Coupling Stable Isotope Labeling and Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight-Tandem Mass Spectrometry for De Novo Mosquito Ovarian Lipid Studies
There is a need to better understand lipid metabolism during mosquito ovarian development. Lipids are the major source of energy supporting ovarian follicles development in mosquitoes. In this paper, we describe the complementary use of stable isotope labeling (SIL) and high-resolution mass spectrom...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2022-04, Vol.94 (16), p.6139-6145 |
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| creator | Tose, Lilian V Ramirez, Cesar E Michalkova, Veronika Nouzova, Marcela Noriega, Fernando G Fernandez-Lima, Francisco |
| description | There is a need to better understand lipid metabolism during mosquito ovarian development. Lipids are the major source of energy supporting ovarian follicles development in mosquitoes. In this paper, we describe the complementary use of stable isotope labeling (SIL) and high-resolution mass spectrometry-based tools for the investigation of de novo triglycerides (TG) and diglycerides (DG) during the ovarian previtellogenic (PVG) stage (4–6 days posteclosion) of female adult Aedes aegypti. Liquid chromatography coupled to high-resolution trapped ion mobility spectrometry-parallel accumulation sequential fragmentation-time-of-flight tandem mass spectrometry (LC-TIMS-PASEF-TOF MS/MS) allowed the separation and quantification of nonlabeled and 2H/13C-labeled TG and DG species. Three SIL strategies were evaluated (H2O/2H2O with 50:50 and 95:5 mixtures, 13C-sucrose, and 13C-glucose). Results showed wide applicability with no signs of lipid ovarian impairment by SIL induced toxicity. The analytical workflow based on LC-TIMS-TOF MS/MS provided high confidence and high reproducibility for lipid DG and TG identification and SIL incorporation based on their separation by retention time (RT), collision cross section (CCS), and accurate m/z. In addition, the SIL fatty acid chain incorporation was evaluated using PASEF MS/MS. The 2H/13C incorporation into the mosquito diet provided information on how TG lipids are consumed, stored, and recycled during the PVG stage of ovarian development. |
| doi_str_mv | 10.1021/acs.analchem.1c05090 |
| format | Article |
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Lipids are the major source of energy supporting ovarian follicles development in mosquitoes. In this paper, we describe the complementary use of stable isotope labeling (SIL) and high-resolution mass spectrometry-based tools for the investigation of de novo triglycerides (TG) and diglycerides (DG) during the ovarian previtellogenic (PVG) stage (4–6 days posteclosion) of female adult Aedes aegypti. Liquid chromatography coupled to high-resolution trapped ion mobility spectrometry-parallel accumulation sequential fragmentation-time-of-flight tandem mass spectrometry (LC-TIMS-PASEF-TOF MS/MS) allowed the separation and quantification of nonlabeled and 2H/13C-labeled TG and DG species. Three SIL strategies were evaluated (H2O/2H2O with 50:50 and 95:5 mixtures, 13C-sucrose, and 13C-glucose). Results showed wide applicability with no signs of lipid ovarian impairment by SIL induced toxicity. The analytical workflow based on LC-TIMS-TOF MS/MS provided high confidence and high reproducibility for lipid DG and TG identification and SIL incorporation based on their separation by retention time (RT), collision cross section (CCS), and accurate m/z. In addition, the SIL fatty acid chain incorporation was evaluated using PASEF MS/MS. The 2H/13C incorporation into the mosquito diet provided information on how TG lipids are consumed, stored, and recycled during the PVG stage of ovarian development.