Long Non-coding RNA LOXL1-AS1 Facilitates Colorectal Cancer Progression via Regulating miR-1224-5p/miR-761/HK2 Axis
Colorectal cancer (CRC) is one of the most common cancers worldwide. Long non-coding RNAs (lncRNAs) play crucial roles in the development of malignant tumors. The present study aimed to explore the function and potential mechanism of lncRNA LOXL1 antisense RNA 1 (LOXL1-AS1) in CRC. The abundance of...
Gespeichert in:
| Veröffentlicht in: | Biochemical genetics 2022-12, Vol.60 (6), p.2416-2433 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2433 |
|---|---|
| container_issue | 6 |
| container_start_page | 2416 |
| container_title | Biochemical genetics |
| container_volume | 60 |
| creator | Guo, Tao Peng, Shihao Liu, Defeng Li, Yangyang |
| description | Colorectal cancer (CRC) is one of the most common cancers worldwide. Long non-coding RNAs (lncRNAs) play crucial roles in the development of malignant tumors. The present study aimed to explore the function and potential mechanism of lncRNA LOXL1 antisense RNA 1 (LOXL1-AS1) in CRC. The abundance of LOXL1-AS1, miR-1224-5p, miR-761, and hexokinase 2 (HK2) was detected by quantitative real-time PCR or western blot assay. Cell proliferation was assessed by Cell Counting Kit-8 and colony formation assays. Cell apoptosis, invasion, and migration were examined by flow cytometry, transwell assay, and wound healing assay. Glycolysis was evaluated by detecting glucose consumption, lactate production, and ATP/ADP ratios. Xenograft assay was used for in vivo tumor growth analysis. LOXL1-AS1 and HK2 levels were increased, while miR-1224-5p and miR-761 levels were reduced in CRC tissues and cells. Knockdown of LOXL1-AS1 suppressed CRC cell proliferation, invasion, migration, and glycolysis, and induced cell apoptosis. Silencing of LOXL1-AS1 blocked tumor growth in vivo. Moreover, LOXL1-AS1 accelerated CRC cell progression by absorbing miR-1224-5p/miR-761. Besides, miR-1224-5p and miR-761 inhibited CRC cell progression via targeting HK2. LOXL1-AS1 contributed to CRC progression via modulating miR-1224-5p/miR-761/HK2 pathway. |
| doi_str_mv | 10.1007/s10528-022-10226-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2652863051</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2729996817</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-1752fe5a7016b2154cb376556c88717421361439bf2c631c3a5eaf4b28358d623</originalsourceid><addsrcrecordid>eNp9kU1vEzEQhi1ERUPhD3BAlrhwMfGMv3aPUUQpYtWiABI3y-t4o60262DvIvj3OKRQqYde7LH8zDuWH0JeAX8HnJtlBq6wYhyRQVk0E0_IApQRTNZonpIF51wzrLE6J89zvi3Hmkv5jJwLJSXUSixIbuK4o9dxZD5u-1Jurle0ufneAFt9AXrpfD_0k5tCpus4xBT85Aa6dqMPiX5OcZdCzn0c6c_e0U3YzYObjjH7fsMAUTJ1WB5ro2F59Qnp6lefX5Czzg05vLzbL8i3y_df11esufnwcb1qmBdGTQyMwi4oZzjoFkFJ3wqjldK-qgwYiSA0SFG3HXotwAungutki5VQ1VajuCBvT7mHFH_MIU9232cfhsGNIc7Zoi7fpwVXUNA3D9DbOKexvM6iwbqudQWmUHiifIo5p9DZQ-r3Lv22wO1RiT0pscWG_avEitL0-i56bvdh-7_ln4MCiBOQy9W4C-l-9iOxfwBZNJEw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2729996817</pqid></control><display><type>article</type><title>Long Non-coding RNA LOXL1-AS1 Facilitates Colorectal Cancer Progression via Regulating miR-1224-5p/miR-761/HK2 Axis</title><source>SpringerLink Journals</source><creator>Guo, Tao ; Peng, Shihao ; Liu, Defeng ; Li, Yangyang</creator><creatorcontrib>Guo, Tao ; Peng, Shihao ; Liu, Defeng ; Li, Yangyang</creatorcontrib><description>Colorectal cancer (CRC) is one of the most common cancers worldwide. Long non-coding RNAs (lncRNAs) play crucial roles in the development of malignant tumors. The present study aimed to explore the function and potential mechanism of lncRNA LOXL1 antisense RNA 1 (LOXL1-AS1) in CRC. The abundance of LOXL1-AS1, miR-1224-5p, miR-761, and hexokinase 2 (HK2) was detected by quantitative real-time PCR or western blot assay. Cell proliferation was assessed by Cell Counting Kit-8 and colony formation assays. Cell apoptosis, invasion, and migration were examined by flow cytometry, transwell assay, and wound healing assay. Glycolysis was evaluated by detecting glucose consumption, lactate production, and ATP/ADP ratios. Xenograft assay was used for in vivo tumor growth analysis. LOXL1-AS1 and HK2 levels were increased, while miR-1224-5p and miR-761 levels were reduced in CRC tissues and cells. Knockdown of LOXL1-AS1 suppressed CRC cell proliferation, invasion, migration, and glycolysis, and induced cell apoptosis. Silencing of LOXL1-AS1 blocked tumor growth in vivo. Moreover, LOXL1-AS1 accelerated CRC cell progression by absorbing miR-1224-5p/miR-761. Besides, miR-1224-5p and miR-761 inhibited CRC cell progression via targeting HK2. LOXL1-AS1 contributed to CRC progression via modulating miR-1224-5p/miR-761/HK2 pathway.</description><identifier>ISSN: 0006-2928</identifier><identifier>EISSN: 1573-4927</identifier><identifier>DOI: 10.1007/s10528-022-10226-3</identifier><identifier>PMID: 35441953</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenosine diphosphate ; Antisense RNA ; Apoptosis ; Assaying ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cancer ; Cell growth ; Cell migration ; Cell proliferation ; Color coding ; Colorectal cancer ; Colorectal carcinoma ; Flow cytometry ; Glycolysis ; Hexokinase ; Human Genetics ; Lactic acid ; Medical Microbiology ; Non-coding RNA ; Original Article ; Tumors ; Wound healing ; Xenografts ; Xenotransplantation ; Zoology</subject><ispartof>Biochemical genetics, 2022-12, Vol.60 (6), p.2416-2433</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-1752fe5a7016b2154cb376556c88717421361439bf2c631c3a5eaf4b28358d623</citedby><cites>FETCH-LOGICAL-c375t-1752fe5a7016b2154cb376556c88717421361439bf2c631c3a5eaf4b28358d623</cites><orcidid>0000-0002-5852-1242</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10528-022-10226-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10528-022-10226-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35441953$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Tao</creatorcontrib><creatorcontrib>Peng, Shihao</creatorcontrib><creatorcontrib>Liu, Defeng</creatorcontrib><creatorcontrib>Li, Yangyang</creatorcontrib><title>Long Non-coding RNA LOXL1-AS1 Facilitates Colorectal Cancer Progression via Regulating miR-1224-5p/miR-761/HK2 Axis</title><title>Biochemical genetics</title><addtitle>Biochem Genet</addtitle><addtitle>Biochem Genet</addtitle><description>Colorectal cancer (CRC) is one of the most common cancers worldwide. Long non-coding RNAs (lncRNAs) play crucial roles in the development of malignant tumors. The present study aimed to explore the function and potential mechanism of lncRNA LOXL1 antisense RNA 1 (LOXL1-AS1) in CRC. The abundance of LOXL1-AS1, miR-1224-5p, miR-761, and hexokinase 2 (HK2) was detected by quantitative real-time PCR or western blot assay. Cell proliferation was assessed by Cell Counting Kit-8 and colony formation assays. Cell apoptosis, invasion, and migration were examined by flow cytometry, transwell