Punicalagin Attenuates Neuronal Apoptosis by Upregulating 5‑Hydroxymethylcytosine in the Diabetic Mouse Brain

Punicalagin exerts neuroprotective activity by improving AMP-activated kinase (AMPK) and mitochondrial Krebs cycle. AMPK and Krebs cycle metabolites regulate 5-hydroxymethylcytosine (5hmC) via acting on ten-eleven translocation (TET) enzymes. Therefore, we hypothesized that punicalagin inhibits diab...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of agricultural and food chemistry 2022-04, Vol.70 (16), p.4995-5004
Hauptverfasser: He, Xin, Pei, Shengjie, Meng, Xiangyuan, Hua, Qinglian, Zhang, Tianyu, Wang, Yan, Zhang, Zhizhao, Zhu, Xinyu, Liu, Run, Guo, Yurong, Chen, Lei, Li, Duo
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 5004
container_issue 16
container_start_page 4995
container_title Journal of agricultural and food chemistry
container_volume 70
creator He, Xin
Pei, Shengjie
Meng, Xiangyuan
Hua, Qinglian
Zhang, Tianyu
Wang, Yan
Zhang, Zhizhao
Zhu, Xinyu
Liu, Run
Guo, Yurong
Chen, Lei
Li, Duo
description Punicalagin exerts neuroprotective activity by improving AMP-activated kinase (AMPK) and mitochondrial Krebs cycle. AMPK and Krebs cycle metabolites regulate 5-hydroxymethylcytosine (5hmC) via acting on ten-eleven translocation (TET) enzymes. Therefore, we hypothesized that punicalagin inhibits diabetes-related neuronal apoptosis by upregulating 5hmC in the diabetic mouse brain. C57BL/6J mice aged 8 weeks were randomly separated into five groups (n = 10), normal control (NC), diabetes mellitus (DM), resveratrol (RES), low-dose punicalagin (LPU), and high-dose punicalagin (HPU). Compared with other groups, the neuronal apoptosis rate was significantly higher and the 5hmC level of the cerebral cortex was significantly lower in the DM group. The levels of TET2 and P-AMPKα/AMPKα were significantly lower in the DM group than in both LPU and HPU groups. The ratio of (succinic acid + fumaric acid)/α-ketoglutarate was significantly higher in the DM group than in other groups. The present results suggest that punicalagin upregulates 5hmC via activating AMPK and maintaining Krebs cycle homeostasis, thus inhibiting neuronal apoptosis in the diabetic mouse brain.
doi_str_mv 10.1021/acs.jafc.2c00863
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2649999723</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2649999723</sourcerecordid><originalsourceid>FETCH-LOGICAL-a336t-b5b6d561f46508957496046fdf3704b16773ca750a488c7a978dd85092aa69833</originalsourceid><addsrcrecordid>eNp1kLtO7DAURS10EQyPngq5pCCDncSPlMMbiVcBdXTiOINRJg62I5GOX-AX-RI8zEB3T3Oatbe2FkIHlEwpSekJKD99hUZNU0WI5NkGmlCWkoRRKv-hCYlMIhmn22jH-1cSGSbIFtrOWE5TmRYTZB-HzihoYW46PAtBdwME7fG9HpztoMWz3vbBeuNxNeLn3un50EIw3Ryzr4_P67F29n1c6PAytmpcgp3GsSq8aHxuoNLBKHxnB6_xqQPT7aHNBlqv99d_Fz1fXjydXSe3D1c3Z7PbBLKMh6RiFa_j8CbnjMiCibzgJOdN3WSC5BXlQmQKBCOQS6kEFELWtWSkSAF4IbNsFx2tentn3wbtQ7kwXum2hU7HNWXK8yKeSJcoWaHKWe-dbsremQW4saSkXGouo-Zyqblca46Rw3X7UC10_Rf49RqB4xXwE7WDiyr9__u-AW21iqU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2649999723</pqid></control><display><type>article</type><title>Punicalagin Attenuates Neuronal Apoptosis by Upregulating 5‑Hydroxymethylcytosine in the Diabetic Mouse Brain</title><source>MEDLINE</source><source>ACS Publications</source><creator>He, Xin ; Pei, Shengjie ; Meng, Xiangyuan ; Hua, Qinglian ; Zhang, Tianyu ; Wang, Yan ; Zhang, Zhizhao ; Zhu, Xinyu ; Liu, Run ; Guo, Yurong ; Chen, Lei ; Li, Duo</creator><creatorcontrib>He, Xin ; Pei, Shengjie ; Meng, Xiangyuan ; Hua, Qinglian ; Zhang, Tianyu ; Wang, Yan ; Zhang, Zhizhao ; Zhu, Xinyu ; Liu, Run ; Guo, Yurong ; Chen, Lei ; Li, Duo</creatorcontrib><description>Punicalagin exerts neuroprotective activity by improving AMP-activated kinase (AMPK) and mitochondrial Krebs cycle. AMPK and Krebs cycle metabolites regulate 5-hydroxymethylcytosine (5hmC) via acting on ten-eleven translocation (TET) enzymes. Therefore, we hypothesized that punicalagin inhibits diabetes-related neuronal apoptosis by upregulating 5hmC in the diabetic mouse brain. C57BL/6J mice aged 8 weeks were randomly separated into five groups (n = 10), normal control (NC), diabetes mellitus (DM), resveratrol (RES), low-dose punicalagin (LPU), and high-dose punicalagin (HPU). Compared with other groups, the neuronal apoptosis rate was significantly higher and the 5hmC level of the cerebral cortex was significantly lower in the DM group. The levels of TET2 and P-AMPKα/AMPKα were significantly lower in the DM group than in both LPU and HPU groups. The ratio of (succinic acid + fumaric acid)/α-ketoglutarate was significantly higher in the DM group than in other groups. The present results suggest that punicalagin upregulates 5hmC via activating AMPK and maintaining Krebs cycle homeostasis, thus inhibiting neuronal apoptosis in the diabetic mouse brain.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.2c00863</identifier><identifier>PMID: 35412829</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>5-Methylcytosine - analogs &amp; derivatives ; AMP-Activated Protein Kinases - genetics ; AMP-Activated Protein Kinases - metabolism ; Animals ; Apoptosis ; Bioactive Constituents, Metabolites, and Functions ; Brain - metabolism ; Diabetes Mellitus - metabolism ; Hydrolyzable Tannins ; Mice ; Mice, Inbred C57BL</subject><ispartof>Journal of agricultural and food chemistry, 2022-04, Vol.70 (16), p.4995-5004</ispartof><rights>2022 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-b5b6d561f46508957496046fdf3704b16773ca750a488c7a978dd85092aa69833</citedby><cites>FETCH-LOGICAL-a336t-b5b6d561f46508957496046fdf3704b16773ca750a488c7a978dd85092aa69833</cites><orcidid>0000-0002-6407-5293 ; 0000-0001-5227-5565</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.2c00863$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.2c00863$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27078,27926,27927,56740,56790</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35412829$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Xin</creatorcontrib><creatorcontrib>Pei, Shengjie</creatorcontrib><creatorcontrib>Meng, Xiangyuan</creatorcontrib><creatorcontrib>Hua, Qinglian</creatorcontrib><creatorcontrib>Zhang, Tianyu</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhang, Zhizhao</creatorcontrib><creatorcontrib>Zhu, Xinyu</creatorcontrib><creatorcontrib>Liu, Run</creatorcontrib><creatorcontrib>Guo, Yurong</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Li, Duo</creatorcontrib><title>Punicalagin Attenuates Neuronal Apoptosis by Upregulating 5‑Hydroxymethylcytosine in the Diabetic Mouse Brain</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Punicalagin exerts neuroprotective activity by improving AMP-activated kinase (AMPK) and mitochondrial Krebs cycle. AMPK and Krebs cycle metabolites regulate 5-hydroxymethylcytosine (5hmC) via acting on ten-eleven translocation (TET) enzymes. Therefore, we hypothesized that punicalagin inhibits diabetes-related