</description><identifier>ISSN: 0003-2700</identifier><identifier>ISSN: 1520-6882</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.1c05090</identifier><identifier>PMID: 35420029</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Aedes aegypti ; Animals ; Aquatic insects ; Chemistry ; Chromatography ; Chromatography, Liquid ; Culicidae ; Diglycerides ; Diglycerides - analysis ; Diglycerides - chemistry ; Energy sources ; Fatty acids ; Female ; Follicles ; High resolution ; Incorporation ; Ion Mobility Spectrometry ; Ionic mobility ; Ions ; Isotope Labeling ; Labeling ; Lipid metabolism ; Lipids ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Mobility ; Mosquitoes ; Ovaries ; Reproducibility of Results ; Retention time ; Scientific imaging ; Separation ; Spectroscopy ; Stable isotopes ; Sucrose ; Tandem Mass Spectrometry - methods ; Toxicity ; Triglycerides ; Workflow</subject><ispartof>Analytical chemistry (Washington), 2022-04, Vol.94 (16), p.6139-6145</ispartof><rights>2022 American Chemical Society</rights><rights>Copyright American Chemical Society Apr 26, 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a352t-25e30a3dceb5a03e1b0bf24449aed44e0f3e7f1fbb549f3ab8d91052ec4adc613</citedby><cites>FETCH-LOGICAL-a352t-25e30a3dceb5a03e1b0bf24449aed44e0f3e7f1fbb549f3ab8d91052ec4adc613</cites><orcidid>0000-0002-1283-4390</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c05090$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.1c05090$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35420029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tose, Lilian V</creatorcontrib><creatorcontrib>Ramirez, Cesar E</creatorcontrib><creatorcontrib>Michalkova, Veronika</creatorcontrib><creatorcontrib>Nouzova, Marcela</creatorcontrib><creatorcontrib>Noriega, Fernando G</creatorcontrib><creatorcontrib>Fernandez-Lima, Francisco</creatorcontrib><title>Coupling Stable Isotope Labeling and Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight-Tandem Mass Spectrometry for De Novo Mosquito Ovarian Lipid Studies</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>There is a need to better understand lipid metabolism during mosquito ovarian development. Lipids are the major source of energy supporting ovarian follicles development in mosquitoes. In this paper, we describe the complementary use of stable isotope labeling (SIL) and high-resolution mass spectrometry-based tools for the investigation of de novo triglycerides (TG) and diglycerides (DG) during the ovarian previtellogenic (PVG) stage (4–6 days posteclosion) of female adult Aedes aegypti. Liquid chromatography coupled to high-resolution trapped ion mobility spectrometry-parallel accumulation sequential fragmentation-time-of-flight tandem mass spectrometry (LC-TIMS-PASEF-TOF MS/MS) allowed the separation and quantification of nonlabeled and 2H/13C-labeled TG and DG species. Three SIL strategies were evaluated (H2O/2H2O with 50:50 and 95:5 mixtures, 13C-sucrose, and 13C-glucose). Results showed wide applicability with no signs of lipid ovarian impairment by SIL induced toxicity. The analytical workflow based on LC-TIMS-TOF MS/MS provided high confidence and high reproducibility for lipid DG and TG identification and SIL incorporation based on their separation by retention time (RT), collision cross section (CCS), and accurate m/z. In addition, the SIL fatty acid chain incorporation was evaluated using PASEF MS/MS. The 2H/13C incorporation into the mosquito diet provided information on how TG lipids are consumed, stored, and recycled during the PVG stage of ovarian development.</description><subject>Aedes aegypti</subject><subject>Animals</subject><subject>Aquatic insects</subject><subject>Chemistry</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Culicidae</subject><subject>Diglycerides</subject><subject>Diglycerides - analysis</subject><subject>Diglycerides - chemistry</subject><subject>Energy sources</subject><subject>Fatty acids</subject><subject>Female</subject><subject>Follicles</subject><subject>High resolution</subject><subject>Incorporation</subject><subject>Ion Mobility Spectrometry</subject><subject>Ionic mobility</subject><subject>Ions</subject><subject>Isotope Labeling</subject><subject>Labeling</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Mobility</subject><subject>Mosquitoes</subject><subject>Ovaries</subject><subject>Reproducibility of Results</subject><subject>Retention time</subject><subject>Scientific imaging</subject><subject>Separation</subject><subject>Spectroscopy</subject><subject>Stable