Purification of mammalian telomeric DNA for single-molecule analysis

Here we provide a detailed protocol for the enrichment of telomeric repeats from mouse and human cells. The procedure consists of two successive rounds of digestion with frequently cutting restriction enzymes followed by size fractionation. Around 2 mg of genomic DNA is required, and the procedure l...

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Veröffentlicht in:	Nature protocols 2022-06, Vol.17 (6), p.1444-1467
Hauptverfasser:	Mazzucco, Giulia, Huda, Armela, Galli, Martina, Zanella, Elia, Doksani, Ylli
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2230 631/337/151/2357 631/337/1644 631/45/147 Analytical Chemistry Biological Techniques Biomedical and Life Sciences Computational Biology/Bioinformatics Deoxyribonucleic acid Digestion DNA DNA sequencing Electron microscopy Enrichment Enzymes Fractionation Intermediates Life Sciences Microarrays Molecular structure Organic Chemistry Protocol Purification Recombination Sequence analysis Telomeres
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