Oct4 dependent chromatin activation is required for chicken primordial germ cell migration
Primordial germ cells (PGCs) are the undifferentiated progenitors of the gametes. Unlike the poor maintenance of cultured mammalian PGCs, the avian PGCs can be expanded in vitro indefinitely while preserving pluripotency and germline competence. In mammals, the Oct4 is the master transcription facto...
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| Veröffentlicht in: | Stem cell reviews and reports 2022-10, Vol.18 (7), p.2535-2546 |
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| creator | Meng, Lu Wang, Sheng Jiang, Haoyi Hua, Yao Yin, Binxu Huang, Xiaochen Man, Qiu Wang, Heng Zhu, Guiyu |
| description | Primordial germ cells (PGCs) are the undifferentiated progenitors of the gametes. Unlike the poor maintenance of cultured mammalian PGCs, the avian PGCs can be expanded
in vitro
indefinitely while preserving pluripotency and germline competence. In mammals, the Oct4 is the master transcription factor that ensures the stemness of pluripotent cells such as PGCs, but the specific function of Oct4 in chicken PGCs remains unclear. As expected, the loss of Oct4 in chicken PGCs reduced the expression of key pluripotency factors and promoted the genes involved in endoderm and ectoderm differentiation. Furthermore, the global active chromatin was reduced as shown by the depletion of the H3K27ac upon Oct4 suppression. Interestingly, the de-activated chromatin caused the down-regulation of adjacent genes which are mostly known regulators of cell junction, chemotaxis and cell migration. Consequently, the Oct4-deficient PGCs show impaired cell migration and could not colonize the gonads when re-introduced into the bloodstream of the embryo. We propose that, in addition to maintaining pluripotency, the Oct4 mediated chromatin activation is dictating chicken PGC migration.
Graphical abstract |
| doi_str_mv | 10.1007/s12015-022-10371-7 |
| format | Article |
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in vitro
indefinitely while preserving pluripotency and germline competence. In mammals, the Oct4 is the master transcription factor that ensures the stemness of pluripotent cells such as PGCs, but the specific function of Oct4 in chicken PGCs remains unclear. As expected, the loss of Oct4 in chicken PGCs reduced the expression of key pluripotency factors and promoted the genes involved in endoderm and ectoderm differentiation. Furthermore, the global active chromatin was reduced as shown by the depletion of the H3K27ac upon Oct4 suppression. Interestingly, the de-activated chromatin caused the down-regulation of adjacent genes which are mostly known regulators of cell junction, chemotaxis and cell migration. Consequently, the Oct4-deficient PGCs show impaired cell migration and could not colonize the gonads when re-introduced into the bloodstream of the embryo. We propose that, in addition to maintaining pluripotency, the Oct4 mediated chromatin activation is dictating chicken PGC migration.
