Trehalose accumulation and radiation resistance due to prior heat stress in Saccharomyces cerevisiae
In this study, we examined the accumulation of trehalose, a stress-responsive substance, upon gamma-ray irradiation by evaluating the cause of trehalose accumulation and the development of gamma-ray resistance through intracellular trehalose accumulation. Saccharomyces cerevisiae cells cultured to t...
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| Veröffentlicht in: | Archives of microbiology 2022-05, Vol.204 (5), p.275-275, Article 275 |
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| creator | Asada, Ryoko Watanabe, Takeru Tanaka, Yoshiharu Kishida, Masao Furuta, Masakazu |
| description | In this study, we examined the accumulation of trehalose, a stress-responsive substance, upon gamma-ray irradiation by evaluating the cause of trehalose accumulation and the development of gamma-ray resistance through intracellular trehalose accumulation.
Saccharomyces cerevisiae
cells cultured to the logarithmic growth phase were irradiated with gamma rays, and the intracellular trehalose content was measured. However, trehalose was not detectable. The yeast cells with trehalose accumulation caused by pre-treatment at 40 °C were irradiated with gamma rays, and the resistance of these cells to gamma radiation was compared with that of cells without heat treatment. Trehalose accumulation resulted in gamma-ray resistance and suppressed the increase in reactive oxygen species, lipid peroxidation, and DNA double-strand break production in yeast cells. The tests were also performed with a trehalose-6-phosphate-synthase (TPS1)-deficient mutant strain (Δ
tps1
) unable to synthesize trehalose, and the results revealed that
TPS1
was involved in protection against oxidative stress. |
| doi_str_mv | 10.1007/s00203-022-02892-z |
| format | Article |
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Saccharomyces cerevisiae
cells cultured to the logarithmic growth phase were irradiated with gamma rays, and the intracellular trehalose content was measured. However, trehalose was not detectable. The yeast cells with trehalose accumulation caused by pre-treatment at 40 °C were irradiated with gamma rays, and the resistance of these cells to gamma radiation was compared with that of cells without heat treatment. Trehalose accumulation resulted in gamma-ray resistance and suppressed the increase in reactive oxygen species, lipid peroxidation, and DNA double-strand break production in yeast cells. The tests were also performed with a trehalose-6-phosphate-synthase (TPS1)-deficient mutant strain (Δ
tps1
) unable to synthesize trehalose, and the results revealed that
TPS1
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Saccharomyces cerevisiae
cells cultured to the logarithmic growth phase were irradiated with gamma rays, and the intracellular trehalose content was measured. However, trehalose was not detectable. The yeast cells with trehalose accumulation caused by pre-treatment at 40 °C were irradiated with gamma rays, and the resistance of these cells to gamma radiation was compared with that of cells without heat treatment. Trehalose accumulation resulted in gamma-ray resistance and suppressed the increase in reactive oxygen species, lipid peroxidation, and DNA double-strand break production in yeast cells. The tests were also performed with a trehalose-6-phosphate-synthase (TPS1)-deficient mutant strain (Δ
tps1
) unable to synthesize trehalose, and the results revealed that
TPS1
was involved in protection against oxidative stress.</description><subject>Accumulation</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Deficient mutant</subject><subject>DNA damage</subject><subject>Ecology</subject><subject>Gamma rays</subject><subject>Heat stress</subject><subject>Heat tolerance</subject><subject>Heat treatment</subject><subject>Heat treatments</subject><subject>Intracellular</subject><subject>Irradiation</subject><subject>Life Sciences</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Oxidative stress</subject><subject>Peroxidation</subject><subject>Radiation</subject><subject>Radiation tolerance</subject><subject>Reactive oxygen species</subject><subject>Saccharomyces cerevisiae</subject><subject>Trehalose</subject><subject>Trehalose-6-phosphate</subject><subject>Yeast</subject><subject>Yeasts</subject><subject>γ 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Ecology</topic><topic>Microbiology</topic><topic>Original Paper</topic><topic>Oxidative stress</topic><topic>Peroxidation</topic><topic>Radiation</topic><topic>Radiation tolerance</topic><topic>Reactive oxygen species</topic><topic>Saccharomyces cerevisiae</topic><topic>Trehalose</topic><topic>Trehalose-6-phosphate</topic><topic>Yeast</topic><topic>Yeasts</topic><topic>γ Radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asada, Ryoko</creatorcontrib><creatorcontrib>Watanabe, Takeru</creatorcontrib><creatorcontrib>Tanaka, Yoshiharu</creatorcontrib><creatorcontrib>Kishida, Masao</creatorcontrib><creatorcontrib>Furuta, Masakazu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical 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Microbiol</addtitle><date>2022-05-01</date><risdate>2022</risdate><volume>204</volume><issue>5</issue><spage>275</spage><epage>275</epage><pages>275-275</pages><artnum>275</artnum><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>In this study, we examined the accumulation of trehalose, a stress-responsive substance, upon gamma-ray irradiation by evaluating the cause of trehalose accumulation and the development of gamma-ray resistance through intracellular trehalose accumulation.
Saccharomyces cerevisiae
cells cultured to the logarithmic growth phase were irradiated with gamma rays, and the intracellular trehalose content was measured. However, trehalose was not detectable. The yeast cells with trehalose accumulation caused by pre-treatment at 40 °C were irradiated with gamma rays, and the resistance of these cells to gamma radiation was compared with that of cells without heat treatment. Trehalose accumulation resulted in gamma-ray resistance and suppressed the increase in reactive oxygen species, lipid peroxidation, and DNA double-strand break production in yeast cells. The tests were also performed with a trehalose-6-phosphate-synthase (TPS1)-deficient mutant strain (Δ
tps1
) unable to synthesize trehalose, and the results revealed that
TPS1
was involved in protection against oxidative stress.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>35451658</pmid><doi>10.1007/s00203-022-02892-z</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-0626-1482</orcidid></addata></record> |
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| ispartof | Archives of microbiology, 2022-05, Vol.204 (5), p.275-275, Article 275 |
| issn | 0302-8933 1432-072X |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Accumulation Biochemistry Biomedical and Life Sciences Biotechnology Cell Biology Deficient mutant DNA damage Ecology Gamma rays Heat stress Heat tolerance Heat treatment Heat treatments Intracellular Irradiation Life Sciences Lipid peroxidation Lipids Microbial Ecology Microbiology Original Paper Oxidative stress Peroxidation Radiation Radiation tolerance Reactive oxygen species Saccharomyces cerevisiae Trehalose Trehalose-6-phosphate Yeast Yeasts γ Radiation |
| title | Trehalose accumulation and radiation resistance due to prior heat stress in Saccharomyces cerevisiae |
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