In vitro characterization of primary osteoblasts on titanium surfaces processed with wire-type electric discharge machining
The fixation of titanium implants in bone tissue is affected by the presence of a passive titanium oxide (TiO2) layer. Specifically, oxidation products in the amorphous TiO2 matrix enhance the mechanical properties of mineralized tissues. In addition, in vitro mineralization mediated by primary oste...
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| Veröffentlicht in: | Dental Materials Journal 2022/09/25, Vol.41(5), pp.655-659 |
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| creator | KUROSAKA, Masao KATAOKA, Yu MIYAZAKI, Takashi |
| description | The fixation of titanium implants in bone tissue is affected by the presence of a passive titanium oxide (TiO2) layer. Specifically, oxidation products in the amorphous TiO2 matrix enhance the mechanical properties of mineralized tissues. In addition, in vitro mineralization mediated by primary osteoblasts on amorphous TiO2 generates stiff tissues in a process that resembles pathological mechanisms connected with tumors and proceeds through hydrogen peroxide-inducible clone-5 (Hic-5) expression. However, the relationship between surface-based peroxidation and stiff mineralized tissue formation remains unclear. In this study, titanium samples were processed using wire electrical discharge machining to generate oxidation products in amorphous TiO2. The gene expression profiles of primary osteoblasts cultured on these specimens were characterized. Increased expression of Hic-5 was correlated with the presence of peroxidation products. The crystallization of amorphous TiO2 in these samples reduced the expression of both Hic-5 and lysyl oxidase, an enzyme that promotes matrix cross-linking. |
| doi_str_mv | 10.4012/dmj.2021-231 |
| format | Article |
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Specifically, oxidation products in the amorphous TiO2 matrix enhance the mechanical properties of mineralized tissues. In addition, in vitro mineralization mediated by primary osteoblasts on amorphous TiO2 generates stiff tissues in a process that resembles pathological mechanisms connected with tumors and proceeds through hydrogen peroxide-inducible clone-5 (Hic-5) expression. However, the relationship between surface-based peroxidation and stiff mineralized tissue formation remains unclear. In this study, titanium samples were processed using wire electrical discharge machining to generate oxidation products in amorphous TiO2. The gene expression profiles of primary osteoblasts cultured on these specimens were characterized. Increased expression of Hic-5 was correlated with the presence of peroxidation products. The crystallization of amorphous TiO2 in these samples reduced the expression of both Hic-5 and lysyl oxidase, an enzyme that promotes matrix cross-linking.</description><identifier>ISSN: 0287-4547</identifier><identifier>EISSN: 1881-1361</identifier><identifier>DOI: 10.4012/dmj.2021-231</identifier><identifier>PMID: 35491106</identifier><language>eng</language><publisher>Japan: The Japanese Society for Dental Materials and Devices</publisher><subject>Bone implants ; Crosslinking ; Crystallization ; Electric discharge machining ; Gene expression ; Hydrogen peroxide ; Hydrogen peroxide-inducible clone-5 ; Lysyl oxidase ; Mechanical properties ; Mineralization ; Osteoblast ; Osteoblasts ; Oxidation ; PCR ; Peroxidation ; Tissues ; Titanium ; Titanium dioxide ; Titanium oxide ; Titanium oxides ; Tumors ; Wire ; Wire electrical discharge machining</subject><ispartof>Dental Materials Journal, 2022/09/25, Vol.41(5), pp.655-659</ispartof><rights>2022 The Japanese Society for Dental Materials and Devices</rights><rights>Copyright Japan Science and Technology Agency 2022</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c463t-265fe4220bb7723fa3924f3e5d8f2c34334061c9d81de0230066013f148172a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,1887,27933,27934</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35491106$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KUROSAKA, Masao</creatorcontrib><creatorcontrib>KATAOKA, Yu</creatorcontrib><creatorcontrib>MIYAZAKI, Takashi</creatorcontrib><title>In vitro characterization of primary osteoblasts on titanium surfaces processed with wire-type electric discharge machining</title><title>Dental Materials Journal</title><addtitle>Dent. Mater. J.