assay, and wound healing assay. Glycolysis was evaluated by detecting glucose consumption, lactate production, and ATP/ADP ratios. Xenograft assay was used for in vivo tumor growth analysis. LOXL1-AS1 and HK2 levels were increased, while miR-1224-5p and miR-761 levels were reduced in CRC tissues and cells. Knockdown of LOXL1-AS1 suppressed CRC cell proliferation, invasion, migration, and glycolysis, and induced cell apoptosis. Silencing of LOXL1-AS1 blocked tumor growth in vivo. Moreover, LOXL1-AS1 accelerated CRC cell progression by absorbing miR-1224-5p/miR-761. Besides, miR-1224-5p and miR-761 inhibited CRC cell progression via targeting HK2. LOXL1-AS1 contributed to CRC progression via modulating miR-1224-5p/miR-761/HK2 pathway.</description><subject>Adenosine diphosphate</subject><subject>Antisense RNA</subject><subject>Apoptosis</subject><subject>Assaying</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Color coding</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Flow cytometry</subject><subject>Glycolysis</subject><subject>Hexokinase</subject><subject>Human Genetics</subject><subject>Lactic acid</subject><subject>Medical Microbiology</subject><subject>Non-coding RNA</subject><subject>Original Article</subject><subject>Tumors</subject><subject>Wound healing</subject><subject>Xenografts</subject><subject>Xenotransplantation</subject><subject>Zoology</subject><issn>0006-2928</issn><issn>1573-4927</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU1vEzEQhi1ERUPhD3BAlrhwMfGMv3aPUUQpYtWiABI3y-t4o60262DvIvj3OKRQqYde7LH8zDuWH0JeAX8HnJtlBq6wYhyRQVk0E0_IApQRTNZonpIF51wzrLE6J89zvi3Hmkv5jJwLJSXUSixIbuK4o9dxZD5u-1Jurle0ufneAFt9AXrpfD_0k5tCpus4xBT85Aa6dqMPiX5OcZdCzn0c6c_e0U3YzYObjjH7fsMAUTJ1WB5ro2F59Qnp6lefX5Czzg05vLzbL8i3y_df11esufnwcb1qmBdGTQyMwi4oZzjoFkFJ3wqjldK-qgwYiSA0SFG3HXotwAungutki5VQ1VajuCBvT7mHFH_MIU9232cfhsGNIc7Zoi7fpwVXUNA3D9DbOKexvM6iwbqudQWmUHiifIo5p9DZQ-r3Lv22wO1RiT0pscWG_avEitL0-i56bvdh-7_ln4MCiBOQy9W4C-l-9iOxfwBZNJEw</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Guo, Tao</creator><creator>Peng, Shihao</creator><creator>Liu, Defeng</creator><creator>Li, Yangyang</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5852-1242</orcidid></search><sort><creationdate>20221201</creationdate><title>Long Non-coding RNA LOXL1-AS1 Facilitates Colorectal Cancer Progression via Regulating miR-1224-5p/miR-761/HK2 Axis</title><author>Guo, Tao ; Peng, Shihao ; Liu, Defeng ; Li, Yangyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-1752fe5a7016b2154cb376556c88717421361439bf2c631c3a5eaf4b28358d623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adenosine diphosphate</topic><topic>Antisense RNA</topic><topic>Apoptosis</topic><topic>Assaying</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer</topic><topic>Cell growth</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Color coding</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Flow cytometry</topic><topic>Glycolysis</topic><topic>Hexokinase</topic><topic>Human Genetics</topic><topic>Lactic acid</topic><topic>Medical Microbiology</topic><topic>Non-coding RNA</topic><topic>Original Article</topic><topic>Tumors</topic><topic>Wound healing</topic><topic>Xenografts</topic><topic>Xenotransplantation</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Tao</creatorcontrib><creatorcontrib>Peng, Shihao</creatorcontrib><creatorcontrib>Liu, Defeng</creatorcontrib><creatorcontrib>Li, Yangyang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Tao</au><au>Peng, Shihao</au><au>Liu, Defeng</au><au>Li, Yangyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long