neuronal apoptosis by upregulating 5hmC in the diabetic mouse brain. C57BL/6J mice aged 8 weeks were randomly separated into five groups (n = 10), normal control (NC), diabetes mellitus (DM), resveratrol (RES), low-dose punicalagin (LPU), and high-dose punicalagin (HPU). Compared with other groups, the neuronal apoptosis rate was significantly higher and the 5hmC level of the cerebral cortex was significantly lower in the DM group. The levels of TET2 and P-AMPKα/AMPKα were significantly lower in the DM group than in both LPU and HPU groups. The ratio of (succinic acid + fumaric acid)/α-ketoglutarate was significantly higher in the DM group than in other groups. The present results suggest that punicalagin upregulates 5hmC via activating AMPK and maintaining Krebs cycle homeostasis, thus inhibiting neuronal apoptosis in the diabetic mouse brain.</description><subject>5-Methylcytosine - analogs &amp; derivatives</subject><subject>AMP-Activated Protein Kinases - genetics</subject><subject>AMP-Activated Protein Kinases - metabolism</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Brain - metabolism</subject><subject>Diabetes Mellitus - metabolism</subject><subject>Hydrolyzable Tannins</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLtO7DAURS10EQyPngq5pCCDncSPlMMbiVcBdXTiOINRJg62I5GOX-AX-RI8zEB3T3Oatbe2FkIHlEwpSekJKD99hUZNU0WI5NkGmlCWkoRRKv-hCYlMIhmn22jH-1cSGSbIFtrOWE5TmRYTZB-HzihoYW46PAtBdwME7fG9HpztoMWz3vbBeuNxNeLn3un50EIw3Ryzr4_P67F29n1c6PAytmpcgp3GsSq8aHxuoNLBKHxnB6_xqQPT7aHNBlqv99d_Fz1fXjydXSe3D1c3Z7PbBLKMh6RiFa_j8CbnjMiCibzgJOdN3WSC5BXlQmQKBCOQS6kEFELWtWSkSAF4IbNsFx2tentn3wbtQ7kwXum2hU7HNWXK8yKeSJcoWaHKWe-dbsremQW4saSkXGouo-Zyqblca46Rw3X7UC10_Rf49RqB4xXwE7WDiyr9__u-AW21iqU</recordid><startdate>20220427</startdate><enddate>20220427</enddate><creator>He, Xin</creator><creator>Pei, Shengjie</creator><creator>Meng, Xiangyuan</creator><creator>Hua, Qinglian</creator><creator>Zhang, Tianyu</creator><creator>Wang, Yan</creator><creator>Zhang, Zhizhao</creator><creator>Zhu, Xinyu</creator><creator>Liu, Run</creator><creator>Guo, Yurong</creator><creator>Chen, Lei</creator><creator>Li, Duo</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6407-5293</orcidid><orcidid>https://orcid.org/0000-0001-5227-5565</orcidid></search><sort><creationdate>20220427</creationdate><title>Punicalagin Attenuates Neuronal Apoptosis by Upregulating 5‑Hydroxymethylcytosine in the Diabetic Mouse Brain</title><author>He, Xin ; Pei, Shengjie ; Meng, Xiangyuan ; Hua, Qinglian ; Zhang, Tianyu ; Wang, Yan ; Zhang, Zhizhao ; Zhu, Xinyu ; Liu, Run ; Guo, Yurong ; Chen, Lei ; Li, Duo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-b5b6d561f46508957496046fdf3704b16773ca750a488c7a978dd85092aa69833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>5-Methylcytosine - analogs &amp; derivatives</topic><topic>AMP-Activated Protein Kinases - genetics</topic><topic>AMP-Activated Protein Kinases - metabolism</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Brain - metabolism</topic><topic>Diabetes Mellitus - metabolism</topic><topic>Hydrolyzable