isotopes</subject><subject>Sucrose</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Toxicity</subject><subject>Triglycerides</subject><subject>Workflow</subject><issn>0003-2700</issn><issn>1520-6882</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u00AUhUcIREPhDRAaiQ0bhzs_TuIlCrRESukiYW3dsa-TqWyPOzOulKfiFTtp0kqwYHWlq--cs_gY-yhgKkCKr1iFKfbYVnvqpqKCHAp4xSYil5DNFgv5mk0AQGVyDnDB3oVwByAEiNlbdqFyLQFkMWF_lm4cWtvv-CaiaYmvgotuIL5GQ09_7Gu-tvejrfly712H0e08DvtDtk1noJqvXM9vnLGtjQe-GaiKCaPoE2E7ylyTXbV2t4_ZNnVRx28whL843jjPvxP_5R5cagppLDp--4DeYp_Gh7S9iWNtKbxnbxpsA30430v2--rHdvkzW99er5bf1hmqXMZM5qQAVV2RyREUCQOmkVrrAqnWmqBRNG9EY0yui0ahWdSFgFxSpbGuZkJdsi-n3sG7-5FCLDsbKmpb7MmNoZSzHGSuQaqEfv4HvXOjT2aeqLlWQs2PlD5RlXcheGrKwdsO_aEUUB6Flklo-Sy0PAtNsU_n8tF0VL-Eng0mAE7AMf4y_N_OR_hSszI</recordid><startdate>20220426</startdate><enddate>20220426</enddate><creator>Tose, Lilian V</creator><creator>Ramirez, Cesar E</creator><creator>Michalkova, Veronika</creator><creator>Nouzova, Marcela</creator><creator>Noriega, Fernando G</creator><creator>Fernandez-Lima, Francisco</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1283-4390</orcidid></search><sort><creationdate>20220426</creationdate><title>Coupling Stable Isotope Labeling and Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight-Tandem Mass Spectrometry for De Novo Mosquito Ovarian Lipid Studies</title><author>Tose, Lilian V ; 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Chem</addtitle><date>2022-04-26</date><risdate>2022</risdate><volume>94</volume><issue>16</issue><spage>6139</spage><epage>6145</epage><pages>6139-6145</pages><issn>0003-2700</issn><issn>1520-6882</issn><eissn>1520-6882</eissn><abstract>There is a need to better understand lipid metabolism during mosquito ovarian development. Lipids are the major source of energy supporting ovarian follicles development in mosquitoes. In this paper, we describe the complementary use of stable isotope labeling (SIL) and high-resolution mass spectrometry-based tools for the investigation of de novo triglycerides (TG) and diglycerides (DG) during the ovarian previtellogenic (PVG) stage (4–6 days posteclosion) of female adult Aedes aegypti. Liquid chromatography coupled to high-resolution trapped ion mobility spectrometry-parallel accumulation sequential fragmentation-time-of-flight tandem mass spectrometry (LC-TIMS-PASEF-TOF MS/MS) allowed the separation and quantification of nonlabeled and 2H/13C-labeled TG and DG species. Three SIL strategies were evaluated (H2O/2H2O with 50:50 and 95:5 mixtures, 13C-sucrose, and 13C-glucose). Results showed wide applicability with no signs of lipid ovarian impairment by SIL induced toxicity. The analytical workflow based on LC-TIMS-TOF MS/MS provided high confidence and high reproducibility for lipid DG and TG identification and SIL incorporation based on their separation by retention time (RT), collision cross section (CCS), and accurate m/z. In addition, the SIL fatty acid chain incorporation was evaluated using PASEF MS/MS. The 2H/13C incorporation into the mosquito diet provided information on how TG lipids are consumed, stored, and recycled during the PVG stage of ovarian development.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>35420029</pmid><doi>10.1021/acs.analchem.1c05090</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1283-4390</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Aedes aegypti Animals Aquatic insects Chemistry Chromatography Chromatography, Liquid Culicidae Diglycerides Diglycerides - analysis Diglycerides - chemistry Energy sources Fatty acids Female Follicles High resolution Incorporation Ion Mobility Spectrometry Ionic mobility Ions Isotope Labeling Labeling Lipid metabolism Lipids Liquid chromatography Mass spectrometry Mass spectroscopy Mobility Mosquitoes Ovaries Reproducibility of Results Retention time Scientific imaging Separation Spectroscopy Stable isotopes Sucrose Tandem Mass Spectrometry - methods Toxicity Triglycerides Workflow |
| title | Coupling Stable Isotope Labeling and Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight-Tandem Mass Spectrometry for De Novo Mosquito Ovarian Lipid Studies |
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