Rhizospheric plant-microbe synergistic interactions achieve efficient arsenic phytoextraction by Pteris vittata

Phytoextraction is a cost-effective and eco-friendly technology to remove arsenic (As) from contaminated soil using plants and associated microorganisms. Pteris vittata is the most studied As hyperaccumulator, which effectively takes up inorganic arsenate via roots. Arsenic solubilization and speciation occur prior to plant absorption in the rhizosphere, which play a key role in As phytoextraction by P. vittata. This study investigated the metabolomic correlation of P. vittata and associated rhizospheric microorganisms during As phytoextraction. Three-month pot cultivation of P. vittata in As polluted soil was conducted. In rhizosphere, an increase of water-soluble As concentration and a decrease of pH was observed in the second month, suggesting acidic metabolites as a possible cause of As solubilization. A correlation network was built to elucidate the interactions among metabolites, bacteria and fungi in the rhizosphere of P. vittata. Our results demonstrate that the plant is the major driving force of rhizospheric microbiota generation, and both microbial community and metabolites in rhizosphere of P. vittata correlate to increased bioavailable As. Multi-omics analysis revealed that pterosins enrich microbes that potentially promote As phytoextraction. This study extends the current view of rhizospheric plant-microbes synergistic effects of hyperaccumulators on phytoextraction, which provides clues for developing efficient As phytoremediation approaches. [Display omitted] •Pteris vittata decreased the pH value of rhizospheric soil within two months.•Rhizospheric microbiome and metabolome correlated to increasing bioavailable As.•Secreted metabolites shaped rhizospheric bacteria & fungi community of P. vittata.•Pterosins positively associated with microbes which promote As phytoextraction.•Pterosins in rhizosphere of P. vittata positively correlated to bioavailable As.