Tannins</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Xin</creatorcontrib><creatorcontrib>Pei, Shengjie</creatorcontrib><creatorcontrib>Meng, Xiangyuan</creatorcontrib><creatorcontrib>Hua, Qinglian</creatorcontrib><creatorcontrib>Zhang, Tianyu</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhang, Zhizhao</creatorcontrib><creatorcontrib>Zhu, Xinyu</creatorcontrib><creatorcontrib>Liu, Run</creatorcontrib><creatorcontrib>Guo, Yurong</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Li, Duo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Xin</au><au>Pei, Shengjie</au><au>Meng, Xiangyuan</au><au>Hua, Qinglian</au><au>Zhang, Tianyu</au><au>Wang, Yan</au><au>Zhang, Zhizhao</au><au>Zhu, Xinyu</au><au>Liu, Run</au><au>Guo, Yurong</au><au>Chen, Lei</au><au>Li, Duo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Punicalagin Attenuates Neuronal Apoptosis by Upregulating 5‑Hydroxymethylcytosine in the Diabetic Mouse Brain</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2022-04-27</date><risdate>2022</risdate><volume>70</volume><issue>16</issue><spage>4995</spage><epage>5004</epage><pages>4995-5004</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Punicalagin exerts neuroprotective activity by improving AMP-activated kinase (AMPK) and mitochondrial Krebs cycle. AMPK and Krebs cycle metabolites regulate 5-hydroxymethylcytosine (5hmC) via acting on ten-eleven translocation (TET) enzymes. Therefore, we hypothesized that punicalagin inhibits diabetes-related neuronal apoptosis by upregulating 5hmC in the diabetic mouse brain. C57BL/6J mice aged 8 weeks were randomly separated into five groups (n = 10), normal control (NC), diabetes mellitus (DM), resveratrol (RES), low-dose punicalagin (LPU), and high-dose punicalagin (HPU). Compared with other groups, the neuronal apoptosis rate was significantly higher and the 5hmC level of the cerebral cortex was significantly lower in the DM group. The levels of TET2 and P-AMPKα/AMPKα were significantly lower in the DM group than in both LPU and HPU groups. The ratio of (succinic acid + fumaric acid)/α-ketoglutarate was significantly higher in the DM group than in other groups. The present results suggest that punicalagin upregulates 5hmC via activating AMPK and maintaining Krebs cycle homeostasis, thus inhibiting neuronal apoptosis in the diabetic mouse brain.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>35412829</pmid><doi>10.1021/acs.jafc.2c00863</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-6407-5293</orcidid><orcidid>https://orcid.org/0000-0001-5227-5565</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0021-8561
ispartof Journal of agricultural and food chemistry, 2022-04, Vol.70 (16), p.4995-5004
issn 0021-8561
1520-5118
language eng
recordid cdi_proquest_miscellaneous_2649999723
source MEDLINE; ACS Publications
subjects 5-Methylcytosine - analogs & derivatives
AMP-Activated Protein Kinases - genetics
AMP-Activated Protein Kinases - metabolism
Animals
Apoptosis
Bioactive Constituents, Metabolites, and Functions
Brain - metabolism
Diabetes Mellitus - metabolism
Hydrolyzable Tannins
Mice
Mice, Inbred C57BL
title Punicalagin Attenuates Neuronal Apoptosis by Upregulating 5‑Hydroxymethylcytosine in the Diabetic Mouse Brain
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T05%3A52%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Punicalagin%20Attenuates%20Neuronal%20Apoptosis%20by%20Upregulating%205%E2%80%91Hydroxymethylcytosine%20in%20the%20Diabetic%20Mouse%20Brain&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=He,%20Xin&rft.date=2022-04-27&rft.volume=70&rft.issue=16&rft.spage=4995&rft.epage=5004&rft.pages=4995-5004&rft.issn=0021-8561&rft.eissn=1520-5118&rft_id=info:doi/10.1021/acs.jafc.2c00863&rft_dat=%3Cproquest_cross%3E2649999723%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2649999723&rft_id=info:pmid/35412829&rfr_iscdi=true