Graphical abstract</description><identifier>ISSN: 2629-3269</identifier><identifier>EISSN: 2629-3277</identifier><identifier>DOI: 10.1007/s12015-022-10371-7</identifier><identifier>PMID: 35397052</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Cell adhesion & migration ; Cell Biology ; Cell migration ; Chemotaxis ; Chromatin ; Ectoderm ; Endoderm ; Gametes ; Gene regulation ; Germ cells ; Gonads ; Life Sciences ; Mammals ; Oct-4 protein ; Pluripotency ; Progenitor cells ; Regenerative Medicine/Tissue Engineering ; Stem Cells</subject><ispartof>Stem cell reviews and reports, 2022-10, Vol.18 (7), p.2535-2546</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-9198b09ae7f831b72011e23719bdfe10204f874332abda14faa9bdfb7c04e663</citedby><cites>FETCH-LOGICAL-c371t-9198b09ae7f831b72011e23719bdfe10204f874332abda14faa9bdfb7c04e663</cites><orcidid>0000-0001-8440-8852</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12015-022-10371-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12015-022-10371-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35397052$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meng, Lu</creatorcontrib><creatorcontrib>Wang, Sheng</creatorcontrib><creatorcontrib>Jiang, Haoyi</creatorcontrib><creatorcontrib>Hua, Yao</creatorcontrib><creatorcontrib>Yin, Binxu</creatorcontrib><creatorcontrib>Huang, Xiaochen</creatorcontrib><creatorcontrib>Man, Qiu</creatorcontrib><creatorcontrib>Wang, Heng</creatorcontrib><creatorcontrib>Zhu, Guiyu</creatorcontrib><title>Oct4 dependent chromatin activation is required for chicken primordial germ cell migration</title><title>Stem cell reviews and reports</title><addtitle>Stem Cell Rev and Rep</addtitle><addtitle>Stem Cell Rev Rep</addtitle><description>Primordial germ cells (PGCs) are the undifferentiated progenitors of the gametes. Unlike the poor maintenance of cultured mammalian PGCs, the avian PGCs can be expanded
in vitro
indefinitely while preserving pluripotency and germline competence. In mammals, the Oct4 is the master transcription factor that ensures the stemness of pluripotent cells such as PGCs, but the specific function of Oct4 in chicken PGCs remains unclear. As expected, the loss of Oct4 in chicken PGCs reduced the expression of key pluripotency factors and promoted the genes involved in endoderm and ectoderm differentiation. Furthermore, the global active chromatin was reduced as shown by the depletion of the H3K27ac upon Oct4 suppression. Interestingly, the de-activated chromatin caused the down-regulation of adjacent genes which are mostly known regulators of cell junction, chemotaxis and cell migration. Consequently, the Oct4-deficient PGCs show impaired cell migration and could not colonize the gonads when re-introduced into the bloodstream of the embryo. We propose that, in addition to maintaining pluripotency, the Oct4 mediated chromatin activation is dictating chicken PGC migration.
Graphical abstract</description><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Cell adhesion & migration</subject><subject>Cell Biology</subject><subject>Cell migration</subject><subject>Chemotaxis</subject><subject>Chromatin</subject><subject>Ectoderm</subject><subject>Endoderm</subject><subject>Gametes</subject><subject>Gene regulation</subject><subject>Germ cells</subject><subject>Gonads</subject><subject>Life Sciences</subject><subject>Mammals</subject><subject>Oct-4 protein</subject><subject>Pluripotency</subject><subject>Progenitor cells</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Stem Cells</subject><issn>2629-3269</issn><issn>2629-3277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kDtPwzAUhS0EolXpH2BAllhYAn4ljkdU8ZIqsXRisZzkprjk0doJEv8epylFYmDyle53js89CF1ScksJkXeeMkLjiDAWUcIljeQJmrKEqYgzKU-Pc6ImaO79hhDCOBFBc44mPOZKkphN0dtr3glcwBaaApoO5--urU1nG2zyzn6GqW2w9djBrrcOCly2LkA2_4AGb52tW1dYU-E1uBrnUFW4tmu3l12gs9JUHuaHd4ZWjw-rxXO0fH16WdwvozzE7iJFVZoRZUCWKaeZDGdRYGGlsqIEShgRZSoF58xkhaGiNGbYZDInApKEz9DNaLt17a4H3-na-iGJaaDtvWaJSFOlBJMBvf6DbtreNSGcZpLGigqVxIFiI5W71nsHpR7uNO5LU6KH7vXYvQ7d6333erC-Olj3WQ3FUfLTdAD4CPiwakJdv3__Y_sNiAqOig</recordid><startdate>20221001</startdate><enddate>20221001</enddate><creator>Meng, Lu</creator><creator>Wang, Sheng</creator><creator>Jiang, Haoyi</creator><creator>Hua, Yao</creator><creator>Yin, Binxu</creator><creator>Huang, Xiaochen</creator><creator>Man, Qiu</creator><creator>Wang, Heng</creator><creator>Zhu, Guiyu</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8440-8852</orcidid></search><sort><creationdate>20221001</creationdate><title>Oct4 