-2V0ER-WeI23LvsQ0-jwFoah4N2XKZ0S54TfLccp-Dr5FDv8EtxYThUb72GA7OZK7Qbwc06xPET3ejcUeHT7rtGHy9377Z4c3ry-2m4OxAujZtIo1VnDDUhvXAMMLNVWaWokSCUZNaB676QWDoTpnZDAetvbRutGu4YexRo9O-dOOX1eoMztGEq9Z3AR0lJarlXDuZUNr-jTf9CbtORYr6uUVkJrVovWiJ8pn1MpGfp2ymF0-WvLaHtS1Z5VtVVV-0tV29SlJ7fRy3GE7s_KbzcVEGeg1K94Dflv939ifwJiRJ3x</recordid><startdate>20220401</startdate><enddate>20220401</enddate><creator>Vorobyeva, N. 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Yu ; Babayan, N. S. ; Grigoryan, B. A. ; Sargsyan, A. A. ; Khondkaryan, L. G. ; Apresyan, L. S. ; Chigasova, A. K. ; Yashkina, E. I. ; Guryev, D. V. ; Rodneva, S. M. ; Tsishnatti, A. A. ; Fedotov, Yu. A. ; Arutyunyan, R. M. ; Osipov, A. 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The efficiency of pulse irradiation in A549 and H1299 cells assessed by the yield of residual foci was higher than the efficiency of quasi-continuous exposure by 1.8 and 5.3 times, respectively. Significant differences in quantitative yield of residual γH2AX foci between wild-type and p53-deficient cell lines were observed only after exposure to subpicosecond, but not quasi-continuous beams of accelerated electrons.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35501643</pmid><doi>10.1007/s10517-022-05472-9</doi><tpages>4</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Cancer Cell Biology DNA Breaks, Double-Stranded DNA damage DNA Repair Dosimetry Efficiency Electrons Energy Histones Histones - genetics Histones - metabolism Histones - radiation effects Humans Internal Medicine Laboratory Medicine Lung cancer Lung carcinoma Lung Neoplasms - genetics Lung Neoplasms - metabolism Lung Neoplasms - radiotherapy p53 Protein Pathology Post-irradiation Radiation Science Statistical analysis Tumor Suppressor Protein p53 - deficiency Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism |
| title | Increased Yield of Residual γH2AX Foci in p53-Deficient Human Lung Carcinoma Cells Exposed to Subpicosecond Beams of Accelerated Electrons |
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