</addtitle><description>The fixation of titanium implants in bone tissue is affected by the presence of a passive titanium oxide (TiO2) layer. Specifically, oxidation products in the amorphous TiO2 matrix enhance the mechanical properties of mineralized tissues. In addition, in vitro mineralization mediated by primary osteoblasts on amorphous TiO2 generates stiff tissues in a process that resembles pathological mechanisms connected with tumors and proceeds through hydrogen peroxide-inducible clone-5 (Hic-5) expression. However, the relationship between surface-based peroxidation and stiff mineralized tissue formation remains unclear. In this study, titanium samples were processed using wire electrical discharge machining to generate oxidation products in amorphous TiO2. The gene expression profiles of primary osteoblasts cultured on these specimens were characterized. Increased expression of Hic-5 was correlated with the presence of peroxidation products. The crystallization of amorphous TiO2 in these samples reduced the expression of both Hic-5 and lysyl oxidase, an enzyme that promotes matrix cross-linking.</description><subject>Bone implants</subject><subject>Crosslinking</subject><subject>Crystallization</subject><subject>Electric discharge machining</subject><subject>Gene expression</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen peroxide-inducible clone-5</subject><subject>Lysyl oxidase</subject><subject>Mechanical properties</subject><subject>Mineralization</subject><subject>Osteoblast</subject><subject>Osteoblasts</subject><subject>Oxidation</subject><subject>PCR</subject><subject>Peroxidation</subject><subject>Tissues</subject><subject>Titanium</subject><subject>Titanium dioxide</subject><subject>Titanium oxide</subject><subject>Titanium oxides</subject><subject>Tumors</subject><subject>Wire</subject><subject>Wire electrical discharge machining</subject><issn>0287-4547</issn><issn>1881-1361</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpdkTFvFDEQhS0EIkego0aWaCiyicf2en0lRAQiRaIJteXzju982l0H2wsK_Hm83OUKmpliPr15eo-Qt8AuJQN-1Y_7S844NFzAM7ICraEBoeA5WTGuu0a2sjsjr3LeMybXSuuX5Ey0cg3A1Ir8uZ3oz1BSpG5nk3UFU_htS4gTjZ4-pDDa9EhjLhg3g80l03opodgpzCPNc_LWYa5grCtjT3-FsqsjYVMeH5DigK6k4Ggf8vJhi3S0bhemMG1fkxfeDhnfHPc5-X7z-f76a3P37cvt9ce7xkklSsNV61FyzjabruPCW7Hm0gtse-25E1IIyRS4da-hR8YFY0oxEB6kho5bIc7Jh4NudfljxlzMWM3gMNgJ45xNfaCV7CTIir7_D93HOU3VneEdr3FDRSt1caBcijkn9OYYlAFmllJMLcUspZhaSsXfHUXnzYj9CX5qoQKfDsA-F7vFE2BTCW7Af2oSTLuMJ9XTcUnV4CT-Al53n_Y</recordid><startdate>20220925</startdate><enddate>20220925</enddate><creator>KUROSAKA, Masao</creator><creator>KATAOKA, Yu</creator><creator>MIYAZAKI, Takashi</creator><general>The Japanese Society for Dental Materials and Devices</general><general>Japan Science and Technology Agency</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20220925</creationdate><title>In vitro characterization of primary osteoblasts on titanium surfaces processed with wire-type electric discharge machining</title><author>KUROSAKA, Masao ; 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Mater. J.</addtitle><date>2022-09-25</date><risdate>2022</risdate><volume>41</volume><issue>5</issue><spage>655</spage><epage>659</epage><pages>655-659</pages><artnum>2021-231</artnum><issn>0287-4547</issn><eissn>1881-1361</eissn><abstract>The fixation of titanium implants in bone tissue is affected by the presence of a passive titanium oxide (TiO2) layer. Specifically, oxidation products in the amorphous TiO2 matrix enhance the mechanical properties of mineralized tissues. In addition, in vitro mineralization mediated by primary osteoblasts on amorphous TiO2 generates stiff tissues in a process that resembles pathological mechanisms connected with tumors and proceeds through hydrogen peroxide-inducible clone-5 (Hic-5) expression. However, the relationship between surface-based peroxidation and stiff mineralized tissue formation remains unclear. In this study, titanium samples were processed using wire electrical discharge machining to generate oxidation products in amorphous TiO2. 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| ispartof | Dental Materials Journal, 2022/09/25, Vol.41(5), pp.655-659 |
| issn | 0287-4547 1881-1361 |
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| source | EZB Electronic Journals Library; J-STAGE |
| subjects | Bone implants Crosslinking Crystallization Electric discharge machining Gene expression Hydrogen peroxide Hydrogen peroxide-inducible clone-5 Lysyl oxidase Mechanical properties Mineralization Osteoblast Osteoblasts Oxidation PCR Peroxidation Tissues Titanium Titanium dioxide Titanium oxide Titanium oxides Tumors Wire Wire electrical discharge machining |
| title | In vitro characterization of primary osteoblasts on titanium surfaces processed with wire-type electric discharge machining |
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