dependent chromatin activation is required for chicken primordial germ cell migration</title><author>Meng, Lu ; Wang, Sheng ; Jiang, Haoyi ; Hua, Yao ; Yin, Binxu ; Huang, Xiaochen ; Man, Qiu ; Wang, Heng ; Zhu, Guiyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-9198b09ae7f831b72011e23719bdfe10204f874332abda14faa9bdfb7c04e663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Cell adhesion & migration</topic><topic>Cell Biology</topic><topic>Cell migration</topic><topic>Chemotaxis</topic><topic>Chromatin</topic><topic>Ectoderm</topic><topic>Endoderm</topic><topic>Gametes</topic><topic>Gene regulation</topic><topic>Germ cells</topic><topic>Gonads</topic><topic>Life Sciences</topic><topic>Mammals</topic><topic>Oct-4 protein</topic><topic>Pluripotency</topic><topic>Progenitor cells</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Stem Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meng, Lu</creatorcontrib><creatorcontrib>Wang, Sheng</creatorcontrib><creatorcontrib>Jiang, Haoyi</creatorcontrib><creatorcontrib>Hua, Yao</creatorcontrib><creatorcontrib>Yin, Binxu</creatorcontrib><creatorcontrib>Huang, Xiaochen</creatorcontrib><creatorcontrib>Man, Qiu</creatorcontrib><creatorcontrib>Wang, Heng</creatorcontrib><creatorcontrib>Zhu, Guiyu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Stem cell reviews and reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meng, Lu</au><au>Wang, Sheng</au><au>Jiang, Haoyi</au><au>Hua, Yao</au><au>Yin, Binxu</au><au>Huang, Xiaochen</au><au>Man, Qiu</au><au>Wang, Heng</au><au>Zhu, Guiyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oct4 dependent chromatin activation is required for chicken primordial germ cell migration</atitle><jtitle>Stem cell reviews and reports</jtitle><stitle>Stem Cell Rev and Rep</stitle><addtitle>Stem Cell Rev Rep</addtitle><date>2022-10-01</date><risdate>2022</risdate><volume>18</volume><issue>7</issue><spage>2535</spage><epage>2546</epage><pages>2535-2546</pages><issn>2629-3269</issn><eissn>2629-3277</eissn><abstract>Primordial germ cells (PGCs) are the undifferentiated progenitors of the gametes. Unlike the poor maintenance of cultured mammalian PGCs, the avian PGCs can be expanded
in vitro
indefinitely while preserving pluripotency and germline competence. In mammals, the Oct4 is the master transcription factor that ensures the stemness of pluripotent cells such as PGCs, but the specific function of Oct4 in chicken PGCs remains unclear. As expected, the loss of Oct4 in chicken PGCs reduced the expression of key pluripotency factors and promoted the genes involved in endoderm and ectoderm differentiation. Furthermore, the global active chromatin was reduced as shown by the depletion of the H3K27ac upon Oct4 suppression. Interestingly, the de-activated chromatin caused the down-regulation of adjacent genes which are mostly known regulators of cell junction, chemotaxis and cell migration. Consequently, the Oct4-deficient PGCs show impaired cell migration and could not colonize the gonads when re-introduced into the bloodstream of the embryo. We propose that, in addition to maintaining pluripotency, the Oct4 mediated chromatin activation is dictating chicken PGC migration.
Graphical abstract</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35397052</pmid><doi>10.1007/s12015-022-10371-7</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-8440-8852</orcidid></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedical Engineering and Bioengineering Cell adhesion & migration Cell Biology Cell migration Chemotaxis Chromatin Ectoderm Endoderm Gametes Gene regulation Germ cells Gonads Life Sciences Mammals Oct-4 protein Pluripotency Progenitor cells Regenerative Medicine/Tissue Engineering Stem Cells |
| title | Oct4 dependent chromatin activation is required for chicken primordial germ cell migration |
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