Fluorescent annulated imidazo[4,5- c ]isoquinolines via a GBB-3CR/imidoylation sequence - DNA-interactions in pUC-19 gel electrophoresis mobility shift assay
Herein we report the development of a sequential synthesis route towards annulated imidazo[4,5- ]isoquinolines comprising a GBB-3CR, followed by an intramolecular imidoylative cyclisation. X-Ray crystallography revealed a flat 3D structure of the obtained polyheterocycles. Thus, we evaluated their i...
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| Veröffentlicht in: | Organic & biomolecular chemistry 2022-05, Vol.20 (17), p.3598-3604 |
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| container_issue | 17 |
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| container_title | Organic & biomolecular chemistry |
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| creator | Stahlberger, M Steinlein, O Adam, C R Rotter, M Hohmann, J Nieger, M Köberle, B Bräse, S |
| description | Herein we report the development of a sequential synthesis route towards annulated imidazo[4,5-
]isoquinolines comprising a GBB-3CR, followed by an intramolecular imidoylative cyclisation. X-Ray crystallography revealed a flat 3D structure of the obtained polyheterocycles. Thus, we evaluated their interactions with double-stranded DNA by establishing a pUC-19 plasmid-based gel electrophoresis mobility shift assay, revealing a stabilising effect on ds-DNA against strand-break inducing conditions. |
| doi_str_mv | 10.1039/d2ob00372d |
| format | Article |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Crystallography Cyclization Deoxyribonucleic acid DNA DNA damage Electrophoresis Electrophoresis, Polyacrylamide Gel Electrophoretic mobility Electrophoretic Mobility Shift Assay Fluorescence Gel electrophoresis Isoquinolines - chemistry Mobility Nucleotide sequence Plasmids X-ray crystallography |
| title | Fluorescent annulated imidazo[4,5- c ]isoquinolines via a GBB-3CR/imidoylation sequence - DNA-interactions in pUC-19 gel electrophoresis mobility shift assay |
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