Non-coding RNA LOXL1-AS1 Facilitates Colorectal Cancer Progression via Regulating miR-1224-5p/miR-761/HK2 Axis</atitle><jtitle>Biochemical genetics</jtitle><stitle>Biochem Genet</stitle><addtitle>Biochem Genet</addtitle><date>2022-12-01</date><risdate>2022</risdate><volume>60</volume><issue>6</issue><spage>2416</spage><epage>2433</epage><pages>2416-2433</pages><issn>0006-2928</issn><eissn>1573-4927</eissn><abstract>Colorectal cancer (CRC) is one of the most common cancers worldwide. Long non-coding RNAs (lncRNAs) play crucial roles in the development of malignant tumors. The present study aimed to explore the function and potential mechanism of lncRNA LOXL1 antisense RNA 1 (LOXL1-AS1) in CRC. The abundance of LOXL1-AS1, miR-1224-5p, miR-761, and hexokinase 2 (HK2) was detected by quantitative real-time PCR or western blot assay. Cell proliferation was assessed by Cell Counting Kit-8 and colony formation assays. Cell apoptosis, invasion, and migration were examined by flow cytometry, transwell assay, and wound healing assay. Glycolysis was evaluated by detecting glucose consumption, lactate production, and ATP/ADP ratios. Xenograft assay was used for in vivo tumor growth analysis. LOXL1-AS1 and HK2 levels were increased, while miR-1224-5p and miR-761 levels were reduced in CRC tissues and cells. Knockdown of LOXL1-AS1 suppressed CRC cell proliferation, invasion, migration, and glycolysis, and induced cell apoptosis. Silencing of LOXL1-AS1 blocked tumor growth in vivo. Moreover, LOXL1-AS1 accelerated CRC cell progression by absorbing miR-1224-5p/miR-761. Besides, miR-1224-5p and miR-761 inhibited CRC cell progression via targeting HK2. LOXL1-AS1 contributed to CRC progression via modulating miR-1224-5p/miR-761/HK2 pathway.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35441953</pmid><doi>10.1007/s10528-022-10226-3</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0002-5852-1242</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2928 |
| ispartof | Biochemical genetics, 2022-12, Vol.60 (6), p.2416-2433 |
| issn | 0006-2928 1573-4927 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2652863051 |
| source | SpringerLink Journals |
| subjects | Adenosine diphosphate Antisense RNA Apoptosis Assaying Biochemistry Biomedical and Life Sciences Biomedicine Cancer Cell growth Cell migration Cell proliferation Color coding Colorectal cancer Colorectal carcinoma Flow cytometry Glycolysis Hexokinase Human Genetics Lactic acid Medical Microbiology Non-coding RNA Original Article Tumors Wound healing Xenografts Xenotransplantation Zoology |
| title | Long Non-coding RNA LOXL1-AS1 Facilitates Colorectal Cancer Progression via Regulating miR-1224-5p/miR-761/HK2 Axis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T16%3A44%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Long%20Non-coding%20RNA%20LOXL1-AS1%20Facilitates%20Colorectal%20Cancer%20Progression%20via%20Regulating%20miR-1224-5p/miR-761/HK2%20Axis&rft.jtitle=Biochemical%20genetics&rft.au=Guo,%20Tao&rft.date=2022-12-01&rft.volume=60&rft.issue=6&rft.spage=2416&rft.epage=2433&rft.pages=2416-2433&rft.issn=0006-2928&rft.eissn=1573-4927&rft_id=info:doi/10.1007/s10528-022-10226-3&rft_dat=%3Cproquest_cross%3E2729996817%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2729996817&rft_id=info:pmid/35441953&rfr_iscdi=true |