Structural and biochemical analyses of the nucleosome containing Komagataella pastoris histones

Abstract Komagataella pastoris is a methylotrophic yeast that is commonly used as a host cell for protein production. In the present study, we reconstituted the nucleosome with K. pastoris histones and determined the structure of the nucleosome core particle by cryogenic electron microscopy. In the...

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Veröffentlicht in:Journal of biochemistry (Tokyo) 2022-07, Vol.172 (2), p.79-88
Hauptverfasser: Fukushima, Yutaro, Hatazawa, Suguru, Hirai, Seiya, Kujirai, Tomoya, Ehara, Haruhiko, Sekine, Shun-ichi, Takizawa, Yoshimasa, Kurumizaka, Hitoshi
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Sprache:eng
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