A tweak and a peek: How Cas9 pries open double-stranded DNA to check its sequence

The CRISPR–Cas enzyme Cas9 faces the challenge of identifying a specific nucleotide sequence within double-stranded DNA. New cryo-EM and biochemical studies show that in the earliest steps of binding, Cas9 bends the DNA and promotes unwinding of two base pairs, enabling it to efficiently scan the se...

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Veröffentlicht in:	Nature structural & molecular biology 2022-04, Vol.29 (4), p.286-288
Hauptverfasser:	Sinan, Selma, Russell, Rick
Format:	Artikel
Sprache:	eng
Schlagworte:	101/28 631/45/535 631/45/612/1242 Bends Binding sites Biochemistry Biological Microscopy Biomedical and Life Sciences CRISPR CRISPR-Cas Systems Deoxyribonucleic acid DNA Enzymes Life Sciences Membrane Biology Molecular biology News & Views news-and-views Nucleotide sequence Nucleotides Protein Structure Proteins Unwinding
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Beschreibung
Zusammenfassung:	The CRISPR–Cas enzyme Cas9 faces the challenge of identifying a specific nucleotide sequence within double-stranded DNA. New cryo-EM and biochemical studies show that in the earliest steps of binding, Cas9 bends the DNA and promotes unwinding of two base pairs, enabling it to efficiently scan the sequence of this critical region.
ISSN:	1545-9993 1545-9985
DOI:	10.1038/s41594-022-00763-1