Transcytosis of tanycytes in the circumventricular organs of adult mouse brain
•Tanycytic endocytosis and exocytosis of CTB and WGA in circumventricular organs.•Transport of CSF-injected CTB and WGA to parenchyma neurons.•Long processes are beneficial to transport CSF molecules to discrete neurons. Tanycytes are specialized ependymal cells lining the ventricular spaces of the...
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Veröffentlicht in: | Neuroscience letters 2022-05, Vol.779, p.136633-136633, Article 136633 |
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creator | Okamoto, Akihiro Fujii, Rena Yoshimura, Ryoichi Miyata, Seiji |
description | •Tanycytic endocytosis and exocytosis of CTB and WGA in circumventricular organs.•Transport of CSF-injected CTB and WGA to parenchyma neurons.•Long processes are beneficial to transport CSF molecules to discrete neurons.
Tanycytes are specialized ependymal cells lining the ventricular spaces of the adult brain and thereby provide an interface between the cerebrospinal fluid (CSF) and brain parenchyma. They act as energy homeostasis, neuroendocrine regulation, and CSF-brain barrier; however, their functional significance in CSF-brain communication currently remains unknown. In the present study, we investigated the presence of tanycytic transcytosis using fluorescent tracers; a GM1 ligand, cholera toxin B (CTB), and a mannose-6-phosphate/insulin-like growth factor-Ⅱ receptor ligand, wheat germ agglutinin (WGA). Both CTB and WGA were incorporated by tanycytes and then released into brain parenchyma in the circumventricular organs such as the organum vasculosum laminae terminalis, subfornical organ, and median eminence, arcuate nucleus, and medullary central canal. Incorporated fluorescent CTB and WGA were released from tanycytes to distribute at neuronal somata. These results indicate that tanycytes of all examined brain regions possess the transport capability of macromolecules from CSF to brain neurons. |
doi_str_mv | 10.1016/j.neulet.2022.136633 |
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Tanycytes are specialized ependymal cells lining the ventricular spaces of the adult brain and thereby provide an interface between the cerebrospinal fluid (CSF) and brain parenchyma. They act as energy homeostasis, neuroendocrine regulation, and CSF-brain barrier; however, their functional significance in CSF-brain communication currently remains unknown. In the present study, we investigated the presence of tanycytic transcytosis using fluorescent tracers; a GM1 ligand, cholera toxin B (CTB), and a mannose-6-phosphate/insulin-like growth factor-Ⅱ receptor ligand, wheat germ agglutinin (WGA). Both CTB and WGA were incorporated by tanycytes and then released into brain parenchyma in the circumventricular organs such as the organum vasculosum laminae terminalis, subfornical organ, and median eminence, arcuate nucleus, and medullary central canal. Incorporated fluorescent CTB and WGA were released from tanycytes to distribute at neuronal somata. These results indicate that tanycytes of all examined brain regions possess the transport capability of macromolecules from CSF to brain neurons.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2022.136633</identifier><identifier>PMID: 35429588</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Brain ; CSF ; CTB ; IGF- ; M6P ; WGA</subject><ispartof>Neuroscience letters, 2022-05, Vol.779, p.136633-136633, Article 136633</ispartof><rights>2022 Elsevier B.V.</rights><rights>Copyright © 2022 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-bc3d363e8e6179dc328e17593eea24a27780c6509a21d32cc0f894d3104c07c3</citedby><cites>FETCH-LOGICAL-c358t-bc3d363e8e6179dc328e17593eea24a27780c6509a21d32cc0f894d3104c07c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neulet.2022.136633$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35429588$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okamoto, Akihiro</creatorcontrib><creatorcontrib>Fujii, Rena</creatorcontrib><creatorcontrib>Yoshimura, Ryoichi</creatorcontrib><creatorcontrib>Miyata, Seiji</creatorcontrib><title>Transcytosis of tanycytes in the circumventricular organs of adult mouse brain</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>•Tanycytic endocytosis and exocytosis of CTB and WGA in circumventricular organs.•Transport of CSF-injected CTB and WGA to parenchyma neurons.•Long processes are beneficial to transport CSF molecules to discrete neurons.
