Molecular cloning, expression in Escherichia coli and structural-functional analysis of a pyruvate kinase from Pyrobaculum calidifontis

This manuscript describes recombinant production, characterization and structural analysis of wild-type and mutant Pcal_0029, a pyruvate kinase from Pyrobaculum calidifontis. Recombinant Pcal_0029 was produced in soluble and highly active form in Escherichia coli. Purified protein exhibited divalent...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	International journal of biological macromolecules 2022-06, Vol.209 (Pt A), p.1410-1421
Hauptverfasser:	Falak, Samia, Saeed, Muhammad Sulaiman, Rashid, Naeem
Format:	Artikel
Sprache:	eng
Schlagworte:	Archaeal Proteins - chemistry Cloning, Molecular Enzyme Stability Escherichia coli - genetics Escherichia coli - metabolism Hyperthermophile Mutations Pyrobaculum - genetics Pyrobaculum calidifontis Pyruvate kinase Pyruvate Kinase - genetics Pyruvate Kinase - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism Structural analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!