Tanycytes are specialized ependymal cells lining the ventricular spaces of the adult brain and thereby provide an interface between the cerebrospinal fluid (CSF) and brain parenchyma. They act as energy homeostasis, neuroendocrine regulation, and CSF-brain barrier; however, their functional significance in CSF-brain communication currently remains unknown. In the present study, we investigated the presence of tanycytic transcytosis using fluorescent tracers; a GM1 ligand, cholera toxin B (CTB), and a mannose-6-phosphate/insulin-like growth factor-Ⅱ receptor ligand, wheat germ agglutinin (WGA). Both CTB and WGA were incorporated by tanycytes and then released into brain parenchyma in the circumventricular organs such as the organum vasculosum laminae terminalis, subfornical organ, and median eminence, arcuate nucleus, and medullary central canal. Incorporated fluorescent CTB and WGA were released from tanycytes to distribute at neuronal somata. These results indicate that tanycytes of all examined brain regions possess the transport capability of macromolecules from CSF to brain neurons.</description><subject>Brain</subject><subject>CSF</subject><subject>CTB</subject><subject>IGF-</subject><subject>M6P</subject><subject>WGA</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kMlOwzAQhi0EoqXwBgjlyCXBS-IkFySE2KQKLr1b7ngCrrIU26nUt8dVCkdOo5G-f5aPkGtGM0aZvNtkPY4thoxTzjMmpBTihMxZVfK0rEt-SuZU0DwVdU5n5ML7DaW0YEV-TmaiyHldVNWcvK-c7j3sw-CtT4YmCbrfxxZ9YvskfGEC1sHY7bAPzsLYapcM7jNmDrA2YxuSbhg9JmunbX9Jzhrderw61gVZPT-tHl_T5cfL2-PDMgVRVCFdgzBCCqxQsrI2IHiFrCxqgah5rnlZVhRkQWvNmREcgDZVnRvBaA60BLEgt9PYrRu-R_RBddYDtq3uMR6juCyYrOP3MqL5hIIbvHfYqK2znXZ7xag6iFQbNYlUB5FqEhljN8cN47pD8xf6NReB-wnA-ObOolMeLPaAxjqEoMxg_9_wA19KhnE</recordid><startdate>20220514</startdate><enddate>20220514</enddate><creator>Okamoto, Akihiro</creator><creator>Fujii, Rena</creator><creator>Yoshimura, Ryoichi</creator><creator>Miyata, Seiji</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20220514</creationdate><title>Transcytosis of tanycytes in the circumventricular organs of adult mouse brain</title><author>Okamoto, Akihiro ; Fujii, Rena ; Yoshimura, Ryoichi ; Miyata, Seiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-bc3d363e8e6179dc328e17593eea24a27780c6509a21d32cc0f894d3104c07c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Brain</topic><topic>CSF</topic><topic>CTB</topic><topic>IGF-</topic><topic>M6P</topic><topic>WGA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okamoto, Akihiro</creatorcontrib><creatorcontrib>Fujii, Rena</creatorcontrib><creatorcontrib>Yoshimura, Ryoichi</creatorcontrib><creatorcontrib>Miyata, Seiji</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okamoto, Akihiro</au><au>Fujii, Rena</au><au>Yoshimura, Ryoichi</au><au>Miyata, Seiji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcytosis of tanycytes in the circumventricular organs of adult mouse brain</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2022-05-14</date><risdate>2022</risdate><volume>779</volume><spage>136633</spage><epage>136633</epage><pages>136633-136633</pages><artnum>136633</artnum><issn>0304-3940</issn><eissn>1872-7972</eissn><abstract>•Tanycytic endocytosis and exocytosis of CTB and WGA in circumventricular organs.•Transport of CSF-injected CTB and WGA to parenchyma neurons.•Long processes are beneficial to transport CSF molecules to discrete neurons.
Tanycytes are specialized ependymal cells lining the ventricular spaces of the adult brain and thereby provide an interface between the cerebrospinal fluid (CSF) and brain parenchyma. They act as energy homeostasis, neuroendocrine regulation, and CSF-brain barrier; however, their functional significance in CSF-brain communication currently remains unknown. In the present study, we investigated the presence of tanycytic transcytosis using fluorescent tracers; a GM1 ligand, cholera toxin B (CTB), and a mannose-6-phosphate/insulin-like growth factor-Ⅱ receptor ligand, wheat germ agglutinin (WGA). Both CTB and WGA were incorporated by tanycytes and then released into brain parenchyma in the circumventricular organs such as the organum vasculosum laminae terminalis, subfornical organ, and median eminence, arcuate nucleus, and medullary central canal. Incorporated fluorescent CTB and WGA were released from tanycytes to distribute at neuronal somata. These results indicate that tanycytes of all examined brain regions possess the transport capability of macromolecules from CSF to brain neurons.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>35429588</pmid><doi>10.1016/j.neulet.2022.136633</doi><tpages>1</tpages></addata></record> |
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subjects | Brain CSF CTB IGF- M6P WGA |
title | Transcytosis of tanycytes in the circumventricular organs of adult mouse brain |
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